Job ID = 14160424 SRX = SRX8832099 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:53 17513053 reads; of these: 17513053 (100.00%) were unpaired; of these: 932294 (5.32%) aligned 0 times 14438183 (82.44%) aligned exactly 1 time 2142576 (12.23%) aligned >1 times 94.68% overall alignment rate Time searching: 00:03:53 Overall time: 00:03:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8817270 / 16580759 = 0.5318 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:30:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:30:24: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:30:24: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:30:33: 1000000 INFO @ Thu, 09 Dec 2021 02:30:42: 2000000 INFO @ Thu, 09 Dec 2021 02:30:51: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:30:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:30:54: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:30:54: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:31:00: 4000000 INFO @ Thu, 09 Dec 2021 02:31:03: 1000000 INFO @ Thu, 09 Dec 2021 02:31:09: 5000000 INFO @ Thu, 09 Dec 2021 02:31:12: 2000000 INFO @ Thu, 09 Dec 2021 02:31:18: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:31:21: 3000000 INFO @ Thu, 09 Dec 2021 02:31:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:31:24: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:31:24: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:31:27: 7000000 INFO @ Thu, 09 Dec 2021 02:31:30: 4000000 INFO @ Thu, 09 Dec 2021 02:31:31: 1000000 INFO @ Thu, 09 Dec 2021 02:31:34: #1 tag size is determined as 49 bps INFO @ Thu, 09 Dec 2021 02:31:34: #1 tag size = 49 INFO @ Thu, 09 Dec 2021 02:31:34: #1 total tags in treatment: 7763489 INFO @ Thu, 09 Dec 2021 02:31:34: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:31:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:31:34: #1 tags after filtering in treatment: 7763489 INFO @ Thu, 09 Dec 2021 02:31:34: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:31:34: #1 finished! INFO @ Thu, 09 Dec 2021 02:31:34: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:31:34: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:31:35: #2 number of paired peaks: 4432 INFO @ Thu, 09 Dec 2021 02:31:35: start model_add_line... INFO @ Thu, 09 Dec 2021 02:31:35: start X-correlation... INFO @ Thu, 09 Dec 2021 02:31:35: end of X-cor INFO @ Thu, 09 Dec 2021 02:31:35: #2 finished! INFO @ Thu, 09 Dec 2021 02:31:35: #2 predicted fragment length is 152 bps INFO @ Thu, 09 Dec 2021 02:31:35: #2 alternative fragment length(s) may be 152 bps INFO @ Thu, 09 Dec 2021 02:31:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.05_model.r INFO @ Thu, 09 Dec 2021 02:31:35: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:31:35: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 02:31:37: 2000000 INFO @ Thu, 09 Dec 2021 02:31:39: 5000000 INFO @ Thu, 09 Dec 2021 02:31:44: 3000000 INFO @ Thu, 09 Dec 2021 02:31:48: 6000000 INFO @ Thu, 09 Dec 2021 02:31:50: 4000000 INFO @ Thu, 09 Dec 2021 02:31:56: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 02:31:57: 5000000 INFO @ Thu, 09 Dec 2021 02:31:57: 7000000 INFO @ Thu, 09 Dec 2021 02:32:04: 6000000 INFO @ Thu, 09 Dec 2021 02:32:04: #1 tag size is determined as 49 bps INFO @ Thu, 09 Dec 2021 02:32:04: #1 tag size = 49 INFO @ Thu, 09 Dec 2021 02:32:04: #1 total tags in treatment: 7763489 INFO @ Thu, 09 Dec 2021 02:32:04: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:32:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:32:04: #1 tags after filtering in treatment: 7763489 INFO @ Thu, 09 Dec 2021 02:32:04: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:32:04: #1 finished! INFO @ Thu, 09 Dec 2021 02:32:04: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:32:04: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:32:05: #2 number of paired peaks: 4432 INFO @ Thu, 09 Dec 2021 02:32:05: start model_add_line... INFO @ Thu, 09 Dec 2021 02:32:05: start X-correlation... INFO @ Thu, 09 Dec 2021 02:32:05: end of X-cor INFO @ Thu, 09 Dec 2021 02:32:05: #2 finished! INFO @ Thu, 09 Dec 2021 02:32:05: #2 predicted fragment length is 152 bps INFO @ Thu, 09 Dec 2021 02:32:05: #2 alternative fragment length(s) may be 152 bps INFO @ Thu, 09 Dec 2021 02:32:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.10_model.r INFO @ Thu, 09 Dec 2021 02:32:05: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:32:05: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 02:32:07: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.05_peaks.xls INFO @ Thu, 09 Dec 2021 02:32:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:32:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.05_summits.bed INFO @ Thu, 09 Dec 2021 02:32:07: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (7671 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:32:10: 7000000 INFO @ Thu, 09 Dec 2021 02:32:15: #1 tag size is determined as 49 bps INFO @ Thu, 09 Dec 2021 02:32:15: #1 tag size = 49 INFO @ Thu, 09 Dec 2021 02:32:15: #1 total tags in treatment: 7763489 INFO @ Thu, 09 Dec 2021 02:32:15: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:32:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:32:15: #1 tags after filtering in treatment: 7763489 INFO @ Thu, 09 Dec 2021 02:32:15: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:32:15: #1 finished! INFO @ Thu, 09 Dec 2021 02:32:15: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:32:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:32:16: #2 number of paired peaks: 4432 INFO @ Thu, 09 Dec 2021 02:32:16: start model_add_line... INFO @ Thu, 09 Dec 2021 02:32:16: start X-correlation... INFO @ Thu, 09 Dec 2021 02:32:16: end of X-cor INFO @ Thu, 09 Dec 2021 02:32:16: #2 finished! INFO @ Thu, 09 Dec 2021 02:32:16: #2 predicted fragment length is 152 bps INFO @ Thu, 09 Dec 2021 02:32:16: #2 alternative fragment length(s) may be 152 bps INFO @ Thu, 09 Dec 2021 02:32:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.20_model.r INFO @ Thu, 09 Dec 2021 02:32:16: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:32:16: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 02:32:26: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 02:32:37: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 02:32:37: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.10_peaks.xls INFO @ Thu, 09 Dec 2021 02:32:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:32:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.10_summits.bed INFO @ Thu, 09 Dec 2021 02:32:37: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (6294 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:32:46: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.20_peaks.xls INFO @ Thu, 09 Dec 2021 02:32:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:32:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832099/SRX8832099.20_summits.bed INFO @ Thu, 09 Dec 2021 02:32:46: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (4881 records, 4 fields): 8 millis CompletedMACS2peakCalling