Job ID = 6626450 SRX = ERX088875 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 87784639 spots for ERR111435/ERR111435.sra Written 87784639 spots for ERR111435/ERR111435.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626709 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:33:35 87784639 reads; of these: 87784639 (100.00%) were unpaired; of these: 11993917 (13.66%) aligned 0 times 55118819 (62.79%) aligned exactly 1 time 20671903 (23.55%) aligned >1 times 86.34% overall alignment rate Time searching: 00:33:35 Overall time: 00:33:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 37502413 / 75790722 = 0.4948 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:00:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:00:29: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:00:29: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:00:36: 1000000 INFO @ Tue, 14 Jul 2020 08:00:43: 2000000 INFO @ Tue, 14 Jul 2020 08:00:50: 3000000 INFO @ Tue, 14 Jul 2020 08:00:57: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:00:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:00:59: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:00:59: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:01:05: 5000000 INFO @ Tue, 14 Jul 2020 08:01:06: 1000000 INFO @ Tue, 14 Jul 2020 08:01:12: 6000000 INFO @ Tue, 14 Jul 2020 08:01:14: 2000000 INFO @ Tue, 14 Jul 2020 08:01:20: 7000000 INFO @ Tue, 14 Jul 2020 08:01:21: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:01:28: 8000000 INFO @ Tue, 14 Jul 2020 08:01:28: 4000000 INFO @ Tue, 14 Jul 2020 08:01:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:01:29: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:01:29: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:01:35: 9000000 INFO @ Tue, 14 Jul 2020 08:01:36: 5000000 INFO @ Tue, 14 Jul 2020 08:01:37: 1000000 INFO @ Tue, 14 Jul 2020 08:01:43: 6000000 INFO @ Tue, 14 Jul 2020 08:01:43: 10000000 INFO @ Tue, 14 Jul 2020 08:01:45: 2000000 INFO @ Tue, 14 Jul 2020 08:01:50: 7000000 INFO @ Tue, 14 Jul 2020 08:01:51: 11000000 INFO @ Tue, 14 Jul 2020 08:01:53: 3000000 INFO @ Tue, 14 Jul 2020 08:01:58: 8000000 INFO @ Tue, 14 Jul 2020 08:01:58: 12000000 INFO @ Tue, 14 Jul 2020 08:02:01: 4000000 INFO @ Tue, 14 Jul 2020 08:02:05: 9000000 INFO @ Tue, 14 Jul 2020 08:02:06: 13000000 INFO @ Tue, 14 Jul 2020 08:02:09: 5000000 INFO @ Tue, 14 Jul 2020 08:02:12: 10000000 INFO @ Tue, 14 Jul 2020 08:02:14: 14000000 INFO @ Tue, 14 Jul 2020 08:02:17: 6000000 INFO @ Tue, 14 Jul 2020 08:02:19: 11000000 INFO @ Tue, 14 Jul 2020 08:02:21: 15000000 INFO @ Tue, 14 Jul 2020 08:02:25: 7000000 INFO @ Tue, 14 Jul 2020 08:02:26: 12000000 INFO @ Tue, 14 Jul 2020 08:02:29: 16000000 INFO @ Tue, 14 Jul 2020 08:02:33: 13000000 INFO @ Tue, 14 Jul 2020 08:02:33: 8000000 INFO @ Tue, 14 Jul 2020 08:02:37: 17000000 INFO @ Tue, 14 Jul 2020 08:02:40: 14000000 INFO @ Tue, 14 Jul 2020 08:02:41: 9000000 INFO @ Tue, 14 Jul 2020 08:02:44: 18000000 INFO @ Tue, 14 Jul 2020 08:02:48: 15000000 INFO @ Tue, 14 Jul 2020 08:02:49: 10000000 INFO @ Tue, 14 Jul 2020 08:02:52: 19000000 INFO @ Tue, 14 Jul 2020 08:02:55: 16000000 INFO @ Tue, 14 Jul 2020 08:02:57: 11000000 INFO @ Tue, 14 Jul 2020 08:03:00: 20000000 INFO @ Tue, 14 Jul 2020 08:03:02: 17000000 INFO @ Tue, 14 Jul 2020 08:03:05: 12000000 INFO @ Tue, 14 Jul 2020 08:03:07: 21000000 INFO @ Tue, 14 Jul 2020 08:03:09: 18000000 INFO @ Tue, 14 Jul 2020 08:03:13: 13000000 INFO @ Tue, 14 Jul 2020 08:03:15: 22000000 INFO @ Tue, 14 Jul 2020 08:03:16: 19000000 INFO @ Tue, 14 Jul 2020 08:03:20: 14000000 INFO @ Tue, 14 Jul 2020 08:03:22: 23000000 INFO @ Tue, 14 Jul 2020 08:03:23: 20000000 INFO @ Tue, 14 Jul 2020 08:03:29: 15000000 INFO @ Tue, 14 Jul 2020 08:03:30: 24000000 INFO @ Tue, 14 Jul 2020 08:03:31: 21000000 INFO @ Tue, 14 Jul 2020 08:03:37: 16000000 INFO @ Tue, 14 Jul 2020 08:03:38: 22000000 INFO @ Tue, 14 Jul 2020 08:03:38: 25000000 INFO @ Tue, 14 Jul 2020 08:03:45: 23000000 INFO @ Tue, 14 Jul 2020 08:03:45: 17000000 INFO @ Tue, 14 Jul 2020 08:03:45: 26000000 INFO @ Tue, 14 Jul 2020 08:03:52: 24000000 INFO @ Tue, 14 Jul 2020 08:03:53: 27000000 INFO @ Tue, 14 Jul 2020 08:03:53: 18000000 INFO @ Tue, 14 Jul 2020 08:03:59: 25000000 INFO @ Tue, 14 Jul 2020 08:04:00: 28000000 INFO @ Tue, 14 Jul 2020 08:04:01: 19000000 INFO @ Tue, 14 Jul 2020 08:04:06: 26000000 INFO @ Tue, 14 Jul 2020 08:04:08: 29000000 INFO @ Tue, 14 Jul 2020 08:04:09: 20000000 INFO @ Tue, 14 Jul 2020 08:04:13: 27000000 INFO @ Tue, 14 Jul 2020 08:04:16: 30000000 INFO @ Tue, 14 Jul 2020 08:04:17: 21000000 INFO @ Tue, 14 Jul 2020 08:04:20: 28000000 INFO @ Tue, 14 Jul 2020 08:04:23: 31000000 INFO @ Tue, 14 Jul 2020 08:04:24: 22000000 INFO @ Tue, 14 Jul 2020 08:04:27: 29000000 INFO @ Tue, 14 Jul 2020 08:04:31: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:04:32: 23000000 INFO @ Tue, 14 Jul 2020 08:04:34: 30000000 INFO @ Tue, 14 Jul 2020 08:04:39: 33000000 INFO @ Tue, 14 Jul 2020 08:04:40: 24000000 INFO @ Tue, 14 Jul 2020 08:04:42: 31000000 INFO @ Tue, 14 Jul 2020 08:04:47: 34000000 INFO @ Tue, 14 Jul 2020 08:04:48: 25000000 INFO @ Tue, 14 Jul 2020 08:04:49: 32000000 INFO @ Tue, 14 Jul 2020 08:04:55: 35000000 INFO @ Tue, 14 Jul 2020 08:04:55: 26000000 INFO @ Tue, 14 Jul 2020 08:04:56: 33000000 INFO @ Tue, 14 Jul 2020 08:05:03: 36000000 INFO @ Tue, 14 Jul 2020 08:05:03: 27000000 INFO @ Tue, 14 Jul 2020 08:05:04: 34000000 INFO @ Tue, 14 Jul 2020 08:05:11: 37000000 INFO @ Tue, 14 Jul 2020 08:05:11: 28000000 INFO @ Tue, 14 Jul 2020 08:05:11: 35000000 INFO @ Tue, 14 Jul 2020 08:05:18: 36000000 INFO @ Tue, 14 Jul 2020 08:05:19: 38000000 INFO @ Tue, 14 Jul 2020 08:05:19: 29000000 INFO @ Tue, 14 Jul 2020 08:05:21: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:05:21: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:05:21: #1 total tags in treatment: 38288309 INFO @ Tue, 14 Jul 2020 08:05:21: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:05:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:05:22: #1 tags after filtering in treatment: 38288309 INFO @ Tue, 14 Jul 2020 08:05:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:05:22: #1 finished! INFO @ Tue, 14 Jul 2020 08:05:22: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:05:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:05:24: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:05:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:05:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:05:25: 37000000 INFO @ Tue, 14 Jul 2020 08:05:27: 30000000 INFO @ Tue, 14 Jul 2020 08:05:32: 38000000 INFO @ Tue, 14 Jul 2020 08:05:34: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:05:34: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:05:34: #1 total tags in treatment: 38288309 INFO @ Tue, 14 Jul 2020 08:05:34: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:05:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:05:34: 31000000 INFO @ Tue, 14 Jul 2020 08:05:35: #1 tags after filtering in treatment: 38288309 INFO @ Tue, 14 Jul 2020 08:05:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:05:35: #1 finished! INFO @ Tue, 14 Jul 2020 08:05:35: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:05:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:05:37: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:05:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:05:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:05:42: 32000000 INFO @ Tue, 14 Jul 2020 08:05:49: 33000000 INFO @ Tue, 14 Jul 2020 08:05:56: 34000000 INFO @ Tue, 14 Jul 2020 08:06:03: 35000000 INFO @ Tue, 14 Jul 2020 08:06:10: 36000000 INFO @ Tue, 14 Jul 2020 08:06:17: 37000000 INFO @ Tue, 14 Jul 2020 08:06:23: 38000000 INFO @ Tue, 14 Jul 2020 08:06:25: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:06:25: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:06:25: #1 total tags in treatment: 38288309 INFO @ Tue, 14 Jul 2020 08:06:25: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:06:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:06:26: #1 tags after filtering in treatment: 38288309 INFO @ Tue, 14 Jul 2020 08:06:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:06:26: #1 finished! INFO @ Tue, 14 Jul 2020 08:06:26: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:06:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:06:28: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:06:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:06:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088875/ERX088875.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling