Job ID = 6369046 SRX = SRX982110 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:44:12 prefetch.2.10.7: 1) Downloading 'SRR1956600'... 2020-06-16T00:44:12 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:48:42 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:48:42 prefetch.2.10.7: 1) 'SRR1956600' was downloaded successfully Read 39842908 spots for SRR1956600/SRR1956600.sra Written 39842908 spots for SRR1956600/SRR1956600.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:25 39842908 reads; of these: 39842908 (100.00%) were unpaired; of these: 5598231 (14.05%) aligned 0 times 28790786 (72.26%) aligned exactly 1 time 5453891 (13.69%) aligned >1 times 85.95% overall alignment rate Time searching: 00:08:25 Overall time: 00:08:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5411799 / 34244677 = 0.1580 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:08:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:08:56: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:08:56: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:09:01: 1000000 INFO @ Tue, 16 Jun 2020 10:09:06: 2000000 INFO @ Tue, 16 Jun 2020 10:09:11: 3000000 INFO @ Tue, 16 Jun 2020 10:09:16: 4000000 INFO @ Tue, 16 Jun 2020 10:09:20: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:09:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:09:25: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:09:25: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:09:26: 6000000 INFO @ Tue, 16 Jun 2020 10:09:32: 1000000 INFO @ Tue, 16 Jun 2020 10:09:32: 7000000 INFO @ Tue, 16 Jun 2020 10:09:38: 2000000 INFO @ Tue, 16 Jun 2020 10:09:38: 8000000 INFO @ Tue, 16 Jun 2020 10:09:44: 3000000 INFO @ Tue, 16 Jun 2020 10:09:44: 9000000 INFO @ Tue, 16 Jun 2020 10:09:50: 4000000 INFO @ Tue, 16 Jun 2020 10:09:51: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:09:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:09:55: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:09:55: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:09:56: 5000000 INFO @ Tue, 16 Jun 2020 10:09:57: 11000000 INFO @ Tue, 16 Jun 2020 10:10:00: 1000000 INFO @ Tue, 16 Jun 2020 10:10:03: 6000000 INFO @ Tue, 16 Jun 2020 10:10:03: 12000000 INFO @ Tue, 16 Jun 2020 10:10:05: 2000000 INFO @ Tue, 16 Jun 2020 10:10:10: 7000000 INFO @ Tue, 16 Jun 2020 10:10:10: 3000000 INFO @ Tue, 16 Jun 2020 10:10:10: 13000000 INFO @ Tue, 16 Jun 2020 10:10:15: 4000000 INFO @ Tue, 16 Jun 2020 10:10:16: 8000000 INFO @ Tue, 16 Jun 2020 10:10:17: 14000000 INFO @ Tue, 16 Jun 2020 10:10:20: 5000000 INFO @ Tue, 16 Jun 2020 10:10:23: 9000000 INFO @ Tue, 16 Jun 2020 10:10:24: 15000000 INFO @ Tue, 16 Jun 2020 10:10:26: 6000000 INFO @ Tue, 16 Jun 2020 10:10:30: 10000000 INFO @ Tue, 16 Jun 2020 10:10:30: 7000000 INFO @ Tue, 16 Jun 2020 10:10:31: 16000000 INFO @ Tue, 16 Jun 2020 10:10:36: 8000000 INFO @ Tue, 16 Jun 2020 10:10:36: 11000000 INFO @ Tue, 16 Jun 2020 10:10:38: 17000000 INFO @ Tue, 16 Jun 2020 10:10:41: 9000000 INFO @ Tue, 16 Jun 2020 10:10:43: 12000000 INFO @ Tue, 16 Jun 2020 10:10:44: 18000000 INFO @ Tue, 16 Jun 2020 10:10:45: 10000000 INFO @ Tue, 16 Jun 2020 10:10:50: 13000000 INFO @ Tue, 16 Jun 2020 10:10:51: 11000000 INFO @ Tue, 16 Jun 2020 10:10:51: 19000000 INFO @ Tue, 16 Jun 2020 10:10:55: 12000000 INFO @ Tue, 16 Jun 2020 10:10:57: 14000000 INFO @ Tue, 16 Jun 2020 10:10:58: 20000000 INFO @ Tue, 16 Jun 2020 10:11:01: 13000000 INFO @ Tue, 16 Jun 2020 10:11:03: 15000000 INFO @ Tue, 16 Jun 2020 10:11:04: 21000000 INFO @ Tue, 16 Jun 2020 10:11:06: 14000000 INFO @ Tue, 16 Jun 2020 10:11:10: 16000000 INFO @ Tue, 16 Jun 2020 10:11:11: 15000000 INFO @ Tue, 16 Jun 2020 10:11:11: 22000000 INFO @ Tue, 16 Jun 2020 10:11:16: 16000000 INFO @ Tue, 16 Jun 2020 10:11:16: 17000000 INFO @ Tue, 16 Jun 2020 10:11:17: 23000000 INFO @ Tue, 16 Jun 2020 10:11:21: 17000000 INFO @ Tue, 16 Jun 2020 10:11:22: 18000000 INFO @ Tue, 16 Jun 2020 10:11:24: 24000000 INFO @ Tue, 16 Jun 2020 10:11:27: 18000000 INFO @ Tue, 16 Jun 2020 10:11:29: 19000000 INFO @ Tue, 16 Jun 2020 10:11:30: 25000000 INFO @ Tue, 16 Jun 2020 10:11:32: 19000000 INFO @ Tue, 16 Jun 2020 10:11:35: 20000000 INFO @ Tue, 16 Jun 2020 10:11:36: 26000000 INFO @ Tue, 16 Jun 2020 10:11:37: 20000000 INFO @ Tue, 16 Jun 2020 10:11:41: 21000000 INFO @ Tue, 16 Jun 2020 10:11:42: 21000000 INFO @ Tue, 16 Jun 2020 10:11:42: 27000000 INFO @ Tue, 16 Jun 2020 10:11:46: 22000000 INFO @ Tue, 16 Jun 2020 10:11:47: 22000000 INFO @ Tue, 16 Jun 2020 10:11:48: 28000000 INFO @ Tue, 16 Jun 2020 10:11:52: 23000000 INFO @ Tue, 16 Jun 2020 10:11:52: 23000000 INFO @ Tue, 16 Jun 2020 10:11:53: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:11:53: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:11:53: #1 total tags in treatment: 28832878 INFO @ Tue, 16 Jun 2020 10:11:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:11:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:11:53: #1 tags after filtering in treatment: 28832878 INFO @ Tue, 16 Jun 2020 10:11:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:11:53: #1 finished! INFO @ Tue, 16 Jun 2020 10:11:53: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:11:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:11:55: #2 number of paired peaks: 86 WARNING @ Tue, 16 Jun 2020 10:11:55: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:11:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:11:57: 24000000 INFO @ Tue, 16 Jun 2020 10:11:57: 24000000 INFO @ Tue, 16 Jun 2020 10:12:03: 25000000 INFO @ Tue, 16 Jun 2020 10:12:03: 25000000 INFO @ Tue, 16 Jun 2020 10:12:08: 26000000 INFO @ Tue, 16 Jun 2020 10:12:08: 26000000 INFO @ Tue, 16 Jun 2020 10:12:13: 27000000 INFO @ Tue, 16 Jun 2020 10:12:13: 27000000 INFO @ Tue, 16 Jun 2020 10:12:18: 28000000 INFO @ Tue, 16 Jun 2020 10:12:19: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:12:23: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:12:23: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:12:23: #1 total tags in treatment: 28832878 INFO @ Tue, 16 Jun 2020 10:12:23: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:12:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:12:23: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:12:23: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:12:23: #1 total tags in treatment: 28832878 INFO @ Tue, 16 Jun 2020 10:12:23: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:12:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:12:23: #1 tags after filtering in treatment: 28832878 INFO @ Tue, 16 Jun 2020 10:12:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:12:23: #1 finished! INFO @ Tue, 16 Jun 2020 10:12:23: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:12:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:12:23: #1 tags after filtering in treatment: 28832878 INFO @ Tue, 16 Jun 2020 10:12:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:12:23: #1 finished! INFO @ Tue, 16 Jun 2020 10:12:23: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:12:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:12:25: #2 number of paired peaks: 86 WARNING @ Tue, 16 Jun 2020 10:12:25: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:12:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:12:25: #2 number of paired peaks: 86 WARNING @ Tue, 16 Jun 2020 10:12:25: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:12:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX982110/SRX982110.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。