Job ID = 14160448 SRX = SRX8151844 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:47 30332501 reads; of these: 30332501 (100.00%) were unpaired; of these: 4378717 (14.44%) aligned 0 times 20765884 (68.46%) aligned exactly 1 time 5187900 (17.10%) aligned >1 times 85.56% overall alignment rate Time searching: 00:06:47 Overall time: 00:06:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9421726 / 25953784 = 0.3630 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:46:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:46:46: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:46:46: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:46:51: 1000000 INFO @ Thu, 09 Dec 2021 02:46:56: 2000000 INFO @ Thu, 09 Dec 2021 02:47:01: 3000000 INFO @ Thu, 09 Dec 2021 02:47:06: 4000000 INFO @ Thu, 09 Dec 2021 02:47:11: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:47:15: 6000000 INFO @ Thu, 09 Dec 2021 02:47:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:47:16: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:47:16: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:47:20: 7000000 INFO @ Thu, 09 Dec 2021 02:47:23: 1000000 INFO @ Thu, 09 Dec 2021 02:47:25: 8000000 INFO @ Thu, 09 Dec 2021 02:47:29: 2000000 INFO @ Thu, 09 Dec 2021 02:47:30: 9000000 INFO @ Thu, 09 Dec 2021 02:47:35: 10000000 INFO @ Thu, 09 Dec 2021 02:47:35: 3000000 INFO @ Thu, 09 Dec 2021 02:47:40: 11000000 INFO @ Thu, 09 Dec 2021 02:47:41: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:47:45: 12000000 INFO @ Thu, 09 Dec 2021 02:47:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:47:46: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:47:46: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:47:48: 5000000 INFO @ Thu, 09 Dec 2021 02:47:51: 13000000 INFO @ Thu, 09 Dec 2021 02:47:52: 1000000 INFO @ Thu, 09 Dec 2021 02:47:54: 6000000 INFO @ Thu, 09 Dec 2021 02:47:57: 14000000 INFO @ Thu, 09 Dec 2021 02:47:57: 2000000 INFO @ Thu, 09 Dec 2021 02:48:01: 7000000 INFO @ Thu, 09 Dec 2021 02:48:03: 15000000 INFO @ Thu, 09 Dec 2021 02:48:03: 3000000 INFO @ Thu, 09 Dec 2021 02:48:07: 8000000 INFO @ Thu, 09 Dec 2021 02:48:08: 16000000 INFO @ Thu, 09 Dec 2021 02:48:09: 4000000 INFO @ Thu, 09 Dec 2021 02:48:11: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 02:48:11: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 02:48:11: #1 total tags in treatment: 16532058 INFO @ Thu, 09 Dec 2021 02:48:11: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:48:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:48:12: #1 tags after filtering in treatment: 16532058 INFO @ Thu, 09 Dec 2021 02:48:12: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:48:12: #1 finished! INFO @ Thu, 09 Dec 2021 02:48:12: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:48:12: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:48:13: #2 number of paired peaks: 3420 INFO @ Thu, 09 Dec 2021 02:48:13: start model_add_line... INFO @ Thu, 09 Dec 2021 02:48:13: start X-correlation... INFO @ Thu, 09 Dec 2021 02:48:13: end of X-cor INFO @ Thu, 09 Dec 2021 02:48:13: #2 finished! INFO @ Thu, 09 Dec 2021 02:48:13: #2 predicted fragment length is 201 bps INFO @ Thu, 09 Dec 2021 02:48:13: #2 alternative fragment length(s) may be 201 bps INFO @ Thu, 09 Dec 2021 02:48:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.05_model.r INFO @ Thu, 09 Dec 2021 02:48:13: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:48:13: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 02:48:13: 9000000 INFO @ Thu, 09 Dec 2021 02:48:14: 5000000 INFO @ Thu, 09 Dec 2021 02:48:19: 6000000 INFO @ Thu, 09 Dec 2021 02:48:20: 10000000 INFO @ Thu, 09 Dec 2021 02:48:24: 7000000 INFO @ Thu, 09 Dec 2021 02:48:26: 11000000 INFO @ Thu, 09 Dec 2021 02:48:29: 8000000 INFO @ Thu, 09 Dec 2021 02:48:33: 12000000 INFO @ Thu, 09 Dec 2021 02:48:34: 9000000 INFO @ Thu, 09 Dec 2021 02:48:39: 10000000 INFO @ Thu, 09 Dec 2021 02:48:39: 13000000 INFO @ Thu, 09 Dec 2021 02:48:44: 11000000 INFO @ Thu, 09 Dec 2021 02:48:45: 14000000 INFO @ Thu, 09 Dec 2021 02:48:49: 12000000 INFO @ Thu, 09 Dec 2021 02:48:51: 15000000 INFO @ Thu, 09 Dec 2021 02:48:54: 13000000 INFO @ Thu, 09 Dec 2021 02:48:58: 16000000 INFO @ Thu, 09 Dec 2021 02:48:58: 14000000 INFO @ Thu, 09 Dec 2021 02:49:01: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 02:49:01: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 02:49:01: #1 total tags in treatment: 16532058 INFO @ Thu, 09 Dec 2021 02:49:01: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:49:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:49:01: #1 tags after filtering in treatment: 16532058 INFO @ Thu, 09 Dec 2021 02:49:01: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:49:01: #1 finished! INFO @ Thu, 09 Dec 2021 02:49:01: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:49:01: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:49:03: #2 number of paired peaks: 3420 INFO @ Thu, 09 Dec 2021 02:49:03: start model_add_line... INFO @ Thu, 09 Dec 2021 02:49:03: start X-correlation... INFO @ Thu, 09 Dec 2021 02:49:03: end of X-cor INFO @ Thu, 09 Dec 2021 02:49:03: #2 finished! INFO @ Thu, 09 Dec 2021 02:49:03: #2 predicted fragment length is 201 bps INFO @ Thu, 09 Dec 2021 02:49:03: #2 alternative fragment length(s) may be 201 bps INFO @ Thu, 09 Dec 2021 02:49:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.10_model.r INFO @ Thu, 09 Dec 2021 02:49:03: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:49:03: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 02:49:03: 15000000 INFO @ Thu, 09 Dec 2021 02:49:08: 16000000 INFO @ Thu, 09 Dec 2021 02:49:09: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 02:49:11: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 02:49:11: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 02:49:11: #1 total tags in treatment: 16532058 INFO @ Thu, 09 Dec 2021 02:49:11: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:49:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:49:11: #1 tags after filtering in treatment: 16532058 INFO @ Thu, 09 Dec 2021 02:49:11: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:49:11: #1 finished! INFO @ Thu, 09 Dec 2021 02:49:11: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:49:11: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:49:13: #2 number of paired peaks: 3420 INFO @ Thu, 09 Dec 2021 02:49:13: start model_add_line... INFO @ Thu, 09 Dec 2021 02:49:13: start X-correlation... INFO @ Thu, 09 Dec 2021 02:49:13: end of X-cor INFO @ Thu, 09 Dec 2021 02:49:13: #2 finished! INFO @ Thu, 09 Dec 2021 02:49:13: #2 predicted fragment length is 201 bps INFO @ Thu, 09 Dec 2021 02:49:13: #2 alternative fragment length(s) may be 201 bps INFO @ Thu, 09 Dec 2021 02:49:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.20_model.r INFO @ Thu, 09 Dec 2021 02:49:13: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:49:13: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 02:49:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.05_peaks.xls INFO @ Thu, 09 Dec 2021 02:49:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:49:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.05_summits.bed INFO @ Thu, 09 Dec 2021 02:49:29: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (12829 records, 4 fields): 17 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 02:50:00: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 02:50:09: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 02:50:20: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.10_peaks.xls INFO @ Thu, 09 Dec 2021 02:50:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:50:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.10_summits.bed INFO @ Thu, 09 Dec 2021 02:50:20: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (9949 records, 4 fields): 25 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:50:28: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.20_peaks.xls INFO @ Thu, 09 Dec 2021 02:50:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:50:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8151844/SRX8151844.20_summits.bed INFO @ Thu, 09 Dec 2021 02:50:28: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (6834 records, 4 fields): 10 millis CompletedMACS2peakCalling