Job ID = 14159345 SRX = SRX8948261 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:49:04 66904015 reads; of these: 66904015 (100.00%) were paired; of these: 21890508 (32.72%) aligned concordantly 0 times 38777582 (57.96%) aligned concordantly exactly 1 time 6235925 (9.32%) aligned concordantly >1 times ---- 21890508 pairs aligned concordantly 0 times; of these: 8619413 (39.38%) aligned discordantly 1 time ---- 13271095 pairs aligned 0 times concordantly or discordantly; of these: 26542190 mates make up the pairs; of these: 23953127 (90.25%) aligned 0 times 1021820 (3.85%) aligned exactly 1 time 1567243 (5.90%) aligned >1 times 82.10% overall alignment rate Time searching: 01:49:04 Overall time: 01:49:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 80 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 13726801 / 53264046 = 0.2577 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 23:29:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 23:29:04: #1 read tag files... INFO @ Wed, 08 Dec 2021 23:29:04: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 23:29:11: 1000000 INFO @ Wed, 08 Dec 2021 23:29:17: 2000000 INFO @ Wed, 08 Dec 2021 23:29:23: 3000000 INFO @ Wed, 08 Dec 2021 23:29:30: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 23:29:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 23:29:34: #1 read tag files... INFO @ Wed, 08 Dec 2021 23:29:34: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 23:29:36: 5000000 INFO @ Wed, 08 Dec 2021 23:29:41: 1000000 INFO @ Wed, 08 Dec 2021 23:29:43: 6000000 INFO @ Wed, 08 Dec 2021 23:29:48: 2000000 INFO @ Wed, 08 Dec 2021 23:29:50: 7000000 INFO @ Wed, 08 Dec 2021 23:29:55: 3000000 INFO @ Wed, 08 Dec 2021 23:29:57: 8000000 INFO @ Wed, 08 Dec 2021 23:30:02: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 23:30:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 23:30:04: #1 read tag files... INFO @ Wed, 08 Dec 2021 23:30:04: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 23:30:04: 9000000 INFO @ Wed, 08 Dec 2021 23:30:09: 5000000 INFO @ Wed, 08 Dec 2021 23:30:12: 10000000 INFO @ Wed, 08 Dec 2021 23:30:13: 1000000 INFO @ Wed, 08 Dec 2021 23:30:16: 6000000 INFO @ Wed, 08 Dec 2021 23:30:19: 11000000 INFO @ Wed, 08 Dec 2021 23:30:22: 2000000 INFO @ Wed, 08 Dec 2021 23:30:24: 7000000 INFO @ Wed, 08 Dec 2021 23:30:26: 12000000 INFO @ Wed, 08 Dec 2021 23:30:31: 3000000 INFO @ Wed, 08 Dec 2021 23:30:31: 8000000 INFO @ Wed, 08 Dec 2021 23:30:34: 13000000 INFO @ Wed, 08 Dec 2021 23:30:39: 9000000 INFO @ Wed, 08 Dec 2021 23:30:40: 4000000 INFO @ Wed, 08 Dec 2021 23:30:41: 14000000 INFO @ Wed, 08 Dec 2021 23:30:46: 10000000 INFO @ Wed, 08 Dec 2021 23:30:48: 15000000 INFO @ Wed, 08 Dec 2021 23:30:48: 5000000 INFO @ Wed, 08 Dec 2021 23:30:53: 11000000 INFO @ Wed, 08 Dec 2021 23:30:56: 16000000 INFO @ Wed, 08 Dec 2021 23:30:57: 6000000 INFO @ Wed, 08 Dec 2021 23:31:01: 12000000 INFO @ Wed, 08 Dec 2021 23:31:03: 17000000 INFO @ Wed, 08 Dec 2021 23:31:06: 7000000 INFO @ Wed, 08 Dec 2021 23:31:08: 13000000 INFO @ Wed, 08 Dec 2021 23:31:10: 18000000 INFO @ Wed, 08 Dec 2021 23:31:15: 8000000 INFO @ Wed, 08 Dec 2021 23:31:15: 14000000 INFO @ Wed, 08 Dec 2021 23:31:18: 19000000 INFO @ Wed, 08 Dec 2021 23:31:23: 15000000 INFO @ Wed, 08 Dec 2021 23:31:24: 9000000 INFO @ Wed, 08 Dec 2021 23:31:25: 20000000 INFO @ Wed, 08 Dec 2021 23:31:30: 16000000 INFO @ Wed, 08 Dec 2021 23:31:33: 10000000 INFO @ Wed, 08 Dec 2021 23:31:33: 21000000 INFO @ Wed, 08 Dec 2021 23:31:37: 17000000 INFO @ Wed, 08 Dec 2021 23:31:40: 22000000 INFO @ Wed, 08 Dec 2021 23:31:42: 11000000 INFO @ Wed, 08 Dec 2021 23:31:45: 18000000 INFO @ Wed, 08 Dec 2021 23:31:48: 23000000 INFO @ Wed, 08 Dec 2021 23:31:51: 12000000 INFO @ Wed, 08 Dec 2021 23:31:52: 19000000 INFO @ Wed, 08 Dec 2021 23:31:55: 24000000 INFO @ Wed, 08 Dec 2021 23:31:59: 20000000 INFO @ Wed, 08 Dec 2021 23:31:59: 13000000 INFO @ Wed, 08 Dec 2021 23:32:03: 25000000 INFO @ Wed, 08 Dec 2021 23:32:07: 21000000 INFO @ Wed, 08 Dec 2021 23:32:08: 14000000 INFO @ Wed, 08 Dec 2021 23:32:10: 26000000 INFO @ Wed, 08 Dec 2021 23:32:14: 22000000 INFO @ Wed, 08 Dec 2021 23:32:17: 15000000 INFO @ Wed, 08 Dec 2021 23:32:17: 27000000 INFO @ Wed, 08 Dec 2021 23:32:21: 23000000 INFO @ Wed, 08 Dec 2021 23:32:25: 28000000 INFO @ Wed, 08 Dec 2021 23:32:26: 16000000 INFO @ Wed, 08 Dec 2021 23:32:29: 24000000 INFO @ Wed, 08 Dec 2021 23:32:32: 29000000 INFO @ Wed, 08 Dec 2021 23:32:35: 17000000 INFO @ Wed, 08 Dec 2021 23:32:36: 25000000 INFO @ Wed, 08 Dec 2021 23:32:40: 30000000 INFO @ Wed, 08 Dec 2021 23:32:43: 26000000 INFO @ Wed, 08 Dec 2021 23:32:44: 18000000 INFO @ Wed, 08 Dec 2021 23:32:47: 31000000 INFO @ Wed, 08 Dec 2021 23:32:51: 27000000 INFO @ Wed, 08 Dec 2021 23:32:53: 19000000 INFO @ Wed, 08 Dec 2021 23:32:54: 32000000 INFO @ Wed, 08 Dec 2021 23:32:58: 28000000 INFO @ Wed, 08 Dec 2021 23:33:01: 20000000 INFO @ Wed, 08 Dec 2021 23:33:02: 33000000 INFO @ Wed, 08 Dec 2021 23:33:06: 29000000 INFO @ Wed, 08 Dec 2021 23:33:09: 34000000 INFO @ Wed, 08 Dec 2021 23:33:10: 21000000 INFO @ Wed, 08 Dec 2021 23:33:13: 30000000 INFO @ Wed, 08 Dec 2021 23:33:17: 35000000 INFO @ Wed, 08 Dec 2021 23:33:19: 22000000 INFO @ Wed, 08 Dec 2021 23:33:20: 31000000 INFO @ Wed, 08 Dec 2021 23:33:24: 36000000 INFO @ Wed, 08 Dec 2021 23:33:28: 32000000 INFO @ Wed, 08 Dec 2021 23:33:28: 23000000 INFO @ Wed, 08 Dec 2021 23:33:31: 37000000 INFO @ Wed, 08 Dec 2021 23:33:35: 33000000 INFO @ Wed, 08 Dec 2021 23:33:37: 24000000 INFO @ Wed, 08 Dec 2021 23:33:39: 38000000 INFO @ Wed, 08 Dec 2021 23:33:43: 34000000 INFO @ Wed, 08 Dec 2021 23:33:46: 25000000 INFO @ Wed, 08 Dec 2021 23:33:46: 39000000 INFO @ Wed, 08 Dec 2021 23:33:50: 35000000 INFO @ Wed, 08 Dec 2021 23:33:54: 40000000 INFO @ Wed, 08 Dec 2021 23:33:55: 26000000 INFO @ Wed, 08 Dec 2021 23:33:58: 36000000 INFO @ Wed, 08 Dec 2021 23:34:01: 41000000 INFO @ Wed, 08 Dec 2021 23:34:04: 27000000 INFO @ Wed, 08 Dec 2021 23:34:05: 37000000 INFO @ Wed, 08 Dec 2021 23:34:09: 42000000 INFO @ Wed, 08 Dec 2021 23:34:12: 38000000 INFO @ Wed, 08 Dec 2021 23:34:13: 28000000 INFO @ Wed, 08 Dec 2021 23:34:16: 43000000 INFO @ Wed, 08 Dec 2021 23:34:20: 39000000 INFO @ Wed, 08 Dec 2021 23:34:22: 29000000 INFO @ Wed, 08 Dec 2021 23:34:24: 44000000 INFO @ Wed, 08 Dec 2021 23:34:27: 40000000 INFO @ Wed, 08 Dec 2021 23:34:31: 30000000 INFO @ Wed, 08 Dec 2021 23:34:31: 45000000 INFO @ Wed, 08 Dec 2021 23:34:35: 41000000 INFO @ Wed, 08 Dec 2021 23:34:38: 46000000 INFO @ Wed, 08 Dec 2021 23:34:39: 31000000 INFO @ Wed, 08 Dec 2021 23:34:42: 42000000 INFO @ Wed, 08 Dec 2021 23:34:46: 47000000 INFO @ Wed, 08 Dec 2021 23:34:48: 32000000 INFO @ Wed, 08 Dec 2021 23:34:50: 43000000 INFO @ Wed, 08 Dec 2021 23:34:53: 48000000 INFO @ Wed, 08 Dec 2021 23:34:57: 44000000 INFO @ Wed, 08 Dec 2021 23:34:57: 33000000 INFO @ Wed, 08 Dec 2021 23:35:01: 49000000 INFO @ Wed, 08 Dec 2021 23:35:04: 45000000 INFO @ Wed, 08 Dec 2021 23:35:06: 34000000 INFO @ Wed, 08 Dec 2021 23:35:08: 50000000 INFO @ Wed, 08 Dec 2021 23:35:12: 46000000 INFO @ Wed, 08 Dec 2021 23:35:15: 35000000 INFO @ Wed, 08 Dec 2021 23:35:16: 51000000 INFO @ Wed, 08 Dec 2021 23:35:19: 47000000 INFO @ Wed, 08 Dec 2021 23:35:23: 52000000 INFO @ Wed, 08 Dec 2021 23:35:24: 36000000 INFO @ Wed, 08 Dec 2021 23:35:27: 48000000 INFO @ Wed, 08 Dec 2021 23:35:30: 53000000 INFO @ Wed, 08 Dec 2021 23:35:33: 37000000 INFO @ Wed, 08 Dec 2021 23:35:34: 49000000 INFO @ Wed, 08 Dec 2021 23:35:38: 54000000 INFO @ Wed, 08 Dec 2021 23:35:42: 50000000 INFO @ Wed, 08 Dec 2021 23:35:42: 38000000 INFO @ Wed, 08 Dec 2021 23:35:45: 55000000 INFO @ Wed, 08 Dec 2021 23:35:49: 51000000 INFO @ Wed, 08 Dec 2021 23:35:51: 39000000 INFO @ Wed, 08 Dec 2021 23:35:53: 56000000 INFO @ Wed, 08 Dec 2021 23:35:56: 52000000 INFO @ Wed, 08 Dec 2021 23:36:00: 40000000 INFO @ Wed, 08 Dec 2021 23:36:00: 57000000 INFO @ Wed, 08 Dec 2021 23:36:04: 53000000 INFO @ Wed, 08 Dec 2021 23:36:07: 58000000 INFO @ Wed, 08 Dec 2021 23:36:08: 41000000 INFO @ Wed, 08 Dec 2021 23:36:11: 54000000 INFO @ Wed, 08 Dec 2021 23:36:14: 59000000 INFO @ Wed, 08 Dec 2021 23:36:17: 42000000 INFO @ Wed, 08 Dec 2021 23:36:19: 55000000 INFO @ Wed, 08 Dec 2021 23:36:22: 60000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 23:36:26: 56000000 INFO @ Wed, 08 Dec 2021 23:36:26: 43000000 INFO @ Wed, 08 Dec 2021 23:36:29: 61000000 INFO @ Wed, 08 Dec 2021 23:36:33: 57000000 INFO @ Wed, 08 Dec 2021 23:36:35: 44000000 INFO @ Wed, 08 Dec 2021 23:36:36: 62000000 INFO @ Wed, 08 Dec 2021 23:36:41: 58000000 INFO @ Wed, 08 Dec 2021 23:36:44: 63000000 INFO @ Wed, 08 Dec 2021 23:36:44: 45000000 INFO @ Wed, 08 Dec 2021 23:36:48: 59000000 INFO @ Wed, 08 Dec 2021 23:36:51: 64000000 INFO @ Wed, 08 Dec 2021 23:36:53: 46000000 INFO @ Wed, 08 Dec 2021 23:36:55: 60000000 INFO @ Wed, 08 Dec 2021 23:36:58: 65000000 INFO @ Wed, 08 Dec 2021 23:37:02: 47000000 INFO @ Wed, 08 Dec 2021 23:37:02: 61000000 INFO @ Wed, 08 Dec 2021 23:37:06: 66000000 INFO @ Wed, 08 Dec 2021 23:37:10: 62000000 INFO @ Wed, 08 Dec 2021 23:37:11: 48000000 INFO @ Wed, 08 Dec 2021 23:37:13: 67000000 INFO @ Wed, 08 Dec 2021 23:37:17: 63000000 INFO @ Wed, 08 Dec 2021 23:37:20: 49000000 INFO @ Wed, 08 Dec 2021 23:37:20: 68000000 INFO @ Wed, 08 Dec 2021 23:37:24: 64000000 INFO @ Wed, 08 Dec 2021 23:37:28: 69000000 INFO @ Wed, 08 Dec 2021 23:37:29: 50000000 INFO @ Wed, 08 Dec 2021 23:37:32: 65000000 INFO @ Wed, 08 Dec 2021 23:37:35: 70000000 INFO @ Wed, 08 Dec 2021 23:37:38: 51000000 INFO @ Wed, 08 Dec 2021 23:37:39: 66000000 INFO @ Wed, 08 Dec 2021 23:37:42: 71000000 INFO @ Wed, 08 Dec 2021 23:37:46: 52000000 INFO @ Wed, 08 Dec 2021 23:37:47: 67000000 INFO @ Wed, 08 Dec 2021 23:37:50: 72000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 23:37:54: 68000000 INFO @ Wed, 08 Dec 2021 23:37:55: 53000000 INFO @ Wed, 08 Dec 2021 23:37:57: 73000000 INFO @ Wed, 08 Dec 2021 23:38:01: 69000000 INFO @ Wed, 08 Dec 2021 23:38:04: 54000000 INFO @ Wed, 08 Dec 2021 23:38:04: 74000000 INFO @ Wed, 08 Dec 2021 23:38:09: 70000000 INFO @ Wed, 08 Dec 2021 23:38:12: 75000000 INFO @ Wed, 08 Dec 2021 23:38:13: 55000000 INFO @ Wed, 08 Dec 2021 23:38:16: 71000000 INFO @ Wed, 08 Dec 2021 23:38:19: 76000000 INFO @ Wed, 08 Dec 2021 23:38:22: 56000000 INFO @ Wed, 08 Dec 2021 23:38:23: 72000000 INFO @ Wed, 08 Dec 2021 23:38:26: 77000000 INFO @ Wed, 08 Dec 2021 23:38:31: 73000000 INFO @ Wed, 08 Dec 2021 23:38:31: 57000000 INFO @ Wed, 08 Dec 2021 23:38:34: 78000000 INFO @ Wed, 08 Dec 2021 23:38:38: 74000000 INFO @ Wed, 08 Dec 2021 23:38:39: 58000000 INFO @ Wed, 08 Dec 2021 23:38:41: 79000000 INFO @ Wed, 08 Dec 2021 23:38:45: 75000000 INFO @ Wed, 08 Dec 2021 23:38:48: 59000000 INFO @ Wed, 08 Dec 2021 23:38:49: 80000000 INFO @ Wed, 08 Dec 2021 23:38:53: 76000000 INFO @ Wed, 08 Dec 2021 23:38:56: 81000000 INFO @ Wed, 08 Dec 2021 23:38:56: 60000000 INFO @ Wed, 08 Dec 2021 23:39:00: 77000000 INFO @ Wed, 08 Dec 2021 23:39:03: 82000000 INFO @ Wed, 08 Dec 2021 23:39:05: 61000000 INFO @ Wed, 08 Dec 2021 23:39:07: #1 tag size is determined as 150 bps INFO @ Wed, 08 Dec 2021 23:39:07: #1 tag size = 150 INFO @ Wed, 08 Dec 2021 23:39:07: #1 total tags in treatment: 32961281 INFO @ Wed, 08 Dec 2021 23:39:07: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 23:39:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 23:39:07: #1 tags after filtering in treatment: 28456796 INFO @ Wed, 08 Dec 2021 23:39:07: #1 Redundant rate of treatment: 0.14 INFO @ Wed, 08 Dec 2021 23:39:07: #1 finished! INFO @ Wed, 08 Dec 2021 23:39:07: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 23:39:07: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 23:39:08: 78000000 INFO @ Wed, 08 Dec 2021 23:39:09: #2 number of paired peaks: 134 WARNING @ Wed, 08 Dec 2021 23:39:09: Fewer paired peaks (134) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 134 pairs to build model! INFO @ Wed, 08 Dec 2021 23:39:09: start model_add_line... INFO @ Wed, 08 Dec 2021 23:39:09: start X-correlation... INFO @ Wed, 08 Dec 2021 23:39:09: end of X-cor INFO @ Wed, 08 Dec 2021 23:39:09: #2 finished! INFO @ Wed, 08 Dec 2021 23:39:09: #2 predicted fragment length is 145 bps INFO @ Wed, 08 Dec 2021 23:39:09: #2 alternative fragment length(s) may be 2,106,145 bps INFO @ Wed, 08 Dec 2021 23:39:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.05_model.r WARNING @ Wed, 08 Dec 2021 23:39:09: #2 Since the d (145) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 08 Dec 2021 23:39:09: #2 You may need to consider one of the other alternative d(s): 2,106,145 WARNING @ Wed, 08 Dec 2021 23:39:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 08 Dec 2021 23:39:09: #3 Call peaks... INFO @ Wed, 08 Dec 2021 23:39:09: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 23:39:13: 62000000 INFO @ Wed, 08 Dec 2021 23:39:15: 79000000 INFO @ Wed, 08 Dec 2021 23:39:22: 80000000 INFO @ Wed, 08 Dec 2021 23:39:22: 63000000 INFO @ Wed, 08 Dec 2021 23:39:29: 81000000 INFO @ Wed, 08 Dec 2021 23:39:30: 64000000 INFO @ Wed, 08 Dec 2021 23:39:37: 82000000 INFO @ Wed, 08 Dec 2021 23:39:39: 65000000 INFO @ Wed, 08 Dec 2021 23:39:40: #1 tag size is determined as 150 bps INFO @ Wed, 08 Dec 2021 23:39:40: #1 tag size = 150 INFO @ Wed, 08 Dec 2021 23:39:40: #1 total tags in treatment: 32961281 INFO @ Wed, 08 Dec 2021 23:39:40: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 23:39:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 23:39:40: #1 tags after filtering in treatment: 28456796 INFO @ Wed, 08 Dec 2021 23:39:40: #1 Redundant rate of treatment: 0.14 INFO @ Wed, 08 Dec 2021 23:39:40: #1 finished! INFO @ Wed, 08 Dec 2021 23:39:40: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 23:39:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 23:39:42: #2 number of paired peaks: 134 WARNING @ Wed, 08 Dec 2021 23:39:42: Fewer paired peaks (134) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 134 pairs to build model! INFO @ Wed, 08 Dec 2021 23:39:42: start model_add_line... INFO @ Wed, 08 Dec 2021 23:39:42: start X-correlation... INFO @ Wed, 08 Dec 2021 23:39:42: end of X-cor INFO @ Wed, 08 Dec 2021 23:39:42: #2 finished! INFO @ Wed, 08 Dec 2021 23:39:42: #2 predicted fragment length is 145 bps INFO @ Wed, 08 Dec 2021 23:39:42: #2 alternative fragment length(s) may be 2,106,145 bps INFO @ Wed, 08 Dec 2021 23:39:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.10_model.r WARNING @ Wed, 08 Dec 2021 23:39:42: #2 Since the d (145) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 08 Dec 2021 23:39:42: #2 You may need to consider one of the other alternative d(s): 2,106,145 WARNING @ Wed, 08 Dec 2021 23:39:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 08 Dec 2021 23:39:42: #3 Call peaks... INFO @ Wed, 08 Dec 2021 23:39:42: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 23:39:47: 66000000 INFO @ Wed, 08 Dec 2021 23:39:52: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 23:39:56: 67000000 INFO @ Wed, 08 Dec 2021 23:40:04: 68000000 INFO @ Wed, 08 Dec 2021 23:40:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.05_peaks.xls INFO @ Wed, 08 Dec 2021 23:40:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.05_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 23:40:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.05_summits.bed INFO @ Wed, 08 Dec 2021 23:40:11: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (588 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 23:40:12: 69000000 INFO @ Wed, 08 Dec 2021 23:40:20: 70000000 INFO @ Wed, 08 Dec 2021 23:40:25: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 23:40:28: 71000000 INFO @ Wed, 08 Dec 2021 23:40:36: 72000000 INFO @ Wed, 08 Dec 2021 23:40:44: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.10_peaks.xls INFO @ Wed, 08 Dec 2021 23:40:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.10_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 23:40:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.10_summits.bed INFO @ Wed, 08 Dec 2021 23:40:44: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (433 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 23:40:44: 73000000 INFO @ Wed, 08 Dec 2021 23:40:52: 74000000 INFO @ Wed, 08 Dec 2021 23:41:00: 75000000 INFO @ Wed, 08 Dec 2021 23:41:08: 76000000 INFO @ Wed, 08 Dec 2021 23:41:16: 77000000 INFO @ Wed, 08 Dec 2021 23:41:24: 78000000 INFO @ Wed, 08 Dec 2021 23:41:32: 79000000 INFO @ Wed, 08 Dec 2021 23:41:40: 80000000 INFO @ Wed, 08 Dec 2021 23:41:49: 81000000 INFO @ Wed, 08 Dec 2021 23:41:57: 82000000 INFO @ Wed, 08 Dec 2021 23:42:00: #1 tag size is determined as 150 bps INFO @ Wed, 08 Dec 2021 23:42:00: #1 tag size = 150 INFO @ Wed, 08 Dec 2021 23:42:00: #1 total tags in treatment: 32961281 INFO @ Wed, 08 Dec 2021 23:42:00: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 23:42:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 23:42:01: #1 tags after filtering in treatment: 28456796 INFO @ Wed, 08 Dec 2021 23:42:01: #1 Redundant rate of treatment: 0.14 INFO @ Wed, 08 Dec 2021 23:42:01: #1 finished! INFO @ Wed, 08 Dec 2021 23:42:01: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 23:42:01: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 23:42:03: #2 number of paired peaks: 134 WARNING @ Wed, 08 Dec 2021 23:42:03: Fewer paired peaks (134) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 134 pairs to build model! INFO @ Wed, 08 Dec 2021 23:42:03: start model_add_line... INFO @ Wed, 08 Dec 2021 23:42:03: start X-correlation... INFO @ Wed, 08 Dec 2021 23:42:03: end of X-cor INFO @ Wed, 08 Dec 2021 23:42:03: #2 finished! INFO @ Wed, 08 Dec 2021 23:42:03: #2 predicted fragment length is 145 bps INFO @ Wed, 08 Dec 2021 23:42:03: #2 alternative fragment length(s) may be 2,106,145 bps INFO @ Wed, 08 Dec 2021 23:42:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.20_model.r WARNING @ Wed, 08 Dec 2021 23:42:03: #2 Since the d (145) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 08 Dec 2021 23:42:03: #2 You may need to consider one of the other alternative d(s): 2,106,145 WARNING @ Wed, 08 Dec 2021 23:42:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 08 Dec 2021 23:42:03: #3 Call peaks... INFO @ Wed, 08 Dec 2021 23:42:03: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 23:42:45: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 23:43:04: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.20_peaks.xls INFO @ Wed, 08 Dec 2021 23:43:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.20_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 23:43:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8948261/SRX8948261.20_summits.bed INFO @ Wed, 08 Dec 2021 23:43:04: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (283 records, 4 fields): 1 millis CompletedMACS2peakCalling