Job ID = 14160293 SRX = SRX8832112 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:08 17679744 reads; of these: 17679744 (100.00%) were unpaired; of these: 2758296 (15.60%) aligned 0 times 12428172 (70.30%) aligned exactly 1 time 2493276 (14.10%) aligned >1 times 84.40% overall alignment rate Time searching: 00:04:08 Overall time: 00:04:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3707154 / 14921448 = 0.2484 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:23:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:23:19: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:23:19: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:23:27: 1000000 INFO @ Thu, 09 Dec 2021 01:23:34: 2000000 INFO @ Thu, 09 Dec 2021 01:23:42: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:23:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:23:49: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:23:49: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:23:50: 4000000 INFO @ Thu, 09 Dec 2021 01:23:57: 5000000 INFO @ Thu, 09 Dec 2021 01:23:57: 1000000 INFO @ Thu, 09 Dec 2021 01:24:05: 6000000 INFO @ Thu, 09 Dec 2021 01:24:05: 2000000 INFO @ Thu, 09 Dec 2021 01:24:12: 7000000 INFO @ Thu, 09 Dec 2021 01:24:13: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:24:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:24:19: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:24:19: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:24:20: 8000000 INFO @ Thu, 09 Dec 2021 01:24:21: 4000000 INFO @ Thu, 09 Dec 2021 01:24:28: 9000000 INFO @ Thu, 09 Dec 2021 01:24:28: 1000000 INFO @ Thu, 09 Dec 2021 01:24:29: 5000000 INFO @ Thu, 09 Dec 2021 01:24:36: 10000000 INFO @ Thu, 09 Dec 2021 01:24:37: 2000000 INFO @ Thu, 09 Dec 2021 01:24:37: 6000000 INFO @ Thu, 09 Dec 2021 01:24:45: 11000000 INFO @ Thu, 09 Dec 2021 01:24:45: 3000000 INFO @ Thu, 09 Dec 2021 01:24:45: 7000000 INFO @ Thu, 09 Dec 2021 01:24:46: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:24:46: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:24:46: #1 total tags in treatment: 11214294 INFO @ Thu, 09 Dec 2021 01:24:46: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:24:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:24:47: #1 tags after filtering in treatment: 11214294 INFO @ Thu, 09 Dec 2021 01:24:47: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:24:47: #1 finished! INFO @ Thu, 09 Dec 2021 01:24:47: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:24:47: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:24:48: #2 number of paired peaks: 2184 INFO @ Thu, 09 Dec 2021 01:24:48: start model_add_line... INFO @ Thu, 09 Dec 2021 01:24:48: start X-correlation... INFO @ Thu, 09 Dec 2021 01:24:48: end of X-cor INFO @ Thu, 09 Dec 2021 01:24:48: #2 finished! INFO @ Thu, 09 Dec 2021 01:24:48: #2 predicted fragment length is 141 bps INFO @ Thu, 09 Dec 2021 01:24:48: #2 alternative fragment length(s) may be 141 bps INFO @ Thu, 09 Dec 2021 01:24:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.05_model.r INFO @ Thu, 09 Dec 2021 01:24:48: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:24:48: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:24:53: 8000000 INFO @ Thu, 09 Dec 2021 01:24:53: 4000000 INFO @ Thu, 09 Dec 2021 01:25:02: 9000000 INFO @ Thu, 09 Dec 2021 01:25:02: 5000000 INFO @ Thu, 09 Dec 2021 01:25:10: 10000000 INFO @ Thu, 09 Dec 2021 01:25:10: 6000000 INFO @ Thu, 09 Dec 2021 01:25:18: 11000000 INFO @ Thu, 09 Dec 2021 01:25:18: 7000000 INFO @ Thu, 09 Dec 2021 01:25:20: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:25:20: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:25:20: #1 total tags in treatment: 11214294 INFO @ Thu, 09 Dec 2021 01:25:20: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:25:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:25:20: #1 tags after filtering in treatment: 11214294 INFO @ Thu, 09 Dec 2021 01:25:20: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:25:20: #1 finished! INFO @ Thu, 09 Dec 2021 01:25:20: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:25:20: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:25:21: #2 number of paired peaks: 2184 INFO @ Thu, 09 Dec 2021 01:25:21: start model_add_line... INFO @ Thu, 09 Dec 2021 01:25:22: start X-correlation... INFO @ Thu, 09 Dec 2021 01:25:22: end of X-cor INFO @ Thu, 09 Dec 2021 01:25:22: #2 finished! INFO @ Thu, 09 Dec 2021 01:25:22: #2 predicted fragment length is 141 bps INFO @ Thu, 09 Dec 2021 01:25:22: #2 alternative fragment length(s) may be 141 bps INFO @ Thu, 09 Dec 2021 01:25:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.10_model.r INFO @ Thu, 09 Dec 2021 01:25:22: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:25:22: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:25:23: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:25:27: 8000000 INFO @ Thu, 09 Dec 2021 01:25:36: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 01:25:40: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.05_peaks.xls INFO @ Thu, 09 Dec 2021 01:25:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:25:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.05_summits.bed INFO @ Thu, 09 Dec 2021 01:25:40: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (8207 records, 4 fields): 55 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:25:45: 10000000 INFO @ Thu, 09 Dec 2021 01:25:54: 11000000 INFO @ Thu, 09 Dec 2021 01:25:55: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:25:55: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:25:55: #1 total tags in treatment: 11214294 INFO @ Thu, 09 Dec 2021 01:25:55: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:25:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:25:55: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:25:55: #1 tags after filtering in treatment: 11214294 INFO @ Thu, 09 Dec 2021 01:25:55: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:25:55: #1 finished! INFO @ Thu, 09 Dec 2021 01:25:55: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:25:55: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:25:57: #2 number of paired peaks: 2184 INFO @ Thu, 09 Dec 2021 01:25:57: start model_add_line... INFO @ Thu, 09 Dec 2021 01:25:57: start X-correlation... INFO @ Thu, 09 Dec 2021 01:25:57: end of X-cor INFO @ Thu, 09 Dec 2021 01:25:57: #2 finished! INFO @ Thu, 09 Dec 2021 01:25:57: #2 predicted fragment length is 141 bps INFO @ Thu, 09 Dec 2021 01:25:57: #2 alternative fragment length(s) may be 141 bps INFO @ Thu, 09 Dec 2021 01:25:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.20_model.r INFO @ Thu, 09 Dec 2021 01:25:57: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:25:57: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 01:26:12: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.10_peaks.xls INFO @ Thu, 09 Dec 2021 01:26:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:26:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.10_summits.bed INFO @ Thu, 09 Dec 2021 01:26:12: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (6554 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:26:31: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:26:47: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.20_peaks.xls INFO @ Thu, 09 Dec 2021 01:26:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:26:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832112/SRX8832112.20_summits.bed INFO @ Thu, 09 Dec 2021 01:26:47: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (4836 records, 4 fields): 11 millis CompletedMACS2peakCalling