SRX065705	SAMN00620883	GSM729374:_Snyder_DAF-12_GFP_L3_rep1_extraction3_seq3_aliquote_1	ChIP-Seq	GENOMIC	ChIP	0	Application_Read	Forward	6455317	Illumina_Genome_Analyzer	SRA037223	SRS209116	SRP006866	PRJNA141633	2013-09-23T16:45:14Z	Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans"	source_name=Snyder_DAF-12_GFP_L3_rep1 extraction3_seq3 channel_1	strain=OP222(official name : OP222 genotype : unc119(ed3); wgIs222(daf-12:TY1 EGFP FLAG; unc119) outcross : 3 transgene : daf-12 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The DAF-12::EGFP fusion protein is expressed in the correct daf-12 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the DAF-12 transcription factor. made_by : Mihail Sarov )	developmental stage=L3	genotype=unc119(ed3);wgIs222(daf-12:TY1 EGFP FLAG;unc119)	Sex=hermaphrodite	transgene=daf-12