Job ID = 10165855 SRX = SRX8832068 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:03:20 15364166 reads; of these: 15364166 (100.00%) were unpaired; of these: 750604 (4.89%) aligned 0 times 12721219 (82.80%) aligned exactly 1 time 1892343 (12.32%) aligned >1 times 95.11% overall alignment rate Time searching: 00:03:21 Overall time: 00:03:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2119214 / 14613562 = 0.1450 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:06:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:06:57: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:06:57: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:07:02: 1000000 INFO @ Thu, 08 Oct 2020 20:07:07: 2000000 INFO @ Thu, 08 Oct 2020 20:07:12: 3000000 INFO @ Thu, 08 Oct 2020 20:07:17: 4000000 INFO @ Thu, 08 Oct 2020 20:07:23: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:07:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:07:27: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:07:27: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:07:28: 6000000 INFO @ Thu, 08 Oct 2020 20:07:32: 1000000 INFO @ Thu, 08 Oct 2020 20:07:33: 7000000 INFO @ Thu, 08 Oct 2020 20:07:37: 2000000 INFO @ Thu, 08 Oct 2020 20:07:38: 8000000 INFO @ Thu, 08 Oct 2020 20:07:43: 3000000 INFO @ Thu, 08 Oct 2020 20:07:44: 9000000 INFO @ Thu, 08 Oct 2020 20:07:48: 4000000 INFO @ Thu, 08 Oct 2020 20:07:49: 10000000 INFO @ Thu, 08 Oct 2020 20:07:53: 5000000 INFO @ Thu, 08 Oct 2020 20:07:54: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:07:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:07:57: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:07:57: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:07:59: 6000000 INFO @ Thu, 08 Oct 2020 20:08:00: 12000000 INFO @ Thu, 08 Oct 2020 20:08:02: 1000000 INFO @ Thu, 08 Oct 2020 20:08:02: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:08:02: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:08:02: #1 total tags in treatment: 12494348 INFO @ Thu, 08 Oct 2020 20:08:02: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:08:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:08:03: #1 tags after filtering in treatment: 12494348 INFO @ Thu, 08 Oct 2020 20:08:03: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:08:03: #1 finished! INFO @ Thu, 08 Oct 2020 20:08:03: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:08:03: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:08:04: #2 number of paired peaks: 3278 INFO @ Thu, 08 Oct 2020 20:08:04: start model_add_line... INFO @ Thu, 08 Oct 2020 20:08:04: start X-correlation... INFO @ Thu, 08 Oct 2020 20:08:04: end of X-cor INFO @ Thu, 08 Oct 2020 20:08:04: #2 finished! INFO @ Thu, 08 Oct 2020 20:08:04: #2 predicted fragment length is 154 bps INFO @ Thu, 08 Oct 2020 20:08:04: #2 alternative fragment length(s) may be 4,154 bps INFO @ Thu, 08 Oct 2020 20:08:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.05_model.r INFO @ Thu, 08 Oct 2020 20:08:04: #3 Call peaks... INFO @ Thu, 08 Oct 2020 20:08:04: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 08 Oct 2020 20:08:04: 7000000 INFO @ Thu, 08 Oct 2020 20:08:08: 2000000 INFO @ Thu, 08 Oct 2020 20:08:09: 8000000 INFO @ Thu, 08 Oct 2020 20:08:13: 3000000 INFO @ Thu, 08 Oct 2020 20:08:15: 9000000 INFO @ Thu, 08 Oct 2020 20:08:18: 4000000 INFO @ Thu, 08 Oct 2020 20:08:20: 10000000 INFO @ Thu, 08 Oct 2020 20:08:24: 5000000 INFO @ Thu, 08 Oct 2020 20:08:26: 11000000 INFO @ Thu, 08 Oct 2020 20:08:29: 6000000 INFO @ Thu, 08 Oct 2020 20:08:31: 12000000 INFO @ Thu, 08 Oct 2020 20:08:34: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:08:34: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:08:34: #1 total tags in treatment: 12494348 INFO @ Thu, 08 Oct 2020 20:08:34: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:08:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:08:34: #1 tags after filtering in treatment: 12494348 INFO @ Thu, 08 Oct 2020 20:08:34: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:08:34: #1 finished! INFO @ Thu, 08 Oct 2020 20:08:34: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:08:34: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:08:34: 7000000 INFO @ Thu, 08 Oct 2020 20:08:35: #2 number of paired peaks: 3278 INFO @ Thu, 08 Oct 2020 20:08:35: start model_add_line... INFO @ Thu, 08 Oct 2020 20:08:35: start X-correlation... INFO @ Thu, 08 Oct 2020 20:08:35: end of X-cor INFO @ Thu, 08 Oct 2020 20:08:35: #2 finished! INFO @ Thu, 08 Oct 2020 20:08:35: #2 predicted fragment length is 154 bps INFO @ Thu, 08 Oct 2020 20:08:35: #2 alternative fragment length(s) may be 4,154 bps INFO @ Thu, 08 Oct 2020 20:08:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.10_model.r INFO @ Thu, 08 Oct 2020 20:08:35: #3 Call peaks... INFO @ Thu, 08 Oct 2020 20:08:35: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 08 Oct 2020 20:08:37: #3 Call peaks for each chromosome... INFO @ Thu, 08 Oct 2020 20:08:39: 8000000 INFO @ Thu, 08 Oct 2020 20:08:45: 9000000 INFO @ Thu, 08 Oct 2020 20:08:50: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:08:54: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.05_peaks.xls INFO @ Thu, 08 Oct 2020 20:08:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.05_peaks.narrowPeak INFO @ Thu, 08 Oct 2020 20:08:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.05_summits.bed INFO @ Thu, 08 Oct 2020 20:08:54: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (7726 records, 4 fields): 41 millis CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:08:55: 11000000 INFO @ Thu, 08 Oct 2020 20:09:00: 12000000 INFO @ Thu, 08 Oct 2020 20:09:03: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:09:03: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:09:03: #1 total tags in treatment: 12494348 INFO @ Thu, 08 Oct 2020 20:09:03: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:09:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:09:03: #1 tags after filtering in treatment: 12494348 INFO @ Thu, 08 Oct 2020 20:09:03: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:09:03: #1 finished! INFO @ Thu, 08 Oct 2020 20:09:03: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:09:03: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:09:04: #2 number of paired peaks: 3278 INFO @ Thu, 08 Oct 2020 20:09:04: start model_add_line... INFO @ Thu, 08 Oct 2020 20:09:04: start X-correlation... INFO @ Thu, 08 Oct 2020 20:09:04: end of X-cor INFO @ Thu, 08 Oct 2020 20:09:04: #2 finished! INFO @ Thu, 08 Oct 2020 20:09:04: #2 predicted fragment length is 154 bps INFO @ Thu, 08 Oct 2020 20:09:04: #2 alternative fragment length(s) may be 4,154 bps INFO @ Thu, 08 Oct 2020 20:09:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.20_model.r INFO @ Thu, 08 Oct 2020 20:09:04: #3 Call peaks... INFO @ Thu, 08 Oct 2020 20:09:04: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 08 Oct 2020 20:09:07: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 20:09:23: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.10_peaks.xls INFO @ Thu, 08 Oct 2020 20:09:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.10_peaks.narrowPeak INFO @ Thu, 08 Oct 2020 20:09:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.10_summits.bed INFO @ Thu, 08 Oct 2020 20:09:24: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (6222 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:09:35: #3 Call peaks for each chromosome... INFO @ Thu, 08 Oct 2020 20:09:51: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.20_peaks.xls INFO @ Thu, 08 Oct 2020 20:09:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.20_peaks.narrowPeak INFO @ Thu, 08 Oct 2020 20:09:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8832068/SRX8832068.20_summits.bed INFO @ Thu, 08 Oct 2020 20:09:51: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (4347 records, 4 fields): 5 millis CompletedMACS2peakCalling