Job ID = 6626443 SRX = ERX088871 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 88936078 spots for ERR111436/ERR111436.sra Written 88936078 spots for ERR111436/ERR111436.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626701 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:33:34 88936078 reads; of these: 88936078 (100.00%) were unpaired; of these: 10770230 (12.11%) aligned 0 times 57147706 (64.26%) aligned exactly 1 time 21018142 (23.63%) aligned >1 times 87.89% overall alignment rate Time searching: 00:33:34 Overall time: 00:33:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 32179458 / 78165848 = 0.4117 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:58:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:58:38: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:58:38: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:58:43: 1000000 INFO @ Tue, 14 Jul 2020 07:58:48: 2000000 INFO @ Tue, 14 Jul 2020 07:58:53: 3000000 INFO @ Tue, 14 Jul 2020 07:58:58: 4000000 INFO @ Tue, 14 Jul 2020 07:59:03: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:59:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:59:08: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:59:08: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:59:08: 6000000 INFO @ Tue, 14 Jul 2020 07:59:13: 1000000 INFO @ Tue, 14 Jul 2020 07:59:14: 7000000 INFO @ Tue, 14 Jul 2020 07:59:19: 2000000 INFO @ Tue, 14 Jul 2020 07:59:19: 8000000 INFO @ Tue, 14 Jul 2020 07:59:24: 3000000 INFO @ Tue, 14 Jul 2020 07:59:24: 9000000 INFO @ Tue, 14 Jul 2020 07:59:29: 4000000 INFO @ Tue, 14 Jul 2020 07:59:30: 10000000 INFO @ Tue, 14 Jul 2020 07:59:35: 5000000 INFO @ Tue, 14 Jul 2020 07:59:35: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:59:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:59:38: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:59:38: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:59:40: 6000000 INFO @ Tue, 14 Jul 2020 07:59:40: 12000000 INFO @ Tue, 14 Jul 2020 07:59:43: 1000000 INFO @ Tue, 14 Jul 2020 07:59:45: 7000000 INFO @ Tue, 14 Jul 2020 07:59:46: 13000000 INFO @ Tue, 14 Jul 2020 07:59:49: 2000000 INFO @ Tue, 14 Jul 2020 07:59:51: 8000000 INFO @ Tue, 14 Jul 2020 07:59:51: 14000000 INFO @ Tue, 14 Jul 2020 07:59:54: 3000000 INFO @ Tue, 14 Jul 2020 07:59:56: 9000000 INFO @ Tue, 14 Jul 2020 07:59:57: 15000000 INFO @ Tue, 14 Jul 2020 08:00:00: 4000000 INFO @ Tue, 14 Jul 2020 08:00:01: 10000000 INFO @ Tue, 14 Jul 2020 08:00:02: 16000000 INFO @ Tue, 14 Jul 2020 08:00:05: 5000000 INFO @ Tue, 14 Jul 2020 08:00:07: 11000000 INFO @ Tue, 14 Jul 2020 08:00:07: 17000000 INFO @ Tue, 14 Jul 2020 08:00:11: 6000000 INFO @ Tue, 14 Jul 2020 08:00:12: 12000000 INFO @ Tue, 14 Jul 2020 08:00:13: 18000000 INFO @ Tue, 14 Jul 2020 08:00:16: 7000000 INFO @ Tue, 14 Jul 2020 08:00:17: 13000000 INFO @ Tue, 14 Jul 2020 08:00:18: 19000000 INFO @ Tue, 14 Jul 2020 08:00:22: 8000000 INFO @ Tue, 14 Jul 2020 08:00:23: 14000000 INFO @ Tue, 14 Jul 2020 08:00:24: 20000000 INFO @ Tue, 14 Jul 2020 08:00:27: 9000000 INFO @ Tue, 14 Jul 2020 08:00:28: 15000000 INFO @ Tue, 14 Jul 2020 08:00:29: 21000000 INFO @ Tue, 14 Jul 2020 08:00:32: 10000000 INFO @ Tue, 14 Jul 2020 08:00:34: 16000000 INFO @ Tue, 14 Jul 2020 08:00:35: 22000000 INFO @ Tue, 14 Jul 2020 08:00:38: 11000000 INFO @ Tue, 14 Jul 2020 08:00:39: 17000000 INFO @ Tue, 14 Jul 2020 08:00:41: 23000000 INFO @ Tue, 14 Jul 2020 08:00:43: 12000000 INFO @ Tue, 14 Jul 2020 08:00:44: 18000000 INFO @ Tue, 14 Jul 2020 08:00:46: 24000000 INFO @ Tue, 14 Jul 2020 08:00:49: 13000000 INFO @ Tue, 14 Jul 2020 08:00:50: 19000000 INFO @ Tue, 14 Jul 2020 08:00:52: 25000000 INFO @ Tue, 14 Jul 2020 08:00:54: 14000000 INFO @ Tue, 14 Jul 2020 08:00:55: 20000000 INFO @ Tue, 14 Jul 2020 08:00:57: 26000000 INFO @ Tue, 14 Jul 2020 08:01:00: 15000000 INFO @ Tue, 14 Jul 2020 08:01:01: 21000000 INFO @ Tue, 14 Jul 2020 08:01:03: 27000000 INFO @ Tue, 14 Jul 2020 08:01:05: 16000000 INFO @ Tue, 14 Jul 2020 08:01:06: 22000000 INFO @ Tue, 14 Jul 2020 08:01:08: 28000000 INFO @ Tue, 14 Jul 2020 08:01:11: 17000000 INFO @ Tue, 14 Jul 2020 08:01:12: 23000000 INFO @ Tue, 14 Jul 2020 08:01:13: 29000000 INFO @ Tue, 14 Jul 2020 08:01:16: 18000000 INFO @ Tue, 14 Jul 2020 08:01:17: 24000000 INFO @ Tue, 14 Jul 2020 08:01:19: 30000000 INFO @ Tue, 14 Jul 2020 08:01:21: 19000000 INFO @ Tue, 14 Jul 2020 08:01:22: 25000000 INFO @ Tue, 14 Jul 2020 08:01:24: 31000000 INFO @ Tue, 14 Jul 2020 08:01:27: 20000000 INFO @ Tue, 14 Jul 2020 08:01:28: 26000000 INFO @ Tue, 14 Jul 2020 08:01:30: 32000000 INFO @ Tue, 14 Jul 2020 08:01:32: 21000000 INFO @ Tue, 14 Jul 2020 08:01:33: 27000000 INFO @ Tue, 14 Jul 2020 08:01:35: 33000000 INFO @ Tue, 14 Jul 2020 08:01:38: 22000000 INFO @ Tue, 14 Jul 2020 08:01:39: 28000000 INFO @ Tue, 14 Jul 2020 08:01:41: 34000000 INFO @ Tue, 14 Jul 2020 08:01:43: 23000000 INFO @ Tue, 14 Jul 2020 08:01:44: 29000000 INFO @ Tue, 14 Jul 2020 08:01:46: 35000000 INFO @ Tue, 14 Jul 2020 08:01:49: 24000000 INFO @ Tue, 14 Jul 2020 08:01:50: 30000000 INFO @ Tue, 14 Jul 2020 08:01:52: 36000000 INFO @ Tue, 14 Jul 2020 08:01:54: 25000000 INFO @ Tue, 14 Jul 2020 08:01:56: 31000000 INFO @ Tue, 14 Jul 2020 08:01:57: 37000000 INFO @ Tue, 14 Jul 2020 08:02:00: 26000000 INFO @ Tue, 14 Jul 2020 08:02:01: 32000000 INFO @ Tue, 14 Jul 2020 08:02:03: 38000000 INFO @ Tue, 14 Jul 2020 08:02:06: 27000000 INFO @ Tue, 14 Jul 2020 08:02:07: 33000000 INFO @ Tue, 14 Jul 2020 08:02:09: 39000000 INFO @ Tue, 14 Jul 2020 08:02:12: 28000000 INFO @ Tue, 14 Jul 2020 08:02:12: 34000000 INFO @ Tue, 14 Jul 2020 08:02:14: 40000000 INFO @ Tue, 14 Jul 2020 08:02:17: 29000000 INFO @ Tue, 14 Jul 2020 08:02:18: 35000000 INFO @ Tue, 14 Jul 2020 08:02:20: 41000000 INFO @ Tue, 14 Jul 2020 08:02:23: 30000000 INFO @ Tue, 14 Jul 2020 08:02:24: 36000000 INFO @ Tue, 14 Jul 2020 08:02:26: 42000000 INFO @ Tue, 14 Jul 2020 08:02:28: 31000000 INFO @ Tue, 14 Jul 2020 08:02:29: 37000000 INFO @ Tue, 14 Jul 2020 08:02:31: 43000000 INFO @ Tue, 14 Jul 2020 08:02:34: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:02:35: 38000000 INFO @ Tue, 14 Jul 2020 08:02:37: 44000000 INFO @ Tue, 14 Jul 2020 08:02:39: 33000000 INFO @ Tue, 14 Jul 2020 08:02:41: 39000000 INFO @ Tue, 14 Jul 2020 08:02:43: 45000000 INFO @ Tue, 14 Jul 2020 08:02:45: 34000000 INFO @ Tue, 14 Jul 2020 08:02:46: 40000000 INFO @ Tue, 14 Jul 2020 08:02:48: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:02:48: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:02:48: #1 total tags in treatment: 45986390 INFO @ Tue, 14 Jul 2020 08:02:48: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:02:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:02:49: #1 tags after filtering in treatment: 45986390 INFO @ Tue, 14 Jul 2020 08:02:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:02:49: #1 finished! INFO @ Tue, 14 Jul 2020 08:02:49: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:02:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:02:50: 35000000 INFO @ Tue, 14 Jul 2020 08:02:52: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:02:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:02:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:02:52: 41000000 INFO @ Tue, 14 Jul 2020 08:02:56: 36000000 INFO @ Tue, 14 Jul 2020 08:02:58: 42000000 INFO @ Tue, 14 Jul 2020 08:03:01: 37000000 INFO @ Tue, 14 Jul 2020 08:03:04: 43000000 INFO @ Tue, 14 Jul 2020 08:03:07: 38000000 INFO @ Tue, 14 Jul 2020 08:03:09: 44000000 INFO @ Tue, 14 Jul 2020 08:03:12: 39000000 INFO @ Tue, 14 Jul 2020 08:03:15: 45000000 INFO @ Tue, 14 Jul 2020 08:03:18: 40000000 INFO @ Tue, 14 Jul 2020 08:03:20: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:03:20: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:03:20: #1 total tags in treatment: 45986390 INFO @ Tue, 14 Jul 2020 08:03:20: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:03:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:03:21: #1 tags after filtering in treatment: 45986390 INFO @ Tue, 14 Jul 2020 08:03:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:03:21: #1 finished! INFO @ Tue, 14 Jul 2020 08:03:21: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:03:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:03:23: 41000000 INFO @ Tue, 14 Jul 2020 08:03:24: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:03:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:03:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:03:29: 42000000 INFO @ Tue, 14 Jul 2020 08:03:34: 43000000 INFO @ Tue, 14 Jul 2020 08:03:39: 44000000 INFO @ Tue, 14 Jul 2020 08:03:45: 45000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:03:50: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:03:50: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:03:50: #1 total tags in treatment: 45986390 INFO @ Tue, 14 Jul 2020 08:03:50: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:03:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:03:51: #1 tags after filtering in treatment: 45986390 INFO @ Tue, 14 Jul 2020 08:03:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:03:51: #1 finished! INFO @ Tue, 14 Jul 2020 08:03:51: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:03:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:03:54: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:03:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:03:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088871/ERX088871.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling