Job ID = 6368975 SRX = SRX7971800 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:43:31 prefetch.2.10.7: 1) Downloading 'SRR11392686'... 2020-06-16T00:43:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:50:15 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:50:15 prefetch.2.10.7: 1) 'SRR11392686' was downloaded successfully Read 32864387 spots for SRR11392686/SRR11392686.sra Written 32864387 spots for SRR11392686/SRR11392686.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:10:44 32864387 reads; of these: 32864387 (100.00%) were unpaired; of these: 701565 (2.13%) aligned 0 times 26966649 (82.05%) aligned exactly 1 time 5196173 (15.81%) aligned >1 times 97.87% overall alignment rate Time searching: 00:10:45 Overall time: 00:10:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4084753 / 32162822 = 0.1270 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:14:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:14:49: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:14:49: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:14:57: 1000000 INFO @ Tue, 16 Jun 2020 10:15:05: 2000000 INFO @ Tue, 16 Jun 2020 10:15:14: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:15:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:15:18: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:15:18: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:15:23: 4000000 INFO @ Tue, 16 Jun 2020 10:15:27: 1000000 INFO @ Tue, 16 Jun 2020 10:15:32: 5000000 INFO @ Tue, 16 Jun 2020 10:15:36: 2000000 INFO @ Tue, 16 Jun 2020 10:15:41: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:15:46: 3000000 INFO @ Tue, 16 Jun 2020 10:15:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:15:47: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:15:47: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:15:51: 7000000 INFO @ Tue, 16 Jun 2020 10:15:55: 4000000 INFO @ Tue, 16 Jun 2020 10:15:56: 1000000 INFO @ Tue, 16 Jun 2020 10:16:00: 8000000 INFO @ Tue, 16 Jun 2020 10:16:05: 5000000 INFO @ Tue, 16 Jun 2020 10:16:05: 2000000 INFO @ Tue, 16 Jun 2020 10:16:09: 9000000 INFO @ Tue, 16 Jun 2020 10:16:14: 3000000 INFO @ Tue, 16 Jun 2020 10:16:14: 6000000 INFO @ Tue, 16 Jun 2020 10:16:18: 10000000 INFO @ Tue, 16 Jun 2020 10:16:23: 4000000 INFO @ Tue, 16 Jun 2020 10:16:24: 7000000 INFO @ Tue, 16 Jun 2020 10:16:28: 11000000 INFO @ Tue, 16 Jun 2020 10:16:32: 5000000 INFO @ Tue, 16 Jun 2020 10:16:33: 8000000 INFO @ Tue, 16 Jun 2020 10:16:37: 12000000 INFO @ Tue, 16 Jun 2020 10:16:40: 6000000 INFO @ Tue, 16 Jun 2020 10:16:43: 9000000 INFO @ Tue, 16 Jun 2020 10:16:47: 13000000 INFO @ Tue, 16 Jun 2020 10:16:49: 7000000 INFO @ Tue, 16 Jun 2020 10:16:52: 10000000 INFO @ Tue, 16 Jun 2020 10:16:56: 14000000 INFO @ Tue, 16 Jun 2020 10:16:58: 8000000 INFO @ Tue, 16 Jun 2020 10:17:01: 11000000 INFO @ Tue, 16 Jun 2020 10:17:05: 15000000 INFO @ Tue, 16 Jun 2020 10:17:06: 9000000 INFO @ Tue, 16 Jun 2020 10:17:10: 12000000 INFO @ Tue, 16 Jun 2020 10:17:15: 10000000 INFO @ Tue, 16 Jun 2020 10:17:15: 16000000 INFO @ Tue, 16 Jun 2020 10:17:20: 13000000 INFO @ Tue, 16 Jun 2020 10:17:23: 11000000 INFO @ Tue, 16 Jun 2020 10:17:24: 17000000 INFO @ Tue, 16 Jun 2020 10:17:29: 14000000 INFO @ Tue, 16 Jun 2020 10:17:31: 12000000 INFO @ Tue, 16 Jun 2020 10:17:32: 18000000 INFO @ Tue, 16 Jun 2020 10:17:37: 15000000 INFO @ Tue, 16 Jun 2020 10:17:39: 13000000 INFO @ Tue, 16 Jun 2020 10:17:40: 19000000 INFO @ Tue, 16 Jun 2020 10:17:46: 16000000 INFO @ Tue, 16 Jun 2020 10:17:48: 14000000 INFO @ Tue, 16 Jun 2020 10:17:48: 20000000 INFO @ Tue, 16 Jun 2020 10:17:54: 17000000 INFO @ Tue, 16 Jun 2020 10:17:56: 15000000 INFO @ Tue, 16 Jun 2020 10:17:56: 21000000 INFO @ Tue, 16 Jun 2020 10:18:02: 18000000 INFO @ Tue, 16 Jun 2020 10:18:05: 22000000 INFO @ Tue, 16 Jun 2020 10:18:05: 16000000 INFO @ Tue, 16 Jun 2020 10:18:11: 19000000 INFO @ Tue, 16 Jun 2020 10:18:13: 23000000 INFO @ Tue, 16 Jun 2020 10:18:15: 17000000 INFO @ Tue, 16 Jun 2020 10:18:19: 20000000 INFO @ Tue, 16 Jun 2020 10:18:21: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:18:24: 18000000 INFO @ Tue, 16 Jun 2020 10:18:28: 21000000 INFO @ Tue, 16 Jun 2020 10:18:30: 25000000 INFO @ Tue, 16 Jun 2020 10:18:33: 19000000 INFO @ Tue, 16 Jun 2020 10:18:36: 22000000 INFO @ Tue, 16 Jun 2020 10:18:38: 26000000 INFO @ Tue, 16 Jun 2020 10:18:42: 20000000 INFO @ Tue, 16 Jun 2020 10:18:44: 23000000 INFO @ Tue, 16 Jun 2020 10:18:46: 27000000 INFO @ Tue, 16 Jun 2020 10:18:51: 21000000 INFO @ Tue, 16 Jun 2020 10:18:53: 24000000 INFO @ Tue, 16 Jun 2020 10:18:54: 28000000 INFO @ Tue, 16 Jun 2020 10:18:55: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:18:55: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:18:55: #1 total tags in treatment: 28078069 INFO @ Tue, 16 Jun 2020 10:18:55: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:18:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:18:56: #1 tags after filtering in treatment: 28078069 INFO @ Tue, 16 Jun 2020 10:18:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:18:56: #1 finished! INFO @ Tue, 16 Jun 2020 10:18:56: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:18:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:18:57: #2 number of paired peaks: 90 WARNING @ Tue, 16 Jun 2020 10:18:57: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:18:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:19:00: 22000000 INFO @ Tue, 16 Jun 2020 10:19:01: 25000000 INFO @ Tue, 16 Jun 2020 10:19:09: 23000000 INFO @ Tue, 16 Jun 2020 10:19:09: 26000000 INFO @ Tue, 16 Jun 2020 10:19:17: 27000000 INFO @ Tue, 16 Jun 2020 10:19:18: 24000000 INFO @ Tue, 16 Jun 2020 10:19:26: 28000000 INFO @ Tue, 16 Jun 2020 10:19:27: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:19:27: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:19:27: #1 total tags in treatment: 28078069 INFO @ Tue, 16 Jun 2020 10:19:27: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:19:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:19:27: #1 tags after filtering in treatment: 28078069 INFO @ Tue, 16 Jun 2020 10:19:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:19:27: #1 finished! INFO @ Tue, 16 Jun 2020 10:19:27: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:19:27: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:19:27: 25000000 INFO @ Tue, 16 Jun 2020 10:19:29: #2 number of paired peaks: 90 WARNING @ Tue, 16 Jun 2020 10:19:29: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:19:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:19:36: 26000000 INFO @ Tue, 16 Jun 2020 10:19:44: 27000000 INFO @ Tue, 16 Jun 2020 10:19:52: 28000000 INFO @ Tue, 16 Jun 2020 10:19:53: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:19:53: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:19:53: #1 total tags in treatment: 28078069 INFO @ Tue, 16 Jun 2020 10:19:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:19:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:19:53: #1 tags after filtering in treatment: 28078069 INFO @ Tue, 16 Jun 2020 10:19:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:19:53: #1 finished! INFO @ Tue, 16 Jun 2020 10:19:53: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:19:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:19:55: #2 number of paired peaks: 90 WARNING @ Tue, 16 Jun 2020 10:19:55: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:19:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971800/SRX7971800.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling