Job ID = 14160495 SRX = SRX9555453 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:12 39068285 reads; of these: 39068285 (100.00%) were paired; of these: 4282875 (10.96%) aligned concordantly 0 times 30096040 (77.03%) aligned concordantly exactly 1 time 4689370 (12.00%) aligned concordantly >1 times ---- 4282875 pairs aligned concordantly 0 times; of these: 2118434 (49.46%) aligned discordantly 1 time ---- 2164441 pairs aligned 0 times concordantly or discordantly; of these: 4328882 mates make up the pairs; of these: 2550436 (58.92%) aligned 0 times 1156403 (26.71%) aligned exactly 1 time 622043 (14.37%) aligned >1 times 96.74% overall alignment rate Time searching: 00:31:13 Overall time: 00:31:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 7240012 / 36880307 = 0.1963 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:36:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:36:25: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:36:25: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:36:31: 1000000 INFO @ Thu, 09 Dec 2021 03:36:36: 2000000 INFO @ Thu, 09 Dec 2021 03:36:41: 3000000 INFO @ Thu, 09 Dec 2021 03:36:46: 4000000 INFO @ Thu, 09 Dec 2021 03:36:52: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:36:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:36:55: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:36:55: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:36:57: 6000000 INFO @ Thu, 09 Dec 2021 03:37:03: 1000000 INFO @ Thu, 09 Dec 2021 03:37:04: 7000000 INFO @ Thu, 09 Dec 2021 03:37:11: 2000000 INFO @ Thu, 09 Dec 2021 03:37:11: 8000000 INFO @ Thu, 09 Dec 2021 03:37:17: 9000000 INFO @ Thu, 09 Dec 2021 03:37:18: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:37:24: 10000000 INFO @ Thu, 09 Dec 2021 03:37:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:37:25: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:37:25: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:37:26: 4000000 INFO @ Thu, 09 Dec 2021 03:37:31: 11000000 INFO @ Thu, 09 Dec 2021 03:37:33: 5000000 INFO @ Thu, 09 Dec 2021 03:37:33: 1000000 INFO @ Thu, 09 Dec 2021 03:37:38: 12000000 INFO @ Thu, 09 Dec 2021 03:37:41: 6000000 INFO @ Thu, 09 Dec 2021 03:37:41: 2000000 INFO @ Thu, 09 Dec 2021 03:37:45: 13000000 INFO @ Thu, 09 Dec 2021 03:37:48: 7000000 INFO @ Thu, 09 Dec 2021 03:37:49: 3000000 INFO @ Thu, 09 Dec 2021 03:37:52: 14000000 INFO @ Thu, 09 Dec 2021 03:37:56: 8000000 INFO @ Thu, 09 Dec 2021 03:37:57: 4000000 INFO @ Thu, 09 Dec 2021 03:37:59: 15000000 INFO @ Thu, 09 Dec 2021 03:38:04: 9000000 INFO @ Thu, 09 Dec 2021 03:38:05: 5000000 INFO @ Thu, 09 Dec 2021 03:38:06: 16000000 INFO @ Thu, 09 Dec 2021 03:38:11: 10000000 INFO @ Thu, 09 Dec 2021 03:38:13: 17000000 INFO @ Thu, 09 Dec 2021 03:38:13: 6000000 INFO @ Thu, 09 Dec 2021 03:38:19: 11000000 INFO @ Thu, 09 Dec 2021 03:38:20: 18000000 INFO @ Thu, 09 Dec 2021 03:38:21: 7000000 INFO @ Thu, 09 Dec 2021 03:38:26: 12000000 INFO @ Thu, 09 Dec 2021 03:38:27: 19000000 INFO @ Thu, 09 Dec 2021 03:38:29: 8000000 INFO @ Thu, 09 Dec 2021 03:38:34: 13000000 INFO @ Thu, 09 Dec 2021 03:38:34: 20000000 INFO @ Thu, 09 Dec 2021 03:38:37: 9000000 INFO @ Thu, 09 Dec 2021 03:38:41: 21000000 INFO @ Thu, 09 Dec 2021 03:38:41: 14000000 INFO @ Thu, 09 Dec 2021 03:38:45: 10000000 INFO @ Thu, 09 Dec 2021 03:38:47: 22000000 INFO @ Thu, 09 Dec 2021 03:38:49: 15000000 INFO @ Thu, 09 Dec 2021 03:38:53: 11000000 INFO @ Thu, 09 Dec 2021 03:38:54: 23000000 INFO @ Thu, 09 Dec 2021 03:38:57: 16000000 INFO @ Thu, 09 Dec 2021 03:39:01: 12000000 INFO @ Thu, 09 Dec 2021 03:39:01: 24000000 INFO @ Thu, 09 Dec 2021 03:39:05: 17000000 INFO @ Thu, 09 Dec 2021 03:39:08: 25000000 INFO @ Thu, 09 Dec 2021 03:39:09: 13000000 INFO @ Thu, 09 Dec 2021 03:39:13: 18000000 INFO @ Thu, 09 Dec 2021 03:39:15: 26000000 INFO @ Thu, 09 Dec 2021 03:39:17: 14000000 INFO @ Thu, 09 Dec 2021 03:39:20: 19000000 INFO @ Thu, 09 Dec 2021 03:39:22: 27000000 INFO @ Thu, 09 Dec 2021 03:39:24: 15000000 INFO @ Thu, 09 Dec 2021 03:39:28: 20000000 INFO @ Thu, 09 Dec 2021 03:39:28: 28000000 INFO @ Thu, 09 Dec 2021 03:39:32: 16000000 INFO @ Thu, 09 Dec 2021 03:39:35: 29000000 INFO @ Thu, 09 Dec 2021 03:39:36: 21000000 INFO @ Thu, 09 Dec 2021 03:39:40: 17000000 INFO @ Thu, 09 Dec 2021 03:39:42: 30000000 INFO @ Thu, 09 Dec 2021 03:39:44: 22000000 INFO @ Thu, 09 Dec 2021 03:39:48: 18000000 INFO @ Thu, 09 Dec 2021 03:39:49: 31000000 INFO @ Thu, 09 Dec 2021 03:39:51: 23000000 INFO @ Thu, 09 Dec 2021 03:39:56: 32000000 INFO @ Thu, 09 Dec 2021 03:39:56: 19000000 INFO @ Thu, 09 Dec 2021 03:39:59: 24000000 INFO @ Thu, 09 Dec 2021 03:40:03: 33000000 INFO @ Thu, 09 Dec 2021 03:40:04: 20000000 INFO @ Thu, 09 Dec 2021 03:40:07: 25000000 INFO @ Thu, 09 Dec 2021 03:40:09: 34000000 INFO @ Thu, 09 Dec 2021 03:40:12: 21000000 INFO @ Thu, 09 Dec 2021 03:40:14: 26000000 INFO @ Thu, 09 Dec 2021 03:40:16: 35000000 INFO @ Thu, 09 Dec 2021 03:40:20: 22000000 INFO @ Thu, 09 Dec 2021 03:40:22: 27000000 INFO @ Thu, 09 Dec 2021 03:40:23: 36000000 INFO @ Thu, 09 Dec 2021 03:40:28: 23000000 INFO @ Thu, 09 Dec 2021 03:40:29: 28000000 INFO @ Thu, 09 Dec 2021 03:40:30: 37000000 INFO @ Thu, 09 Dec 2021 03:40:35: 24000000 INFO @ Thu, 09 Dec 2021 03:40:37: 29000000 INFO @ Thu, 09 Dec 2021 03:40:37: 38000000 INFO @ Thu, 09 Dec 2021 03:40:43: 25000000 INFO @ Thu, 09 Dec 2021 03:40:44: 39000000 INFO @ Thu, 09 Dec 2021 03:40:44: 30000000 INFO @ Thu, 09 Dec 2021 03:40:51: 40000000 INFO @ Thu, 09 Dec 2021 03:40:51: 26000000 INFO @ Thu, 09 Dec 2021 03:40:52: 31000000 INFO @ Thu, 09 Dec 2021 03:40:57: 41000000 INFO @ Thu, 09 Dec 2021 03:40:58: 27000000 INFO @ Thu, 09 Dec 2021 03:40:59: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 03:41:04: 42000000 INFO @ Thu, 09 Dec 2021 03:41:06: 28000000 INFO @ Thu, 09 Dec 2021 03:41:07: 33000000 INFO @ Thu, 09 Dec 2021 03:41:11: 43000000 INFO @ Thu, 09 Dec 2021 03:41:14: 29000000 INFO @ Thu, 09 Dec 2021 03:41:15: 34000000 INFO @ Thu, 09 Dec 2021 03:41:18: 44000000 INFO @ Thu, 09 Dec 2021 03:41:22: 30000000 INFO @ Thu, 09 Dec 2021 03:41:23: 35000000 INFO @ Thu, 09 Dec 2021 03:41:25: 45000000 INFO @ Thu, 09 Dec 2021 03:41:29: 31000000 INFO @ Thu, 09 Dec 2021 03:41:30: 36000000 INFO @ Thu, 09 Dec 2021 03:41:32: 46000000 INFO @ Thu, 09 Dec 2021 03:41:37: 32000000 INFO @ Thu, 09 Dec 2021 03:41:38: 37000000 INFO @ Thu, 09 Dec 2021 03:41:39: 47000000 INFO @ Thu, 09 Dec 2021 03:41:45: 33000000 INFO @ Thu, 09 Dec 2021 03:41:45: 48000000 INFO @ Thu, 09 Dec 2021 03:41:46: 38000000 INFO @ Thu, 09 Dec 2021 03:41:52: 49000000 INFO @ Thu, 09 Dec 2021 03:41:53: 34000000 INFO @ Thu, 09 Dec 2021 03:41:53: 39000000 INFO @ Thu, 09 Dec 2021 03:41:59: 50000000 INFO @ Thu, 09 Dec 2021 03:42:01: 35000000 INFO @ Thu, 09 Dec 2021 03:42:01: 40000000 INFO @ Thu, 09 Dec 2021 03:42:06: 51000000 INFO @ Thu, 09 Dec 2021 03:42:08: 36000000 INFO @ Thu, 09 Dec 2021 03:42:09: 41000000 INFO @ Thu, 09 Dec 2021 03:42:13: 52000000 INFO @ Thu, 09 Dec 2021 03:42:16: 37000000 INFO @ Thu, 09 Dec 2021 03:42:16: 42000000 INFO @ Thu, 09 Dec 2021 03:42:20: 53000000 BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 03:42:24: 38000000 INFO @ Thu, 09 Dec 2021 03:42:24: 43000000 INFO @ Thu, 09 Dec 2021 03:42:26: 54000000 INFO @ Thu, 09 Dec 2021 03:42:31: 39000000 INFO @ Thu, 09 Dec 2021 03:42:31: 44000000 INFO @ Thu, 09 Dec 2021 03:42:33: 55000000 INFO @ Thu, 09 Dec 2021 03:42:39: 45000000 INFO @ Thu, 09 Dec 2021 03:42:39: 40000000 INFO @ Thu, 09 Dec 2021 03:42:40: 56000000 INFO @ Thu, 09 Dec 2021 03:42:47: 46000000 INFO @ Thu, 09 Dec 2021 03:42:47: 57000000 INFO @ Thu, 09 Dec 2021 03:42:47: 41000000 INFO @ Thu, 09 Dec 2021 03:42:54: 58000000 INFO @ Thu, 09 Dec 2021 03:42:54: 47000000 INFO @ Thu, 09 Dec 2021 03:42:55: 42000000 INFO @ Thu, 09 Dec 2021 03:43:01: 59000000 INFO @ Thu, 09 Dec 2021 03:43:02: 48000000 INFO @ Thu, 09 Dec 2021 03:43:02: 43000000 INFO @ Thu, 09 Dec 2021 03:43:08: 60000000 INFO @ Thu, 09 Dec 2021 03:43:10: 49000000 INFO @ Thu, 09 Dec 2021 03:43:10: 44000000 INFO @ Thu, 09 Dec 2021 03:43:15: 61000000 INFO @ Thu, 09 Dec 2021 03:43:15: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 03:43:15: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 03:43:15: #1 total tags in treatment: 27775775 INFO @ Thu, 09 Dec 2021 03:43:15: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:43:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:43:16: #1 tags after filtering in treatment: 24821534 INFO @ Thu, 09 Dec 2021 03:43:16: #1 Redundant rate of treatment: 0.11 INFO @ Thu, 09 Dec 2021 03:43:16: #1 finished! INFO @ Thu, 09 Dec 2021 03:43:16: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:43:16: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:43:17: 50000000 INFO @ Thu, 09 Dec 2021 03:43:17: #2 number of paired peaks: 198 WARNING @ Thu, 09 Dec 2021 03:43:17: Fewer paired peaks (198) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 198 pairs to build model! INFO @ Thu, 09 Dec 2021 03:43:17: start model_add_line... INFO @ Thu, 09 Dec 2021 03:43:18: start X-correlation... INFO @ Thu, 09 Dec 2021 03:43:18: end of X-cor INFO @ Thu, 09 Dec 2021 03:43:18: #2 finished! INFO @ Thu, 09 Dec 2021 03:43:18: #2 predicted fragment length is 61 bps INFO @ Thu, 09 Dec 2021 03:43:18: #2 alternative fragment length(s) may be 2,61,162 bps INFO @ Thu, 09 Dec 2021 03:43:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.05_model.r WARNING @ Thu, 09 Dec 2021 03:43:18: #2 Since the d (61) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 03:43:18: #2 You may need to consider one of the other alternative d(s): 2,61,162 WARNING @ Thu, 09 Dec 2021 03:43:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 03:43:18: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:43:18: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:43:18: 45000000 INFO @ Thu, 09 Dec 2021 03:43:25: 51000000 INFO @ Thu, 09 Dec 2021 03:43:25: 46000000 INFO @ Thu, 09 Dec 2021 03:43:32: 52000000 INFO @ Thu, 09 Dec 2021 03:43:33: 47000000 INFO @ Thu, 09 Dec 2021 03:43:40: 53000000 INFO @ Thu, 09 Dec 2021 03:43:40: 48000000 INFO @ Thu, 09 Dec 2021 03:43:47: 54000000 INFO @ Thu, 09 Dec 2021 03:43:48: 49000000 INFO @ Thu, 09 Dec 2021 03:43:53: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:43:55: 55000000 INFO @ Thu, 09 Dec 2021 03:43:56: 50000000 INFO @ Thu, 09 Dec 2021 03:44:02: 56000000 INFO @ Thu, 09 Dec 2021 03:44:03: 51000000 INFO @ Thu, 09 Dec 2021 03:44:09: 57000000 INFO @ Thu, 09 Dec 2021 03:44:11: 52000000 INFO @ Thu, 09 Dec 2021 03:44:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.05_peaks.xls INFO @ Thu, 09 Dec 2021 03:44:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:44:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.05_summits.bed INFO @ Thu, 09 Dec 2021 03:44:11: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (681 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 03:44:17: 58000000 INFO @ Thu, 09 Dec 2021 03:44:18: 53000000 INFO @ Thu, 09 Dec 2021 03:44:24: 59000000 INFO @ Thu, 09 Dec 2021 03:44:26: 54000000 INFO @ Thu, 09 Dec 2021 03:44:32: 60000000 INFO @ Thu, 09 Dec 2021 03:44:34: 55000000 INFO @ Thu, 09 Dec 2021 03:44:39: 61000000 INFO @ Thu, 09 Dec 2021 03:44:40: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 03:44:40: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 03:44:40: #1 total tags in treatment: 27775775 INFO @ Thu, 09 Dec 2021 03:44:40: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:44:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:44:40: #1 tags after filtering in treatment: 24821534 INFO @ Thu, 09 Dec 2021 03:44:40: #1 Redundant rate of treatment: 0.11 INFO @ Thu, 09 Dec 2021 03:44:40: #1 finished! INFO @ Thu, 09 Dec 2021 03:44:40: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:44:40: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:44:41: 56000000 INFO @ Thu, 09 Dec 2021 03:44:42: #2 number of paired peaks: 198 WARNING @ Thu, 09 Dec 2021 03:44:42: Fewer paired peaks (198) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 198 pairs to build model! INFO @ Thu, 09 Dec 2021 03:44:42: start model_add_line... INFO @ Thu, 09 Dec 2021 03:44:42: start X-correlation... INFO @ Thu, 09 Dec 2021 03:44:42: end of X-cor INFO @ Thu, 09 Dec 2021 03:44:42: #2 finished! INFO @ Thu, 09 Dec 2021 03:44:42: #2 predicted fragment length is 61 bps INFO @ Thu, 09 Dec 2021 03:44:42: #2 alternative fragment length(s) may be 2,61,162 bps INFO @ Thu, 09 Dec 2021 03:44:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.10_model.r WARNING @ Thu, 09 Dec 2021 03:44:42: #2 Since the d (61) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 03:44:42: #2 You may need to consider one of the other alternative d(s): 2,61,162 WARNING @ Thu, 09 Dec 2021 03:44:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 03:44:42: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:44:42: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:44:48: 57000000 INFO @ Thu, 09 Dec 2021 03:44:55: 58000000 INFO @ Thu, 09 Dec 2021 03:45:02: 59000000 INFO @ Thu, 09 Dec 2021 03:45:09: 60000000 INFO @ Thu, 09 Dec 2021 03:45:16: 61000000 INFO @ Thu, 09 Dec 2021 03:45:17: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 03:45:17: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 03:45:17: #1 total tags in treatment: 27775775 INFO @ Thu, 09 Dec 2021 03:45:17: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:45:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:45:17: #1 tags after filtering in treatment: 24821534 INFO @ Thu, 09 Dec 2021 03:45:17: #1 Redundant rate of treatment: 0.11 INFO @ Thu, 09 Dec 2021 03:45:17: #1 finished! INFO @ Thu, 09 Dec 2021 03:45:17: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:45:17: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:45:19: #2 number of paired peaks: 198 WARNING @ Thu, 09 Dec 2021 03:45:19: Fewer paired peaks (198) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 198 pairs to build model! INFO @ Thu, 09 Dec 2021 03:45:19: start model_add_line... INFO @ Thu, 09 Dec 2021 03:45:19: start X-correlation... INFO @ Thu, 09 Dec 2021 03:45:19: end of X-cor INFO @ Thu, 09 Dec 2021 03:45:19: #2 finished! INFO @ Thu, 09 Dec 2021 03:45:19: #2 predicted fragment length is 61 bps INFO @ Thu, 09 Dec 2021 03:45:19: #2 alternative fragment length(s) may be 2,61,162 bps INFO @ Thu, 09 Dec 2021 03:45:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.20_model.r WARNING @ Thu, 09 Dec 2021 03:45:19: #2 Since the d (61) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 03:45:19: #2 You may need to consider one of the other alternative d(s): 2,61,162 WARNING @ Thu, 09 Dec 2021 03:45:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 03:45:19: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:45:19: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:45:20: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:45:37: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.10_peaks.xls INFO @ Thu, 09 Dec 2021 03:45:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:45:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.10_summits.bed INFO @ Thu, 09 Dec 2021 03:45:37: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (482 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 03:45:55: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:46:12: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.20_peaks.xls INFO @ Thu, 09 Dec 2021 03:46:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:46:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX9555453/SRX9555453.20_summits.bed INFO @ Thu, 09 Dec 2021 03:46:12: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (202 records, 4 fields): 1 millis CompletedMACS2peakCalling