Job ID = 14159944 SRX = SRX8845638 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:47:47 48875734 reads; of these: 48875734 (100.00%) were paired; of these: 4110057 (8.41%) aligned concordantly 0 times 35809893 (73.27%) aligned concordantly exactly 1 time 8955784 (18.32%) aligned concordantly >1 times ---- 4110057 pairs aligned concordantly 0 times; of these: 950392 (23.12%) aligned discordantly 1 time ---- 3159665 pairs aligned 0 times concordantly or discordantly; of these: 6319330 mates make up the pairs; of these: 5269706 (83.39%) aligned 0 times 437366 (6.92%) aligned exactly 1 time 612258 (9.69%) aligned >1 times 94.61% overall alignment rate Time searching: 00:47:47 Overall time: 00:47:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 24611919 / 45542418 = 0.5404 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:49:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:49:55: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:49:55: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:50:07: 1000000 INFO @ Thu, 09 Dec 2021 01:50:20: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:50:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:50:24: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:50:24: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:50:32: 3000000 INFO @ Thu, 09 Dec 2021 01:50:35: 1000000 INFO @ Thu, 09 Dec 2021 01:50:44: 4000000 INFO @ Thu, 09 Dec 2021 01:50:46: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:50:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:50:54: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:50:54: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:50:56: 5000000 INFO @ Thu, 09 Dec 2021 01:50:57: 3000000 INFO @ Thu, 09 Dec 2021 01:51:02: 1000000 INFO @ Thu, 09 Dec 2021 01:51:08: 4000000 INFO @ Thu, 09 Dec 2021 01:51:08: 6000000 INFO @ Thu, 09 Dec 2021 01:51:10: 2000000 INFO @ Thu, 09 Dec 2021 01:51:18: 3000000 INFO @ Thu, 09 Dec 2021 01:51:19: 5000000 INFO @ Thu, 09 Dec 2021 01:51:20: 7000000 INFO @ Thu, 09 Dec 2021 01:51:27: 4000000 INFO @ Thu, 09 Dec 2021 01:51:29: 6000000 INFO @ Thu, 09 Dec 2021 01:51:33: 8000000 INFO @ Thu, 09 Dec 2021 01:51:35: 5000000 INFO @ Thu, 09 Dec 2021 01:51:38: 7000000 INFO @ Thu, 09 Dec 2021 01:51:44: 6000000 INFO @ Thu, 09 Dec 2021 01:51:46: 9000000 INFO @ Thu, 09 Dec 2021 01:51:47: 8000000 INFO @ Thu, 09 Dec 2021 01:51:53: 7000000 INFO @ Thu, 09 Dec 2021 01:51:55: 9000000 INFO @ Thu, 09 Dec 2021 01:51:59: 10000000 INFO @ Thu, 09 Dec 2021 01:52:01: 8000000 INFO @ Thu, 09 Dec 2021 01:52:04: 10000000 INFO @ Thu, 09 Dec 2021 01:52:10: 9000000 INFO @ Thu, 09 Dec 2021 01:52:12: 11000000 INFO @ Thu, 09 Dec 2021 01:52:14: 11000000 INFO @ Thu, 09 Dec 2021 01:52:20: 10000000 INFO @ Thu, 09 Dec 2021 01:52:23: 12000000 INFO @ Thu, 09 Dec 2021 01:52:25: 12000000 INFO @ Thu, 09 Dec 2021 01:52:30: 11000000 INFO @ Thu, 09 Dec 2021 01:52:33: 13000000 INFO @ Thu, 09 Dec 2021 01:52:38: 13000000 INFO @ Thu, 09 Dec 2021 01:52:40: 12000000 INFO @ Thu, 09 Dec 2021 01:52:43: 14000000 INFO @ Thu, 09 Dec 2021 01:52:50: 13000000 INFO @ Thu, 09 Dec 2021 01:52:51: 14000000 INFO @ Thu, 09 Dec 2021 01:52:53: 15000000 INFO @ Thu, 09 Dec 2021 01:52:59: 14000000 INFO @ Thu, 09 Dec 2021 01:53:02: 16000000 INFO @ Thu, 09 Dec 2021 01:53:05: 15000000 INFO @ Thu, 09 Dec 2021 01:53:07: 15000000 INFO @ Thu, 09 Dec 2021 01:53:11: 17000000 INFO @ Thu, 09 Dec 2021 01:53:17: 16000000 INFO @ Thu, 09 Dec 2021 01:53:18: 16000000 INFO @ Thu, 09 Dec 2021 01:53:20: 18000000 INFO @ Thu, 09 Dec 2021 01:53:25: 17000000 INFO @ Thu, 09 Dec 2021 01:53:29: 19000000 INFO @ Thu, 09 Dec 2021 01:53:32: 17000000 INFO @ Thu, 09 Dec 2021 01:53:34: 18000000 INFO @ Thu, 09 Dec 2021 01:53:38: 20000000 INFO @ Thu, 09 Dec 2021 01:53:43: 19000000 INFO @ Thu, 09 Dec 2021 01:53:45: 18000000 INFO @ Thu, 09 Dec 2021 01:53:48: 21000000 INFO @ Thu, 09 Dec 2021 01:53:53: 20000000 INFO @ Thu, 09 Dec 2021 01:53:57: 22000000 INFO @ Thu, 09 Dec 2021 01:53:59: 19000000 INFO @ Thu, 09 Dec 2021 01:54:02: 21000000 INFO @ Thu, 09 Dec 2021 01:54:06: 23000000 INFO @ Thu, 09 Dec 2021 01:54:10: 22000000 INFO @ Thu, 09 Dec 2021 01:54:12: 20000000 INFO @ Thu, 09 Dec 2021 01:54:14: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 01:54:19: 23000000 INFO @ Thu, 09 Dec 2021 01:54:23: 25000000 INFO @ Thu, 09 Dec 2021 01:54:26: 21000000 INFO @ Thu, 09 Dec 2021 01:54:28: 24000000 INFO @ Thu, 09 Dec 2021 01:54:31: 26000000 INFO @ Thu, 09 Dec 2021 01:54:36: 25000000 INFO @ Thu, 09 Dec 2021 01:54:39: 22000000 INFO @ Thu, 09 Dec 2021 01:54:39: 27000000 INFO @ Thu, 09 Dec 2021 01:54:44: 26000000 INFO @ Thu, 09 Dec 2021 01:54:47: 28000000 INFO @ Thu, 09 Dec 2021 01:54:52: 27000000 INFO @ Thu, 09 Dec 2021 01:54:52: 23000000 INFO @ Thu, 09 Dec 2021 01:54:56: 29000000 INFO @ Thu, 09 Dec 2021 01:55:00: 28000000 INFO @ Thu, 09 Dec 2021 01:55:04: 30000000 INFO @ Thu, 09 Dec 2021 01:55:05: 24000000 INFO @ Thu, 09 Dec 2021 01:55:08: 29000000 INFO @ Thu, 09 Dec 2021 01:55:12: 31000000 INFO @ Thu, 09 Dec 2021 01:55:16: 30000000 INFO @ Thu, 09 Dec 2021 01:55:18: 25000000 BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 01:55:21: 32000000 INFO @ Thu, 09 Dec 2021 01:55:24: 31000000 INFO @ Thu, 09 Dec 2021 01:55:29: 33000000 INFO @ Thu, 09 Dec 2021 01:55:31: 26000000 INFO @ Thu, 09 Dec 2021 01:55:33: 32000000 INFO @ Thu, 09 Dec 2021 01:55:37: 34000000 INFO @ Thu, 09 Dec 2021 01:55:41: 33000000 INFO @ Thu, 09 Dec 2021 01:55:44: 27000000 INFO @ Thu, 09 Dec 2021 01:55:46: 35000000 INFO @ Thu, 09 Dec 2021 01:55:50: 34000000 INFO @ Thu, 09 Dec 2021 01:55:55: 36000000 INFO @ Thu, 09 Dec 2021 01:55:57: 28000000 INFO @ Thu, 09 Dec 2021 01:55:59: 35000000 INFO @ Thu, 09 Dec 2021 01:56:04: 37000000 INFO @ Thu, 09 Dec 2021 01:56:08: 36000000 INFO @ Thu, 09 Dec 2021 01:56:10: 29000000 INFO @ Thu, 09 Dec 2021 01:56:13: 38000000 INFO @ Thu, 09 Dec 2021 01:56:17: 37000000 INFO @ Thu, 09 Dec 2021 01:56:22: 39000000 INFO @ Thu, 09 Dec 2021 01:56:23: 30000000 INFO @ Thu, 09 Dec 2021 01:56:26: 38000000 INFO @ Thu, 09 Dec 2021 01:56:31: 40000000 INFO @ Thu, 09 Dec 2021 01:56:35: 39000000 INFO @ Thu, 09 Dec 2021 01:56:36: 31000000 INFO @ Thu, 09 Dec 2021 01:56:41: 41000000 INFO @ Thu, 09 Dec 2021 01:56:45: 40000000 INFO @ Thu, 09 Dec 2021 01:56:49: 32000000 INFO @ Thu, 09 Dec 2021 01:56:50: 42000000 INFO @ Thu, 09 Dec 2021 01:56:55: 41000000 INFO @ Thu, 09 Dec 2021 01:57:00: 43000000 INFO @ Thu, 09 Dec 2021 01:57:02: 33000000 INFO @ Thu, 09 Dec 2021 01:57:03: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:57:03: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:57:03: #1 total tags in treatment: 20464901 INFO @ Thu, 09 Dec 2021 01:57:03: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:57:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:57:03: #1 tags after filtering in treatment: 11523750 INFO @ Thu, 09 Dec 2021 01:57:03: #1 Redundant rate of treatment: 0.44 INFO @ Thu, 09 Dec 2021 01:57:03: #1 finished! INFO @ Thu, 09 Dec 2021 01:57:03: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:57:03: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:57:04: 42000000 INFO @ Thu, 09 Dec 2021 01:57:04: #2 number of paired peaks: 1083 INFO @ Thu, 09 Dec 2021 01:57:04: start model_add_line... INFO @ Thu, 09 Dec 2021 01:57:04: start X-correlation... INFO @ Thu, 09 Dec 2021 01:57:04: end of X-cor INFO @ Thu, 09 Dec 2021 01:57:04: #2 finished! INFO @ Thu, 09 Dec 2021 01:57:04: #2 predicted fragment length is 80 bps INFO @ Thu, 09 Dec 2021 01:57:04: #2 alternative fragment length(s) may be 80 bps INFO @ Thu, 09 Dec 2021 01:57:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.10_model.r WARNING @ Thu, 09 Dec 2021 01:57:04: #2 Since the d (80) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 01:57:04: #2 You may need to consider one of the other alternative d(s): 80 WARNING @ Thu, 09 Dec 2021 01:57:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 01:57:04: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:57:04: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:57:12: 43000000 INFO @ Thu, 09 Dec 2021 01:57:14: 34000000 INFO @ Thu, 09 Dec 2021 01:57:14: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:57:14: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:57:14: #1 total tags in treatment: 20464901 INFO @ Thu, 09 Dec 2021 01:57:14: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:57:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:57:15: #1 tags after filtering in treatment: 11523750 INFO @ Thu, 09 Dec 2021 01:57:15: #1 Redundant rate of treatment: 0.44 INFO @ Thu, 09 Dec 2021 01:57:15: #1 finished! INFO @ Thu, 09 Dec 2021 01:57:15: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:57:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:57:16: #2 number of paired peaks: 1083 INFO @ Thu, 09 Dec 2021 01:57:16: start model_add_line... INFO @ Thu, 09 Dec 2021 01:57:16: start X-correlation... INFO @ Thu, 09 Dec 2021 01:57:16: end of X-cor INFO @ Thu, 09 Dec 2021 01:57:16: #2 finished! INFO @ Thu, 09 Dec 2021 01:57:16: #2 predicted fragment length is 80 bps INFO @ Thu, 09 Dec 2021 01:57:16: #2 alternative fragment length(s) may be 80 bps INFO @ Thu, 09 Dec 2021 01:57:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.20_model.r WARNING @ Thu, 09 Dec 2021 01:57:16: #2 Since the d (80) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 01:57:16: #2 You may need to consider one of the other alternative d(s): 80 WARNING @ Thu, 09 Dec 2021 01:57:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 01:57:16: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:57:16: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:57:27: 35000000 INFO @ Thu, 09 Dec 2021 01:57:38: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:57:39: 36000000 INFO @ Thu, 09 Dec 2021 01:57:50: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:57:51: 37000000 INFO @ Thu, 09 Dec 2021 01:57:57: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.10_peaks.xls INFO @ Thu, 09 Dec 2021 01:57:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:57:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.10_summits.bed INFO @ Thu, 09 Dec 2021 01:57:57: Done! pass1 - making usageList (6 chroms): 3 millis pass2 - checking and writing primary data (8307 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:58:03: 38000000 INFO @ Thu, 09 Dec 2021 01:58:08: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.20_peaks.xls INFO @ Thu, 09 Dec 2021 01:58:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:58:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.20_summits.bed INFO @ Thu, 09 Dec 2021 01:58:08: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (4471 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:58:15: 39000000 INFO @ Thu, 09 Dec 2021 01:58:26: 40000000 INFO @ Thu, 09 Dec 2021 01:58:38: 41000000 INFO @ Thu, 09 Dec 2021 01:58:49: 42000000 INFO @ Thu, 09 Dec 2021 01:59:01: 43000000 INFO @ Thu, 09 Dec 2021 01:59:03: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:59:03: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:59:03: #1 total tags in treatment: 20464901 INFO @ Thu, 09 Dec 2021 01:59:03: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:59:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:59:04: #1 tags after filtering in treatment: 11523750 INFO @ Thu, 09 Dec 2021 01:59:04: #1 Redundant rate of treatment: 0.44 INFO @ Thu, 09 Dec 2021 01:59:04: #1 finished! INFO @ Thu, 09 Dec 2021 01:59:04: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:59:04: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:59:05: #2 number of paired peaks: 1083 INFO @ Thu, 09 Dec 2021 01:59:05: start model_add_line... INFO @ Thu, 09 Dec 2021 01:59:05: start X-correlation... INFO @ Thu, 09 Dec 2021 01:59:05: end of X-cor INFO @ Thu, 09 Dec 2021 01:59:05: #2 finished! INFO @ Thu, 09 Dec 2021 01:59:05: #2 predicted fragment length is 80 bps INFO @ Thu, 09 Dec 2021 01:59:05: #2 alternative fragment length(s) may be 80 bps INFO @ Thu, 09 Dec 2021 01:59:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.05_model.r WARNING @ Thu, 09 Dec 2021 01:59:05: #2 Since the d (80) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 01:59:05: #2 You may need to consider one of the other alternative d(s): 80 WARNING @ Thu, 09 Dec 2021 01:59:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 01:59:05: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:59:05: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:59:39: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:59:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.05_peaks.xls INFO @ Thu, 09 Dec 2021 01:59:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:59:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8845638/SRX8845638.05_summits.bed INFO @ Thu, 09 Dec 2021 01:59:58: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (11974 records, 4 fields): 16 millis CompletedMACS2peakCalling