Job ID = 14160655 SRX = SRX8331157 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:16 31107446 reads; of these: 31107446 (100.00%) were unpaired; of these: 4844317 (15.57%) aligned 0 times 20585840 (66.18%) aligned exactly 1 time 5677289 (18.25%) aligned >1 times 84.43% overall alignment rate Time searching: 00:12:16 Overall time: 00:12:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 24116242 / 26263129 = 0.9183 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:51:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:51:46: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:51:46: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:51:52: 1000000 INFO @ Thu, 09 Dec 2021 03:51:58: 2000000 INFO @ Thu, 09 Dec 2021 03:51:59: #1 tag size is determined as 65 bps INFO @ Thu, 09 Dec 2021 03:51:59: #1 tag size = 65 INFO @ Thu, 09 Dec 2021 03:51:59: #1 total tags in treatment: 2146887 INFO @ Thu, 09 Dec 2021 03:51:59: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:51:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:51:59: #1 tags after filtering in treatment: 2146887 INFO @ Thu, 09 Dec 2021 03:51:59: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:51:59: #1 finished! INFO @ Thu, 09 Dec 2021 03:51:59: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:51:59: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:51:59: #2 number of paired peaks: 1775 INFO @ Thu, 09 Dec 2021 03:51:59: start model_add_line... INFO @ Thu, 09 Dec 2021 03:51:59: start X-correlation... INFO @ Thu, 09 Dec 2021 03:51:59: end of X-cor INFO @ Thu, 09 Dec 2021 03:51:59: #2 finished! INFO @ Thu, 09 Dec 2021 03:51:59: #2 predicted fragment length is 68 bps INFO @ Thu, 09 Dec 2021 03:51:59: #2 alternative fragment length(s) may be 68 bps INFO @ Thu, 09 Dec 2021 03:51:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.05_model.r WARNING @ Thu, 09 Dec 2021 03:51:59: #2 Since the d (68) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 03:51:59: #2 You may need to consider one of the other alternative d(s): 68 WARNING @ Thu, 09 Dec 2021 03:51:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 03:51:59: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:51:59: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:52:04: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:52:07: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.05_peaks.xls INFO @ Thu, 09 Dec 2021 03:52:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:52:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.05_summits.bed INFO @ Thu, 09 Dec 2021 03:52:07: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (1740 records, 4 fields): 3 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:52:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:52:16: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:52:16: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:52:23: 1000000 INFO @ Thu, 09 Dec 2021 03:52:29: 2000000 INFO @ Thu, 09 Dec 2021 03:52:30: #1 tag size is determined as 65 bps INFO @ Thu, 09 Dec 2021 03:52:30: #1 tag size = 65 INFO @ Thu, 09 Dec 2021 03:52:30: #1 total tags in treatment: 2146887 INFO @ Thu, 09 Dec 2021 03:52:30: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:52:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:52:30: #1 tags after filtering in treatment: 2146887 INFO @ Thu, 09 Dec 2021 03:52:30: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:52:30: #1 finished! INFO @ Thu, 09 Dec 2021 03:52:30: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:52:30: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:52:31: #2 number of paired peaks: 1775 INFO @ Thu, 09 Dec 2021 03:52:31: start model_add_line... INFO @ Thu, 09 Dec 2021 03:52:31: start X-correlation... INFO @ Thu, 09 Dec 2021 03:52:31: end of X-cor INFO @ Thu, 09 Dec 2021 03:52:31: #2 finished! INFO @ Thu, 09 Dec 2021 03:52:31: #2 predicted fragment length is 68 bps INFO @ Thu, 09 Dec 2021 03:52:31: #2 alternative fragment length(s) may be 68 bps INFO @ Thu, 09 Dec 2021 03:52:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.10_model.r WARNING @ Thu, 09 Dec 2021 03:52:31: #2 Since the d (68) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 03:52:31: #2 You may need to consider one of the other alternative d(s): 68 WARNING @ Thu, 09 Dec 2021 03:52:31: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 03:52:31: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:52:31: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:52:36: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:52:38: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.10_peaks.xls INFO @ Thu, 09 Dec 2021 03:52:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:52:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.10_summits.bed INFO @ Thu, 09 Dec 2021 03:52:38: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (978 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:52:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:52:47: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:52:47: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:52:52: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 03:52:58: 2000000 INFO @ Thu, 09 Dec 2021 03:52:58: #1 tag size is determined as 65 bps INFO @ Thu, 09 Dec 2021 03:52:58: #1 tag size = 65 INFO @ Thu, 09 Dec 2021 03:52:58: #1 total tags in treatment: 2146887 INFO @ Thu, 09 Dec 2021 03:52:58: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:52:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:52:58: #1 tags after filtering in treatment: 2146887 INFO @ Thu, 09 Dec 2021 03:52:58: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:52:58: #1 finished! INFO @ Thu, 09 Dec 2021 03:52:58: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:52:58: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:52:59: #2 number of paired peaks: 1775 INFO @ Thu, 09 Dec 2021 03:52:59: start model_add_line... INFO @ Thu, 09 Dec 2021 03:52:59: start X-correlation... INFO @ Thu, 09 Dec 2021 03:52:59: end of X-cor INFO @ Thu, 09 Dec 2021 03:52:59: #2 finished! INFO @ Thu, 09 Dec 2021 03:52:59: #2 predicted fragment length is 68 bps INFO @ Thu, 09 Dec 2021 03:52:59: #2 alternative fragment length(s) may be 68 bps INFO @ Thu, 09 Dec 2021 03:52:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.20_model.r WARNING @ Thu, 09 Dec 2021 03:52:59: #2 Since the d (68) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 03:52:59: #2 You may need to consider one of the other alternative d(s): 68 WARNING @ Thu, 09 Dec 2021 03:52:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 03:52:59: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:52:59: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 03:53:04: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:53:06: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.20_peaks.xls INFO @ Thu, 09 Dec 2021 03:53:06: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:53:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8331157/SRX8331157.20_summits.bed INFO @ Thu, 09 Dec 2021 03:53:06: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (456 records, 4 fields): 16 millis CompletedMACS2peakCalling