SRX065714 SAMN00620892 GSM729371:_Snyder_DAF-12_GFP_L4_rep2_extraction4_seq4_aliquote_1 ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 2439120 Illumina_Genome_Analyzer SRA037225 SRS209125 SRP006868 PRJNA141631 2013-09-23T16:45:14Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_DAF-12_GFP_L4_rep2 extraction4_seq4 channel_1 strain=OP222(official name : OP222 genotype : unc119(ed3); wgIs222(daf-12:TY1 EGFP FLAG; unc119) outcross : 3 transgene : daf-12 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The DAF-12::EGFP fusion protein is expressed in the correct daf-12 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the DAF-12 transcription factor. made_by : Mihail Sarov ) developmental stage=L4 genotype=unc119(ed3);wgIs222(daf-12:TY1 EGFP FLAG;unc119) Sex=hermaphrodite transgene=daf-12