Job ID = 14159284 SRX = SRX8948249 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:00:45 60580808 reads; of these: 60580808 (100.00%) were paired; of these: 31839466 (52.56%) aligned concordantly 0 times 24263513 (40.05%) aligned concordantly exactly 1 time 4477829 (7.39%) aligned concordantly >1 times ---- 31839466 pairs aligned concordantly 0 times; of these: 9830502 (30.88%) aligned discordantly 1 time ---- 22008964 pairs aligned 0 times concordantly or discordantly; of these: 44017928 mates make up the pairs; of these: 40997856 (93.14%) aligned 0 times 1176843 (2.67%) aligned exactly 1 time 1843229 (4.19%) aligned >1 times 66.16% overall alignment rate Time searching: 02:00:45 Overall time: 02:00:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 60 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 9039398 / 38200916 = 0.2366 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 23:15:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 23:15:42: #1 read tag files... INFO @ Wed, 08 Dec 2021 23:15:42: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 23:15:53: 1000000 INFO @ Wed, 08 Dec 2021 23:16:05: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 23:16:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 23:16:10: #1 read tag files... INFO @ Wed, 08 Dec 2021 23:16:10: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 23:16:17: 3000000 INFO @ Wed, 08 Dec 2021 23:16:25: 1000000 INFO @ Wed, 08 Dec 2021 23:16:29: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 23:16:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 23:16:40: #1 read tag files... INFO @ Wed, 08 Dec 2021 23:16:40: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 23:16:41: 2000000 INFO @ Wed, 08 Dec 2021 23:16:41: 5000000 INFO @ Wed, 08 Dec 2021 23:16:51: 1000000 INFO @ Wed, 08 Dec 2021 23:16:54: 6000000 INFO @ Wed, 08 Dec 2021 23:16:57: 3000000 INFO @ Wed, 08 Dec 2021 23:17:01: 2000000 INFO @ Wed, 08 Dec 2021 23:17:06: 7000000 INFO @ Wed, 08 Dec 2021 23:17:12: 3000000 INFO @ Wed, 08 Dec 2021 23:17:12: 4000000 INFO @ Wed, 08 Dec 2021 23:17:18: 8000000 INFO @ Wed, 08 Dec 2021 23:17:22: 4000000 INFO @ Wed, 08 Dec 2021 23:17:28: 5000000 INFO @ Wed, 08 Dec 2021 23:17:30: 9000000 INFO @ Wed, 08 Dec 2021 23:17:31: 5000000 INFO @ Wed, 08 Dec 2021 23:17:42: 6000000 INFO @ Wed, 08 Dec 2021 23:17:42: 10000000 INFO @ Wed, 08 Dec 2021 23:17:44: 6000000 INFO @ Wed, 08 Dec 2021 23:17:52: 7000000 INFO @ Wed, 08 Dec 2021 23:17:54: 11000000 INFO @ Wed, 08 Dec 2021 23:17:59: 7000000 INFO @ Wed, 08 Dec 2021 23:18:02: 8000000 INFO @ Wed, 08 Dec 2021 23:18:06: 12000000 INFO @ Wed, 08 Dec 2021 23:18:12: 9000000 INFO @ Wed, 08 Dec 2021 23:18:15: 8000000 INFO @ Wed, 08 Dec 2021 23:18:19: 13000000 INFO @ Wed, 08 Dec 2021 23:18:23: 10000000 INFO @ Wed, 08 Dec 2021 23:18:31: 9000000 INFO @ Wed, 08 Dec 2021 23:18:31: 14000000 INFO @ Wed, 08 Dec 2021 23:18:33: 11000000 INFO @ Wed, 08 Dec 2021 23:18:44: 15000000 INFO @ Wed, 08 Dec 2021 23:18:44: 12000000 INFO @ Wed, 08 Dec 2021 23:18:46: 10000000 INFO @ Wed, 08 Dec 2021 23:18:55: 13000000 INFO @ Wed, 08 Dec 2021 23:18:56: 16000000 INFO @ Wed, 08 Dec 2021 23:19:02: 11000000 INFO @ Wed, 08 Dec 2021 23:19:05: 14000000 INFO @ Wed, 08 Dec 2021 23:19:09: 17000000 INFO @ Wed, 08 Dec 2021 23:19:15: 15000000 INFO @ Wed, 08 Dec 2021 23:19:17: 12000000 INFO @ Wed, 08 Dec 2021 23:19:21: 18000000 INFO @ Wed, 08 Dec 2021 23:19:25: 16000000 INFO @ Wed, 08 Dec 2021 23:19:33: 13000000 INFO @ Wed, 08 Dec 2021 23:19:34: 19000000 INFO @ Wed, 08 Dec 2021 23:19:35: 17000000 INFO @ Wed, 08 Dec 2021 23:19:45: 18000000 INFO @ Wed, 08 Dec 2021 23:19:46: 20000000 INFO @ Wed, 08 Dec 2021 23:19:49: 14000000 INFO @ Wed, 08 Dec 2021 23:19:56: 19000000 INFO @ Wed, 08 Dec 2021 23:19:58: 21000000 INFO @ Wed, 08 Dec 2021 23:20:05: 15000000 INFO @ Wed, 08 Dec 2021 23:20:06: 20000000 INFO @ Wed, 08 Dec 2021 23:20:10: 22000000 INFO @ Wed, 08 Dec 2021 23:20:16: 21000000 INFO @ Wed, 08 Dec 2021 23:20:20: 16000000 INFO @ Wed, 08 Dec 2021 23:20:22: 23000000 INFO @ Wed, 08 Dec 2021 23:20:26: 22000000 INFO @ Wed, 08 Dec 2021 23:20:34: 17000000 INFO @ Wed, 08 Dec 2021 23:20:35: 24000000 INFO @ Wed, 08 Dec 2021 23:20:36: 23000000 INFO @ Wed, 08 Dec 2021 23:20:46: 24000000 INFO @ Wed, 08 Dec 2021 23:20:47: 25000000 INFO @ Wed, 08 Dec 2021 23:20:49: 18000000 INFO @ Wed, 08 Dec 2021 23:20:57: 25000000 INFO @ Wed, 08 Dec 2021 23:20:59: 26000000 INFO @ Wed, 08 Dec 2021 23:21:04: 19000000 INFO @ Wed, 08 Dec 2021 23:21:07: 26000000 INFO @ Wed, 08 Dec 2021 23:21:11: 27000000 INFO @ Wed, 08 Dec 2021 23:21:18: 27000000 INFO @ Wed, 08 Dec 2021 23:21:19: 20000000 INFO @ Wed, 08 Dec 2021 23:21:23: 28000000 INFO @ Wed, 08 Dec 2021 23:21:28: 28000000 INFO @ Wed, 08 Dec 2021 23:21:35: 29000000 INFO @ Wed, 08 Dec 2021 23:21:35: 21000000 INFO @ Wed, 08 Dec 2021 23:21:39: 29000000 INFO @ Wed, 08 Dec 2021 23:21:47: 30000000 INFO @ Wed, 08 Dec 2021 23:21:50: 30000000 INFO @ Wed, 08 Dec 2021 23:21:50: 22000000 INFO @ Wed, 08 Dec 2021 23:22:00: 31000000 INFO @ Wed, 08 Dec 2021 23:22:01: 31000000 INFO @ Wed, 08 Dec 2021 23:22:05: 23000000 INFO @ Wed, 08 Dec 2021 23:22:12: 32000000 INFO @ Wed, 08 Dec 2021 23:22:13: 32000000 INFO @ Wed, 08 Dec 2021 23:22:20: 24000000 INFO @ Wed, 08 Dec 2021 23:22:22: 33000000 INFO @ Wed, 08 Dec 2021 23:22:25: 33000000 INFO @ Wed, 08 Dec 2021 23:22:32: 34000000 INFO @ Wed, 08 Dec 2021 23:22:35: 25000000 INFO @ Wed, 08 Dec 2021 23:22:38: 34000000 INFO @ Wed, 08 Dec 2021 23:22:42: 35000000 INFO @ Wed, 08 Dec 2021 23:22:49: 26000000 INFO @ Wed, 08 Dec 2021 23:22:50: 35000000 INFO @ Wed, 08 Dec 2021 23:22:52: 36000000 INFO @ Wed, 08 Dec 2021 23:23:02: 36000000 INFO @ Wed, 08 Dec 2021 23:23:02: 37000000 INFO @ Wed, 08 Dec 2021 23:23:03: 27000000 INFO @ Wed, 08 Dec 2021 23:23:13: 38000000 INFO @ Wed, 08 Dec 2021 23:23:15: 37000000 INFO @ Wed, 08 Dec 2021 23:23:17: 28000000 INFO @ Wed, 08 Dec 2021 23:23:23: 39000000 INFO @ Wed, 08 Dec 2021 23:23:27: 38000000 INFO @ Wed, 08 Dec 2021 23:23:32: 29000000 INFO @ Wed, 08 Dec 2021 23:23:33: 40000000 INFO @ Wed, 08 Dec 2021 23:23:40: 39000000 INFO @ Wed, 08 Dec 2021 23:23:43: 41000000 INFO @ Wed, 08 Dec 2021 23:23:46: 30000000 INFO @ Wed, 08 Dec 2021 23:23:52: 40000000 INFO @ Wed, 08 Dec 2021 23:23:54: 42000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 23:24:02: 31000000 INFO @ Wed, 08 Dec 2021 23:24:04: 43000000 INFO @ Wed, 08 Dec 2021 23:24:05: 41000000 INFO @ Wed, 08 Dec 2021 23:24:14: 44000000 INFO @ Wed, 08 Dec 2021 23:24:16: 42000000 INFO @ Wed, 08 Dec 2021 23:24:18: 32000000 INFO @ Wed, 08 Dec 2021 23:24:24: 45000000 INFO @ Wed, 08 Dec 2021 23:24:28: 43000000 INFO @ Wed, 08 Dec 2021 23:24:32: 33000000 INFO @ Wed, 08 Dec 2021 23:24:34: 46000000 INFO @ Wed, 08 Dec 2021 23:24:40: 44000000 INFO @ Wed, 08 Dec 2021 23:24:43: 47000000 INFO @ Wed, 08 Dec 2021 23:24:47: 34000000 INFO @ Wed, 08 Dec 2021 23:24:51: 45000000 INFO @ Wed, 08 Dec 2021 23:24:53: 48000000 INFO @ Wed, 08 Dec 2021 23:25:02: 46000000 INFO @ Wed, 08 Dec 2021 23:25:03: 35000000 INFO @ Wed, 08 Dec 2021 23:25:03: 49000000 INFO @ Wed, 08 Dec 2021 23:25:13: 50000000 INFO @ Wed, 08 Dec 2021 23:25:13: 47000000 INFO @ Wed, 08 Dec 2021 23:25:18: 36000000 INFO @ Wed, 08 Dec 2021 23:25:23: 51000000 INFO @ Wed, 08 Dec 2021 23:25:25: 48000000 INFO @ Wed, 08 Dec 2021 23:25:33: 52000000 INFO @ Wed, 08 Dec 2021 23:25:34: 37000000 INFO @ Wed, 08 Dec 2021 23:25:36: 49000000 INFO @ Wed, 08 Dec 2021 23:25:43: 53000000 INFO @ Wed, 08 Dec 2021 23:25:48: 50000000 INFO @ Wed, 08 Dec 2021 23:25:49: 38000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 23:25:54: 54000000 INFO @ Wed, 08 Dec 2021 23:25:59: 51000000 INFO @ Wed, 08 Dec 2021 23:26:04: 39000000 INFO @ Wed, 08 Dec 2021 23:26:04: 55000000 INFO @ Wed, 08 Dec 2021 23:26:11: 52000000 INFO @ Wed, 08 Dec 2021 23:26:15: 56000000 INFO @ Wed, 08 Dec 2021 23:26:19: 40000000 INFO @ Wed, 08 Dec 2021 23:26:22: 53000000 INFO @ Wed, 08 Dec 2021 23:26:24: 57000000 INFO @ Wed, 08 Dec 2021 23:26:34: 58000000 INFO @ Wed, 08 Dec 2021 23:26:34: 54000000 INFO @ Wed, 08 Dec 2021 23:26:36: 41000000 INFO @ Wed, 08 Dec 2021 23:26:44: 59000000 INFO @ Wed, 08 Dec 2021 23:26:47: 55000000 INFO @ Wed, 08 Dec 2021 23:26:52: 42000000 INFO @ Wed, 08 Dec 2021 23:26:54: 60000000 INFO @ Wed, 08 Dec 2021 23:27:00: 56000000 INFO @ Wed, 08 Dec 2021 23:27:04: 61000000 INFO @ Wed, 08 Dec 2021 23:27:09: 43000000 INFO @ Wed, 08 Dec 2021 23:27:13: 57000000 INFO @ Wed, 08 Dec 2021 23:27:14: 62000000 INFO @ Wed, 08 Dec 2021 23:27:15: #1 tag size is determined as 150 bps INFO @ Wed, 08 Dec 2021 23:27:15: #1 tag size = 150 INFO @ Wed, 08 Dec 2021 23:27:15: #1 total tags in treatment: 21536618 INFO @ Wed, 08 Dec 2021 23:27:15: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 23:27:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 23:27:15: #1 tags after filtering in treatment: 18642469 INFO @ Wed, 08 Dec 2021 23:27:15: #1 Redundant rate of treatment: 0.13 INFO @ Wed, 08 Dec 2021 23:27:15: #1 finished! INFO @ Wed, 08 Dec 2021 23:27:15: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 23:27:15: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 23:27:17: #2 number of paired peaks: 258 WARNING @ Wed, 08 Dec 2021 23:27:17: Fewer paired peaks (258) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 258 pairs to build model! INFO @ Wed, 08 Dec 2021 23:27:17: start model_add_line... INFO @ Wed, 08 Dec 2021 23:27:17: start X-correlation... INFO @ Wed, 08 Dec 2021 23:27:17: end of X-cor INFO @ Wed, 08 Dec 2021 23:27:17: #2 finished! INFO @ Wed, 08 Dec 2021 23:27:17: #2 predicted fragment length is 192 bps INFO @ Wed, 08 Dec 2021 23:27:17: #2 alternative fragment length(s) may be 4,192 bps INFO @ Wed, 08 Dec 2021 23:27:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.20_model.r WARNING @ Wed, 08 Dec 2021 23:27:17: #2 Since the d (192) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 08 Dec 2021 23:27:17: #2 You may need to consider one of the other alternative d(s): 4,192 WARNING @ Wed, 08 Dec 2021 23:27:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 08 Dec 2021 23:27:17: #3 Call peaks... INFO @ Wed, 08 Dec 2021 23:27:17: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 23:27:26: 44000000 INFO @ Wed, 08 Dec 2021 23:27:27: 58000000 INFO @ Wed, 08 Dec 2021 23:27:40: 59000000 INFO @ Wed, 08 Dec 2021 23:27:42: 45000000 INFO @ Wed, 08 Dec 2021 23:27:53: 60000000 INFO @ Wed, 08 Dec 2021 23:27:58: 46000000 INFO @ Wed, 08 Dec 2021 23:28:06: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 23:28:06: 61000000 INFO @ Wed, 08 Dec 2021 23:28:14: 47000000 INFO @ Wed, 08 Dec 2021 23:28:19: 62000000 INFO @ Wed, 08 Dec 2021 23:28:20: #1 tag size is determined as 150 bps INFO @ Wed, 08 Dec 2021 23:28:20: #1 tag size = 150 INFO @ Wed, 08 Dec 2021 23:28:20: #1 total tags in treatment: 21536618 INFO @ Wed, 08 Dec 2021 23:28:20: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 23:28:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 23:28:21: #1 tags after filtering in treatment: 18642469 INFO @ Wed, 08 Dec 2021 23:28:21: #1 Redundant rate of treatment: 0.13 INFO @ Wed, 08 Dec 2021 23:28:21: #1 finished! INFO @ Wed, 08 Dec 2021 23:28:21: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 23:28:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 23:28:22: #2 number of paired peaks: 258 WARNING @ Wed, 08 Dec 2021 23:28:22: Fewer paired peaks (258) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 258 pairs to build model! INFO @ Wed, 08 Dec 2021 23:28:22: start model_add_line... INFO @ Wed, 08 Dec 2021 23:28:23: start X-correlation... INFO @ Wed, 08 Dec 2021 23:28:23: end of X-cor INFO @ Wed, 08 Dec 2021 23:28:23: #2 finished! INFO @ Wed, 08 Dec 2021 23:28:23: #2 predicted fragment length is 192 bps INFO @ Wed, 08 Dec 2021 23:28:23: #2 alternative fragment length(s) may be 4,192 bps INFO @ Wed, 08 Dec 2021 23:28:23: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.05_model.r WARNING @ Wed, 08 Dec 2021 23:28:23: #2 Since the d (192) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 08 Dec 2021 23:28:23: #2 You may need to consider one of the other alternative d(s): 4,192 WARNING @ Wed, 08 Dec 2021 23:28:23: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 08 Dec 2021 23:28:23: #3 Call peaks... INFO @ Wed, 08 Dec 2021 23:28:23: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 23:28:28: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.20_peaks.xls INFO @ Wed, 08 Dec 2021 23:28:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.20_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 23:28:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.20_summits.bed INFO @ Wed, 08 Dec 2021 23:28:28: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (290 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 23:28:31: 48000000 INFO @ Wed, 08 Dec 2021 23:28:48: 49000000 INFO @ Wed, 08 Dec 2021 23:29:04: 50000000 INFO @ Wed, 08 Dec 2021 23:29:12: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 23:29:20: 51000000 INFO @ Wed, 08 Dec 2021 23:29:34: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.05_peaks.xls INFO @ Wed, 08 Dec 2021 23:29:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.05_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 23:29:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.05_summits.bed INFO @ Wed, 08 Dec 2021 23:29:34: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (809 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 23:29:37: 52000000 INFO @ Wed, 08 Dec 2021 23:29:54: 53000000 INFO @ Wed, 08 Dec 2021 23:30:11: 54000000 INFO @ Wed, 08 Dec 2021 23:30:28: 55000000 INFO @ Wed, 08 Dec 2021 23:30:45: 56000000 INFO @ Wed, 08 Dec 2021 23:31:01: 57000000 INFO @ Wed, 08 Dec 2021 23:31:16: 58000000 INFO @ Wed, 08 Dec 2021 23:31:29: 59000000 INFO @ Wed, 08 Dec 2021 23:31:43: 60000000 INFO @ Wed, 08 Dec 2021 23:31:58: 61000000 INFO @ Wed, 08 Dec 2021 23:32:12: 62000000 INFO @ Wed, 08 Dec 2021 23:32:13: #1 tag size is determined as 150 bps INFO @ Wed, 08 Dec 2021 23:32:13: #1 tag size = 150 INFO @ Wed, 08 Dec 2021 23:32:13: #1 total tags in treatment: 21536618 INFO @ Wed, 08 Dec 2021 23:32:13: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 23:32:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 23:32:14: #1 tags after filtering in treatment: 18642469 INFO @ Wed, 08 Dec 2021 23:32:14: #1 Redundant rate of treatment: 0.13 INFO @ Wed, 08 Dec 2021 23:32:14: #1 finished! INFO @ Wed, 08 Dec 2021 23:32:14: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 23:32:14: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 23:32:15: #2 number of paired peaks: 258 WARNING @ Wed, 08 Dec 2021 23:32:15: Fewer paired peaks (258) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 258 pairs to build model! INFO @ Wed, 08 Dec 2021 23:32:15: start model_add_line... INFO @ Wed, 08 Dec 2021 23:32:15: start X-correlation... INFO @ Wed, 08 Dec 2021 23:32:15: end of X-cor INFO @ Wed, 08 Dec 2021 23:32:15: #2 finished! INFO @ Wed, 08 Dec 2021 23:32:15: #2 predicted fragment length is 192 bps INFO @ Wed, 08 Dec 2021 23:32:15: #2 alternative fragment length(s) may be 4,192 bps INFO @ Wed, 08 Dec 2021 23:32:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.10_model.r WARNING @ Wed, 08 Dec 2021 23:32:15: #2 Since the d (192) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 08 Dec 2021 23:32:15: #2 You may need to consider one of the other alternative d(s): 4,192 WARNING @ Wed, 08 Dec 2021 23:32:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 08 Dec 2021 23:32:15: #3 Call peaks... INFO @ Wed, 08 Dec 2021 23:32:15: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 23:33:05: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 23:33:28: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.10_peaks.xls INFO @ Wed, 08 Dec 2021 23:33:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.10_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 23:33:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8948249/SRX8948249.10_summits.bed INFO @ Wed, 08 Dec 2021 23:33:28: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (462 records, 4 fields): 2 millis CompletedMACS2peakCalling