Job ID = 6368988 SRX = SRX7971813 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:42:46 prefetch.2.10.7: 1) Downloading 'SRR11392699'... 2020-06-16T00:42:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:56:43 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:56:43 prefetch.2.10.7: 1) 'SRR11392699' was downloaded successfully Read 76735594 spots for SRR11392699/SRR11392699.sra Written 76735594 spots for SRR11392699/SRR11392699.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:37 76735594 reads; of these: 76735594 (100.00%) were unpaired; of these: 525087 (0.68%) aligned 0 times 63509151 (82.76%) aligned exactly 1 time 12701356 (16.55%) aligned >1 times 99.32% overall alignment rate Time searching: 00:27:37 Overall time: 00:27:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 18670492 / 76210507 = 0.2450 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:54:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:54:03: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:54:03: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:54:09: 1000000 INFO @ Tue, 16 Jun 2020 10:54:15: 2000000 INFO @ Tue, 16 Jun 2020 10:54:22: 3000000 INFO @ Tue, 16 Jun 2020 10:54:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:54:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:54:33: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:54:33: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:54:35: 5000000 INFO @ Tue, 16 Jun 2020 10:54:40: 1000000 INFO @ Tue, 16 Jun 2020 10:54:42: 6000000 INFO @ Tue, 16 Jun 2020 10:54:47: 2000000 INFO @ Tue, 16 Jun 2020 10:54:48: 7000000 INFO @ Tue, 16 Jun 2020 10:54:55: 3000000 INFO @ Tue, 16 Jun 2020 10:54:55: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:55:02: 4000000 INFO @ Tue, 16 Jun 2020 10:55:02: 9000000 INFO @ Tue, 16 Jun 2020 10:55:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:55:03: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:55:03: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:55:09: 5000000 INFO @ Tue, 16 Jun 2020 10:55:09: 10000000 INFO @ Tue, 16 Jun 2020 10:55:11: 1000000 INFO @ Tue, 16 Jun 2020 10:55:16: 6000000 INFO @ Tue, 16 Jun 2020 10:55:17: 11000000 INFO @ Tue, 16 Jun 2020 10:55:18: 2000000 INFO @ Tue, 16 Jun 2020 10:55:23: 7000000 INFO @ Tue, 16 Jun 2020 10:55:24: 12000000 INFO @ Tue, 16 Jun 2020 10:55:25: 3000000 INFO @ Tue, 16 Jun 2020 10:55:30: 8000000 INFO @ Tue, 16 Jun 2020 10:55:31: 13000000 INFO @ Tue, 16 Jun 2020 10:55:32: 4000000 INFO @ Tue, 16 Jun 2020 10:55:37: 9000000 INFO @ Tue, 16 Jun 2020 10:55:38: 14000000 INFO @ Tue, 16 Jun 2020 10:55:40: 5000000 INFO @ Tue, 16 Jun 2020 10:55:45: 10000000 INFO @ Tue, 16 Jun 2020 10:55:45: 15000000 INFO @ Tue, 16 Jun 2020 10:55:47: 6000000 INFO @ Tue, 16 Jun 2020 10:55:52: 11000000 INFO @ Tue, 16 Jun 2020 10:55:52: 16000000 INFO @ Tue, 16 Jun 2020 10:55:54: 7000000 INFO @ Tue, 16 Jun 2020 10:55:59: 12000000 INFO @ Tue, 16 Jun 2020 10:56:00: 17000000 INFO @ Tue, 16 Jun 2020 10:56:01: 8000000 INFO @ Tue, 16 Jun 2020 10:56:06: 13000000 INFO @ Tue, 16 Jun 2020 10:56:08: 18000000 INFO @ Tue, 16 Jun 2020 10:56:09: 9000000 INFO @ Tue, 16 Jun 2020 10:56:13: 14000000 INFO @ Tue, 16 Jun 2020 10:56:15: 19000000 INFO @ Tue, 16 Jun 2020 10:56:16: 10000000 INFO @ Tue, 16 Jun 2020 10:56:21: 15000000 INFO @ Tue, 16 Jun 2020 10:56:23: 20000000 INFO @ Tue, 16 Jun 2020 10:56:23: 11000000 INFO @ Tue, 16 Jun 2020 10:56:28: 16000000 INFO @ Tue, 16 Jun 2020 10:56:30: 21000000 INFO @ Tue, 16 Jun 2020 10:56:31: 12000000 INFO @ Tue, 16 Jun 2020 10:56:35: 17000000 INFO @ Tue, 16 Jun 2020 10:56:38: 22000000 INFO @ Tue, 16 Jun 2020 10:56:38: 13000000 INFO @ Tue, 16 Jun 2020 10:56:43: 18000000 INFO @ Tue, 16 Jun 2020 10:56:45: 14000000 INFO @ Tue, 16 Jun 2020 10:56:45: 23000000 INFO @ Tue, 16 Jun 2020 10:56:51: 19000000 INFO @ Tue, 16 Jun 2020 10:56:52: 15000000 INFO @ Tue, 16 Jun 2020 10:56:53: 24000000 INFO @ Tue, 16 Jun 2020 10:56:58: 20000000 INFO @ Tue, 16 Jun 2020 10:56:59: 16000000 INFO @ Tue, 16 Jun 2020 10:57:00: 25000000 INFO @ Tue, 16 Jun 2020 10:57:06: 21000000 INFO @ Tue, 16 Jun 2020 10:57:07: 17000000 INFO @ Tue, 16 Jun 2020 10:57:08: 26000000 INFO @ Tue, 16 Jun 2020 10:57:13: 22000000 INFO @ Tue, 16 Jun 2020 10:57:15: 18000000 INFO @ Tue, 16 Jun 2020 10:57:15: 27000000 INFO @ Tue, 16 Jun 2020 10:57:20: 23000000 INFO @ Tue, 16 Jun 2020 10:57:22: 19000000 INFO @ Tue, 16 Jun 2020 10:57:23: 28000000 INFO @ Tue, 16 Jun 2020 10:57:28: 24000000 INFO @ Tue, 16 Jun 2020 10:57:30: 20000000 INFO @ Tue, 16 Jun 2020 10:57:30: 29000000 INFO @ Tue, 16 Jun 2020 10:57:36: 25000000 INFO @ Tue, 16 Jun 2020 10:57:37: 21000000 INFO @ Tue, 16 Jun 2020 10:57:38: 30000000 INFO @ Tue, 16 Jun 2020 10:57:43: 26000000 INFO @ Tue, 16 Jun 2020 10:57:45: 22000000 INFO @ Tue, 16 Jun 2020 10:57:45: 31000000 INFO @ Tue, 16 Jun 2020 10:57:51: 27000000 INFO @ Tue, 16 Jun 2020 10:57:53: 23000000 INFO @ Tue, 16 Jun 2020 10:57:53: 32000000 INFO @ Tue, 16 Jun 2020 10:57:58: 28000000 INFO @ Tue, 16 Jun 2020 10:58:00: 24000000 INFO @ Tue, 16 Jun 2020 10:58:00: 33000000 INFO @ Tue, 16 Jun 2020 10:58:06: 29000000 INFO @ Tue, 16 Jun 2020 10:58:08: 34000000 INFO @ Tue, 16 Jun 2020 10:58:08: 25000000 INFO @ Tue, 16 Jun 2020 10:58:13: 30000000 INFO @ Tue, 16 Jun 2020 10:58:15: 35000000 INFO @ Tue, 16 Jun 2020 10:58:16: 26000000 INFO @ Tue, 16 Jun 2020 10:58:21: 31000000 INFO @ Tue, 16 Jun 2020 10:58:22: 36000000 INFO @ Tue, 16 Jun 2020 10:58:23: 27000000 INFO @ Tue, 16 Jun 2020 10:58:28: 32000000 INFO @ Tue, 16 Jun 2020 10:58:30: 37000000 INFO @ Tue, 16 Jun 2020 10:58:31: 28000000 INFO @ Tue, 16 Jun 2020 10:58:36: 33000000 INFO @ Tue, 16 Jun 2020 10:58:37: 38000000 INFO @ Tue, 16 Jun 2020 10:58:38: 29000000 INFO @ Tue, 16 Jun 2020 10:58:43: 34000000 INFO @ Tue, 16 Jun 2020 10:58:44: 39000000 INFO @ Tue, 16 Jun 2020 10:58:46: 30000000 INFO @ Tue, 16 Jun 2020 10:58:51: 35000000 INFO @ Tue, 16 Jun 2020 10:58:52: 40000000 INFO @ Tue, 16 Jun 2020 10:58:54: 31000000 INFO @ Tue, 16 Jun 2020 10:58:58: 36000000 INFO @ Tue, 16 Jun 2020 10:58:59: 41000000 INFO @ Tue, 16 Jun 2020 10:59:01: 32000000 INFO @ Tue, 16 Jun 2020 10:59:05: 37000000 INFO @ Tue, 16 Jun 2020 10:59:06: 42000000 INFO @ Tue, 16 Jun 2020 10:59:09: 33000000 INFO @ Tue, 16 Jun 2020 10:59:13: 38000000 INFO @ Tue, 16 Jun 2020 10:59:14: 43000000 INFO @ Tue, 16 Jun 2020 10:59:17: 34000000 INFO @ Tue, 16 Jun 2020 10:59:21: 39000000 INFO @ Tue, 16 Jun 2020 10:59:21: 44000000 INFO @ Tue, 16 Jun 2020 10:59:24: 35000000 INFO @ Tue, 16 Jun 2020 10:59:28: 40000000 INFO @ Tue, 16 Jun 2020 10:59:29: 45000000 INFO @ Tue, 16 Jun 2020 10:59:32: 36000000 INFO @ Tue, 16 Jun 2020 10:59:36: 46000000 INFO @ Tue, 16 Jun 2020 10:59:36: 41000000 INFO @ Tue, 16 Jun 2020 10:59:40: 37000000 INFO @ Tue, 16 Jun 2020 10:59:44: 47000000 INFO @ Tue, 16 Jun 2020 10:59:44: 42000000 INFO @ Tue, 16 Jun 2020 10:59:47: 38000000 INFO @ Tue, 16 Jun 2020 10:59:51: 48000000 INFO @ Tue, 16 Jun 2020 10:59:51: 43000000 INFO @ Tue, 16 Jun 2020 10:59:55: 39000000 INFO @ Tue, 16 Jun 2020 10:59:58: 49000000 INFO @ Tue, 16 Jun 2020 10:59:59: 44000000 INFO @ Tue, 16 Jun 2020 11:00:02: 40000000 INFO @ Tue, 16 Jun 2020 11:00:06: 50000000 INFO @ Tue, 16 Jun 2020 11:00:08: 45000000 INFO @ Tue, 16 Jun 2020 11:00:10: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 11:00:13: 51000000 INFO @ Tue, 16 Jun 2020 11:00:16: 46000000 INFO @ Tue, 16 Jun 2020 11:00:17: 42000000 INFO @ Tue, 16 Jun 2020 11:00:20: 52000000 INFO @ Tue, 16 Jun 2020 11:00:24: 47000000 INFO @ Tue, 16 Jun 2020 11:00:24: 43000000 INFO @ Tue, 16 Jun 2020 11:00:28: 53000000 INFO @ Tue, 16 Jun 2020 11:00:32: 44000000 INFO @ Tue, 16 Jun 2020 11:00:32: 48000000 INFO @ Tue, 16 Jun 2020 11:00:35: 54000000 INFO @ Tue, 16 Jun 2020 11:00:39: 45000000 INFO @ Tue, 16 Jun 2020 11:00:40: 49000000 INFO @ Tue, 16 Jun 2020 11:00:42: 55000000 INFO @ Tue, 16 Jun 2020 11:00:46: 46000000 INFO @ Tue, 16 Jun 2020 11:00:48: 50000000 INFO @ Tue, 16 Jun 2020 11:00:49: 56000000 INFO @ Tue, 16 Jun 2020 11:00:54: 47000000 INFO @ Tue, 16 Jun 2020 11:00:56: 51000000 INFO @ Tue, 16 Jun 2020 11:00:57: 57000000 INFO @ Tue, 16 Jun 2020 11:01:01: 48000000 INFO @ Tue, 16 Jun 2020 11:01:01: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 11:01:01: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 11:01:01: #1 total tags in treatment: 57540015 INFO @ Tue, 16 Jun 2020 11:01:01: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:01:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:01:02: #1 tags after filtering in treatment: 57540015 INFO @ Tue, 16 Jun 2020 11:01:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 11:01:02: #1 finished! INFO @ Tue, 16 Jun 2020 11:01:02: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:01:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:01:04: 52000000 INFO @ Tue, 16 Jun 2020 11:01:06: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 11:01:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 11:01:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 11:01:08: 49000000 INFO @ Tue, 16 Jun 2020 11:01:13: 53000000 INFO @ Tue, 16 Jun 2020 11:01:15: 50000000 INFO @ Tue, 16 Jun 2020 11:01:22: 51000000 INFO @ Tue, 16 Jun 2020 11:01:22: 54000000 INFO @ Tue, 16 Jun 2020 11:01:28: 52000000 INFO @ Tue, 16 Jun 2020 11:01:30: 55000000 INFO @ Tue, 16 Jun 2020 11:01:35: 53000000 INFO @ Tue, 16 Jun 2020 11:01:39: 56000000 INFO @ Tue, 16 Jun 2020 11:01:42: 54000000 INFO @ Tue, 16 Jun 2020 11:01:48: 57000000 INFO @ Tue, 16 Jun 2020 11:01:49: 55000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 11:01:54: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 11:01:54: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 11:01:54: #1 total tags in treatment: 57540015 INFO @ Tue, 16 Jun 2020 11:01:54: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:01:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:01:55: #1 tags after filtering in treatment: 57540015 INFO @ Tue, 16 Jun 2020 11:01:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 11:01:55: #1 finished! INFO @ Tue, 16 Jun 2020 11:01:55: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:01:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:01:56: 56000000 INFO @ Tue, 16 Jun 2020 11:01:58: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 11:01:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 11:01:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 11:02:02: 57000000 INFO @ Tue, 16 Jun 2020 11:02:06: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 11:02:06: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 11:02:06: #1 total tags in treatment: 57540015 INFO @ Tue, 16 Jun 2020 11:02:06: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:02:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:02:08: #1 tags after filtering in treatment: 57540015 INFO @ Tue, 16 Jun 2020 11:02:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 11:02:08: #1 finished! INFO @ Tue, 16 Jun 2020 11:02:08: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:02:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:02:11: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 11:02:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 11:02:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971813/SRX7971813.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling