Job ID = 6626436 SRX = ERX088868 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 166371392 spots for ERR111440/ERR111440.sra Written 166371392 spots for ERR111440/ERR111440.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626729 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:42:46 166371392 reads; of these: 166371392 (100.00%) were unpaired; of these: 6106234 (3.67%) aligned 0 times 131629045 (79.12%) aligned exactly 1 time 28636113 (17.21%) aligned >1 times 96.33% overall alignment rate Time searching: 00:42:46 Overall time: 00:42:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 68 files... [bam_rmdupse_core] 101521331 / 160265158 = 0.6335 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:22:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:22:11: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:22:11: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:22:16: 1000000 INFO @ Tue, 14 Jul 2020 08:22:21: 2000000 INFO @ Tue, 14 Jul 2020 08:22:26: 3000000 INFO @ Tue, 14 Jul 2020 08:22:31: 4000000 INFO @ Tue, 14 Jul 2020 08:22:35: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:22:40: 6000000 INFO @ Tue, 14 Jul 2020 08:22:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:22:41: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:22:41: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:22:45: 7000000 INFO @ Tue, 14 Jul 2020 08:22:46: 1000000 INFO @ Tue, 14 Jul 2020 08:22:50: 8000000 INFO @ Tue, 14 Jul 2020 08:22:51: 2000000 INFO @ Tue, 14 Jul 2020 08:22:55: 9000000 INFO @ Tue, 14 Jul 2020 08:22:56: 3000000 INFO @ Tue, 14 Jul 2020 08:23:00: 10000000 INFO @ Tue, 14 Jul 2020 08:23:01: 4000000 INFO @ Tue, 14 Jul 2020 08:23:06: 11000000 INFO @ Tue, 14 Jul 2020 08:23:06: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:23:11: 12000000 INFO @ Tue, 14 Jul 2020 08:23:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:23:11: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:23:11: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:23:12: 6000000 INFO @ Tue, 14 Jul 2020 08:23:16: 13000000 INFO @ Tue, 14 Jul 2020 08:23:17: 1000000 INFO @ Tue, 14 Jul 2020 08:23:19: 7000000 INFO @ Tue, 14 Jul 2020 08:23:21: 14000000 INFO @ Tue, 14 Jul 2020 08:23:22: 2000000 INFO @ Tue, 14 Jul 2020 08:23:25: 8000000 INFO @ Tue, 14 Jul 2020 08:23:26: 15000000 INFO @ Tue, 14 Jul 2020 08:23:28: 3000000 INFO @ Tue, 14 Jul 2020 08:23:31: 16000000 INFO @ Tue, 14 Jul 2020 08:23:32: 9000000 INFO @ Tue, 14 Jul 2020 08:23:34: 4000000 INFO @ Tue, 14 Jul 2020 08:23:37: 17000000 INFO @ Tue, 14 Jul 2020 08:23:38: 10000000 INFO @ Tue, 14 Jul 2020 08:23:40: 5000000 INFO @ Tue, 14 Jul 2020 08:23:42: 18000000 INFO @ Tue, 14 Jul 2020 08:23:45: 11000000 INFO @ Tue, 14 Jul 2020 08:23:45: 6000000 INFO @ Tue, 14 Jul 2020 08:23:47: 19000000 INFO @ Tue, 14 Jul 2020 08:23:51: 7000000 INFO @ Tue, 14 Jul 2020 08:23:51: 12000000 INFO @ Tue, 14 Jul 2020 08:23:52: 20000000 INFO @ Tue, 14 Jul 2020 08:23:57: 8000000 INFO @ Tue, 14 Jul 2020 08:23:57: 21000000 INFO @ Tue, 14 Jul 2020 08:23:58: 13000000 INFO @ Tue, 14 Jul 2020 08:24:02: 9000000 INFO @ Tue, 14 Jul 2020 08:24:02: 22000000 INFO @ Tue, 14 Jul 2020 08:24:05: 14000000 INFO @ Tue, 14 Jul 2020 08:24:08: 23000000 INFO @ Tue, 14 Jul 2020 08:24:08: 10000000 INFO @ Tue, 14 Jul 2020 08:24:11: 15000000 INFO @ Tue, 14 Jul 2020 08:24:13: 24000000 INFO @ Tue, 14 Jul 2020 08:24:14: 11000000 INFO @ Tue, 14 Jul 2020 08:24:18: 16000000 INFO @ Tue, 14 Jul 2020 08:24:18: 25000000 INFO @ Tue, 14 Jul 2020 08:24:20: 12000000 INFO @ Tue, 14 Jul 2020 08:24:23: 26000000 INFO @ Tue, 14 Jul 2020 08:24:24: 17000000 INFO @ Tue, 14 Jul 2020 08:24:25: 13000000 INFO @ Tue, 14 Jul 2020 08:24:28: 27000000 INFO @ Tue, 14 Jul 2020 08:24:31: 18000000 INFO @ Tue, 14 Jul 2020 08:24:31: 14000000 INFO @ Tue, 14 Jul 2020 08:24:33: 28000000 INFO @ Tue, 14 Jul 2020 08:24:37: 15000000 INFO @ Tue, 14 Jul 2020 08:24:37: 19000000 INFO @ Tue, 14 Jul 2020 08:24:39: 29000000 INFO @ Tue, 14 Jul 2020 08:24:43: 16000000 INFO @ Tue, 14 Jul 2020 08:24:44: 30000000 INFO @ Tue, 14 Jul 2020 08:24:44: 20000000 INFO @ Tue, 14 Jul 2020 08:24:48: 17000000 INFO @ Tue, 14 Jul 2020 08:24:49: 31000000 INFO @ Tue, 14 Jul 2020 08:24:50: 21000000 INFO @ Tue, 14 Jul 2020 08:24:54: 32000000 INFO @ Tue, 14 Jul 2020 08:24:54: 18000000 INFO @ Tue, 14 Jul 2020 08:24:57: 22000000 INFO @ Tue, 14 Jul 2020 08:24:59: 33000000 INFO @ Tue, 14 Jul 2020 08:25:00: 19000000 INFO @ Tue, 14 Jul 2020 08:25:03: 23000000 INFO @ Tue, 14 Jul 2020 08:25:04: 34000000 INFO @ Tue, 14 Jul 2020 08:25:06: 20000000 INFO @ Tue, 14 Jul 2020 08:25:09: 24000000 INFO @ Tue, 14 Jul 2020 08:25:10: 35000000 INFO @ Tue, 14 Jul 2020 08:25:12: 21000000 INFO @ Tue, 14 Jul 2020 08:25:15: 36000000 INFO @ Tue, 14 Jul 2020 08:25:15: 25000000 INFO @ Tue, 14 Jul 2020 08:25:17: 22000000 INFO @ Tue, 14 Jul 2020 08:25:20: 37000000 INFO @ Tue, 14 Jul 2020 08:25:22: 26000000 INFO @ Tue, 14 Jul 2020 08:25:23: 23000000 INFO @ Tue, 14 Jul 2020 08:25:25: 38000000 INFO @ Tue, 14 Jul 2020 08:25:28: 27000000 INFO @ Tue, 14 Jul 2020 08:25:29: 24000000 INFO @ Tue, 14 Jul 2020 08:25:30: 39000000 INFO @ Tue, 14 Jul 2020 08:25:35: 28000000 INFO @ Tue, 14 Jul 2020 08:25:35: 25000000 INFO @ Tue, 14 Jul 2020 08:25:35: 40000000 INFO @ Tue, 14 Jul 2020 08:25:40: 26000000 INFO @ Tue, 14 Jul 2020 08:25:41: 41000000 INFO @ Tue, 14 Jul 2020 08:25:41: 29000000 INFO @ Tue, 14 Jul 2020 08:25:46: 42000000 INFO @ Tue, 14 Jul 2020 08:25:46: 27000000 INFO @ Tue, 14 Jul 2020 08:25:47: 30000000 INFO @ Tue, 14 Jul 2020 08:25:51: 43000000 INFO @ Tue, 14 Jul 2020 08:25:52: 28000000 INFO @ Tue, 14 Jul 2020 08:25:53: 31000000 INFO @ Tue, 14 Jul 2020 08:25:56: 44000000 INFO @ Tue, 14 Jul 2020 08:25:58: 29000000 INFO @ Tue, 14 Jul 2020 08:25:59: 32000000 INFO @ Tue, 14 Jul 2020 08:26:01: 45000000 INFO @ Tue, 14 Jul 2020 08:26:04: 30000000 INFO @ Tue, 14 Jul 2020 08:26:05: 33000000 INFO @ Tue, 14 Jul 2020 08:26:06: 46000000 INFO @ Tue, 14 Jul 2020 08:26:09: 31000000 INFO @ Tue, 14 Jul 2020 08:26:12: 34000000 INFO @ Tue, 14 Jul 2020 08:26:12: 47000000 INFO @ Tue, 14 Jul 2020 08:26:15: 32000000 INFO @ Tue, 14 Jul 2020 08:26:17: 48000000 INFO @ Tue, 14 Jul 2020 08:26:18: 35000000 INFO @ Tue, 14 Jul 2020 08:26:21: 33000000 INFO @ Tue, 14 Jul 2020 08:26:22: 49000000 INFO @ Tue, 14 Jul 2020 08:26:24: 36000000 INFO @ Tue, 14 Jul 2020 08:26:27: 34000000 INFO @ Tue, 14 Jul 2020 08:26:27: 50000000 INFO @ Tue, 14 Jul 2020 08:26:31: 37000000 INFO @ Tue, 14 Jul 2020 08:26:32: 35000000 INFO @ Tue, 14 Jul 2020 08:26:32: 51000000 INFO @ Tue, 14 Jul 2020 08:26:37: 38000000 INFO @ Tue, 14 Jul 2020 08:26:37: 52000000 INFO @ Tue, 14 Jul 2020 08:26:38: 36000000 INFO @ Tue, 14 Jul 2020 08:26:42: 53000000 INFO @ Tue, 14 Jul 2020 08:26:44: 37000000 INFO @ Tue, 14 Jul 2020 08:26:44: 39000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:26:48: 54000000 INFO @ Tue, 14 Jul 2020 08:26:49: 38000000 INFO @ Tue, 14 Jul 2020 08:26:50: 40000000 INFO @ Tue, 14 Jul 2020 08:26:53: 55000000 INFO @ Tue, 14 Jul 2020 08:26:55: 39000000 INFO @ Tue, 14 Jul 2020 08:26:57: 41000000 INFO @ Tue, 14 Jul 2020 08:26:58: 56000000 INFO @ Tue, 14 Jul 2020 08:27:01: 40000000 INFO @ Tue, 14 Jul 2020 08:27:03: 57000000 INFO @ Tue, 14 Jul 2020 08:27:03: 42000000 INFO @ Tue, 14 Jul 2020 08:27:06: 41000000 INFO @ Tue, 14 Jul 2020 08:27:08: 58000000 INFO @ Tue, 14 Jul 2020 08:27:10: 43000000 INFO @ Tue, 14 Jul 2020 08:27:12: 42000000 INFO @ Tue, 14 Jul 2020 08:27:12: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:27:12: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:27:12: #1 total tags in treatment: 58743827 INFO @ Tue, 14 Jul 2020 08:27:12: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:27:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:27:13: #1 tags after filtering in treatment: 58743827 INFO @ Tue, 14 Jul 2020 08:27:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:27:13: #1 finished! INFO @ Tue, 14 Jul 2020 08:27:13: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:27:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:27:16: 44000000 INFO @ Tue, 14 Jul 2020 08:27:17: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:27:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:27:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:27:18: 43000000 INFO @ Tue, 14 Jul 2020 08:27:23: 45000000 INFO @ Tue, 14 Jul 2020 08:27:24: 44000000 INFO @ Tue, 14 Jul 2020 08:27:29: 45000000 INFO @ Tue, 14 Jul 2020 08:27:30: 46000000 INFO @ Tue, 14 Jul 2020 08:27:35: 46000000 INFO @ Tue, 14 Jul 2020 08:27:36: 47000000 INFO @ Tue, 14 Jul 2020 08:27:41: 47000000 INFO @ Tue, 14 Jul 2020 08:27:43: 48000000 INFO @ Tue, 14 Jul 2020 08:27:46: 48000000 INFO @ Tue, 14 Jul 2020 08:27:49: 49000000 INFO @ Tue, 14 Jul 2020 08:27:52: 49000000 INFO @ Tue, 14 Jul 2020 08:27:56: 50000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:27:58: 50000000 INFO @ Tue, 14 Jul 2020 08:28:02: 51000000 INFO @ Tue, 14 Jul 2020 08:28:03: 51000000 INFO @ Tue, 14 Jul 2020 08:28:08: 52000000 INFO @ Tue, 14 Jul 2020 08:28:09: 52000000 INFO @ Tue, 14 Jul 2020 08:28:15: 53000000 INFO @ Tue, 14 Jul 2020 08:28:15: 53000000 INFO @ Tue, 14 Jul 2020 08:28:21: 54000000 INFO @ Tue, 14 Jul 2020 08:28:22: 54000000 INFO @ Tue, 14 Jul 2020 08:28:26: 55000000 INFO @ Tue, 14 Jul 2020 08:28:28: 55000000 INFO @ Tue, 14 Jul 2020 08:28:32: 56000000 INFO @ Tue, 14 Jul 2020 08:28:35: 56000000 INFO @ Tue, 14 Jul 2020 08:28:38: 57000000 INFO @ Tue, 14 Jul 2020 08:28:42: 57000000 INFO @ Tue, 14 Jul 2020 08:28:43: 58000000 INFO @ Tue, 14 Jul 2020 08:28:48: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:28:48: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:28:48: #1 total tags in treatment: 58743827 INFO @ Tue, 14 Jul 2020 08:28:48: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:28:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:28:48: 58000000 INFO @ Tue, 14 Jul 2020 08:28:49: #1 tags after filtering in treatment: 58743827 INFO @ Tue, 14 Jul 2020 08:28:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:28:49: #1 finished! INFO @ Tue, 14 Jul 2020 08:28:49: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:28:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:28:52: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:28:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:28:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:28:53: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:28:53: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:28:53: #1 total tags in treatment: 58743827 INFO @ Tue, 14 Jul 2020 08:28:53: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:28:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:28:54: #1 tags after filtering in treatment: 58743827 INFO @ Tue, 14 Jul 2020 08:28:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:28:54: #1 finished! INFO @ Tue, 14 Jul 2020 08:28:54: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:28:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:28:58: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:28:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:28:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX088868/ERX088868.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling