Job ID = 14160523 SRX = SRX8151885 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:06:53 30744808 reads; of these: 30744808 (100.00%) were unpaired; of these: 5765141 (18.75%) aligned 0 times 21316620 (69.33%) aligned exactly 1 time 3663047 (11.91%) aligned >1 times 81.25% overall alignment rate Time searching: 00:06:54 Overall time: 00:06:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8706266 / 24979667 = 0.3485 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:11:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:11:26: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:11:26: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:11:33: 1000000 INFO @ Thu, 09 Dec 2021 03:11:41: 2000000 INFO @ Thu, 09 Dec 2021 03:11:48: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:11:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:11:55: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:11:55: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:11:56: 4000000 INFO @ Thu, 09 Dec 2021 03:12:03: 1000000 INFO @ Thu, 09 Dec 2021 03:12:04: 5000000 INFO @ Thu, 09 Dec 2021 03:12:11: 2000000 INFO @ Thu, 09 Dec 2021 03:12:12: 6000000 INFO @ Thu, 09 Dec 2021 03:12:20: 3000000 INFO @ Thu, 09 Dec 2021 03:12:20: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:12:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:12:25: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:12:25: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:12:27: 4000000 INFO @ Thu, 09 Dec 2021 03:12:28: 8000000 INFO @ Thu, 09 Dec 2021 03:12:32: 1000000 INFO @ Thu, 09 Dec 2021 03:12:36: 5000000 INFO @ Thu, 09 Dec 2021 03:12:37: 9000000 INFO @ Thu, 09 Dec 2021 03:12:40: 2000000 INFO @ Thu, 09 Dec 2021 03:12:44: 6000000 INFO @ Thu, 09 Dec 2021 03:12:45: 10000000 INFO @ Thu, 09 Dec 2021 03:12:48: 3000000 INFO @ Thu, 09 Dec 2021 03:12:52: 7000000 INFO @ Thu, 09 Dec 2021 03:12:52: 11000000 INFO @ Thu, 09 Dec 2021 03:12:56: 4000000 INFO @ Thu, 09 Dec 2021 03:12:59: 8000000 INFO @ Thu, 09 Dec 2021 03:13:00: 12000000 INFO @ Thu, 09 Dec 2021 03:13:03: 5000000 INFO @ Thu, 09 Dec 2021 03:13:07: 9000000 INFO @ Thu, 09 Dec 2021 03:13:08: 13000000 INFO @ Thu, 09 Dec 2021 03:13:11: 6000000 INFO @ Thu, 09 Dec 2021 03:13:14: 10000000 INFO @ Thu, 09 Dec 2021 03:13:16: 14000000 INFO @ Thu, 09 Dec 2021 03:13:19: 7000000 INFO @ Thu, 09 Dec 2021 03:13:22: 11000000 INFO @ Thu, 09 Dec 2021 03:13:24: 15000000 INFO @ Thu, 09 Dec 2021 03:13:27: 8000000 INFO @ Thu, 09 Dec 2021 03:13:29: 12000000 INFO @ Thu, 09 Dec 2021 03:13:32: 16000000 INFO @ Thu, 09 Dec 2021 03:13:34: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 03:13:34: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 03:13:34: #1 total tags in treatment: 16273401 INFO @ Thu, 09 Dec 2021 03:13:34: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:13:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:13:35: #1 tags after filtering in treatment: 16273401 INFO @ Thu, 09 Dec 2021 03:13:35: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:13:35: #1 finished! INFO @ Thu, 09 Dec 2021 03:13:35: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:13:35: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:13:36: 9000000 INFO @ Thu, 09 Dec 2021 03:13:36: #2 number of paired peaks: 3673 INFO @ Thu, 09 Dec 2021 03:13:37: start model_add_line... INFO @ Thu, 09 Dec 2021 03:13:37: start X-correlation... INFO @ Thu, 09 Dec 2021 03:13:37: end of X-cor INFO @ Thu, 09 Dec 2021 03:13:37: #2 finished! INFO @ Thu, 09 Dec 2021 03:13:37: #2 predicted fragment length is 242 bps INFO @ Thu, 09 Dec 2021 03:13:37: #2 alternative fragment length(s) may be 242 bps INFO @ Thu, 09 Dec 2021 03:13:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.05_model.r INFO @ Thu, 09 Dec 2021 03:13:37: 13000000 INFO @ Thu, 09 Dec 2021 03:13:37: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:13:37: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:13:44: 10000000 INFO @ Thu, 09 Dec 2021 03:13:44: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 03:13:51: 11000000 INFO @ Thu, 09 Dec 2021 03:13:53: 15000000 INFO @ Thu, 09 Dec 2021 03:13:59: 12000000 INFO @ Thu, 09 Dec 2021 03:14:01: 16000000 INFO @ Thu, 09 Dec 2021 03:14:03: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 03:14:03: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 03:14:03: #1 total tags in treatment: 16273401 INFO @ Thu, 09 Dec 2021 03:14:03: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:14:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:14:04: #1 tags after filtering in treatment: 16273401 INFO @ Thu, 09 Dec 2021 03:14:04: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:14:04: #1 finished! INFO @ Thu, 09 Dec 2021 03:14:04: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:14:04: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:14:05: #2 number of paired peaks: 3673 INFO @ Thu, 09 Dec 2021 03:14:05: start model_add_line... INFO @ Thu, 09 Dec 2021 03:14:05: start X-correlation... INFO @ Thu, 09 Dec 2021 03:14:05: end of X-cor INFO @ Thu, 09 Dec 2021 03:14:05: #2 finished! INFO @ Thu, 09 Dec 2021 03:14:05: #2 predicted fragment length is 242 bps INFO @ Thu, 09 Dec 2021 03:14:05: #2 alternative fragment length(s) may be 242 bps INFO @ Thu, 09 Dec 2021 03:14:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.10_model.r INFO @ Thu, 09 Dec 2021 03:14:06: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:14:06: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:14:07: 13000000 INFO @ Thu, 09 Dec 2021 03:14:14: 14000000 INFO @ Thu, 09 Dec 2021 03:14:22: 15000000 BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 03:14:30: 16000000 INFO @ Thu, 09 Dec 2021 03:14:32: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 03:14:32: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 03:14:32: #1 total tags in treatment: 16273401 INFO @ Thu, 09 Dec 2021 03:14:32: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:14:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:14:32: #1 tags after filtering in treatment: 16273401 INFO @ Thu, 09 Dec 2021 03:14:32: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:14:32: #1 finished! INFO @ Thu, 09 Dec 2021 03:14:32: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:14:32: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:14:34: #2 number of paired peaks: 3673 INFO @ Thu, 09 Dec 2021 03:14:34: start model_add_line... INFO @ Thu, 09 Dec 2021 03:14:34: start X-correlation... INFO @ Thu, 09 Dec 2021 03:14:34: end of X-cor INFO @ Thu, 09 Dec 2021 03:14:34: #2 finished! INFO @ Thu, 09 Dec 2021 03:14:34: #2 predicted fragment length is 242 bps INFO @ Thu, 09 Dec 2021 03:14:34: #2 alternative fragment length(s) may be 242 bps INFO @ Thu, 09 Dec 2021 03:14:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.20_model.r INFO @ Thu, 09 Dec 2021 03:14:34: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:14:34: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:14:42: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:15:05: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.05_peaks.xls INFO @ Thu, 09 Dec 2021 03:15:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:15:05: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.05_summits.bed INFO @ Thu, 09 Dec 2021 03:15:05: Done! pass1 - making usageList (7 chroms): 4 millis pass2 - checking and writing primary data (12697 records, 4 fields): 18 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 03:15:10: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:15:32: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.10_peaks.xls INFO @ Thu, 09 Dec 2021 03:15:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:15:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.10_summits.bed INFO @ Thu, 09 Dec 2021 03:15:32: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (10164 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 03:15:39: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:16:02: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.20_peaks.xls INFO @ Thu, 09 Dec 2021 03:16:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:16:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8151885/SRX8151885.20_summits.bed INFO @ Thu, 09 Dec 2021 03:16:03: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (7547 records, 4 fields): 11 millis CompletedMACS2peakCalling