Job ID = 10165980 SRX = SRX8832094 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:02:52 15511694 reads; of these: 15511694 (100.00%) were unpaired; of these: 4057277 (26.16%) aligned 0 times 9519892 (61.37%) aligned exactly 1 time 1934525 (12.47%) aligned >1 times 73.84% overall alignment rate Time searching: 00:02:53 Overall time: 00:02:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2583419 / 11454417 = 0.2255 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:25:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:25:47: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:25:47: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:25:59: 1000000 INFO @ Thu, 08 Oct 2020 20:26:10: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:26:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:26:17: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:26:17: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:26:21: 3000000 INFO @ Thu, 08 Oct 2020 20:26:28: 1000000 INFO @ Thu, 08 Oct 2020 20:26:31: 4000000 INFO @ Thu, 08 Oct 2020 20:26:37: 2000000 INFO @ Thu, 08 Oct 2020 20:26:40: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:26:47: 3000000 INFO @ Thu, 08 Oct 2020 20:26:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:26:47: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:26:47: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:26:50: 6000000 INFO @ Thu, 08 Oct 2020 20:26:53: 1000000 INFO @ Thu, 08 Oct 2020 20:26:55: 4000000 INFO @ Thu, 08 Oct 2020 20:26:57: 7000000 INFO @ Thu, 08 Oct 2020 20:26:58: 2000000 INFO @ Thu, 08 Oct 2020 20:27:02: 5000000 INFO @ Thu, 08 Oct 2020 20:27:04: 3000000 INFO @ Thu, 08 Oct 2020 20:27:04: 8000000 INFO @ Thu, 08 Oct 2020 20:27:08: 6000000 INFO @ Thu, 08 Oct 2020 20:27:09: 4000000 INFO @ Thu, 08 Oct 2020 20:27:10: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:27:10: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:27:10: #1 total tags in treatment: 8870998 INFO @ Thu, 08 Oct 2020 20:27:10: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:27:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:27:10: #1 tags after filtering in treatment: 8870998 INFO @ Thu, 08 Oct 2020 20:27:10: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:27:10: #1 finished! INFO @ Thu, 08 Oct 2020 20:27:10: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:27:10: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:27:11: #2 number of paired peaks: 1146 INFO @ Thu, 08 Oct 2020 20:27:11: start model_add_line... INFO @ Thu, 08 Oct 2020 20:27:11: start X-correlation... INFO @ Thu, 08 Oct 2020 20:27:11: end of X-cor INFO @ Thu, 08 Oct 2020 20:27:11: #2 finished! INFO @ Thu, 08 Oct 2020 20:27:11: #2 predicted fragment length is 145 bps INFO @ Thu, 08 Oct 2020 20:27:11: #2 alternative fragment length(s) may be 145 bps INFO @ Thu, 08 Oct 2020 20:27:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.05_model.r INFO @ Thu, 08 Oct 2020 20:27:11: #3 Call peaks... INFO @ Thu, 08 Oct 2020 20:27:11: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 08 Oct 2020 20:27:15: 5000000 INFO @ Thu, 08 Oct 2020 20:27:15: 7000000 INFO @ Thu, 08 Oct 2020 20:27:21: 6000000 INFO @ Thu, 08 Oct 2020 20:27:22: 8000000 INFO @ Thu, 08 Oct 2020 20:27:26: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:27:28: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:27:28: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:27:28: #1 total tags in treatment: 8870998 INFO @ Thu, 08 Oct 2020 20:27:28: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:27:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:27:28: #1 tags after filtering in treatment: 8870998 INFO @ Thu, 08 Oct 2020 20:27:28: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:27:28: #1 finished! INFO @ Thu, 08 Oct 2020 20:27:28: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:27:28: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:27:29: #2 number of paired peaks: 1146 INFO @ Thu, 08 Oct 2020 20:27:29: start model_add_line... INFO @ Thu, 08 Oct 2020 20:27:29: start X-correlation... INFO @ Thu, 08 Oct 2020 20:27:29: end of X-cor INFO @ Thu, 08 Oct 2020 20:27:29: #2 finished! INFO @ Thu, 08 Oct 2020 20:27:29: #2 predicted fragment length is 145 bps INFO @ Thu, 08 Oct 2020 20:27:29: #2 alternative fragment length(s) may be 145 bps INFO @ Thu, 08 Oct 2020 20:27:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.10_model.r INFO @ Thu, 08 Oct 2020 20:27:29: #3 Call peaks... INFO @ Thu, 08 Oct 2020 20:27:29: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 08 Oct 2020 20:27:32: 8000000 INFO @ Thu, 08 Oct 2020 20:27:32: #3 Call peaks for each chromosome... INFO @ Thu, 08 Oct 2020 20:27:36: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:27:36: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:27:36: #1 total tags in treatment: 8870998 INFO @ Thu, 08 Oct 2020 20:27:36: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:27:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:27:36: #1 tags after filtering in treatment: 8870998 INFO @ Thu, 08 Oct 2020 20:27:36: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:27:36: #1 finished! INFO @ Thu, 08 Oct 2020 20:27:36: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:27:36: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:27:37: #2 number of paired peaks: 1146 INFO @ Thu, 08 Oct 2020 20:27:37: start model_add_line... INFO @ Thu, 08 Oct 2020 20:27:37: start X-correlation... INFO @ Thu, 08 Oct 2020 20:27:37: end of X-cor INFO @ Thu, 08 Oct 2020 20:27:37: #2 finished! INFO @ Thu, 08 Oct 2020 20:27:37: #2 predicted fragment length is 145 bps INFO @ Thu, 08 Oct 2020 20:27:37: #2 alternative fragment length(s) may be 145 bps INFO @ Thu, 08 Oct 2020 20:27:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.20_model.r INFO @ Thu, 08 Oct 2020 20:27:37: #3 Call peaks... INFO @ Thu, 08 Oct 2020 20:27:37: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 08 Oct 2020 20:27:43: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.05_peaks.xls INFO @ Thu, 08 Oct 2020 20:27:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.05_peaks.narrowPeak INFO @ Thu, 08 Oct 2020 20:27:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.05_summits.bed INFO @ Thu, 08 Oct 2020 20:27:43: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (5089 records, 4 fields): 6 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 20:27:50: #3 Call peaks for each chromosome... INFO @ Thu, 08 Oct 2020 20:27:57: #3 Call peaks for each chromosome... INFO @ Thu, 08 Oct 2020 20:28:02: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.10_peaks.xls INFO @ Thu, 08 Oct 2020 20:28:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.10_peaks.narrowPeak INFO @ Thu, 08 Oct 2020 20:28:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.10_summits.bed INFO @ Thu, 08 Oct 2020 20:28:02: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (3885 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:28:06: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.20_peaks.xls INFO @ Thu, 08 Oct 2020 20:28:06: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.20_peaks.narrowPeak INFO @ Thu, 08 Oct 2020 20:28:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832094/SRX8832094.20_summits.bed INFO @ Thu, 08 Oct 2020 20:28:06: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2587 records, 4 fields): 4 millis CompletedMACS2peakCalling