Job ID = 14160692 SRX = SRX8331148 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:46 63128850 reads; of these: 63128850 (100.00%) were unpaired; of these: 798346 (1.26%) aligned 0 times 52255651 (82.78%) aligned exactly 1 time 10074853 (15.96%) aligned >1 times 98.74% overall alignment rate Time searching: 00:26:46 Overall time: 00:26:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 51854134 / 62330504 = 0.8319 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 04:27:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 04:27:20: #1 read tag files... INFO @ Thu, 09 Dec 2021 04:27:20: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 04:27:28: 1000000 INFO @ Thu, 09 Dec 2021 04:27:36: 2000000 INFO @ Thu, 09 Dec 2021 04:27:45: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 04:27:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 04:27:50: #1 read tag files... INFO @ Thu, 09 Dec 2021 04:27:50: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 04:27:53: 4000000 INFO @ Thu, 09 Dec 2021 04:27:58: 1000000 INFO @ Thu, 09 Dec 2021 04:28:01: 5000000 INFO @ Thu, 09 Dec 2021 04:28:07: 2000000 INFO @ Thu, 09 Dec 2021 04:28:09: 6000000 INFO @ Thu, 09 Dec 2021 04:28:14: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 04:28:17: 7000000 INFO @ Thu, 09 Dec 2021 04:28:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 04:28:20: #1 read tag files... INFO @ Thu, 09 Dec 2021 04:28:20: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 04:28:23: 4000000 INFO @ Thu, 09 Dec 2021 04:28:26: 8000000 INFO @ Thu, 09 Dec 2021 04:28:29: 1000000 INFO @ Thu, 09 Dec 2021 04:28:31: 5000000 INFO @ Thu, 09 Dec 2021 04:28:35: 9000000 INFO @ Thu, 09 Dec 2021 04:28:38: 2000000 INFO @ Thu, 09 Dec 2021 04:28:40: 6000000 INFO @ Thu, 09 Dec 2021 04:28:46: 10000000 INFO @ Thu, 09 Dec 2021 04:28:46: 3000000 INFO @ Thu, 09 Dec 2021 04:28:48: 7000000 INFO @ Thu, 09 Dec 2021 04:28:50: #1 tag size is determined as 65 bps INFO @ Thu, 09 Dec 2021 04:28:50: #1 tag size = 65 INFO @ Thu, 09 Dec 2021 04:28:50: #1 total tags in treatment: 10476370 INFO @ Thu, 09 Dec 2021 04:28:50: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 04:28:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 04:28:50: #1 tags after filtering in treatment: 10476370 INFO @ Thu, 09 Dec 2021 04:28:50: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 04:28:50: #1 finished! INFO @ Thu, 09 Dec 2021 04:28:50: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 04:28:50: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 04:28:51: #2 number of paired peaks: 762 WARNING @ Thu, 09 Dec 2021 04:28:51: Fewer paired peaks (762) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 762 pairs to build model! INFO @ Thu, 09 Dec 2021 04:28:51: start model_add_line... INFO @ Thu, 09 Dec 2021 04:28:51: start X-correlation... INFO @ Thu, 09 Dec 2021 04:28:51: end of X-cor INFO @ Thu, 09 Dec 2021 04:28:51: #2 finished! INFO @ Thu, 09 Dec 2021 04:28:51: #2 predicted fragment length is 55 bps INFO @ Thu, 09 Dec 2021 04:28:51: #2 alternative fragment length(s) may be 3,55 bps INFO @ Thu, 09 Dec 2021 04:28:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.05_model.r WARNING @ Thu, 09 Dec 2021 04:28:51: #2 Since the d (55) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 04:28:51: #2 You may need to consider one of the other alternative d(s): 3,55 WARNING @ Thu, 09 Dec 2021 04:28:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 04:28:51: #3 Call peaks... INFO @ Thu, 09 Dec 2021 04:28:51: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 04:28:54: 4000000 INFO @ Thu, 09 Dec 2021 04:28:56: 8000000 INFO @ Thu, 09 Dec 2021 04:29:02: 5000000 INFO @ Thu, 09 Dec 2021 04:29:04: 9000000 INFO @ Thu, 09 Dec 2021 04:29:10: 6000000 INFO @ Thu, 09 Dec 2021 04:29:12: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 04:29:13: 10000000 INFO @ Thu, 09 Dec 2021 04:29:17: #1 tag size is determined as 65 bps INFO @ Thu, 09 Dec 2021 04:29:17: #1 tag size = 65 INFO @ Thu, 09 Dec 2021 04:29:17: #1 total tags in treatment: 10476370 INFO @ Thu, 09 Dec 2021 04:29:17: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 04:29:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 04:29:17: #1 tags after filtering in treatment: 10476370 INFO @ Thu, 09 Dec 2021 04:29:17: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 04:29:17: #1 finished! INFO @ Thu, 09 Dec 2021 04:29:17: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 04:29:17: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 04:29:18: #2 number of paired peaks: 762 WARNING @ Thu, 09 Dec 2021 04:29:18: Fewer paired peaks (762) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 762 pairs to build model! INFO @ Thu, 09 Dec 2021 04:29:18: start model_add_line... INFO @ Thu, 09 Dec 2021 04:29:18: start X-correlation... INFO @ Thu, 09 Dec 2021 04:29:18: 7000000 INFO @ Thu, 09 Dec 2021 04:29:18: end of X-cor INFO @ Thu, 09 Dec 2021 04:29:18: #2 finished! INFO @ Thu, 09 Dec 2021 04:29:18: #2 predicted fragment length is 55 bps INFO @ Thu, 09 Dec 2021 04:29:18: #2 alternative fragment length(s) may be 3,55 bps INFO @ Thu, 09 Dec 2021 04:29:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.10_model.r WARNING @ Thu, 09 Dec 2021 04:29:18: #2 Since the d (55) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 04:29:18: #2 You may need to consider one of the other alternative d(s): 3,55 WARNING @ Thu, 09 Dec 2021 04:29:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 04:29:18: #3 Call peaks... INFO @ Thu, 09 Dec 2021 04:29:18: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 04:29:23: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.05_peaks.xls INFO @ Thu, 09 Dec 2021 04:29:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 04:29:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.05_summits.bed INFO @ Thu, 09 Dec 2021 04:29:23: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2215 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 04:29:26: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 04:29:34: 9000000 INFO @ Thu, 09 Dec 2021 04:29:39: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 04:29:42: 10000000 INFO @ Thu, 09 Dec 2021 04:29:46: #1 tag size is determined as 65 bps INFO @ Thu, 09 Dec 2021 04:29:46: #1 tag size = 65 INFO @ Thu, 09 Dec 2021 04:29:46: #1 total tags in treatment: 10476370 INFO @ Thu, 09 Dec 2021 04:29:46: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 04:29:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 04:29:46: #1 tags after filtering in treatment: 10476370 INFO @ Thu, 09 Dec 2021 04:29:46: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 04:29:46: #1 finished! INFO @ Thu, 09 Dec 2021 04:29:46: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 04:29:46: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 04:29:47: #2 number of paired peaks: 762 WARNING @ Thu, 09 Dec 2021 04:29:47: Fewer paired peaks (762) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 762 pairs to build model! INFO @ Thu, 09 Dec 2021 04:29:47: start model_add_line... INFO @ Thu, 09 Dec 2021 04:29:47: start X-correlation... INFO @ Thu, 09 Dec 2021 04:29:48: end of X-cor INFO @ Thu, 09 Dec 2021 04:29:48: #2 finished! INFO @ Thu, 09 Dec 2021 04:29:48: #2 predicted fragment length is 55 bps INFO @ Thu, 09 Dec 2021 04:29:48: #2 alternative fragment length(s) may be 3,55 bps INFO @ Thu, 09 Dec 2021 04:29:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.20_model.r WARNING @ Thu, 09 Dec 2021 04:29:48: #2 Since the d (55) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 04:29:48: #2 You may need to consider one of the other alternative d(s): 3,55 WARNING @ Thu, 09 Dec 2021 04:29:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 04:29:48: #3 Call peaks... INFO @ Thu, 09 Dec 2021 04:29:48: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 04:29:50: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.10_peaks.xls INFO @ Thu, 09 Dec 2021 04:29:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 04:29:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.10_summits.bed INFO @ Thu, 09 Dec 2021 04:29:50: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (956 records, 4 fields): 3 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 04:30:08: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 04:30:19: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.20_peaks.xls INFO @ Thu, 09 Dec 2021 04:30:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 04:30:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8331148/SRX8331148.20_summits.bed INFO @ Thu, 09 Dec 2021 04:30:19: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (366 records, 4 fields): 2 millis CompletedMACS2peakCalling