Job ID = 6368985 SRX = SRX7971810 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:34:28 prefetch.2.10.7: 1) Downloading 'SRR11392696'... 2020-06-16T00:34:28 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:43:34 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:43:34 prefetch.2.10.7: 1) 'SRR11392696' was downloaded successfully Read 51944210 spots for SRR11392696/SRR11392696.sra Written 51944210 spots for SRR11392696/SRR11392696.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:14 51944210 reads; of these: 51944210 (100.00%) were unpaired; of these: 1389931 (2.68%) aligned 0 times 45432365 (87.46%) aligned exactly 1 time 5121914 (9.86%) aligned >1 times 97.32% overall alignment rate Time searching: 00:16:14 Overall time: 00:16:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 14926066 / 50554279 = 0.2952 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:15:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:15:02: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:15:02: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:15:09: 1000000 INFO @ Tue, 16 Jun 2020 10:15:15: 2000000 INFO @ Tue, 16 Jun 2020 10:15:22: 3000000 INFO @ Tue, 16 Jun 2020 10:15:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:15:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:15:32: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:15:32: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:15:35: 5000000 INFO @ Tue, 16 Jun 2020 10:15:39: 1000000 INFO @ Tue, 16 Jun 2020 10:15:41: 6000000 INFO @ Tue, 16 Jun 2020 10:15:46: 2000000 INFO @ Tue, 16 Jun 2020 10:15:48: 7000000 INFO @ Tue, 16 Jun 2020 10:15:52: 3000000 INFO @ Tue, 16 Jun 2020 10:15:55: 8000000 INFO @ Tue, 16 Jun 2020 10:15:59: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:16:01: 9000000 INFO @ Tue, 16 Jun 2020 10:16:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:16:02: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:16:02: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:16:05: 5000000 INFO @ Tue, 16 Jun 2020 10:16:08: 10000000 INFO @ Tue, 16 Jun 2020 10:16:09: 1000000 INFO @ Tue, 16 Jun 2020 10:16:12: 6000000 INFO @ Tue, 16 Jun 2020 10:16:14: 11000000 INFO @ Tue, 16 Jun 2020 10:16:16: 2000000 INFO @ Tue, 16 Jun 2020 10:16:19: 7000000 INFO @ Tue, 16 Jun 2020 10:16:21: 12000000 INFO @ Tue, 16 Jun 2020 10:16:22: 3000000 INFO @ Tue, 16 Jun 2020 10:16:26: 8000000 INFO @ Tue, 16 Jun 2020 10:16:28: 13000000 INFO @ Tue, 16 Jun 2020 10:16:29: 4000000 INFO @ Tue, 16 Jun 2020 10:16:32: 9000000 INFO @ Tue, 16 Jun 2020 10:16:34: 14000000 INFO @ Tue, 16 Jun 2020 10:16:35: 5000000 INFO @ Tue, 16 Jun 2020 10:16:39: 10000000 INFO @ Tue, 16 Jun 2020 10:16:41: 15000000 INFO @ Tue, 16 Jun 2020 10:16:42: 6000000 INFO @ Tue, 16 Jun 2020 10:16:46: 11000000 INFO @ Tue, 16 Jun 2020 10:16:48: 16000000 INFO @ Tue, 16 Jun 2020 10:16:49: 7000000 INFO @ Tue, 16 Jun 2020 10:16:52: 12000000 INFO @ Tue, 16 Jun 2020 10:16:54: 17000000 INFO @ Tue, 16 Jun 2020 10:16:55: 8000000 INFO @ Tue, 16 Jun 2020 10:16:59: 13000000 INFO @ Tue, 16 Jun 2020 10:17:01: 18000000 INFO @ Tue, 16 Jun 2020 10:17:02: 9000000 INFO @ Tue, 16 Jun 2020 10:17:06: 14000000 INFO @ Tue, 16 Jun 2020 10:17:08: 19000000 INFO @ Tue, 16 Jun 2020 10:17:08: 10000000 INFO @ Tue, 16 Jun 2020 10:17:13: 15000000 INFO @ Tue, 16 Jun 2020 10:17:14: 20000000 INFO @ Tue, 16 Jun 2020 10:17:15: 11000000 INFO @ Tue, 16 Jun 2020 10:17:19: 16000000 INFO @ Tue, 16 Jun 2020 10:17:21: 21000000 INFO @ Tue, 16 Jun 2020 10:17:21: 12000000 INFO @ Tue, 16 Jun 2020 10:17:26: 17000000 INFO @ Tue, 16 Jun 2020 10:17:28: 22000000 INFO @ Tue, 16 Jun 2020 10:17:28: 13000000 INFO @ Tue, 16 Jun 2020 10:17:32: 18000000 INFO @ Tue, 16 Jun 2020 10:17:34: 23000000 INFO @ Tue, 16 Jun 2020 10:17:35: 14000000 INFO @ Tue, 16 Jun 2020 10:17:39: 19000000 INFO @ Tue, 16 Jun 2020 10:17:41: 24000000 INFO @ Tue, 16 Jun 2020 10:17:41: 15000000 INFO @ Tue, 16 Jun 2020 10:17:46: 20000000 INFO @ Tue, 16 Jun 2020 10:17:48: 16000000 INFO @ Tue, 16 Jun 2020 10:17:48: 25000000 INFO @ Tue, 16 Jun 2020 10:17:52: 21000000 INFO @ Tue, 16 Jun 2020 10:17:54: 17000000 INFO @ Tue, 16 Jun 2020 10:17:54: 26000000 INFO @ Tue, 16 Jun 2020 10:17:59: 22000000 INFO @ Tue, 16 Jun 2020 10:18:01: 18000000 INFO @ Tue, 16 Jun 2020 10:18:01: 27000000 INFO @ Tue, 16 Jun 2020 10:18:06: 23000000 INFO @ Tue, 16 Jun 2020 10:18:07: 19000000 INFO @ Tue, 16 Jun 2020 10:18:08: 28000000 INFO @ Tue, 16 Jun 2020 10:18:13: 24000000 INFO @ Tue, 16 Jun 2020 10:18:14: 20000000 INFO @ Tue, 16 Jun 2020 10:18:15: 29000000 INFO @ Tue, 16 Jun 2020 10:18:19: 25000000 INFO @ Tue, 16 Jun 2020 10:18:21: 21000000 INFO @ Tue, 16 Jun 2020 10:18:21: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:18:26: 26000000 INFO @ Tue, 16 Jun 2020 10:18:27: 22000000 INFO @ Tue, 16 Jun 2020 10:18:28: 31000000 INFO @ Tue, 16 Jun 2020 10:18:33: 27000000 INFO @ Tue, 16 Jun 2020 10:18:34: 23000000 INFO @ Tue, 16 Jun 2020 10:18:35: 32000000 INFO @ Tue, 16 Jun 2020 10:18:40: 28000000 INFO @ Tue, 16 Jun 2020 10:18:40: 24000000 INFO @ Tue, 16 Jun 2020 10:18:41: 33000000 INFO @ Tue, 16 Jun 2020 10:18:46: 29000000 INFO @ Tue, 16 Jun 2020 10:18:47: 25000000 INFO @ Tue, 16 Jun 2020 10:18:48: 34000000 INFO @ Tue, 16 Jun 2020 10:18:53: 30000000 INFO @ Tue, 16 Jun 2020 10:18:54: 26000000 INFO @ Tue, 16 Jun 2020 10:18:55: 35000000 INFO @ Tue, 16 Jun 2020 10:18:59: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:18:59: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:18:59: #1 total tags in treatment: 35628213 INFO @ Tue, 16 Jun 2020 10:18:59: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:18:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:19:00: 31000000 INFO @ Tue, 16 Jun 2020 10:19:00: #1 tags after filtering in treatment: 35628213 INFO @ Tue, 16 Jun 2020 10:19:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:19:00: #1 finished! INFO @ Tue, 16 Jun 2020 10:19:00: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:19:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:19:00: 27000000 INFO @ Tue, 16 Jun 2020 10:19:02: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:19:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:19:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:19:06: 32000000 INFO @ Tue, 16 Jun 2020 10:19:07: 28000000 INFO @ Tue, 16 Jun 2020 10:19:13: 33000000 INFO @ Tue, 16 Jun 2020 10:19:13: 29000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:19:19: 34000000 INFO @ Tue, 16 Jun 2020 10:19:20: 30000000 INFO @ Tue, 16 Jun 2020 10:19:26: 35000000 INFO @ Tue, 16 Jun 2020 10:19:26: 31000000 INFO @ Tue, 16 Jun 2020 10:19:31: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:19:31: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:19:31: #1 total tags in treatment: 35628213 INFO @ Tue, 16 Jun 2020 10:19:31: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:19:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:19:31: #1 tags after filtering in treatment: 35628213 INFO @ Tue, 16 Jun 2020 10:19:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:19:31: #1 finished! INFO @ Tue, 16 Jun 2020 10:19:31: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:19:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:19:33: 32000000 INFO @ Tue, 16 Jun 2020 10:19:33: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:19:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:19:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:19:39: 33000000 INFO @ Tue, 16 Jun 2020 10:19:45: 34000000 INFO @ Tue, 16 Jun 2020 10:19:51: 35000000 INFO @ Tue, 16 Jun 2020 10:19:56: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:19:56: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:19:56: #1 total tags in treatment: 35628213 INFO @ Tue, 16 Jun 2020 10:19:56: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:19:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:19:56: #1 tags after filtering in treatment: 35628213 INFO @ Tue, 16 Jun 2020 10:19:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:19:56: #1 finished! INFO @ Tue, 16 Jun 2020 10:19:56: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:19:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:19:58: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:19:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:19:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971810/SRX7971810.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling