Job ID = 14160538 SRX = SRX8582551 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:52 49522675 reads; of these: 49522675 (100.00%) were unpaired; of these: 477447 (0.96%) aligned 0 times 40202274 (81.18%) aligned exactly 1 time 8842954 (17.86%) aligned >1 times 99.04% overall alignment rate Time searching: 00:10:52 Overall time: 00:10:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12578916 / 49045228 = 0.2565 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:23:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:23:31: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:23:31: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:23:36: 1000000 INFO @ Thu, 09 Dec 2021 03:23:40: 2000000 INFO @ Thu, 09 Dec 2021 03:23:44: 3000000 INFO @ Thu, 09 Dec 2021 03:23:49: 4000000 INFO @ Thu, 09 Dec 2021 03:23:53: 5000000 INFO @ Thu, 09 Dec 2021 03:23:58: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:24:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:24:01: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:24:01: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:24:02: 7000000 INFO @ Thu, 09 Dec 2021 03:24:06: 8000000 INFO @ Thu, 09 Dec 2021 03:24:06: 1000000 INFO @ Thu, 09 Dec 2021 03:24:11: 9000000 INFO @ Thu, 09 Dec 2021 03:24:12: 2000000 INFO @ Thu, 09 Dec 2021 03:24:15: 10000000 INFO @ Thu, 09 Dec 2021 03:24:17: 3000000 INFO @ Thu, 09 Dec 2021 03:24:20: 11000000 INFO @ Thu, 09 Dec 2021 03:24:22: 4000000 INFO @ Thu, 09 Dec 2021 03:24:24: 12000000 INFO @ Thu, 09 Dec 2021 03:24:27: 5000000 INFO @ Thu, 09 Dec 2021 03:24:29: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:24:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:24:31: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:24:31: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:24:32: 6000000 INFO @ Thu, 09 Dec 2021 03:24:33: 14000000 INFO @ Thu, 09 Dec 2021 03:24:36: 1000000 INFO @ Thu, 09 Dec 2021 03:24:37: 7000000 INFO @ Thu, 09 Dec 2021 03:24:37: 15000000 INFO @ Thu, 09 Dec 2021 03:24:40: 2000000 INFO @ Thu, 09 Dec 2021 03:24:42: 16000000 INFO @ Thu, 09 Dec 2021 03:24:43: 8000000 INFO @ Thu, 09 Dec 2021 03:24:45: 3000000 INFO @ Thu, 09 Dec 2021 03:24:46: 17000000 INFO @ Thu, 09 Dec 2021 03:24:48: 9000000 INFO @ Thu, 09 Dec 2021 03:24:49: 4000000 INFO @ Thu, 09 Dec 2021 03:24:51: 18000000 INFO @ Thu, 09 Dec 2021 03:24:53: 10000000 INFO @ Thu, 09 Dec 2021 03:24:54: 5000000 INFO @ Thu, 09 Dec 2021 03:24:55: 19000000 INFO @ Thu, 09 Dec 2021 03:24:58: 11000000 INFO @ Thu, 09 Dec 2021 03:24:58: 6000000 INFO @ Thu, 09 Dec 2021 03:25:00: 20000000 INFO @ Thu, 09 Dec 2021 03:25:03: 7000000 INFO @ Thu, 09 Dec 2021 03:25:03: 12000000 INFO @ Thu, 09 Dec 2021 03:25:04: 21000000 INFO @ Thu, 09 Dec 2021 03:25:07: 8000000 INFO @ Thu, 09 Dec 2021 03:25:09: 13000000 INFO @ Thu, 09 Dec 2021 03:25:09: 22000000 INFO @ Thu, 09 Dec 2021 03:25:12: 9000000 INFO @ Thu, 09 Dec 2021 03:25:13: 23000000 INFO @ Thu, 09 Dec 2021 03:25:14: 14000000 INFO @ Thu, 09 Dec 2021 03:25:16: 10000000 INFO @ Thu, 09 Dec 2021 03:25:18: 24000000 INFO @ Thu, 09 Dec 2021 03:25:19: 15000000 INFO @ Thu, 09 Dec 2021 03:25:21: 11000000 INFO @ Thu, 09 Dec 2021 03:25:22: 25000000 INFO @ Thu, 09 Dec 2021 03:25:24: 16000000 INFO @ Thu, 09 Dec 2021 03:25:25: 12000000 INFO @ Thu, 09 Dec 2021 03:25:27: 26000000 INFO @ Thu, 09 Dec 2021 03:25:30: 17000000 INFO @ Thu, 09 Dec 2021 03:25:30: 13000000 INFO @ Thu, 09 Dec 2021 03:25:31: 27000000 INFO @ Thu, 09 Dec 2021 03:25:34: 14000000 INFO @ Thu, 09 Dec 2021 03:25:35: 18000000 INFO @ Thu, 09 Dec 2021 03:25:36: 28000000 INFO @ Thu, 09 Dec 2021 03:25:39: 15000000 INFO @ Thu, 09 Dec 2021 03:25:40: 19000000 INFO @ Thu, 09 Dec 2021 03:25:40: 29000000 INFO @ Thu, 09 Dec 2021 03:25:43: 16000000 INFO @ Thu, 09 Dec 2021 03:25:45: 30000000 INFO @ Thu, 09 Dec 2021 03:25:46: 20000000 INFO @ Thu, 09 Dec 2021 03:25:48: 17000000 INFO @ Thu, 09 Dec 2021 03:25:49: 31000000 INFO @ Thu, 09 Dec 2021 03:25:51: 21000000 INFO @ Thu, 09 Dec 2021 03:25:52: 18000000 INFO @ Thu, 09 Dec 2021 03:25:54: 32000000 INFO @ Thu, 09 Dec 2021 03:25:56: 22000000 INFO @ Thu, 09 Dec 2021 03:25:57: 19000000 INFO @ Thu, 09 Dec 2021 03:25:58: 33000000 INFO @ Thu, 09 Dec 2021 03:26:01: 23000000 INFO @ Thu, 09 Dec 2021 03:26:01: 20000000 INFO @ Thu, 09 Dec 2021 03:26:03: 34000000 INFO @ Thu, 09 Dec 2021 03:26:06: 21000000 INFO @ Thu, 09 Dec 2021 03:26:07: 24000000 INFO @ Thu, 09 Dec 2021 03:26:07: 35000000 INFO @ Thu, 09 Dec 2021 03:26:10: 22000000 INFO @ Thu, 09 Dec 2021 03:26:12: 25000000 INFO @ Thu, 09 Dec 2021 03:26:12: 36000000 INFO @ Thu, 09 Dec 2021 03:26:14: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 03:26:14: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 03:26:14: #1 total tags in treatment: 36466312 INFO @ Thu, 09 Dec 2021 03:26:14: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:26:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:26:15: #1 tags after filtering in treatment: 36466312 INFO @ Thu, 09 Dec 2021 03:26:15: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:26:15: #1 finished! INFO @ Thu, 09 Dec 2021 03:26:15: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:26:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:26:15: 23000000 INFO @ Thu, 09 Dec 2021 03:26:17: #2 number of paired peaks: 0 WARNING @ Thu, 09 Dec 2021 03:26:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 03:26:17: Process for pairing-model is terminated! INFO @ Thu, 09 Dec 2021 03:26:17: 26000000 cut: /home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 03:26:19: 24000000 INFO @ Thu, 09 Dec 2021 03:26:22: 27000000 INFO @ Thu, 09 Dec 2021 03:26:24: 25000000 INFO @ Thu, 09 Dec 2021 03:26:28: 28000000 INFO @ Thu, 09 Dec 2021 03:26:28: 26000000 INFO @ Thu, 09 Dec 2021 03:26:33: 27000000 INFO @ Thu, 09 Dec 2021 03:26:33: 29000000 INFO @ Thu, 09 Dec 2021 03:26:37: 28000000 INFO @ Thu, 09 Dec 2021 03:26:38: 30000000 INFO @ Thu, 09 Dec 2021 03:26:42: 29000000 INFO @ Thu, 09 Dec 2021 03:26:43: 31000000 INFO @ Thu, 09 Dec 2021 03:26:46: 30000000 INFO @ Thu, 09 Dec 2021 03:26:49: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 03:26:51: 31000000 INFO @ Thu, 09 Dec 2021 03:26:54: 33000000 INFO @ Thu, 09 Dec 2021 03:26:55: 32000000 INFO @ Thu, 09 Dec 2021 03:26:59: 34000000 INFO @ Thu, 09 Dec 2021 03:27:00: 33000000 INFO @ Thu, 09 Dec 2021 03:27:04: 35000000 INFO @ Thu, 09 Dec 2021 03:27:04: 34000000 INFO @ Thu, 09 Dec 2021 03:27:09: 35000000 INFO @ Thu, 09 Dec 2021 03:27:09: 36000000 INFO @ Thu, 09 Dec 2021 03:27:12: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 03:27:12: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 03:27:12: #1 total tags in treatment: 36466312 INFO @ Thu, 09 Dec 2021 03:27:12: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:27:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:27:13: #1 tags after filtering in treatment: 36466312 INFO @ Thu, 09 Dec 2021 03:27:13: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:27:13: #1 finished! INFO @ Thu, 09 Dec 2021 03:27:13: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:27:13: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:27:13: 36000000 INFO @ Thu, 09 Dec 2021 03:27:15: #2 number of paired peaks: 0 WARNING @ Thu, 09 Dec 2021 03:27:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 03:27:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 03:27:16: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 03:27:16: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 03:27:16: #1 total tags in treatment: 36466312 INFO @ Thu, 09 Dec 2021 03:27:16: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:27:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:27:16: #1 tags after filtering in treatment: 36466312 INFO @ Thu, 09 Dec 2021 03:27:16: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:27:16: #1 finished! INFO @ Thu, 09 Dec 2021 03:27:16: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:27:16: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:27:18: #2 number of paired peaks: 0 WARNING @ Thu, 09 Dec 2021 03:27:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 03:27:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582551/SRX8582551.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。