Job ID = 6368993 SRX = SRX958298 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:42:43 prefetch.2.10.7: 1) Downloading 'SRR1917671'... 2020-06-16T00:42:43 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:50:53 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:50:53 prefetch.2.10.7: 1) 'SRR1917671' was downloaded successfully Read 64271618 spots for SRR1917671/SRR1917671.sra Written 64271618 spots for SRR1917671/SRR1917671.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:09 64271618 reads; of these: 64271618 (100.00%) were unpaired; of these: 20793658 (32.35%) aligned 0 times 35328635 (54.97%) aligned exactly 1 time 8149325 (12.68%) aligned >1 times 67.65% overall alignment rate Time searching: 00:12:09 Overall time: 00:12:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 11100723 / 43477960 = 0.2553 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:17:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:17:44: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:17:44: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:17:50: 1000000 INFO @ Tue, 16 Jun 2020 10:17:57: 2000000 INFO @ Tue, 16 Jun 2020 10:18:03: 3000000 INFO @ Tue, 16 Jun 2020 10:18:09: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:18:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:18:14: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:18:14: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:18:15: 5000000 INFO @ Tue, 16 Jun 2020 10:18:21: 1000000 INFO @ Tue, 16 Jun 2020 10:18:21: 6000000 INFO @ Tue, 16 Jun 2020 10:18:27: 2000000 INFO @ Tue, 16 Jun 2020 10:18:28: 7000000 INFO @ Tue, 16 Jun 2020 10:18:33: 3000000 INFO @ Tue, 16 Jun 2020 10:18:34: 8000000 INFO @ Tue, 16 Jun 2020 10:18:39: 4000000 INFO @ Tue, 16 Jun 2020 10:18:40: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:18:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:18:44: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:18:44: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:18:45: 5000000 INFO @ Tue, 16 Jun 2020 10:18:47: 10000000 INFO @ Tue, 16 Jun 2020 10:18:51: 1000000 INFO @ Tue, 16 Jun 2020 10:18:51: 6000000 INFO @ Tue, 16 Jun 2020 10:18:53: 11000000 INFO @ Tue, 16 Jun 2020 10:18:57: 2000000 INFO @ Tue, 16 Jun 2020 10:18:58: 7000000 INFO @ Tue, 16 Jun 2020 10:18:59: 12000000 INFO @ Tue, 16 Jun 2020 10:19:03: 3000000 INFO @ Tue, 16 Jun 2020 10:19:04: 8000000 INFO @ Tue, 16 Jun 2020 10:19:06: 13000000 INFO @ Tue, 16 Jun 2020 10:19:10: 4000000 INFO @ Tue, 16 Jun 2020 10:19:10: 9000000 INFO @ Tue, 16 Jun 2020 10:19:12: 14000000 INFO @ Tue, 16 Jun 2020 10:19:16: 5000000 INFO @ Tue, 16 Jun 2020 10:19:16: 10000000 INFO @ Tue, 16 Jun 2020 10:19:18: 15000000 INFO @ Tue, 16 Jun 2020 10:19:22: 11000000 INFO @ Tue, 16 Jun 2020 10:19:22: 6000000 INFO @ Tue, 16 Jun 2020 10:19:25: 16000000 INFO @ Tue, 16 Jun 2020 10:19:28: 12000000 INFO @ Tue, 16 Jun 2020 10:19:28: 7000000 INFO @ Tue, 16 Jun 2020 10:19:31: 17000000 INFO @ Tue, 16 Jun 2020 10:19:34: 13000000 INFO @ Tue, 16 Jun 2020 10:19:35: 8000000 INFO @ Tue, 16 Jun 2020 10:19:37: 18000000 INFO @ Tue, 16 Jun 2020 10:19:40: 14000000 INFO @ Tue, 16 Jun 2020 10:19:41: 9000000 INFO @ Tue, 16 Jun 2020 10:19:43: 19000000 INFO @ Tue, 16 Jun 2020 10:19:47: 15000000 INFO @ Tue, 16 Jun 2020 10:19:47: 10000000 INFO @ Tue, 16 Jun 2020 10:19:49: 20000000 INFO @ Tue, 16 Jun 2020 10:19:53: 16000000 INFO @ Tue, 16 Jun 2020 10:19:53: 11000000 INFO @ Tue, 16 Jun 2020 10:19:55: 21000000 INFO @ Tue, 16 Jun 2020 10:19:59: 17000000 INFO @ Tue, 16 Jun 2020 10:20:00: 12000000 INFO @ Tue, 16 Jun 2020 10:20:01: 22000000 INFO @ Tue, 16 Jun 2020 10:20:05: 18000000 INFO @ Tue, 16 Jun 2020 10:20:06: 13000000 INFO @ Tue, 16 Jun 2020 10:20:07: 23000000 INFO @ Tue, 16 Jun 2020 10:20:11: 19000000 INFO @ Tue, 16 Jun 2020 10:20:12: 14000000 INFO @ Tue, 16 Jun 2020 10:20:13: 24000000 INFO @ Tue, 16 Jun 2020 10:20:17: 20000000 INFO @ Tue, 16 Jun 2020 10:20:18: 15000000 INFO @ Tue, 16 Jun 2020 10:20:19: 25000000 INFO @ Tue, 16 Jun 2020 10:20:24: 21000000 INFO @ Tue, 16 Jun 2020 10:20:25: 16000000 INFO @ Tue, 16 Jun 2020 10:20:25: 26000000 INFO @ Tue, 16 Jun 2020 10:20:30: 22000000 INFO @ Tue, 16 Jun 2020 10:20:31: 17000000 INFO @ Tue, 16 Jun 2020 10:20:31: 27000000 INFO @ Tue, 16 Jun 2020 10:20:36: 23000000 INFO @ Tue, 16 Jun 2020 10:20:37: 18000000 INFO @ Tue, 16 Jun 2020 10:20:38: 28000000 INFO @ Tue, 16 Jun 2020 10:20:42: 24000000 INFO @ Tue, 16 Jun 2020 10:20:43: 19000000 INFO @ Tue, 16 Jun 2020 10:20:43: 29000000 INFO @ Tue, 16 Jun 2020 10:20:48: 25000000 INFO @ Tue, 16 Jun 2020 10:20:49: 20000000 INFO @ Tue, 16 Jun 2020 10:20:49: 30000000 INFO @ Tue, 16 Jun 2020 10:20:54: 26000000 INFO @ Tue, 16 Jun 2020 10:20:55: 21000000 INFO @ Tue, 16 Jun 2020 10:20:55: 31000000 INFO @ Tue, 16 Jun 2020 10:21:00: 27000000 INFO @ Tue, 16 Jun 2020 10:21:01: 22000000 INFO @ Tue, 16 Jun 2020 10:21:01: 32000000 INFO @ Tue, 16 Jun 2020 10:21:04: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:21:04: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:21:04: #1 total tags in treatment: 32377237 INFO @ Tue, 16 Jun 2020 10:21:04: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:21:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:21:05: #1 tags after filtering in treatment: 32377237 INFO @ Tue, 16 Jun 2020 10:21:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:21:05: #1 finished! INFO @ Tue, 16 Jun 2020 10:21:05: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:21:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:21:06: 28000000 INFO @ Tue, 16 Jun 2020 10:21:07: #2 number of paired peaks: 55 WARNING @ Tue, 16 Jun 2020 10:21:07: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:21:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:21:08: 23000000 INFO @ Tue, 16 Jun 2020 10:21:12: 29000000 INFO @ Tue, 16 Jun 2020 10:21:14: 24000000 INFO @ Tue, 16 Jun 2020 10:21:18: 30000000 INFO @ Tue, 16 Jun 2020 10:21:20: 25000000 INFO @ Tue, 16 Jun 2020 10:21:24: 31000000 INFO @ Tue, 16 Jun 2020 10:21:26: 26000000 INFO @ Tue, 16 Jun 2020 10:21:30: 32000000 INFO @ Tue, 16 Jun 2020 10:21:32: 27000000 INFO @ Tue, 16 Jun 2020 10:21:33: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:21:33: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:21:33: #1 total tags in treatment: 32377237 INFO @ Tue, 16 Jun 2020 10:21:33: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:21:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:21:33: #1 tags after filtering in treatment: 32377237 INFO @ Tue, 16 Jun 2020 10:21:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:21:33: #1 finished! INFO @ Tue, 16 Jun 2020 10:21:33: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:21:33: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:21:35: #2 number of paired peaks: 55 WARNING @ Tue, 16 Jun 2020 10:21:35: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:21:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:21:38: 28000000 INFO @ Tue, 16 Jun 2020 10:21:43: 29000000 INFO @ Tue, 16 Jun 2020 10:21:49: 30000000 INFO @ Tue, 16 Jun 2020 10:21:55: 31000000 INFO @ Tue, 16 Jun 2020 10:22:01: 32000000 INFO @ Tue, 16 Jun 2020 10:22:03: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:22:03: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:22:03: #1 total tags in treatment: 32377237 INFO @ Tue, 16 Jun 2020 10:22:03: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:22:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:22:04: #1 tags after filtering in treatment: 32377237 INFO @ Tue, 16 Jun 2020 10:22:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:22:04: #1 finished! INFO @ Tue, 16 Jun 2020 10:22:04: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:22:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:22:06: #2 number of paired peaks: 55 WARNING @ Tue, 16 Jun 2020 10:22:06: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:22:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX958298/SRX958298.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。