SRX054250 SAMN00254081 GSM700165:_Snyder_ALR-1_GFP_L2_rep1_extraction3_seq3_aliquote_1 ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 5712711 Illumina_Genome_Analyzer SRA030936 SRS183337 SRP006198 PRJNA63461 2013-09-23T16:45:14Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_ALR-1_GFP_L2_rep1 extraction3_seq3 channel_1 strain=OP200(official name : OP200 genotype : unc119(ed3); wgIs200(alr-1::TY1 EGFP FLAG C; unc119) outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : his strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The ALR-1::EGFP fusion protein is expressed in the correct alr-1 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the ALR-1 transcription factor. made_by : R. Waterston ) developmental stage=L2 genotype=unc119(ed3);wgIs200(alr-1::TY1 EGFP FLAG C;unc119) Sex=hermaphrodite