Job ID = 6368981 SRX = SRX7971806 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:41:19 prefetch.2.10.7: 1) Downloading 'SRR11392692'... 2020-06-16T00:41:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:51:05 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:51:05 prefetch.2.10.7: 1) 'SRR11392692' was downloaded successfully Read 41470633 spots for SRR11392692/SRR11392692.sra Written 41470633 spots for SRR11392692/SRR11392692.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:55 41470633 reads; of these: 41470633 (100.00%) were unpaired; of these: 363645 (0.88%) aligned 0 times 34390976 (82.93%) aligned exactly 1 time 6716012 (16.19%) aligned >1 times 99.12% overall alignment rate Time searching: 00:13:56 Overall time: 00:13:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 6200885 / 41106988 = 0.1508 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:18:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:18:48: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:18:48: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:18:54: 1000000 INFO @ Tue, 16 Jun 2020 10:19:00: 2000000 INFO @ Tue, 16 Jun 2020 10:19:06: 3000000 INFO @ Tue, 16 Jun 2020 10:19:12: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:19:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:19:18: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:19:18: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:19:18: 5000000 INFO @ Tue, 16 Jun 2020 10:19:24: 6000000 INFO @ Tue, 16 Jun 2020 10:19:25: 1000000 INFO @ Tue, 16 Jun 2020 10:19:30: 7000000 INFO @ Tue, 16 Jun 2020 10:19:32: 2000000 INFO @ Tue, 16 Jun 2020 10:19:36: 8000000 INFO @ Tue, 16 Jun 2020 10:19:39: 3000000 INFO @ Tue, 16 Jun 2020 10:19:42: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:19:46: 4000000 INFO @ Tue, 16 Jun 2020 10:19:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:19:48: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:19:48: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:19:48: 10000000 INFO @ Tue, 16 Jun 2020 10:19:53: 5000000 INFO @ Tue, 16 Jun 2020 10:19:54: 11000000 INFO @ Tue, 16 Jun 2020 10:19:55: 1000000 INFO @ Tue, 16 Jun 2020 10:20:00: 6000000 INFO @ Tue, 16 Jun 2020 10:20:01: 12000000 INFO @ Tue, 16 Jun 2020 10:20:02: 2000000 INFO @ Tue, 16 Jun 2020 10:20:07: 7000000 INFO @ Tue, 16 Jun 2020 10:20:07: 13000000 INFO @ Tue, 16 Jun 2020 10:20:09: 3000000 INFO @ Tue, 16 Jun 2020 10:20:13: 14000000 INFO @ Tue, 16 Jun 2020 10:20:13: 8000000 INFO @ Tue, 16 Jun 2020 10:20:16: 4000000 INFO @ Tue, 16 Jun 2020 10:20:19: 15000000 INFO @ Tue, 16 Jun 2020 10:20:20: 9000000 INFO @ Tue, 16 Jun 2020 10:20:23: 5000000 INFO @ Tue, 16 Jun 2020 10:20:26: 16000000 INFO @ Tue, 16 Jun 2020 10:20:27: 10000000 INFO @ Tue, 16 Jun 2020 10:20:30: 6000000 INFO @ Tue, 16 Jun 2020 10:20:32: 17000000 INFO @ Tue, 16 Jun 2020 10:20:34: 11000000 INFO @ Tue, 16 Jun 2020 10:20:37: 7000000 INFO @ Tue, 16 Jun 2020 10:20:39: 18000000 INFO @ Tue, 16 Jun 2020 10:20:41: 12000000 INFO @ Tue, 16 Jun 2020 10:20:44: 8000000 INFO @ Tue, 16 Jun 2020 10:20:45: 19000000 INFO @ Tue, 16 Jun 2020 10:20:48: 13000000 INFO @ Tue, 16 Jun 2020 10:20:50: 9000000 INFO @ Tue, 16 Jun 2020 10:20:51: 20000000 INFO @ Tue, 16 Jun 2020 10:20:55: 14000000 INFO @ Tue, 16 Jun 2020 10:20:57: 10000000 INFO @ Tue, 16 Jun 2020 10:20:58: 21000000 INFO @ Tue, 16 Jun 2020 10:21:02: 15000000 INFO @ Tue, 16 Jun 2020 10:21:04: 22000000 INFO @ Tue, 16 Jun 2020 10:21:04: 11000000 INFO @ Tue, 16 Jun 2020 10:21:10: 16000000 INFO @ Tue, 16 Jun 2020 10:21:10: 23000000 INFO @ Tue, 16 Jun 2020 10:21:12: 12000000 INFO @ Tue, 16 Jun 2020 10:21:16: 17000000 INFO @ Tue, 16 Jun 2020 10:21:17: 24000000 INFO @ Tue, 16 Jun 2020 10:21:19: 13000000 INFO @ Tue, 16 Jun 2020 10:21:23: 18000000 INFO @ Tue, 16 Jun 2020 10:21:23: 25000000 INFO @ Tue, 16 Jun 2020 10:21:26: 14000000 INFO @ Tue, 16 Jun 2020 10:21:29: 26000000 INFO @ Tue, 16 Jun 2020 10:21:30: 19000000 INFO @ Tue, 16 Jun 2020 10:21:34: 15000000 INFO @ Tue, 16 Jun 2020 10:21:36: 27000000 INFO @ Tue, 16 Jun 2020 10:21:37: 20000000 INFO @ Tue, 16 Jun 2020 10:21:41: 16000000 INFO @ Tue, 16 Jun 2020 10:21:42: 28000000 INFO @ Tue, 16 Jun 2020 10:21:44: 21000000 INFO @ Tue, 16 Jun 2020 10:21:48: 17000000 INFO @ Tue, 16 Jun 2020 10:21:49: 29000000 INFO @ Tue, 16 Jun 2020 10:21:51: 22000000 INFO @ Tue, 16 Jun 2020 10:21:55: 18000000 INFO @ Tue, 16 Jun 2020 10:21:55: 30000000 INFO @ Tue, 16 Jun 2020 10:21:58: 23000000 INFO @ Tue, 16 Jun 2020 10:22:01: 31000000 INFO @ Tue, 16 Jun 2020 10:22:02: 19000000 INFO @ Tue, 16 Jun 2020 10:22:06: 24000000 INFO @ Tue, 16 Jun 2020 10:22:08: 32000000 INFO @ Tue, 16 Jun 2020 10:22:10: 20000000 INFO @ Tue, 16 Jun 2020 10:22:13: 25000000 INFO @ Tue, 16 Jun 2020 10:22:14: 33000000 INFO @ Tue, 16 Jun 2020 10:22:18: 21000000 INFO @ Tue, 16 Jun 2020 10:22:19: 26000000 INFO @ Tue, 16 Jun 2020 10:22:21: 34000000 INFO @ Tue, 16 Jun 2020 10:22:25: 22000000 INFO @ Tue, 16 Jun 2020 10:22:26: 27000000 INFO @ Tue, 16 Jun 2020 10:22:27: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:22:27: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:22:27: #1 total tags in treatment: 34906103 INFO @ Tue, 16 Jun 2020 10:22:27: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:22:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:22:27: #1 tags after filtering in treatment: 34906103 INFO @ Tue, 16 Jun 2020 10:22:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:22:27: #1 finished! INFO @ Tue, 16 Jun 2020 10:22:27: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:22:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:22:30: #2 number of paired peaks: 26 WARNING @ Tue, 16 Jun 2020 10:22:30: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:22:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:22:33: 23000000 INFO @ Tue, 16 Jun 2020 10:22:34: 28000000 INFO @ Tue, 16 Jun 2020 10:22:40: 24000000 INFO @ Tue, 16 Jun 2020 10:22:41: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:22:47: 25000000 INFO @ Tue, 16 Jun 2020 10:22:48: 30000000 INFO @ Tue, 16 Jun 2020 10:22:54: 26000000 INFO @ Tue, 16 Jun 2020 10:22:55: 31000000 INFO @ Tue, 16 Jun 2020 10:23:02: 27000000 INFO @ Tue, 16 Jun 2020 10:23:03: 32000000 INFO @ Tue, 16 Jun 2020 10:23:09: 28000000 INFO @ Tue, 16 Jun 2020 10:23:10: 33000000 INFO @ Tue, 16 Jun 2020 10:23:17: 29000000 INFO @ Tue, 16 Jun 2020 10:23:17: 34000000 INFO @ Tue, 16 Jun 2020 10:23:24: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:23:24: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:23:24: #1 total tags in treatment: 34906103 INFO @ Tue, 16 Jun 2020 10:23:24: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:23:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:23:24: #1 tags after filtering in treatment: 34906103 INFO @ Tue, 16 Jun 2020 10:23:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:23:24: #1 finished! INFO @ Tue, 16 Jun 2020 10:23:24: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:23:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:23:24: 30000000 INFO @ Tue, 16 Jun 2020 10:23:27: #2 number of paired peaks: 26 WARNING @ Tue, 16 Jun 2020 10:23:27: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:23:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:23:32: 31000000 INFO @ Tue, 16 Jun 2020 10:23:39: 32000000 INFO @ Tue, 16 Jun 2020 10:23:47: 33000000 INFO @ Tue, 16 Jun 2020 10:23:54: 34000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:24:00: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:24:00: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:24:00: #1 total tags in treatment: 34906103 INFO @ Tue, 16 Jun 2020 10:24:00: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:24:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:24:01: #1 tags after filtering in treatment: 34906103 INFO @ Tue, 16 Jun 2020 10:24:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:24:01: #1 finished! INFO @ Tue, 16 Jun 2020 10:24:01: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:24:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:24:03: #2 number of paired peaks: 26 WARNING @ Tue, 16 Jun 2020 10:24:03: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:24:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971806/SRX7971806.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling