Job ID = 14160229 SRX = SRX8832102 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:16 18464781 reads; of these: 18464781 (100.00%) were unpaired; of these: 2399684 (13.00%) aligned 0 times 13535377 (73.30%) aligned exactly 1 time 2529720 (13.70%) aligned >1 times 87.00% overall alignment rate Time searching: 00:04:16 Overall time: 00:04:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7974720 / 16065097 = 0.4964 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:11:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:11:12: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:11:12: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:11:20: 1000000 INFO @ Thu, 09 Dec 2021 01:11:27: 2000000 INFO @ Thu, 09 Dec 2021 01:11:35: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:11:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:11:40: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:11:40: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:11:43: 4000000 INFO @ Thu, 09 Dec 2021 01:11:51: 1000000 INFO @ Thu, 09 Dec 2021 01:11:52: 5000000 INFO @ Thu, 09 Dec 2021 01:12:00: 6000000 INFO @ Thu, 09 Dec 2021 01:12:02: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:12:09: 7000000 INFO @ Thu, 09 Dec 2021 01:12:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:12:10: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:12:10: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:12:13: 3000000 INFO @ Thu, 09 Dec 2021 01:12:17: 8000000 INFO @ Thu, 09 Dec 2021 01:12:18: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:12:18: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:12:18: #1 total tags in treatment: 8090377 INFO @ Thu, 09 Dec 2021 01:12:18: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:12:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:12:18: #1 tags after filtering in treatment: 8090377 INFO @ Thu, 09 Dec 2021 01:12:18: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:12:18: #1 finished! INFO @ Thu, 09 Dec 2021 01:12:18: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:12:18: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:12:19: 1000000 INFO @ Thu, 09 Dec 2021 01:12:20: #2 number of paired peaks: 5495 INFO @ Thu, 09 Dec 2021 01:12:20: start model_add_line... INFO @ Thu, 09 Dec 2021 01:12:20: start X-correlation... INFO @ Thu, 09 Dec 2021 01:12:20: end of X-cor INFO @ Thu, 09 Dec 2021 01:12:20: #2 finished! INFO @ Thu, 09 Dec 2021 01:12:20: #2 predicted fragment length is 164 bps INFO @ Thu, 09 Dec 2021 01:12:20: #2 alternative fragment length(s) may be 164 bps INFO @ Thu, 09 Dec 2021 01:12:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.05_model.r INFO @ Thu, 09 Dec 2021 01:12:20: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:12:20: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:12:23: 4000000 INFO @ Thu, 09 Dec 2021 01:12:27: 2000000 INFO @ Thu, 09 Dec 2021 01:12:34: 5000000 INFO @ Thu, 09 Dec 2021 01:12:36: 3000000 INFO @ Thu, 09 Dec 2021 01:12:44: 4000000 INFO @ Thu, 09 Dec 2021 01:12:45: 6000000 INFO @ Thu, 09 Dec 2021 01:12:49: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:12:53: 5000000 INFO @ Thu, 09 Dec 2021 01:12:55: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 01:13:01: 6000000 INFO @ Thu, 09 Dec 2021 01:13:02: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.05_peaks.xls INFO @ Thu, 09 Dec 2021 01:13:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:13:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.05_summits.bed INFO @ Thu, 09 Dec 2021 01:13:02: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (8692 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:13:06: 8000000 INFO @ Thu, 09 Dec 2021 01:13:07: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:13:07: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:13:07: #1 total tags in treatment: 8090377 INFO @ Thu, 09 Dec 2021 01:13:07: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:13:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:13:07: #1 tags after filtering in treatment: 8090377 INFO @ Thu, 09 Dec 2021 01:13:07: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:13:07: #1 finished! INFO @ Thu, 09 Dec 2021 01:13:07: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:13:07: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:13:08: #2 number of paired peaks: 5495 INFO @ Thu, 09 Dec 2021 01:13:08: start model_add_line... INFO @ Thu, 09 Dec 2021 01:13:08: start X-correlation... INFO @ Thu, 09 Dec 2021 01:13:08: end of X-cor INFO @ Thu, 09 Dec 2021 01:13:08: #2 finished! INFO @ Thu, 09 Dec 2021 01:13:08: #2 predicted fragment length is 164 bps INFO @ Thu, 09 Dec 2021 01:13:08: #2 alternative fragment length(s) may be 164 bps INFO @ Thu, 09 Dec 2021 01:13:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.10_model.r INFO @ Thu, 09 Dec 2021 01:13:08: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:13:08: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:13:09: 7000000 BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 01:13:17: 8000000 INFO @ Thu, 09 Dec 2021 01:13:18: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:13:18: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:13:18: #1 total tags in treatment: 8090377 INFO @ Thu, 09 Dec 2021 01:13:18: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:13:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:13:18: #1 tags after filtering in treatment: 8090377 INFO @ Thu, 09 Dec 2021 01:13:18: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:13:18: #1 finished! INFO @ Thu, 09 Dec 2021 01:13:18: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:13:18: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:13:20: #2 number of paired peaks: 5495 INFO @ Thu, 09 Dec 2021 01:13:20: start model_add_line... INFO @ Thu, 09 Dec 2021 01:13:20: start X-correlation... INFO @ Thu, 09 Dec 2021 01:13:20: end of X-cor INFO @ Thu, 09 Dec 2021 01:13:20: #2 finished! INFO @ Thu, 09 Dec 2021 01:13:20: #2 predicted fragment length is 164 bps INFO @ Thu, 09 Dec 2021 01:13:20: #2 alternative fragment length(s) may be 164 bps INFO @ Thu, 09 Dec 2021 01:13:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.20_model.r INFO @ Thu, 09 Dec 2021 01:13:20: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:13:20: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:13:37: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:13:48: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:13:49: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.10_peaks.xls INFO @ Thu, 09 Dec 2021 01:13:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:13:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.10_summits.bed INFO @ Thu, 09 Dec 2021 01:13:49: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (7091 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:14:01: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.20_peaks.xls INFO @ Thu, 09 Dec 2021 01:14:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:14:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832102/SRX8832102.20_summits.bed INFO @ Thu, 09 Dec 2021 01:14:01: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (5527 records, 4 fields): 11 millis CompletedMACS2peakCalling