Job ID = 1293311 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 12,727,549 reads read : 12,727,549 reads written : 12,727,549 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:31 12727549 reads; of these: 12727549 (100.00%) were unpaired; of these: 887612 (6.97%) aligned 0 times 10873028 (85.43%) aligned exactly 1 time 966909 (7.60%) aligned >1 times 93.03% overall alignment rate Time searching: 00:02:31 Overall time: 00:02:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1630789 / 11839937 = 0.1377 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 22:59:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:59:30: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:59:30: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:59:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:59:30: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:59:30: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:59:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:59:30: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:59:30: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:59:37: 1000000 INFO @ Sun, 02 Jun 2019 22:59:38: 1000000 INFO @ Sun, 02 Jun 2019 22:59:38: 1000000 INFO @ Sun, 02 Jun 2019 22:59:44: 2000000 INFO @ Sun, 02 Jun 2019 22:59:46: 2000000 INFO @ Sun, 02 Jun 2019 22:59:47: 2000000 INFO @ Sun, 02 Jun 2019 22:59:51: 3000000 INFO @ Sun, 02 Jun 2019 22:59:53: 3000000 INFO @ Sun, 02 Jun 2019 22:59:55: 3000000 INFO @ Sun, 02 Jun 2019 22:59:59: 4000000 INFO @ Sun, 02 Jun 2019 23:00:01: 4000000 INFO @ Sun, 02 Jun 2019 23:00:03: 4000000 INFO @ Sun, 02 Jun 2019 23:00:06: 5000000 INFO @ Sun, 02 Jun 2019 23:00:09: 5000000 INFO @ Sun, 02 Jun 2019 23:00:11: 5000000 INFO @ Sun, 02 Jun 2019 23:00:13: 6000000 INFO @ Sun, 02 Jun 2019 23:00:16: 6000000 INFO @ Sun, 02 Jun 2019 23:00:19: 6000000 INFO @ Sun, 02 Jun 2019 23:00:20: 7000000 INFO @ Sun, 02 Jun 2019 23:00:24: 7000000 INFO @ Sun, 02 Jun 2019 23:00:27: 8000000 INFO @ Sun, 02 Jun 2019 23:00:27: 7000000 INFO @ Sun, 02 Jun 2019 23:00:31: 8000000 INFO @ Sun, 02 Jun 2019 23:00:34: 9000000 INFO @ Sun, 02 Jun 2019 23:00:36: 8000000 INFO @ Sun, 02 Jun 2019 23:00:39: 9000000 INFO @ Sun, 02 Jun 2019 23:00:42: 10000000 INFO @ Sun, 02 Jun 2019 23:00:44: 9000000 INFO @ Sun, 02 Jun 2019 23:00:44: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 23:00:44: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 23:00:44: #1 total tags in treatment: 10209148 INFO @ Sun, 02 Jun 2019 23:00:44: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:00:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:00:44: #1 tags after filtering in treatment: 10209148 INFO @ Sun, 02 Jun 2019 23:00:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:00:44: #1 finished! INFO @ Sun, 02 Jun 2019 23:00:44: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:00:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:00:45: #2 number of paired peaks: 9 WARNING @ Sun, 02 Jun 2019 23:00:45: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:00:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:00:47: 10000000 INFO @ Sun, 02 Jun 2019 23:00:48: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 23:00:48: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 23:00:48: #1 total tags in treatment: 10209148 INFO @ Sun, 02 Jun 2019 23:00:48: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:00:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:00:48: #1 tags after filtering in treatment: 10209148 INFO @ Sun, 02 Jun 2019 23:00:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:00:48: #1 finished! INFO @ Sun, 02 Jun 2019 23:00:48: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:00:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:00:49: #2 number of paired peaks: 9 WARNING @ Sun, 02 Jun 2019 23:00:49: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:00:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:00:52: 10000000 INFO @ Sun, 02 Jun 2019 23:00:53: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 23:00:53: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 23:00:53: #1 total tags in treatment: 10209148 INFO @ Sun, 02 Jun 2019 23:00:53: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:00:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:00:54: #1 tags after filtering in treatment: 10209148 INFO @ Sun, 02 Jun 2019 23:00:54: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:00:54: #1 finished! INFO @ Sun, 02 Jun 2019 23:00:54: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:00:54: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:00:54: #2 number of paired peaks: 9 WARNING @ Sun, 02 Jun 2019 23:00:54: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:00:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX101810/ERX101810.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。