Job ID = 6368984 SRX = SRX7971809 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:36:49 prefetch.2.10.7: 1) Downloading 'SRR11392695'... 2020-06-16T00:36:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:41:35 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:41:35 prefetch.2.10.7: 1) 'SRR11392695' was downloaded successfully Read 27314673 spots for SRR11392695/SRR11392695.sra Written 27314673 spots for SRR11392695/SRR11392695.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:18 27314673 reads; of these: 27314673 (100.00%) were unpaired; of these: 643309 (2.36%) aligned 0 times 24040119 (88.01%) aligned exactly 1 time 2631245 (9.63%) aligned >1 times 97.64% overall alignment rate Time searching: 00:08:18 Overall time: 00:08:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5709783 / 26671364 = 0.2141 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:58:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:58:26: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:58:26: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:58:32: 1000000 INFO @ Tue, 16 Jun 2020 09:58:38: 2000000 INFO @ Tue, 16 Jun 2020 09:58:44: 3000000 INFO @ Tue, 16 Jun 2020 09:58:49: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:58:55: 5000000 INFO @ Tue, 16 Jun 2020 09:58:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:58:56: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:58:56: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:59:01: 6000000 INFO @ Tue, 16 Jun 2020 09:59:02: 1000000 INFO @ Tue, 16 Jun 2020 09:59:07: 7000000 INFO @ Tue, 16 Jun 2020 09:59:08: 2000000 INFO @ Tue, 16 Jun 2020 09:59:13: 8000000 INFO @ Tue, 16 Jun 2020 09:59:14: 3000000 INFO @ Tue, 16 Jun 2020 09:59:19: 9000000 INFO @ Tue, 16 Jun 2020 09:59:20: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:59:25: 10000000 INFO @ Tue, 16 Jun 2020 09:59:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:59:26: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:59:26: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:59:26: 5000000 INFO @ Tue, 16 Jun 2020 09:59:31: 11000000 INFO @ Tue, 16 Jun 2020 09:59:32: 1000000 INFO @ Tue, 16 Jun 2020 09:59:32: 6000000 INFO @ Tue, 16 Jun 2020 09:59:37: 12000000 INFO @ Tue, 16 Jun 2020 09:59:38: 7000000 INFO @ Tue, 16 Jun 2020 09:59:38: 2000000 INFO @ Tue, 16 Jun 2020 09:59:43: 13000000 INFO @ Tue, 16 Jun 2020 09:59:44: 8000000 INFO @ Tue, 16 Jun 2020 09:59:44: 3000000 INFO @ Tue, 16 Jun 2020 09:59:49: 14000000 INFO @ Tue, 16 Jun 2020 09:59:50: 9000000 INFO @ Tue, 16 Jun 2020 09:59:51: 4000000 INFO @ Tue, 16 Jun 2020 09:59:56: 15000000 INFO @ Tue, 16 Jun 2020 09:59:56: 10000000 INFO @ Tue, 16 Jun 2020 09:59:57: 5000000 INFO @ Tue, 16 Jun 2020 10:00:02: 16000000 INFO @ Tue, 16 Jun 2020 10:00:03: 11000000 INFO @ Tue, 16 Jun 2020 10:00:03: 6000000 INFO @ Tue, 16 Jun 2020 10:00:08: 17000000 INFO @ Tue, 16 Jun 2020 10:00:09: 7000000 INFO @ Tue, 16 Jun 2020 10:00:10: 12000000 INFO @ Tue, 16 Jun 2020 10:00:15: 18000000 INFO @ Tue, 16 Jun 2020 10:00:15: 8000000 INFO @ Tue, 16 Jun 2020 10:00:17: 13000000 INFO @ Tue, 16 Jun 2020 10:00:21: 19000000 INFO @ Tue, 16 Jun 2020 10:00:21: 9000000 INFO @ Tue, 16 Jun 2020 10:00:23: 14000000 INFO @ Tue, 16 Jun 2020 10:00:27: 20000000 INFO @ Tue, 16 Jun 2020 10:00:27: 10000000 INFO @ Tue, 16 Jun 2020 10:00:30: 15000000 INFO @ Tue, 16 Jun 2020 10:00:33: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:00:33: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:00:33: #1 total tags in treatment: 20961581 INFO @ Tue, 16 Jun 2020 10:00:33: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:00:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:00:34: 11000000 INFO @ Tue, 16 Jun 2020 10:00:34: #1 tags after filtering in treatment: 20961581 INFO @ Tue, 16 Jun 2020 10:00:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:00:34: #1 finished! INFO @ Tue, 16 Jun 2020 10:00:34: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:00:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:00:35: #2 number of paired peaks: 55 WARNING @ Tue, 16 Jun 2020 10:00:35: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:00:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:00:36: 16000000 INFO @ Tue, 16 Jun 2020 10:00:40: 12000000 INFO @ Tue, 16 Jun 2020 10:00:42: 17000000 INFO @ Tue, 16 Jun 2020 10:00:46: 13000000 INFO @ Tue, 16 Jun 2020 10:00:48: 18000000 INFO @ Tue, 16 Jun 2020 10:00:52: 14000000 INFO @ Tue, 16 Jun 2020 10:00:54: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:00:58: 15000000 INFO @ Tue, 16 Jun 2020 10:01:00: 20000000 INFO @ Tue, 16 Jun 2020 10:01:04: 16000000 INFO @ Tue, 16 Jun 2020 10:01:06: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:01:06: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:01:06: #1 total tags in treatment: 20961581 INFO @ Tue, 16 Jun 2020 10:01:06: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:01:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:01:07: #1 tags after filtering in treatment: 20961581 INFO @ Tue, 16 Jun 2020 10:01:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:01:07: #1 finished! INFO @ Tue, 16 Jun 2020 10:01:07: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:01:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:01:08: #2 number of paired peaks: 55 WARNING @ Tue, 16 Jun 2020 10:01:08: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:01:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:01:10: 17000000 INFO @ Tue, 16 Jun 2020 10:01:16: 18000000 INFO @ Tue, 16 Jun 2020 10:01:21: 19000000 INFO @ Tue, 16 Jun 2020 10:01:27: 20000000 INFO @ Tue, 16 Jun 2020 10:01:33: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:01:33: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:01:33: #1 total tags in treatment: 20961581 INFO @ Tue, 16 Jun 2020 10:01:33: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:01:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:01:33: #1 tags after filtering in treatment: 20961581 INFO @ Tue, 16 Jun 2020 10:01:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:01:33: #1 finished! INFO @ Tue, 16 Jun 2020 10:01:33: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:01:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:01:34: #2 number of paired peaks: 55 WARNING @ Tue, 16 Jun 2020 10:01:34: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:01:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971809/SRX7971809.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。