Job ID = 14159415 SRX = SRX9120610 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:40 42619141 reads; of these: 42619141 (100.00%) were unpaired; of these: 4681503 (10.98%) aligned 0 times 31268672 (73.37%) aligned exactly 1 time 6668966 (15.65%) aligned >1 times 89.02% overall alignment rate Time searching: 00:07:40 Overall time: 00:07:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7557753 / 37937638 = 0.1992 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 21:47:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 21:47:59: #1 read tag files... INFO @ Wed, 08 Dec 2021 21:47:59: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 21:48:04: 1000000 INFO @ Wed, 08 Dec 2021 21:48:09: 2000000 INFO @ Wed, 08 Dec 2021 21:48:14: 3000000 INFO @ Wed, 08 Dec 2021 21:48:19: 4000000 INFO @ Wed, 08 Dec 2021 21:48:25: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 21:48:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 21:48:28: #1 read tag files... INFO @ Wed, 08 Dec 2021 21:48:28: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 21:48:30: 6000000 INFO @ Wed, 08 Dec 2021 21:48:34: 1000000 INFO @ Wed, 08 Dec 2021 21:48:36: 7000000 INFO @ Wed, 08 Dec 2021 21:48:40: 2000000 INFO @ Wed, 08 Dec 2021 21:48:42: 8000000 INFO @ Wed, 08 Dec 2021 21:48:46: 3000000 INFO @ Wed, 08 Dec 2021 21:48:48: 9000000 INFO @ Wed, 08 Dec 2021 21:48:52: 4000000 INFO @ Wed, 08 Dec 2021 21:48:54: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 21:48:58: 5000000 INFO @ Wed, 08 Dec 2021 21:48:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 21:48:58: #1 read tag files... INFO @ Wed, 08 Dec 2021 21:48:58: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 21:49:00: 11000000 INFO @ Wed, 08 Dec 2021 21:49:04: 6000000 INFO @ Wed, 08 Dec 2021 21:49:04: 1000000 INFO @ Wed, 08 Dec 2021 21:49:05: 12000000 INFO @ Wed, 08 Dec 2021 21:49:09: 7000000 INFO @ Wed, 08 Dec 2021 21:49:10: 2000000 INFO @ Wed, 08 Dec 2021 21:49:11: 13000000 INFO @ Wed, 08 Dec 2021 21:49:15: 8000000 INFO @ Wed, 08 Dec 2021 21:49:16: 3000000 INFO @ Wed, 08 Dec 2021 21:49:17: 14000000 INFO @ Wed, 08 Dec 2021 21:49:21: 9000000 INFO @ Wed, 08 Dec 2021 21:49:22: 4000000 INFO @ Wed, 08 Dec 2021 21:49:23: 15000000 INFO @ Wed, 08 Dec 2021 21:49:27: 10000000 INFO @ Wed, 08 Dec 2021 21:49:28: 5000000 INFO @ Wed, 08 Dec 2021 21:49:29: 16000000 INFO @ Wed, 08 Dec 2021 21:49:33: 11000000 INFO @ Wed, 08 Dec 2021 21:49:34: 6000000 INFO @ Wed, 08 Dec 2021 21:49:35: 17000000 INFO @ Wed, 08 Dec 2021 21:49:39: 12000000 INFO @ Wed, 08 Dec 2021 21:49:39: 7000000 INFO @ Wed, 08 Dec 2021 21:49:41: 18000000 INFO @ Wed, 08 Dec 2021 21:49:45: 13000000 INFO @ Wed, 08 Dec 2021 21:49:45: 8000000 INFO @ Wed, 08 Dec 2021 21:49:47: 19000000 INFO @ Wed, 08 Dec 2021 21:49:51: 14000000 INFO @ Wed, 08 Dec 2021 21:49:51: 9000000 INFO @ Wed, 08 Dec 2021 21:49:53: 20000000 INFO @ Wed, 08 Dec 2021 21:49:57: 15000000 INFO @ Wed, 08 Dec 2021 21:49:57: 10000000 INFO @ Wed, 08 Dec 2021 21:49:59: 21000000 INFO @ Wed, 08 Dec 2021 21:50:03: 16000000 INFO @ Wed, 08 Dec 2021 21:50:03: 11000000 INFO @ Wed, 08 Dec 2021 21:50:04: 22000000 INFO @ Wed, 08 Dec 2021 21:50:09: 17000000 INFO @ Wed, 08 Dec 2021 21:50:09: 12000000 INFO @ Wed, 08 Dec 2021 21:50:10: 23000000 INFO @ Wed, 08 Dec 2021 21:50:15: 18000000 INFO @ Wed, 08 Dec 2021 21:50:15: 13000000 INFO @ Wed, 08 Dec 2021 21:50:16: 24000000 INFO @ Wed, 08 Dec 2021 21:50:21: 19000000 INFO @ Wed, 08 Dec 2021 21:50:21: 14000000 INFO @ Wed, 08 Dec 2021 21:50:22: 25000000 INFO @ Wed, 08 Dec 2021 21:50:26: 20000000 INFO @ Wed, 08 Dec 2021 21:50:27: 15000000 INFO @ Wed, 08 Dec 2021 21:50:28: 26000000 INFO @ Wed, 08 Dec 2021 21:50:32: 16000000 INFO @ Wed, 08 Dec 2021 21:50:32: 21000000 INFO @ Wed, 08 Dec 2021 21:50:34: 27000000 INFO @ Wed, 08 Dec 2021 21:50:38: 17000000 INFO @ Wed, 08 Dec 2021 21:50:38: 22000000 INFO @ Wed, 08 Dec 2021 21:50:40: 28000000 INFO @ Wed, 08 Dec 2021 21:50:44: 18000000 INFO @ Wed, 08 Dec 2021 21:50:44: 23000000 INFO @ Wed, 08 Dec 2021 21:50:45: 29000000 INFO @ Wed, 08 Dec 2021 21:50:50: 19000000 INFO @ Wed, 08 Dec 2021 21:50:50: 24000000 INFO @ Wed, 08 Dec 2021 21:50:51: 30000000 INFO @ Wed, 08 Dec 2021 21:50:54: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 21:50:54: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 21:50:54: #1 total tags in treatment: 30379885 INFO @ Wed, 08 Dec 2021 21:50:54: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 21:50:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 21:50:54: #1 tags after filtering in treatment: 30379885 INFO @ Wed, 08 Dec 2021 21:50:54: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 21:50:54: #1 finished! INFO @ Wed, 08 Dec 2021 21:50:54: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 21:50:54: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 21:50:56: 20000000 INFO @ Wed, 08 Dec 2021 21:50:56: 25000000 INFO @ Wed, 08 Dec 2021 21:50:56: #2 number of paired peaks: 98 WARNING @ Wed, 08 Dec 2021 21:50:56: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 21:50:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 21:51:01: 21000000 INFO @ Wed, 08 Dec 2021 21:51:01: 26000000 INFO @ Wed, 08 Dec 2021 21:51:07: 22000000 INFO @ Wed, 08 Dec 2021 21:51:07: 27000000 INFO @ Wed, 08 Dec 2021 21:51:13: 23000000 INFO @ Wed, 08 Dec 2021 21:51:13: 28000000 INFO @ Wed, 08 Dec 2021 21:51:19: 24000000 INFO @ Wed, 08 Dec 2021 21:51:19: 29000000 INFO @ Wed, 08 Dec 2021 21:51:24: 25000000 INFO @ Wed, 08 Dec 2021 21:51:24: 30000000 INFO @ Wed, 08 Dec 2021 21:51:27: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 21:51:27: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 21:51:27: #1 total tags in treatment: 30379885 INFO @ Wed, 08 Dec 2021 21:51:27: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 21:51:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 21:51:27: #1 tags after filtering in treatment: 30379885 INFO @ Wed, 08 Dec 2021 21:51:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 21:51:27: #1 finished! INFO @ Wed, 08 Dec 2021 21:51:27: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 21:51:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 21:51:29: #2 number of paired peaks: 98 WARNING @ Wed, 08 Dec 2021 21:51:29: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 21:51:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 21:51:30: 26000000 INFO @ Wed, 08 Dec 2021 21:51:35: 27000000 INFO @ Wed, 08 Dec 2021 21:51:41: 28000000 INFO @ Wed, 08 Dec 2021 21:51:46: 29000000 INFO @ Wed, 08 Dec 2021 21:51:51: 30000000 INFO @ Wed, 08 Dec 2021 21:51:53: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 21:51:53: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 21:51:53: #1 total tags in treatment: 30379885 INFO @ Wed, 08 Dec 2021 21:51:53: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 21:51:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 21:51:54: #1 tags after filtering in treatment: 30379885 INFO @ Wed, 08 Dec 2021 21:51:54: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 21:51:54: #1 finished! INFO @ Wed, 08 Dec 2021 21:51:54: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 21:51:54: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 21:51:56: #2 number of paired peaks: 98 WARNING @ Wed, 08 Dec 2021 21:51:56: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 21:51:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX9120610/SRX9120610.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling