Job ID = 14159771 SRX = SRX8845633 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:19 34679463 reads; of these: 34679463 (100.00%) were unpaired; of these: 562920 (1.62%) aligned 0 times 28280993 (81.55%) aligned exactly 1 time 5835550 (16.83%) aligned >1 times 98.38% overall alignment rate Time searching: 00:08:19 Overall time: 00:08:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4355730 / 34116543 = 0.1277 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 00:42:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 00:42:23: #1 read tag files... INFO @ Thu, 09 Dec 2021 00:42:23: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 00:42:30: 1000000 INFO @ Thu, 09 Dec 2021 00:42:36: 2000000 INFO @ Thu, 09 Dec 2021 00:42:42: 3000000 INFO @ Thu, 09 Dec 2021 00:42:49: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 00:42:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 00:42:53: #1 read tag files... INFO @ Thu, 09 Dec 2021 00:42:53: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 00:42:56: 5000000 INFO @ Thu, 09 Dec 2021 00:42:59: 1000000 INFO @ Thu, 09 Dec 2021 00:43:02: 6000000 INFO @ Thu, 09 Dec 2021 00:43:05: 2000000 INFO @ Thu, 09 Dec 2021 00:43:09: 7000000 INFO @ Thu, 09 Dec 2021 00:43:10: 3000000 INFO @ Thu, 09 Dec 2021 00:43:15: 8000000 INFO @ Thu, 09 Dec 2021 00:43:16: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 00:43:22: 9000000 INFO @ Thu, 09 Dec 2021 00:43:22: 5000000 INFO @ Thu, 09 Dec 2021 00:43:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 00:43:23: #1 read tag files... INFO @ Thu, 09 Dec 2021 00:43:23: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 00:43:28: 6000000 INFO @ Thu, 09 Dec 2021 00:43:29: 10000000 INFO @ Thu, 09 Dec 2021 00:43:30: 1000000 INFO @ Thu, 09 Dec 2021 00:43:34: 7000000 INFO @ Thu, 09 Dec 2021 00:43:36: 11000000 INFO @ Thu, 09 Dec 2021 00:43:37: 2000000 INFO @ Thu, 09 Dec 2021 00:43:40: 8000000 INFO @ Thu, 09 Dec 2021 00:43:43: 12000000 INFO @ Thu, 09 Dec 2021 00:43:44: 3000000 INFO @ Thu, 09 Dec 2021 00:43:46: 9000000 INFO @ Thu, 09 Dec 2021 00:43:50: 13000000 INFO @ Thu, 09 Dec 2021 00:43:51: 4000000 INFO @ Thu, 09 Dec 2021 00:43:52: 10000000 INFO @ Thu, 09 Dec 2021 00:43:56: 14000000 INFO @ Thu, 09 Dec 2021 00:43:58: 5000000 INFO @ Thu, 09 Dec 2021 00:43:58: 11000000 INFO @ Thu, 09 Dec 2021 00:44:03: 15000000 INFO @ Thu, 09 Dec 2021 00:44:04: 12000000 INFO @ Thu, 09 Dec 2021 00:44:04: 6000000 INFO @ Thu, 09 Dec 2021 00:44:10: 16000000 INFO @ Thu, 09 Dec 2021 00:44:10: 13000000 INFO @ Thu, 09 Dec 2021 00:44:11: 7000000 INFO @ Thu, 09 Dec 2021 00:44:16: 14000000 INFO @ Thu, 09 Dec 2021 00:44:17: 17000000 INFO @ Thu, 09 Dec 2021 00:44:18: 8000000 INFO @ Thu, 09 Dec 2021 00:44:22: 15000000 INFO @ Thu, 09 Dec 2021 00:44:24: 18000000 INFO @ Thu, 09 Dec 2021 00:44:25: 9000000 INFO @ Thu, 09 Dec 2021 00:44:28: 16000000 INFO @ Thu, 09 Dec 2021 00:44:30: 19000000 INFO @ Thu, 09 Dec 2021 00:44:32: 10000000 INFO @ Thu, 09 Dec 2021 00:44:34: 17000000 INFO @ Thu, 09 Dec 2021 00:44:37: 20000000 INFO @ Thu, 09 Dec 2021 00:44:38: 11000000 INFO @ Thu, 09 Dec 2021 00:44:40: 18000000 INFO @ Thu, 09 Dec 2021 00:44:44: 21000000 INFO @ Thu, 09 Dec 2021 00:44:45: 12000000 INFO @ Thu, 09 Dec 2021 00:44:46: 19000000 INFO @ Thu, 09 Dec 2021 00:44:51: 22000000 INFO @ Thu, 09 Dec 2021 00:44:52: 20000000 INFO @ Thu, 09 Dec 2021 00:44:52: 13000000 INFO @ Thu, 09 Dec 2021 00:44:58: 23000000 INFO @ Thu, 09 Dec 2021 00:44:58: 21000000 INFO @ Thu, 09 Dec 2021 00:44:59: 14000000 INFO @ Thu, 09 Dec 2021 00:45:04: 22000000 INFO @ Thu, 09 Dec 2021 00:45:05: 24000000 INFO @ Thu, 09 Dec 2021 00:45:06: 15000000 INFO @ Thu, 09 Dec 2021 00:45:10: 23000000 INFO @ Thu, 09 Dec 2021 00:45:11: 25000000 INFO @ Thu, 09 Dec 2021 00:45:13: 16000000 INFO @ Thu, 09 Dec 2021 00:45:16: 24000000 INFO @ Thu, 09 Dec 2021 00:45:18: 26000000 INFO @ Thu, 09 Dec 2021 00:45:20: 17000000 INFO @ Thu, 09 Dec 2021 00:45:22: 25000000 INFO @ Thu, 09 Dec 2021 00:45:25: 27000000 INFO @ Thu, 09 Dec 2021 00:45:27: 18000000 INFO @ Thu, 09 Dec 2021 00:45:27: 26000000 INFO @ Thu, 09 Dec 2021 00:45:31: 28000000 INFO @ Thu, 09 Dec 2021 00:45:33: 19000000 INFO @ Thu, 09 Dec 2021 00:45:33: 27000000 INFO @ Thu, 09 Dec 2021 00:45:38: 29000000 INFO @ Thu, 09 Dec 2021 00:45:39: 28000000 INFO @ Thu, 09 Dec 2021 00:45:40: 20000000 INFO @ Thu, 09 Dec 2021 00:45:43: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 00:45:43: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 00:45:43: #1 total tags in treatment: 29760813 INFO @ Thu, 09 Dec 2021 00:45:43: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 00:45:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 00:45:44: #1 tags after filtering in treatment: 29760813 INFO @ Thu, 09 Dec 2021 00:45:44: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 00:45:44: #1 finished! INFO @ Thu, 09 Dec 2021 00:45:44: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 00:45:44: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 00:45:45: 29000000 INFO @ Thu, 09 Dec 2021 00:45:46: #2 number of paired peaks: 92 WARNING @ Thu, 09 Dec 2021 00:45:46: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 00:45:46: Process for pairing-model is terminated! INFO @ Thu, 09 Dec 2021 00:45:46: 21000000 cut: /home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 00:45:49: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 00:45:49: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 00:45:49: #1 total tags in treatment: 29760813 INFO @ Thu, 09 Dec 2021 00:45:49: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 00:45:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 00:45:50: #1 tags after filtering in treatment: 29760813 INFO @ Thu, 09 Dec 2021 00:45:50: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 00:45:50: #1 finished! INFO @ Thu, 09 Dec 2021 00:45:50: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 00:45:50: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 00:45:52: #2 number of paired peaks: 92 WARNING @ Thu, 09 Dec 2021 00:45:52: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 00:45:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 00:45:53: 22000000 INFO @ Thu, 09 Dec 2021 00:45:59: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 00:46:06: 24000000 INFO @ Thu, 09 Dec 2021 00:46:12: 25000000 INFO @ Thu, 09 Dec 2021 00:46:18: 26000000 INFO @ Thu, 09 Dec 2021 00:46:24: 27000000 INFO @ Thu, 09 Dec 2021 00:46:31: 28000000 INFO @ Thu, 09 Dec 2021 00:46:37: 29000000 INFO @ Thu, 09 Dec 2021 00:46:42: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 00:46:42: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 00:46:42: #1 total tags in treatment: 29760813 INFO @ Thu, 09 Dec 2021 00:46:42: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 00:46:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 00:46:42: #1 tags after filtering in treatment: 29760813 INFO @ Thu, 09 Dec 2021 00:46:42: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 00:46:42: #1 finished! INFO @ Thu, 09 Dec 2021 00:46:42: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 00:46:42: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 00:46:44: #2 number of paired peaks: 92 WARNING @ Thu, 09 Dec 2021 00:46:44: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 00:46:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8845633/SRX8845633.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。