Job ID = 14160516 SRX = SRX8151886 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:36 20838966 reads; of these: 20838966 (100.00%) were unpaired; of these: 871456 (4.18%) aligned 0 times 16564293 (79.49%) aligned exactly 1 time 3403217 (16.33%) aligned >1 times 95.82% overall alignment rate Time searching: 00:04:36 Overall time: 00:04:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6359070 / 19967510 = 0.3185 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:03:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:03:51: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:03:51: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:03:58: 1000000 INFO @ Thu, 09 Dec 2021 03:04:05: 2000000 INFO @ Thu, 09 Dec 2021 03:04:12: 3000000 INFO @ Thu, 09 Dec 2021 03:04:18: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:04:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:04:20: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:04:20: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:04:26: 5000000 INFO @ Thu, 09 Dec 2021 03:04:27: 1000000 INFO @ Thu, 09 Dec 2021 03:04:33: 6000000 INFO @ Thu, 09 Dec 2021 03:04:34: 2000000 INFO @ Thu, 09 Dec 2021 03:04:41: 7000000 INFO @ Thu, 09 Dec 2021 03:04:41: 3000000 INFO @ Thu, 09 Dec 2021 03:04:48: 4000000 INFO @ Thu, 09 Dec 2021 03:04:48: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:04:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:04:51: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:04:51: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:04:55: 5000000 INFO @ Thu, 09 Dec 2021 03:04:56: 9000000 INFO @ Thu, 09 Dec 2021 03:04:59: 1000000 INFO @ Thu, 09 Dec 2021 03:05:02: 6000000 INFO @ Thu, 09 Dec 2021 03:05:05: 10000000 INFO @ Thu, 09 Dec 2021 03:05:07: 2000000 INFO @ Thu, 09 Dec 2021 03:05:09: 7000000 INFO @ Thu, 09 Dec 2021 03:05:13: 11000000 INFO @ Thu, 09 Dec 2021 03:05:16: 3000000 INFO @ Thu, 09 Dec 2021 03:05:17: 8000000 INFO @ Thu, 09 Dec 2021 03:05:21: 12000000 INFO @ Thu, 09 Dec 2021 03:05:23: 4000000 INFO @ Thu, 09 Dec 2021 03:05:24: 9000000 INFO @ Thu, 09 Dec 2021 03:05:29: 13000000 INFO @ Thu, 09 Dec 2021 03:05:31: 10000000 INFO @ Thu, 09 Dec 2021 03:05:31: 5000000 INFO @ Thu, 09 Dec 2021 03:05:34: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 03:05:34: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 03:05:34: #1 total tags in treatment: 13608440 INFO @ Thu, 09 Dec 2021 03:05:34: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:05:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:05:34: #1 tags after filtering in treatment: 13608440 INFO @ Thu, 09 Dec 2021 03:05:34: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:05:34: #1 finished! INFO @ Thu, 09 Dec 2021 03:05:34: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:05:34: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:05:35: #2 number of paired peaks: 3371 INFO @ Thu, 09 Dec 2021 03:05:35: start model_add_line... INFO @ Thu, 09 Dec 2021 03:05:35: start X-correlation... INFO @ Thu, 09 Dec 2021 03:05:35: end of X-cor INFO @ Thu, 09 Dec 2021 03:05:35: #2 finished! INFO @ Thu, 09 Dec 2021 03:05:35: #2 predicted fragment length is 268 bps INFO @ Thu, 09 Dec 2021 03:05:35: #2 alternative fragment length(s) may be 268 bps INFO @ Thu, 09 Dec 2021 03:05:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.05_model.r INFO @ Thu, 09 Dec 2021 03:05:35: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:05:35: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:05:38: 11000000 INFO @ Thu, 09 Dec 2021 03:05:39: 6000000 INFO @ Thu, 09 Dec 2021 03:05:45: 12000000 INFO @ Thu, 09 Dec 2021 03:05:47: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 03:05:52: 13000000 INFO @ Thu, 09 Dec 2021 03:05:55: 8000000 INFO @ Thu, 09 Dec 2021 03:05:56: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 03:05:56: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 03:05:56: #1 total tags in treatment: 13608440 INFO @ Thu, 09 Dec 2021 03:05:56: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:05:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:05:57: #1 tags after filtering in treatment: 13608440 INFO @ Thu, 09 Dec 2021 03:05:57: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:05:57: #1 finished! INFO @ Thu, 09 Dec 2021 03:05:57: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:05:57: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:05:58: #2 number of paired peaks: 3371 INFO @ Thu, 09 Dec 2021 03:05:58: start model_add_line... INFO @ Thu, 09 Dec 2021 03:05:58: start X-correlation... INFO @ Thu, 09 Dec 2021 03:05:58: end of X-cor INFO @ Thu, 09 Dec 2021 03:05:58: #2 finished! INFO @ Thu, 09 Dec 2021 03:05:58: #2 predicted fragment length is 268 bps INFO @ Thu, 09 Dec 2021 03:05:58: #2 alternative fragment length(s) may be 268 bps INFO @ Thu, 09 Dec 2021 03:05:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.10_model.r INFO @ Thu, 09 Dec 2021 03:05:58: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:05:58: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:06:02: 9000000 INFO @ Thu, 09 Dec 2021 03:06:09: 10000000 BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 03:06:17: 11000000 INFO @ Thu, 09 Dec 2021 03:06:20: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:06:24: 12000000 INFO @ Thu, 09 Dec 2021 03:06:31: 13000000 INFO @ Thu, 09 Dec 2021 03:06:35: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 03:06:35: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 03:06:35: #1 total tags in treatment: 13608440 INFO @ Thu, 09 Dec 2021 03:06:35: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:06:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:06:35: #1 tags after filtering in treatment: 13608440 INFO @ Thu, 09 Dec 2021 03:06:35: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:06:35: #1 finished! INFO @ Thu, 09 Dec 2021 03:06:35: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:06:35: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:06:36: #2 number of paired peaks: 3371 INFO @ Thu, 09 Dec 2021 03:06:36: start model_add_line... INFO @ Thu, 09 Dec 2021 03:06:36: start X-correlation... INFO @ Thu, 09 Dec 2021 03:06:36: end of X-cor INFO @ Thu, 09 Dec 2021 03:06:36: #2 finished! INFO @ Thu, 09 Dec 2021 03:06:36: #2 predicted fragment length is 268 bps INFO @ Thu, 09 Dec 2021 03:06:36: #2 alternative fragment length(s) may be 268 bps INFO @ Thu, 09 Dec 2021 03:06:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.20_model.r INFO @ Thu, 09 Dec 2021 03:06:36: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:06:36: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:06:36: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.05_peaks.xls INFO @ Thu, 09 Dec 2021 03:06:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:06:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.05_summits.bed INFO @ Thu, 09 Dec 2021 03:06:37: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (10953 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 03:06:42: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:06:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.10_peaks.xls INFO @ Thu, 09 Dec 2021 03:06:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:06:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.10_summits.bed INFO @ Thu, 09 Dec 2021 03:06:58: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (8618 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 03:07:21: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:07:37: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.20_peaks.xls INFO @ Thu, 09 Dec 2021 03:07:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:07:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8151886/SRX8151886.20_summits.bed INFO @ Thu, 09 Dec 2021 03:07:38: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (6145 records, 4 fields): 8 millis CompletedMACS2peakCalling