Job ID = 10166130 SRX = SRX9120610 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:06 42619141 reads; of these: 42619141 (100.00%) were unpaired; of these: 4681503 (10.98%) aligned 0 times 31268672 (73.37%) aligned exactly 1 time 6668966 (15.65%) aligned >1 times 89.02% overall alignment rate Time searching: 00:08:06 Overall time: 00:08:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7557753 / 37937638 = 0.1992 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:47:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:47:00: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:47:00: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:47:06: 1000000 INFO @ Thu, 08 Oct 2020 20:47:11: 2000000 INFO @ Thu, 08 Oct 2020 20:47:16: 3000000 INFO @ Thu, 08 Oct 2020 20:47:21: 4000000 INFO @ Thu, 08 Oct 2020 20:47:26: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:47:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:47:30: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:47:30: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:47:31: 6000000 INFO @ Thu, 08 Oct 2020 20:47:36: 1000000 INFO @ Thu, 08 Oct 2020 20:47:36: 7000000 INFO @ Thu, 08 Oct 2020 20:47:41: 2000000 INFO @ Thu, 08 Oct 2020 20:47:41: 8000000 INFO @ Thu, 08 Oct 2020 20:47:47: 3000000 INFO @ Thu, 08 Oct 2020 20:47:47: 9000000 INFO @ Thu, 08 Oct 2020 20:47:52: 4000000 INFO @ Thu, 08 Oct 2020 20:47:52: 10000000 INFO @ Thu, 08 Oct 2020 20:47:58: 5000000 INFO @ Thu, 08 Oct 2020 20:47:58: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:48:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:48:00: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:48:00: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:48:03: 6000000 INFO @ Thu, 08 Oct 2020 20:48:03: 12000000 INFO @ Thu, 08 Oct 2020 20:48:06: 1000000 INFO @ Thu, 08 Oct 2020 20:48:09: 7000000 INFO @ Thu, 08 Oct 2020 20:48:09: 13000000 INFO @ Thu, 08 Oct 2020 20:48:11: 2000000 INFO @ Thu, 08 Oct 2020 20:48:14: 8000000 INFO @ Thu, 08 Oct 2020 20:48:15: 14000000 INFO @ Thu, 08 Oct 2020 20:48:17: 3000000 INFO @ Thu, 08 Oct 2020 20:48:20: 9000000 INFO @ Thu, 08 Oct 2020 20:48:20: 15000000 INFO @ Thu, 08 Oct 2020 20:48:23: 4000000 INFO @ Thu, 08 Oct 2020 20:48:26: 10000000 INFO @ Thu, 08 Oct 2020 20:48:26: 16000000 INFO @ Thu, 08 Oct 2020 20:48:28: 5000000 INFO @ Thu, 08 Oct 2020 20:48:31: 11000000 INFO @ Thu, 08 Oct 2020 20:48:32: 17000000 INFO @ Thu, 08 Oct 2020 20:48:34: 6000000 INFO @ Thu, 08 Oct 2020 20:48:37: 12000000 INFO @ Thu, 08 Oct 2020 20:48:37: 18000000 INFO @ Thu, 08 Oct 2020 20:48:40: 7000000 INFO @ Thu, 08 Oct 2020 20:48:43: 13000000 INFO @ Thu, 08 Oct 2020 20:48:43: 19000000 INFO @ Thu, 08 Oct 2020 20:48:45: 8000000 INFO @ Thu, 08 Oct 2020 20:48:48: 14000000 INFO @ Thu, 08 Oct 2020 20:48:48: 20000000 INFO @ Thu, 08 Oct 2020 20:48:51: 9000000 INFO @ Thu, 08 Oct 2020 20:48:54: 21000000 INFO @ Thu, 08 Oct 2020 20:48:54: 15000000 INFO @ Thu, 08 Oct 2020 20:48:57: 10000000 INFO @ Thu, 08 Oct 2020 20:49:00: 22000000 INFO @ Thu, 08 Oct 2020 20:49:00: 16000000 INFO @ Thu, 08 Oct 2020 20:49:02: 11000000 INFO @ Thu, 08 Oct 2020 20:49:05: 23000000 INFO @ Thu, 08 Oct 2020 20:49:05: 17000000 INFO @ Thu, 08 Oct 2020 20:49:08: 12000000 INFO @ Thu, 08 Oct 2020 20:49:11: 24000000 INFO @ Thu, 08 Oct 2020 20:49:11: 18000000 INFO @ Thu, 08 Oct 2020 20:49:13: 13000000 INFO @ Thu, 08 Oct 2020 20:49:16: 25000000 INFO @ Thu, 08 Oct 2020 20:49:16: 19000000 INFO @ Thu, 08 Oct 2020 20:49:19: 14000000 INFO @ Thu, 08 Oct 2020 20:49:22: 26000000 INFO @ Thu, 08 Oct 2020 20:49:22: 20000000 INFO @ Thu, 08 Oct 2020 20:49:25: 15000000 INFO @ Thu, 08 Oct 2020 20:49:27: 27000000 INFO @ Thu, 08 Oct 2020 20:49:28: 21000000 INFO @ Thu, 08 Oct 2020 20:49:30: 16000000 INFO @ Thu, 08 Oct 2020 20:49:33: 28000000 INFO @ Thu, 08 Oct 2020 20:49:33: 22000000 INFO @ Thu, 08 Oct 2020 20:49:36: 17000000 INFO @ Thu, 08 Oct 2020 20:49:38: 29000000 INFO @ Thu, 08 Oct 2020 20:49:39: 23000000 INFO @ Thu, 08 Oct 2020 20:49:42: 18000000 INFO @ Thu, 08 Oct 2020 20:49:44: 30000000 INFO @ Thu, 08 Oct 2020 20:49:44: 24000000 INFO @ Thu, 08 Oct 2020 20:49:46: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:49:46: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:49:46: #1 total tags in treatment: 30379885 INFO @ Thu, 08 Oct 2020 20:49:46: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:49:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:49:47: #1 tags after filtering in treatment: 30379885 INFO @ Thu, 08 Oct 2020 20:49:47: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:49:47: #1 finished! INFO @ Thu, 08 Oct 2020 20:49:47: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:49:47: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:49:47: 19000000 INFO @ Thu, 08 Oct 2020 20:49:48: #2 number of paired peaks: 98 WARNING @ Thu, 08 Oct 2020 20:49:48: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:49:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:49:50: 25000000 INFO @ Thu, 08 Oct 2020 20:49:53: 20000000 INFO @ Thu, 08 Oct 2020 20:49:55: 26000000 INFO @ Thu, 08 Oct 2020 20:49:58: 21000000 INFO @ Thu, 08 Oct 2020 20:50:01: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:50:04: 22000000 INFO @ Thu, 08 Oct 2020 20:50:06: 28000000 INFO @ Thu, 08 Oct 2020 20:50:09: 23000000 INFO @ Thu, 08 Oct 2020 20:50:12: 29000000 INFO @ Thu, 08 Oct 2020 20:50:15: 24000000 INFO @ Thu, 08 Oct 2020 20:50:17: 30000000 INFO @ Thu, 08 Oct 2020 20:50:19: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:50:19: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:50:19: #1 total tags in treatment: 30379885 INFO @ Thu, 08 Oct 2020 20:50:19: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:50:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:50:20: #1 tags after filtering in treatment: 30379885 INFO @ Thu, 08 Oct 2020 20:50:20: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:50:20: #1 finished! INFO @ Thu, 08 Oct 2020 20:50:20: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:50:20: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:50:20: 25000000 INFO @ Thu, 08 Oct 2020 20:50:22: #2 number of paired peaks: 98 WARNING @ Thu, 08 Oct 2020 20:50:22: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:50:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:50:26: 26000000 INFO @ Thu, 08 Oct 2020 20:50:31: 27000000 INFO @ Thu, 08 Oct 2020 20:50:36: 28000000 INFO @ Thu, 08 Oct 2020 20:50:41: 29000000 INFO @ Thu, 08 Oct 2020 20:50:46: 30000000 INFO @ Thu, 08 Oct 2020 20:50:48: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:50:48: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:50:48: #1 total tags in treatment: 30379885 INFO @ Thu, 08 Oct 2020 20:50:48: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:50:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:50:48: #1 tags after filtering in treatment: 30379885 INFO @ Thu, 08 Oct 2020 20:50:48: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:50:48: #1 finished! INFO @ Thu, 08 Oct 2020 20:50:48: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:50:48: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:50:50: #2 number of paired peaks: 98 WARNING @ Thu, 08 Oct 2020 20:50:50: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:50:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。