Job ID = 14160525 SRX = SRX8151884 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:46 43927762 reads; of these: 43927762 (100.00%) were unpaired; of these: 9010196 (20.51%) aligned 0 times 29721755 (67.66%) aligned exactly 1 time 5195811 (11.83%) aligned >1 times 79.49% overall alignment rate Time searching: 00:08:46 Overall time: 00:08:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 15438836 / 34917566 = 0.4422 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:13:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:13:28: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:13:28: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:13:33: 1000000 INFO @ Thu, 09 Dec 2021 03:13:37: 2000000 INFO @ Thu, 09 Dec 2021 03:13:42: 3000000 INFO @ Thu, 09 Dec 2021 03:13:46: 4000000 INFO @ Thu, 09 Dec 2021 03:13:51: 5000000 INFO @ Thu, 09 Dec 2021 03:13:55: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:13:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:13:58: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:13:58: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:14:00: 7000000 INFO @ Thu, 09 Dec 2021 03:14:04: 1000000 INFO @ Thu, 09 Dec 2021 03:14:05: 8000000 INFO @ Thu, 09 Dec 2021 03:14:09: 2000000 INFO @ Thu, 09 Dec 2021 03:14:09: 9000000 INFO @ Thu, 09 Dec 2021 03:14:14: 10000000 INFO @ Thu, 09 Dec 2021 03:14:15: 3000000 INFO @ Thu, 09 Dec 2021 03:14:19: 11000000 INFO @ Thu, 09 Dec 2021 03:14:20: 4000000 INFO @ Thu, 09 Dec 2021 03:14:24: 12000000 INFO @ Thu, 09 Dec 2021 03:14:26: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:14:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:14:28: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:14:28: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:14:29: 13000000 INFO @ Thu, 09 Dec 2021 03:14:31: 6000000 INFO @ Thu, 09 Dec 2021 03:14:33: 1000000 INFO @ Thu, 09 Dec 2021 03:14:34: 14000000 INFO @ Thu, 09 Dec 2021 03:14:37: 7000000 INFO @ Thu, 09 Dec 2021 03:14:38: 2000000 INFO @ Thu, 09 Dec 2021 03:14:38: 15000000 INFO @ Thu, 09 Dec 2021 03:14:42: 8000000 INFO @ Thu, 09 Dec 2021 03:14:43: 3000000 INFO @ Thu, 09 Dec 2021 03:14:43: 16000000 INFO @ Thu, 09 Dec 2021 03:14:48: 9000000 INFO @ Thu, 09 Dec 2021 03:14:48: 4000000 INFO @ Thu, 09 Dec 2021 03:14:48: 17000000 INFO @ Thu, 09 Dec 2021 03:14:53: 5000000 INFO @ Thu, 09 Dec 2021 03:14:53: 18000000 INFO @ Thu, 09 Dec 2021 03:14:53: 10000000 INFO @ Thu, 09 Dec 2021 03:14:58: 6000000 INFO @ Thu, 09 Dec 2021 03:14:58: 19000000 INFO @ Thu, 09 Dec 2021 03:14:58: 11000000 INFO @ Thu, 09 Dec 2021 03:15:00: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 03:15:00: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 03:15:00: #1 total tags in treatment: 19478730 INFO @ Thu, 09 Dec 2021 03:15:00: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:15:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:15:00: #1 tags after filtering in treatment: 19478730 INFO @ Thu, 09 Dec 2021 03:15:00: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:15:00: #1 finished! INFO @ Thu, 09 Dec 2021 03:15:00: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:15:00: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:15:02: #2 number of paired peaks: 3040 INFO @ Thu, 09 Dec 2021 03:15:02: start model_add_line... INFO @ Thu, 09 Dec 2021 03:15:02: start X-correlation... INFO @ Thu, 09 Dec 2021 03:15:02: end of X-cor INFO @ Thu, 09 Dec 2021 03:15:02: #2 finished! INFO @ Thu, 09 Dec 2021 03:15:02: #2 predicted fragment length is 250 bps INFO @ Thu, 09 Dec 2021 03:15:02: #2 alternative fragment length(s) may be 4,250 bps INFO @ Thu, 09 Dec 2021 03:15:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.05_model.r INFO @ Thu, 09 Dec 2021 03:15:02: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:15:02: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:15:03: 7000000 INFO @ Thu, 09 Dec 2021 03:15:04: 12000000 INFO @ Thu, 09 Dec 2021 03:15:07: 8000000 INFO @ Thu, 09 Dec 2021 03:15:09: 13000000 INFO @ Thu, 09 Dec 2021 03:15:12: 9000000 INFO @ Thu, 09 Dec 2021 03:15:15: 14000000 INFO @ Thu, 09 Dec 2021 03:15:17: 10000000 INFO @ Thu, 09 Dec 2021 03:15:20: 15000000 INFO @ Thu, 09 Dec 2021 03:15:22: 11000000 INFO @ Thu, 09 Dec 2021 03:15:26: 16000000 INFO @ Thu, 09 Dec 2021 03:15:27: 12000000 INFO @ Thu, 09 Dec 2021 03:15:31: 17000000 INFO @ Thu, 09 Dec 2021 03:15:31: 13000000 INFO @ Thu, 09 Dec 2021 03:15:36: 14000000 INFO @ Thu, 09 Dec 2021 03:15:37: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 03:15:41: 15000000 INFO @ Thu, 09 Dec 2021 03:15:42: 19000000 INFO @ Thu, 09 Dec 2021 03:15:45: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 03:15:45: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 03:15:45: #1 total tags in treatment: 19478730 INFO @ Thu, 09 Dec 2021 03:15:45: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:15:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:15:45: #1 tags after filtering in treatment: 19478730 INFO @ Thu, 09 Dec 2021 03:15:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:15:45: #1 finished! INFO @ Thu, 09 Dec 2021 03:15:45: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:15:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:15:46: 16000000 INFO @ Thu, 09 Dec 2021 03:15:47: #2 number of paired peaks: 3040 INFO @ Thu, 09 Dec 2021 03:15:47: start model_add_line... INFO @ Thu, 09 Dec 2021 03:15:47: start X-correlation... INFO @ Thu, 09 Dec 2021 03:15:47: end of X-cor INFO @ Thu, 09 Dec 2021 03:15:47: #2 finished! INFO @ Thu, 09 Dec 2021 03:15:47: #2 predicted fragment length is 250 bps INFO @ Thu, 09 Dec 2021 03:15:47: #2 alternative fragment length(s) may be 4,250 bps INFO @ Thu, 09 Dec 2021 03:15:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.10_model.r INFO @ Thu, 09 Dec 2021 03:15:47: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:15:47: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:15:51: 17000000 INFO @ Thu, 09 Dec 2021 03:15:56: 18000000 INFO @ Thu, 09 Dec 2021 03:15:58: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:16:00: 19000000 INFO @ Thu, 09 Dec 2021 03:16:03: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 03:16:03: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 03:16:03: #1 total tags in treatment: 19478730 INFO @ Thu, 09 Dec 2021 03:16:03: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:16:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:16:03: #1 tags after filtering in treatment: 19478730 INFO @ Thu, 09 Dec 2021 03:16:03: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:16:03: #1 finished! INFO @ Thu, 09 Dec 2021 03:16:03: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:16:03: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:16:05: #2 number of paired peaks: 3040 INFO @ Thu, 09 Dec 2021 03:16:05: start model_add_line... INFO @ Thu, 09 Dec 2021 03:16:05: start X-correlation... INFO @ Thu, 09 Dec 2021 03:16:05: end of X-cor INFO @ Thu, 09 Dec 2021 03:16:05: #2 finished! INFO @ Thu, 09 Dec 2021 03:16:05: #2 predicted fragment length is 250 bps INFO @ Thu, 09 Dec 2021 03:16:05: #2 alternative fragment length(s) may be 4,250 bps INFO @ Thu, 09 Dec 2021 03:16:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.20_model.r INFO @ Thu, 09 Dec 2021 03:16:05: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:16:05: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 03:16:16: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.05_peaks.xls INFO @ Thu, 09 Dec 2021 03:16:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:16:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.05_summits.bed INFO @ Thu, 09 Dec 2021 03:16:17: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (14496 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 03:16:45: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:17:01: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:17:03: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.10_peaks.xls INFO @ Thu, 09 Dec 2021 03:17:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:17:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.10_summits.bed INFO @ Thu, 09 Dec 2021 03:17:03: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (11949 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 03:17:18: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.20_peaks.xls INFO @ Thu, 09 Dec 2021 03:17:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:17:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8151884/SRX8151884.20_summits.bed INFO @ Thu, 09 Dec 2021 03:17:19: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (9051 records, 4 fields): 10 millis CompletedMACS2peakCalling