Job ID = 14160483 SRX = SRX8582549 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:07 47001297 reads; of these: 47001297 (100.00%) were unpaired; of these: 445397 (0.95%) aligned 0 times 38277531 (81.44%) aligned exactly 1 time 8278369 (17.61%) aligned >1 times 99.05% overall alignment rate Time searching: 00:10:08 Overall time: 00:10:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 11478821 / 46555900 = 0.2466 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:05:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:05:18: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:05:18: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:05:24: 1000000 INFO @ Thu, 09 Dec 2021 03:05:30: 2000000 INFO @ Thu, 09 Dec 2021 03:05:37: 3000000 INFO @ Thu, 09 Dec 2021 03:05:43: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:05:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:05:48: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:05:48: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:05:50: 5000000 INFO @ Thu, 09 Dec 2021 03:05:55: 1000000 INFO @ Thu, 09 Dec 2021 03:05:57: 6000000 INFO @ Thu, 09 Dec 2021 03:06:02: 2000000 INFO @ Thu, 09 Dec 2021 03:06:04: 7000000 INFO @ Thu, 09 Dec 2021 03:06:09: 3000000 INFO @ Thu, 09 Dec 2021 03:06:11: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:06:16: 4000000 INFO @ Thu, 09 Dec 2021 03:06:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:06:17: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:06:17: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:06:18: 9000000 INFO @ Thu, 09 Dec 2021 03:06:23: 5000000 INFO @ Thu, 09 Dec 2021 03:06:24: 1000000 INFO @ Thu, 09 Dec 2021 03:06:25: 10000000 INFO @ Thu, 09 Dec 2021 03:06:31: 2000000 INFO @ Thu, 09 Dec 2021 03:06:31: 6000000 INFO @ Thu, 09 Dec 2021 03:06:33: 11000000 INFO @ Thu, 09 Dec 2021 03:06:38: 3000000 INFO @ Thu, 09 Dec 2021 03:06:39: 7000000 INFO @ Thu, 09 Dec 2021 03:06:40: 12000000 INFO @ Thu, 09 Dec 2021 03:06:44: 4000000 INFO @ Thu, 09 Dec 2021 03:06:46: 8000000 INFO @ Thu, 09 Dec 2021 03:06:47: 13000000 INFO @ Thu, 09 Dec 2021 03:06:51: 5000000 INFO @ Thu, 09 Dec 2021 03:06:54: 9000000 INFO @ Thu, 09 Dec 2021 03:06:54: 14000000 INFO @ Thu, 09 Dec 2021 03:06:57: 6000000 INFO @ Thu, 09 Dec 2021 03:07:01: 15000000 INFO @ Thu, 09 Dec 2021 03:07:01: 10000000 INFO @ Thu, 09 Dec 2021 03:07:04: 7000000 INFO @ Thu, 09 Dec 2021 03:07:08: 16000000 INFO @ Thu, 09 Dec 2021 03:07:09: 11000000 INFO @ Thu, 09 Dec 2021 03:07:11: 8000000 INFO @ Thu, 09 Dec 2021 03:07:16: 17000000 INFO @ Thu, 09 Dec 2021 03:07:16: 12000000 INFO @ Thu, 09 Dec 2021 03:07:17: 9000000 INFO @ Thu, 09 Dec 2021 03:07:23: 18000000 INFO @ Thu, 09 Dec 2021 03:07:24: 13000000 INFO @ Thu, 09 Dec 2021 03:07:24: 10000000 INFO @ Thu, 09 Dec 2021 03:07:30: 19000000 INFO @ Thu, 09 Dec 2021 03:07:31: 11000000 INFO @ Thu, 09 Dec 2021 03:07:31: 14000000 INFO @ Thu, 09 Dec 2021 03:07:37: 20000000 INFO @ Thu, 09 Dec 2021 03:07:37: 12000000 INFO @ Thu, 09 Dec 2021 03:07:38: 15000000 INFO @ Thu, 09 Dec 2021 03:07:44: 13000000 INFO @ Thu, 09 Dec 2021 03:07:45: 21000000 INFO @ Thu, 09 Dec 2021 03:07:46: 16000000 INFO @ Thu, 09 Dec 2021 03:07:51: 14000000 INFO @ Thu, 09 Dec 2021 03:07:52: 22000000 INFO @ Thu, 09 Dec 2021 03:07:53: 17000000 INFO @ Thu, 09 Dec 2021 03:07:57: 15000000 INFO @ Thu, 09 Dec 2021 03:07:59: 23000000 INFO @ Thu, 09 Dec 2021 03:08:00: 18000000 INFO @ Thu, 09 Dec 2021 03:08:04: 16000000 INFO @ Thu, 09 Dec 2021 03:08:06: 24000000 INFO @ Thu, 09 Dec 2021 03:08:08: 19000000 INFO @ Thu, 09 Dec 2021 03:08:11: 17000000 INFO @ Thu, 09 Dec 2021 03:08:13: 25000000 INFO @ Thu, 09 Dec 2021 03:08:15: 20000000 INFO @ Thu, 09 Dec 2021 03:08:17: 18000000 INFO @ Thu, 09 Dec 2021 03:08:21: 26000000 INFO @ Thu, 09 Dec 2021 03:08:22: 21000000 INFO @ Thu, 09 Dec 2021 03:08:24: 19000000 INFO @ Thu, 09 Dec 2021 03:08:28: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 03:08:30: 22000000 INFO @ Thu, 09 Dec 2021 03:08:31: 20000000 INFO @ Thu, 09 Dec 2021 03:08:35: 28000000 INFO @ Thu, 09 Dec 2021 03:08:37: 23000000 INFO @ Thu, 09 Dec 2021 03:08:37: 21000000 INFO @ Thu, 09 Dec 2021 03:08:42: 29000000 INFO @ Thu, 09 Dec 2021 03:08:44: 22000000 INFO @ Thu, 09 Dec 2021 03:08:44: 24000000 INFO @ Thu, 09 Dec 2021 03:08:49: 30000000 INFO @ Thu, 09 Dec 2021 03:08:51: 23000000 INFO @ Thu, 09 Dec 2021 03:08:52: 25000000 INFO @ Thu, 09 Dec 2021 03:08:56: 31000000 INFO @ Thu, 09 Dec 2021 03:08:58: 24000000 INFO @ Thu, 09 Dec 2021 03:08:59: 26000000 INFO @ Thu, 09 Dec 2021 03:09:04: 32000000 INFO @ Thu, 09 Dec 2021 03:09:04: 25000000 INFO @ Thu, 09 Dec 2021 03:09:06: 27000000 INFO @ Thu, 09 Dec 2021 03:09:11: 33000000 INFO @ Thu, 09 Dec 2021 03:09:11: 26000000 INFO @ Thu, 09 Dec 2021 03:09:14: 28000000 INFO @ Thu, 09 Dec 2021 03:09:17: 27000000 INFO @ Thu, 09 Dec 2021 03:09:18: 34000000 INFO @ Thu, 09 Dec 2021 03:09:21: 29000000 INFO @ Thu, 09 Dec 2021 03:09:24: 28000000 INFO @ Thu, 09 Dec 2021 03:09:25: 35000000 INFO @ Thu, 09 Dec 2021 03:09:26: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 03:09:26: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 03:09:26: #1 total tags in treatment: 35077079 INFO @ Thu, 09 Dec 2021 03:09:26: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:09:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:09:26: #1 tags after filtering in treatment: 35077079 INFO @ Thu, 09 Dec 2021 03:09:26: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:09:26: #1 finished! INFO @ Thu, 09 Dec 2021 03:09:26: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:09:26: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:09:28: 30000000 INFO @ Thu, 09 Dec 2021 03:09:28: #2 number of paired peaks: 29 WARNING @ Thu, 09 Dec 2021 03:09:28: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 03:09:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 03:09:31: 29000000 BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 03:09:35: 31000000 INFO @ Thu, 09 Dec 2021 03:09:37: 30000000 INFO @ Thu, 09 Dec 2021 03:09:42: 32000000 INFO @ Thu, 09 Dec 2021 03:09:44: 31000000 INFO @ Thu, 09 Dec 2021 03:09:50: 33000000 INFO @ Thu, 09 Dec 2021 03:09:50: 32000000 INFO @ Thu, 09 Dec 2021 03:09:57: 33000000 INFO @ Thu, 09 Dec 2021 03:09:57: 34000000 INFO @ Thu, 09 Dec 2021 03:10:03: 34000000 INFO @ Thu, 09 Dec 2021 03:10:04: 35000000 INFO @ Thu, 09 Dec 2021 03:10:04: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 03:10:04: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 03:10:04: #1 total tags in treatment: 35077079 INFO @ Thu, 09 Dec 2021 03:10:04: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:10:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:10:05: #1 tags after filtering in treatment: 35077079 INFO @ Thu, 09 Dec 2021 03:10:05: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:10:05: #1 finished! INFO @ Thu, 09 Dec 2021 03:10:05: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:10:05: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:10:07: #2 number of paired peaks: 29 WARNING @ Thu, 09 Dec 2021 03:10:07: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 03:10:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 03:10:09: 35000000 INFO @ Thu, 09 Dec 2021 03:10:10: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 03:10:10: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 03:10:10: #1 total tags in treatment: 35077079 INFO @ Thu, 09 Dec 2021 03:10:10: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:10:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:10:10: #1 tags after filtering in treatment: 35077079 INFO @ Thu, 09 Dec 2021 03:10:10: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:10:10: #1 finished! INFO @ Thu, 09 Dec 2021 03:10:10: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:10:10: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:10:12: #2 number of paired peaks: 29 WARNING @ Thu, 09 Dec 2021 03:10:12: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 03:10:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX8582549/SRX8582549.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling