Job ID = 1293325 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 29,133,963 reads read : 29,133,963 reads written : 29,133,963 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:45 29133963 reads; of these: 29133963 (100.00%) were unpaired; of these: 2095801 (7.19%) aligned 0 times 24880827 (85.40%) aligned exactly 1 time 2157335 (7.40%) aligned >1 times 92.81% overall alignment rate Time searching: 00:09:45 Overall time: 00:09:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4818847 / 27038162 = 0.1782 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 23:16:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:16:23: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:16:23: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:16:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:16:23: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:16:23: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:16:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:16:23: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:16:23: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:16:32: 1000000 INFO @ Sun, 02 Jun 2019 23:16:33: 1000000 INFO @ Sun, 02 Jun 2019 23:16:34: 1000000 INFO @ Sun, 02 Jun 2019 23:16:40: 2000000 INFO @ Sun, 02 Jun 2019 23:16:41: 2000000 INFO @ Sun, 02 Jun 2019 23:16:44: 2000000 INFO @ Sun, 02 Jun 2019 23:16:49: 3000000 INFO @ Sun, 02 Jun 2019 23:16:50: 3000000 INFO @ Sun, 02 Jun 2019 23:16:55: 3000000 INFO @ Sun, 02 Jun 2019 23:16:58: 4000000 INFO @ Sun, 02 Jun 2019 23:16:59: 4000000 INFO @ Sun, 02 Jun 2019 23:17:05: 4000000 INFO @ Sun, 02 Jun 2019 23:17:08: 5000000 INFO @ Sun, 02 Jun 2019 23:17:08: 5000000 INFO @ Sun, 02 Jun 2019 23:17:16: 5000000 INFO @ Sun, 02 Jun 2019 23:17:16: 6000000 INFO @ Sun, 02 Jun 2019 23:17:18: 6000000 INFO @ Sun, 02 Jun 2019 23:17:25: 7000000 INFO @ Sun, 02 Jun 2019 23:17:25: 6000000 INFO @ Sun, 02 Jun 2019 23:17:28: 7000000 INFO @ Sun, 02 Jun 2019 23:17:33: 8000000 INFO @ Sun, 02 Jun 2019 23:17:35: 7000000 INFO @ Sun, 02 Jun 2019 23:17:38: 8000000 INFO @ Sun, 02 Jun 2019 23:17:42: 9000000 INFO @ Sun, 02 Jun 2019 23:17:45: 8000000 INFO @ Sun, 02 Jun 2019 23:17:48: 9000000 INFO @ Sun, 02 Jun 2019 23:17:50: 10000000 INFO @ Sun, 02 Jun 2019 23:17:55: 9000000 INFO @ Sun, 02 Jun 2019 23:17:58: 10000000 INFO @ Sun, 02 Jun 2019 23:17:59: 11000000 INFO @ Sun, 02 Jun 2019 23:18:05: 10000000 INFO @ Sun, 02 Jun 2019 23:18:07: 12000000 INFO @ Sun, 02 Jun 2019 23:18:08: 11000000 INFO @ Sun, 02 Jun 2019 23:18:15: 11000000 INFO @ Sun, 02 Jun 2019 23:18:16: 13000000 INFO @ Sun, 02 Jun 2019 23:18:18: 12000000 INFO @ Sun, 02 Jun 2019 23:18:25: 14000000 INFO @ Sun, 02 Jun 2019 23:18:25: 12000000 INFO @ Sun, 02 Jun 2019 23:18:28: 13000000 INFO @ Sun, 02 Jun 2019 23:18:33: 15000000 INFO @ Sun, 02 Jun 2019 23:18:35: 13000000 INFO @ Sun, 02 Jun 2019 23:18:38: 14000000 INFO @ Sun, 02 Jun 2019 23:18:42: 16000000 INFO @ Sun, 02 Jun 2019 23:18:45: 14000000 INFO @ Sun, 02 Jun 2019 23:18:48: 15000000 INFO @ Sun, 02 Jun 2019 23:18:51: 17000000 INFO @ Sun, 02 Jun 2019 23:18:54: 15000000 INFO @ Sun, 02 Jun 2019 23:18:58: 16000000 INFO @ Sun, 02 Jun 2019 23:18:59: 18000000 INFO @ Sun, 02 Jun 2019 23:19:04: 16000000 INFO @ Sun, 02 Jun 2019 23:19:08: 17000000 INFO @ Sun, 02 Jun 2019 23:19:08: 19000000 INFO @ Sun, 02 Jun 2019 23:19:14: 17000000 INFO @ Sun, 02 Jun 2019 23:19:17: 20000000 INFO @ Sun, 02 Jun 2019 23:19:18: 18000000 INFO @ Sun, 02 Jun 2019 23:19:24: 18000000 INFO @ Sun, 02 Jun 2019 23:19:26: 21000000 INFO @ Sun, 02 Jun 2019 23:19:28: 19000000 INFO @ Sun, 02 Jun 2019 23:19:34: 19000000 INFO @ Sun, 02 Jun 2019 23:19:35: 22000000 INFO @ Sun, 02 Jun 2019 23:19:37: #1 tag size is determined as 76 bps INFO @ Sun, 02 Jun 2019 23:19:37: #1 tag size = 76 INFO @ Sun, 02 Jun 2019 23:19:37: #1 total tags in treatment: 22219315 INFO @ Sun, 02 Jun 2019 23:19:37: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:19:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:19:38: #1 tags after filtering in treatment: 22219315 INFO @ Sun, 02 Jun 2019 23:19:38: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:19:38: #1 finished! INFO @ Sun, 02 Jun 2019 23:19:38: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:19:38: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:19:38: 20000000 INFO @ Sun, 02 Jun 2019 23:19:40: #2 number of paired peaks: 7 WARNING @ Sun, 02 Jun 2019 23:19:40: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:19:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:19:44: 20000000 INFO @ Sun, 02 Jun 2019 23:19:48: 21000000 INFO @ Sun, 02 Jun 2019 23:19:54: 21000000 INFO @ Sun, 02 Jun 2019 23:19:58: 22000000 INFO @ Sun, 02 Jun 2019 23:20:01: #1 tag size is determined as 76 bps INFO @ Sun, 02 Jun 2019 23:20:01: #1 tag size = 76 INFO @ Sun, 02 Jun 2019 23:20:01: #1 total tags in treatment: 22219315 INFO @ Sun, 02 Jun 2019 23:20:01: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:20:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:20:01: #1 tags after filtering in treatment: 22219315 INFO @ Sun, 02 Jun 2019 23:20:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:20:01: #1 finished! INFO @ Sun, 02 Jun 2019 23:20:01: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:20:01: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:20:03: #2 number of paired peaks: 7 WARNING @ Sun, 02 Jun 2019 23:20:03: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:20:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:20:04: 22000000 INFO @ Sun, 02 Jun 2019 23:20:06: #1 tag size is determined as 76 bps INFO @ Sun, 02 Jun 2019 23:20:06: #1 tag size = 76 INFO @ Sun, 02 Jun 2019 23:20:06: #1 total tags in treatment: 22219315 INFO @ Sun, 02 Jun 2019 23:20:06: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:20:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:20:07: #1 tags after filtering in treatment: 22219315 INFO @ Sun, 02 Jun 2019 23:20:07: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:20:07: #1 finished! INFO @ Sun, 02 Jun 2019 23:20:07: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:20:07: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:20:09: #2 number of paired peaks: 7 WARNING @ Sun, 02 Jun 2019 23:20:09: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:20:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX102376/ERX102376.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。