Job ID = 14160450 SRX = SRX8151843 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:08 37091956 reads; of these: 37091956 (100.00%) were unpaired; of these: 3714677 (10.01%) aligned 0 times 27559171 (74.30%) aligned exactly 1 time 5818108 (15.69%) aligned >1 times 89.99% overall alignment rate Time searching: 00:09:08 Overall time: 00:09:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 13283031 / 33377279 = 0.3980 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:52:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:52:08: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:52:08: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:52:15: 1000000 INFO @ Thu, 09 Dec 2021 02:52:22: 2000000 INFO @ Thu, 09 Dec 2021 02:52:28: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:52:35: 4000000 INFO @ Thu, 09 Dec 2021 02:52:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:52:36: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:52:36: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:52:41: 5000000 INFO @ Thu, 09 Dec 2021 02:52:43: 1000000 INFO @ Thu, 09 Dec 2021 02:52:48: 6000000 INFO @ Thu, 09 Dec 2021 02:52:50: 2000000 INFO @ Thu, 09 Dec 2021 02:52:55: 7000000 INFO @ Thu, 09 Dec 2021 02:52:56: 3000000 INFO @ Thu, 09 Dec 2021 02:53:02: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:53:03: 4000000 INFO @ Thu, 09 Dec 2021 02:53:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:53:06: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:53:06: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:53:08: 9000000 INFO @ Thu, 09 Dec 2021 02:53:10: 5000000 INFO @ Thu, 09 Dec 2021 02:53:14: 1000000 INFO @ Thu, 09 Dec 2021 02:53:15: 10000000 INFO @ Thu, 09 Dec 2021 02:53:16: 6000000 INFO @ Thu, 09 Dec 2021 02:53:21: 2000000 INFO @ Thu, 09 Dec 2021 02:53:22: 11000000 INFO @ Thu, 09 Dec 2021 02:53:23: 7000000 INFO @ Thu, 09 Dec 2021 02:53:29: 3000000 INFO @ Thu, 09 Dec 2021 02:53:29: 12000000 INFO @ Thu, 09 Dec 2021 02:53:30: 8000000 INFO @ Thu, 09 Dec 2021 02:53:35: 13000000 INFO @ Thu, 09 Dec 2021 02:53:36: 4000000 INFO @ Thu, 09 Dec 2021 02:53:37: 9000000 INFO @ Thu, 09 Dec 2021 02:53:42: 14000000 INFO @ Thu, 09 Dec 2021 02:53:43: 5000000 INFO @ Thu, 09 Dec 2021 02:53:44: 10000000 INFO @ Thu, 09 Dec 2021 02:53:49: 15000000 INFO @ Thu, 09 Dec 2021 02:53:49: 6000000 INFO @ Thu, 09 Dec 2021 02:53:51: 11000000 INFO @ Thu, 09 Dec 2021 02:53:56: 16000000 INFO @ Thu, 09 Dec 2021 02:53:57: 7000000 INFO @ Thu, 09 Dec 2021 02:53:58: 12000000 INFO @ Thu, 09 Dec 2021 02:54:03: 17000000 INFO @ Thu, 09 Dec 2021 02:54:04: 8000000 INFO @ Thu, 09 Dec 2021 02:54:05: 13000000 INFO @ Thu, 09 Dec 2021 02:54:10: 18000000 INFO @ Thu, 09 Dec 2021 02:54:11: 9000000 INFO @ Thu, 09 Dec 2021 02:54:11: 14000000 INFO @ Thu, 09 Dec 2021 02:54:17: 19000000 INFO @ Thu, 09 Dec 2021 02:54:18: 15000000 INFO @ Thu, 09 Dec 2021 02:54:20: 10000000 INFO @ Thu, 09 Dec 2021 02:54:24: 20000000 INFO @ Thu, 09 Dec 2021 02:54:24: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 02:54:24: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 02:54:24: #1 total tags in treatment: 20094248 INFO @ Thu, 09 Dec 2021 02:54:24: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:54:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:54:25: #1 tags after filtering in treatment: 20094248 INFO @ Thu, 09 Dec 2021 02:54:25: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:54:25: #1 finished! INFO @ Thu, 09 Dec 2021 02:54:25: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:54:25: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:54:25: 16000000 INFO @ Thu, 09 Dec 2021 02:54:27: #2 number of paired peaks: 3001 INFO @ Thu, 09 Dec 2021 02:54:27: start model_add_line... INFO @ Thu, 09 Dec 2021 02:54:27: start X-correlation... INFO @ Thu, 09 Dec 2021 02:54:27: end of X-cor INFO @ Thu, 09 Dec 2021 02:54:27: #2 finished! INFO @ Thu, 09 Dec 2021 02:54:27: #2 predicted fragment length is 256 bps INFO @ Thu, 09 Dec 2021 02:54:27: #2 alternative fragment length(s) may be 4,256 bps INFO @ Thu, 09 Dec 2021 02:54:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.05_model.r INFO @ Thu, 09 Dec 2021 02:54:27: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:54:27: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 02:54:27: 11000000 INFO @ Thu, 09 Dec 2021 02:54:32: 17000000 INFO @ Thu, 09 Dec 2021 02:54:34: 12000000 INFO @ Thu, 09 Dec 2021 02:54:39: 18000000 INFO @ Thu, 09 Dec 2021 02:54:41: 13000000 INFO @ Thu, 09 Dec 2021 02:54:46: 19000000 INFO @ Thu, 09 Dec 2021 02:54:49: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 02:54:53: 20000000 INFO @ Thu, 09 Dec 2021 02:54:54: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 02:54:54: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 02:54:54: #1 total tags in treatment: 20094248 INFO @ Thu, 09 Dec 2021 02:54:54: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:54:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:54:54: #1 tags after filtering in treatment: 20094248 INFO @ Thu, 09 Dec 2021 02:54:54: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:54:54: #1 finished! INFO @ Thu, 09 Dec 2021 02:54:54: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:54:54: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:54:56: 15000000 INFO @ Thu, 09 Dec 2021 02:54:56: #2 number of paired peaks: 3001 INFO @ Thu, 09 Dec 2021 02:54:56: start model_add_line... INFO @ Thu, 09 Dec 2021 02:54:57: start X-correlation... INFO @ Thu, 09 Dec 2021 02:54:57: end of X-cor INFO @ Thu, 09 Dec 2021 02:54:57: #2 finished! INFO @ Thu, 09 Dec 2021 02:54:57: #2 predicted fragment length is 256 bps INFO @ Thu, 09 Dec 2021 02:54:57: #2 alternative fragment length(s) may be 4,256 bps INFO @ Thu, 09 Dec 2021 02:54:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.10_model.r INFO @ Thu, 09 Dec 2021 02:54:57: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:54:57: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 02:55:03: 16000000 INFO @ Thu, 09 Dec 2021 02:55:09: 17000000 INFO @ Thu, 09 Dec 2021 02:55:16: 18000000 INFO @ Thu, 09 Dec 2021 02:55:24: 19000000 INFO @ Thu, 09 Dec 2021 02:55:30: 20000000 INFO @ Thu, 09 Dec 2021 02:55:31: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 02:55:31: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 02:55:31: #1 total tags in treatment: 20094248 INFO @ Thu, 09 Dec 2021 02:55:31: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:55:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:55:32: #1 tags after filtering in treatment: 20094248 INFO @ Thu, 09 Dec 2021 02:55:32: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:55:32: #1 finished! INFO @ Thu, 09 Dec 2021 02:55:32: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:55:32: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:55:33: #2 number of paired peaks: 3001 INFO @ Thu, 09 Dec 2021 02:55:33: start model_add_line... INFO @ Thu, 09 Dec 2021 02:55:34: start X-correlation... INFO @ Thu, 09 Dec 2021 02:55:34: end of X-cor INFO @ Thu, 09 Dec 2021 02:55:34: #2 finished! INFO @ Thu, 09 Dec 2021 02:55:34: #2 predicted fragment length is 256 bps INFO @ Thu, 09 Dec 2021 02:55:34: #2 alternative fragment length(s) may be 4,256 bps INFO @ Thu, 09 Dec 2021 02:55:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.20_model.r INFO @ Thu, 09 Dec 2021 02:55:34: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:55:34: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 02:55:55: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 02:56:19: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.05_peaks.xls INFO @ Thu, 09 Dec 2021 02:56:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:56:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.05_summits.bed INFO @ Thu, 09 Dec 2021 02:56:19: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (13334 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:56:22: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 02:56:45: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.10_peaks.xls INFO @ Thu, 09 Dec 2021 02:56:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:56:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.10_summits.bed INFO @ Thu, 09 Dec 2021 02:56:45: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (10766 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:56:58: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 02:57:19: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.20_peaks.xls INFO @ Thu, 09 Dec 2021 02:57:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:57:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX8151843/SRX8151843.20_summits.bed INFO @ Thu, 09 Dec 2021 02:57:19: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (7851 records, 4 fields): 12 millis CompletedMACS2peakCalling