Job ID = 14160393 SRX = SRX8832090 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:04 19789477 reads; of these: 19789477 (100.00%) were unpaired; of these: 1345450 (6.80%) aligned 0 times 16180191 (81.76%) aligned exactly 1 time 2263836 (11.44%) aligned >1 times 93.20% overall alignment rate Time searching: 00:04:04 Overall time: 00:04:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 10695543 / 18444027 = 0.5799 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:12:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:12:34: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:12:34: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:12:39: 1000000 INFO @ Thu, 09 Dec 2021 02:12:44: 2000000 INFO @ Thu, 09 Dec 2021 02:12:49: 3000000 INFO @ Thu, 09 Dec 2021 02:12:54: 4000000 INFO @ Thu, 09 Dec 2021 02:12:59: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:13:04: 6000000 INFO @ Thu, 09 Dec 2021 02:13:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:13:04: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:13:04: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:13:09: 7000000 INFO @ Thu, 09 Dec 2021 02:13:10: 1000000 INFO @ Thu, 09 Dec 2021 02:13:13: #1 tag size is determined as 49 bps INFO @ Thu, 09 Dec 2021 02:13:13: #1 tag size = 49 INFO @ Thu, 09 Dec 2021 02:13:13: #1 total tags in treatment: 7748484 INFO @ Thu, 09 Dec 2021 02:13:13: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:13:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:13:13: #1 tags after filtering in treatment: 7748484 INFO @ Thu, 09 Dec 2021 02:13:13: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:13:13: #1 finished! INFO @ Thu, 09 Dec 2021 02:13:13: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:13:13: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:13:14: #2 number of paired peaks: 4974 INFO @ Thu, 09 Dec 2021 02:13:14: start model_add_line... INFO @ Thu, 09 Dec 2021 02:13:14: start X-correlation... INFO @ Thu, 09 Dec 2021 02:13:14: end of X-cor INFO @ Thu, 09 Dec 2021 02:13:14: #2 finished! INFO @ Thu, 09 Dec 2021 02:13:14: #2 predicted fragment length is 144 bps INFO @ Thu, 09 Dec 2021 02:13:14: #2 alternative fragment length(s) may be 144 bps INFO @ Thu, 09 Dec 2021 02:13:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.05_model.r INFO @ Thu, 09 Dec 2021 02:13:14: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:13:14: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 02:13:15: 2000000 INFO @ Thu, 09 Dec 2021 02:13:20: 3000000 INFO @ Thu, 09 Dec 2021 02:13:25: 4000000 INFO @ Thu, 09 Dec 2021 02:13:30: 5000000 INFO @ Thu, 09 Dec 2021 02:13:32: #3 Call peaks for each chromosome... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:13:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:13:34: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:13:34: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:13:35: 6000000 INFO @ Thu, 09 Dec 2021 02:13:40: 7000000 INFO @ Thu, 09 Dec 2021 02:13:40: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.05_peaks.xls INFO @ Thu, 09 Dec 2021 02:13:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:13:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.05_summits.bed INFO @ Thu, 09 Dec 2021 02:13:41: Done! pass1 - making usageList (7 chroms): 2 millis INFO @ Thu, 09 Dec 2021 02:13:41: 1000000 pass2 - checking and writing primary data (8246 records, 4 fields): 28 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:13:45: #1 tag size is determined as 49 bps INFO @ Thu, 09 Dec 2021 02:13:45: #1 tag size = 49 INFO @ Thu, 09 Dec 2021 02:13:45: #1 total tags in treatment: 7748484 INFO @ Thu, 09 Dec 2021 02:13:45: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:13:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:13:45: #1 tags after filtering in treatment: 7748484 INFO @ Thu, 09 Dec 2021 02:13:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:13:45: #1 finished! INFO @ Thu, 09 Dec 2021 02:13:45: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:13:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:13:46: #2 number of paired peaks: 4974 INFO @ Thu, 09 Dec 2021 02:13:46: start model_add_line... INFO @ Thu, 09 Dec 2021 02:13:46: start X-correlation... INFO @ Thu, 09 Dec 2021 02:13:46: end of X-cor INFO @ Thu, 09 Dec 2021 02:13:46: #2 finished! INFO @ Thu, 09 Dec 2021 02:13:46: #2 predicted fragment length is 144 bps INFO @ Thu, 09 Dec 2021 02:13:46: #2 alternative fragment length(s) may be 144 bps INFO @ Thu, 09 Dec 2021 02:13:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.10_model.r INFO @ Thu, 09 Dec 2021 02:13:46: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:13:46: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 02:13:47: 2000000 INFO @ Thu, 09 Dec 2021 02:13:53: 3000000 INFO @ Thu, 09 Dec 2021 02:13:59: 4000000 INFO @ Thu, 09 Dec 2021 02:14:04: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 02:14:04: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 02:14:10: 6000000 INFO @ Thu, 09 Dec 2021 02:14:12: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.10_peaks.xls INFO @ Thu, 09 Dec 2021 02:14:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:14:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.10_summits.bed INFO @ Thu, 09 Dec 2021 02:14:12: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (7011 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:14:16: 7000000 BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 02:14:21: #1 tag size is determined as 49 bps INFO @ Thu, 09 Dec 2021 02:14:21: #1 tag size = 49 INFO @ Thu, 09 Dec 2021 02:14:21: #1 total tags in treatment: 7748484 INFO @ Thu, 09 Dec 2021 02:14:21: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:14:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:14:21: #1 tags after filtering in treatment: 7748484 INFO @ Thu, 09 Dec 2021 02:14:21: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:14:21: #1 finished! INFO @ Thu, 09 Dec 2021 02:14:21: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:14:21: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:14:22: #2 number of paired peaks: 4974 INFO @ Thu, 09 Dec 2021 02:14:22: start model_add_line... INFO @ Thu, 09 Dec 2021 02:14:22: start X-correlation... INFO @ Thu, 09 Dec 2021 02:14:22: end of X-cor INFO @ Thu, 09 Dec 2021 02:14:22: #2 finished! INFO @ Thu, 09 Dec 2021 02:14:22: #2 predicted fragment length is 144 bps INFO @ Thu, 09 Dec 2021 02:14:22: #2 alternative fragment length(s) may be 144 bps INFO @ Thu, 09 Dec 2021 02:14:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.20_model.r INFO @ Thu, 09 Dec 2021 02:14:22: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:14:22: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 02:14:40: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 02:14:48: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.20_peaks.xls INFO @ Thu, 09 Dec 2021 02:14:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:14:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832090/SRX8832090.20_summits.bed INFO @ Thu, 09 Dec 2021 02:14:48: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (5587 records, 4 fields): 7 millis CompletedMACS2peakCalling