Job ID = 6368983 SRX = SRX7971808 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:40:03 prefetch.2.10.7: 1) Downloading 'SRR11392694'... 2020-06-16T00:40:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:49:43 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:49:43 prefetch.2.10.7: 1) 'SRR11392694' was downloaded successfully Read 49428249 spots for SRR11392694/SRR11392694.sra Written 49428249 spots for SRR11392694/SRR11392694.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:09 49428249 reads; of these: 49428249 (100.00%) were unpaired; of these: 321676 (0.65%) aligned 0 times 40772516 (82.49%) aligned exactly 1 time 8334057 (16.86%) aligned >1 times 99.35% overall alignment rate Time searching: 00:16:09 Overall time: 00:16:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 9745132 / 49106573 = 0.1984 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:20:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:20:40: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:20:40: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:20:46: 1000000 INFO @ Tue, 16 Jun 2020 10:20:52: 2000000 INFO @ Tue, 16 Jun 2020 10:20:58: 3000000 INFO @ Tue, 16 Jun 2020 10:21:03: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:21:09: 5000000 INFO @ Tue, 16 Jun 2020 10:21:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:21:10: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:21:10: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:21:15: 6000000 INFO @ Tue, 16 Jun 2020 10:21:17: 1000000 INFO @ Tue, 16 Jun 2020 10:21:22: 7000000 INFO @ Tue, 16 Jun 2020 10:21:24: 2000000 INFO @ Tue, 16 Jun 2020 10:21:28: 8000000 INFO @ Tue, 16 Jun 2020 10:21:30: 3000000 INFO @ Tue, 16 Jun 2020 10:21:34: 9000000 INFO @ Tue, 16 Jun 2020 10:21:36: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:21:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:21:40: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:21:40: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:21:41: 10000000 INFO @ Tue, 16 Jun 2020 10:21:44: 5000000 INFO @ Tue, 16 Jun 2020 10:21:48: 1000000 INFO @ Tue, 16 Jun 2020 10:21:48: 11000000 INFO @ Tue, 16 Jun 2020 10:21:50: 6000000 INFO @ Tue, 16 Jun 2020 10:21:55: 12000000 INFO @ Tue, 16 Jun 2020 10:21:55: 2000000 INFO @ Tue, 16 Jun 2020 10:21:57: 7000000 INFO @ Tue, 16 Jun 2020 10:22:01: 13000000 INFO @ Tue, 16 Jun 2020 10:22:02: 3000000 INFO @ Tue, 16 Jun 2020 10:22:03: 8000000 INFO @ Tue, 16 Jun 2020 10:22:08: 14000000 INFO @ Tue, 16 Jun 2020 10:22:10: 9000000 INFO @ Tue, 16 Jun 2020 10:22:10: 4000000 INFO @ Tue, 16 Jun 2020 10:22:14: 15000000 INFO @ Tue, 16 Jun 2020 10:22:16: 10000000 INFO @ Tue, 16 Jun 2020 10:22:17: 5000000 INFO @ Tue, 16 Jun 2020 10:22:21: 16000000 INFO @ Tue, 16 Jun 2020 10:22:23: 11000000 INFO @ Tue, 16 Jun 2020 10:22:24: 6000000 INFO @ Tue, 16 Jun 2020 10:22:27: 17000000 INFO @ Tue, 16 Jun 2020 10:22:30: 12000000 INFO @ Tue, 16 Jun 2020 10:22:32: 7000000 INFO @ Tue, 16 Jun 2020 10:22:34: 18000000 INFO @ Tue, 16 Jun 2020 10:22:36: 13000000 INFO @ Tue, 16 Jun 2020 10:22:39: 8000000 INFO @ Tue, 16 Jun 2020 10:22:41: 19000000 INFO @ Tue, 16 Jun 2020 10:22:43: 14000000 INFO @ Tue, 16 Jun 2020 10:22:46: 9000000 INFO @ Tue, 16 Jun 2020 10:22:47: 20000000 INFO @ Tue, 16 Jun 2020 10:22:50: 15000000 INFO @ Tue, 16 Jun 2020 10:22:53: 10000000 INFO @ Tue, 16 Jun 2020 10:22:54: 21000000 INFO @ Tue, 16 Jun 2020 10:22:56: 16000000 INFO @ Tue, 16 Jun 2020 10:23:00: 11000000 INFO @ Tue, 16 Jun 2020 10:23:01: 22000000 INFO @ Tue, 16 Jun 2020 10:23:03: 17000000 INFO @ Tue, 16 Jun 2020 10:23:07: 12000000 INFO @ Tue, 16 Jun 2020 10:23:08: 23000000 INFO @ Tue, 16 Jun 2020 10:23:10: 18000000 INFO @ Tue, 16 Jun 2020 10:23:14: 13000000 INFO @ Tue, 16 Jun 2020 10:23:14: 24000000 INFO @ Tue, 16 Jun 2020 10:23:16: 19000000 INFO @ Tue, 16 Jun 2020 10:23:21: 25000000 INFO @ Tue, 16 Jun 2020 10:23:21: 14000000 INFO @ Tue, 16 Jun 2020 10:23:23: 20000000 INFO @ Tue, 16 Jun 2020 10:23:28: 26000000 INFO @ Tue, 16 Jun 2020 10:23:28: 15000000 INFO @ Tue, 16 Jun 2020 10:23:29: 21000000 INFO @ Tue, 16 Jun 2020 10:23:34: 27000000 INFO @ Tue, 16 Jun 2020 10:23:35: 16000000 INFO @ Tue, 16 Jun 2020 10:23:35: 22000000 INFO @ Tue, 16 Jun 2020 10:23:40: 28000000 INFO @ Tue, 16 Jun 2020 10:23:42: 23000000 INFO @ Tue, 16 Jun 2020 10:23:42: 17000000 INFO @ Tue, 16 Jun 2020 10:23:47: 29000000 INFO @ Tue, 16 Jun 2020 10:23:48: 24000000 INFO @ Tue, 16 Jun 2020 10:23:49: 18000000 INFO @ Tue, 16 Jun 2020 10:23:53: 30000000 INFO @ Tue, 16 Jun 2020 10:23:55: 25000000 INFO @ Tue, 16 Jun 2020 10:23:56: 19000000 INFO @ Tue, 16 Jun 2020 10:24:00: 31000000 INFO @ Tue, 16 Jun 2020 10:24:01: 26000000 INFO @ Tue, 16 Jun 2020 10:24:03: 20000000 INFO @ Tue, 16 Jun 2020 10:24:06: 32000000 INFO @ Tue, 16 Jun 2020 10:24:07: 27000000 INFO @ Tue, 16 Jun 2020 10:24:10: 21000000 INFO @ Tue, 16 Jun 2020 10:24:13: 33000000 INFO @ Tue, 16 Jun 2020 10:24:14: 28000000 INFO @ Tue, 16 Jun 2020 10:24:16: 22000000 INFO @ Tue, 16 Jun 2020 10:24:19: 34000000 INFO @ Tue, 16 Jun 2020 10:24:20: 29000000 INFO @ Tue, 16 Jun 2020 10:24:23: 23000000 INFO @ Tue, 16 Jun 2020 10:24:25: 35000000 INFO @ Tue, 16 Jun 2020 10:24:27: 30000000 INFO @ Tue, 16 Jun 2020 10:24:30: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:24:32: 36000000 INFO @ Tue, 16 Jun 2020 10:24:33: 31000000 INFO @ Tue, 16 Jun 2020 10:24:37: 25000000 INFO @ Tue, 16 Jun 2020 10:24:38: 37000000 INFO @ Tue, 16 Jun 2020 10:24:39: 32000000 INFO @ Tue, 16 Jun 2020 10:24:44: 26000000 INFO @ Tue, 16 Jun 2020 10:24:44: 38000000 INFO @ Tue, 16 Jun 2020 10:24:46: 33000000 INFO @ Tue, 16 Jun 2020 10:24:50: 39000000 INFO @ Tue, 16 Jun 2020 10:24:51: 27000000 INFO @ Tue, 16 Jun 2020 10:24:52: 34000000 INFO @ Tue, 16 Jun 2020 10:24:53: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:24:53: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:24:53: #1 total tags in treatment: 39361441 INFO @ Tue, 16 Jun 2020 10:24:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:24:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:24:53: #1 tags after filtering in treatment: 39361441 INFO @ Tue, 16 Jun 2020 10:24:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:24:53: #1 finished! INFO @ Tue, 16 Jun 2020 10:24:53: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:24:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:24:56: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:24:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:24:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:24:57: 28000000 INFO @ Tue, 16 Jun 2020 10:24:58: 35000000 INFO @ Tue, 16 Jun 2020 10:25:04: 29000000 INFO @ Tue, 16 Jun 2020 10:25:04: 36000000 INFO @ Tue, 16 Jun 2020 10:25:10: 37000000 INFO @ Tue, 16 Jun 2020 10:25:11: 30000000 INFO @ Tue, 16 Jun 2020 10:25:16: 38000000 INFO @ Tue, 16 Jun 2020 10:25:18: 31000000 INFO @ Tue, 16 Jun 2020 10:25:22: 39000000 INFO @ Tue, 16 Jun 2020 10:25:24: 32000000 INFO @ Tue, 16 Jun 2020 10:25:25: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:25:25: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:25:25: #1 total tags in treatment: 39361441 INFO @ Tue, 16 Jun 2020 10:25:25: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:25:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:25:26: #1 tags after filtering in treatment: 39361441 INFO @ Tue, 16 Jun 2020 10:25:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:25:26: #1 finished! INFO @ Tue, 16 Jun 2020 10:25:26: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:25:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:25:28: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:25:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:25:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:25:31: 33000000 INFO @ Tue, 16 Jun 2020 10:25:38: 34000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:25:44: 35000000 INFO @ Tue, 16 Jun 2020 10:25:50: 36000000 INFO @ Tue, 16 Jun 2020 10:25:57: 37000000 INFO @ Tue, 16 Jun 2020 10:26:03: 38000000 INFO @ Tue, 16 Jun 2020 10:26:10: 39000000 INFO @ Tue, 16 Jun 2020 10:26:12: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:26:12: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:26:12: #1 total tags in treatment: 39361441 INFO @ Tue, 16 Jun 2020 10:26:12: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:26:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:26:13: #1 tags after filtering in treatment: 39361441 INFO @ Tue, 16 Jun 2020 10:26:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:26:13: #1 finished! INFO @ Tue, 16 Jun 2020 10:26:13: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:26:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:26:15: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:26:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:26:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971808/SRX7971808.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling