Job ID = 1293255 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 2,710,538 reads read : 2,710,538 reads written : 2,710,538 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:31 2710538 reads; of these: 2710538 (100.00%) were unpaired; of these: 5083 (0.19%) aligned 0 times 2433947 (89.80%) aligned exactly 1 time 271508 (10.02%) aligned >1 times 99.81% overall alignment rate Time searching: 00:00:32 Overall time: 00:00:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 86985 / 2705455 = 0.0322 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 22:32:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:32:32: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:32:32: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:32:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:32:32: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:32:32: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:32:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:32:32: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:32:32: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:32:41: 1000000 INFO @ Sun, 02 Jun 2019 22:32:41: 1000000 INFO @ Sun, 02 Jun 2019 22:32:42: 1000000 INFO @ Sun, 02 Jun 2019 22:32:49: 2000000 INFO @ Sun, 02 Jun 2019 22:32:50: 2000000 INFO @ Sun, 02 Jun 2019 22:32:52: 2000000 INFO @ Sun, 02 Jun 2019 22:32:54: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 22:32:54: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 22:32:54: #1 total tags in treatment: 2618470 INFO @ Sun, 02 Jun 2019 22:32:54: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:32:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:32:54: #1 tags after filtering in treatment: 2618470 INFO @ Sun, 02 Jun 2019 22:32:54: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 22:32:54: #1 finished! INFO @ Sun, 02 Jun 2019 22:32:54: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:32:54: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:32:54: #2 number of paired peaks: 32 WARNING @ Sun, 02 Jun 2019 22:32:54: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 22:32:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 22:32:55: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 22:32:55: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 22:32:55: #1 total tags in treatment: 2618470 INFO @ Sun, 02 Jun 2019 22:32:55: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:32:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:32:55: #1 tags after filtering in treatment: 2618470 INFO @ Sun, 02 Jun 2019 22:32:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 22:32:55: #1 finished! INFO @ Sun, 02 Jun 2019 22:32:55: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:32:55: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:32:55: #2 number of paired peaks: 32 WARNING @ Sun, 02 Jun 2019 22:32:55: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 22:32:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 22:32:58: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 22:32:58: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 22:32:58: #1 total tags in treatment: 2618470 INFO @ Sun, 02 Jun 2019 22:32:58: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:32:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:32:58: #1 tags after filtering in treatment: 2618470 INFO @ Sun, 02 Jun 2019 22:32:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 22:32:58: #1 finished! INFO @ Sun, 02 Jun 2019 22:32:58: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:32:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:32:59: #2 number of paired peaks: 32 WARNING @ Sun, 02 Jun 2019 22:32:59: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 22:32:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX008178/ERX008178.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。