SRX044010 SAMN00216275 GSM677690:_Snyder_EGL-27_GFP_L1_rep2_extraction2_seq1_aliquote_2 ChIP-Seq GENOMIC ChIP 0 Application_Read Forward 5285403 Illumina_Genome_Analyzer SRA030394 SRS173197 SRP005887 PRJNA63461 2013-09-23T16:45:14Z Organism taxonomy_id="6239" taxonomy_name="Caenorhabditis elegans" source_name=Snyder_EGL-27_GFP_L1_rep2 extraction2_seq1 channel_2 strain=OP177(made_by : S Kim description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The EGL-27::EGFP fusion protein is expressed in the correct egl-27 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the EGL-27 transcription factor. tags : GFP::3xFlag mutagen : Bombard outcross : 3 genotype : unc119(ed3); wgIs177(egl-27::TY1 EGFP FLAG; unc119) official name : OP177 ) developmental stage=fed L1 genotype=unc119(ed3);wgIs177(egl-27::TY1 EGFP FLAG;unc119) Sex=hermaphrodite