Job ID = 6368973 SRX = SRX7971798 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:36:35 prefetch.2.10.7: 1) Downloading 'SRR11392684'... 2020-06-16T00:36:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:41:30 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:41:30 prefetch.2.10.7: 1) 'SRR11392684' was downloaded successfully Read 35864156 spots for SRR11392684/SRR11392684.sra Written 35864156 spots for SRR11392684/SRR11392684.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:55 35864156 reads; of these: 35864156 (100.00%) were unpaired; of these: 831613 (2.32%) aligned 0 times 29335344 (81.80%) aligned exactly 1 time 5697199 (15.89%) aligned >1 times 97.68% overall alignment rate Time searching: 00:11:55 Overall time: 00:11:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4757456 / 35032543 = 0.1358 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:08:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:08:23: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:08:23: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:08:30: 1000000 INFO @ Tue, 16 Jun 2020 10:08:37: 2000000 INFO @ Tue, 16 Jun 2020 10:08:44: 3000000 INFO @ Tue, 16 Jun 2020 10:08:50: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:08:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:08:53: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:08:53: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:08:58: 5000000 INFO @ Tue, 16 Jun 2020 10:09:01: 1000000 INFO @ Tue, 16 Jun 2020 10:09:06: 6000000 INFO @ Tue, 16 Jun 2020 10:09:08: 2000000 INFO @ Tue, 16 Jun 2020 10:09:14: 7000000 INFO @ Tue, 16 Jun 2020 10:09:16: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:09:22: 8000000 INFO @ Tue, 16 Jun 2020 10:09:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:09:23: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:09:23: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:09:23: 4000000 INFO @ Tue, 16 Jun 2020 10:09:30: 1000000 INFO @ Tue, 16 Jun 2020 10:09:31: 9000000 INFO @ Tue, 16 Jun 2020 10:09:31: 5000000 INFO @ Tue, 16 Jun 2020 10:09:38: 2000000 INFO @ Tue, 16 Jun 2020 10:09:39: 6000000 INFO @ Tue, 16 Jun 2020 10:09:39: 10000000 INFO @ Tue, 16 Jun 2020 10:09:46: 3000000 INFO @ Tue, 16 Jun 2020 10:09:47: 7000000 INFO @ Tue, 16 Jun 2020 10:09:48: 11000000 INFO @ Tue, 16 Jun 2020 10:09:54: 4000000 INFO @ Tue, 16 Jun 2020 10:09:56: 8000000 INFO @ Tue, 16 Jun 2020 10:09:57: 12000000 INFO @ Tue, 16 Jun 2020 10:10:01: 5000000 INFO @ Tue, 16 Jun 2020 10:10:04: 9000000 INFO @ Tue, 16 Jun 2020 10:10:05: 13000000 INFO @ Tue, 16 Jun 2020 10:10:10: 6000000 INFO @ Tue, 16 Jun 2020 10:10:13: 10000000 INFO @ Tue, 16 Jun 2020 10:10:14: 14000000 INFO @ Tue, 16 Jun 2020 10:10:18: 7000000 INFO @ Tue, 16 Jun 2020 10:10:21: 11000000 INFO @ Tue, 16 Jun 2020 10:10:23: 15000000 INFO @ Tue, 16 Jun 2020 10:10:27: 8000000 INFO @ Tue, 16 Jun 2020 10:10:30: 12000000 INFO @ Tue, 16 Jun 2020 10:10:32: 16000000 INFO @ Tue, 16 Jun 2020 10:10:35: 9000000 INFO @ Tue, 16 Jun 2020 10:10:38: 13000000 INFO @ Tue, 16 Jun 2020 10:10:40: 17000000 INFO @ Tue, 16 Jun 2020 10:10:44: 10000000 INFO @ Tue, 16 Jun 2020 10:10:47: 14000000 INFO @ Tue, 16 Jun 2020 10:10:49: 18000000 INFO @ Tue, 16 Jun 2020 10:10:53: 11000000 INFO @ Tue, 16 Jun 2020 10:10:56: 15000000 INFO @ Tue, 16 Jun 2020 10:10:57: 19000000 INFO @ Tue, 16 Jun 2020 10:11:01: 12000000 INFO @ Tue, 16 Jun 2020 10:11:04: 16000000 INFO @ Tue, 16 Jun 2020 10:11:06: 20000000 INFO @ Tue, 16 Jun 2020 10:11:10: 13000000 INFO @ Tue, 16 Jun 2020 10:11:13: 17000000 INFO @ Tue, 16 Jun 2020 10:11:14: 21000000 INFO @ Tue, 16 Jun 2020 10:11:18: 14000000 INFO @ Tue, 16 Jun 2020 10:11:22: 18000000 INFO @ Tue, 16 Jun 2020 10:11:23: 22000000 INFO @ Tue, 16 Jun 2020 10:11:27: 15000000 INFO @ Tue, 16 Jun 2020 10:11:30: 19000000 INFO @ Tue, 16 Jun 2020 10:11:31: 23000000 INFO @ Tue, 16 Jun 2020 10:11:35: 16000000 INFO @ Tue, 16 Jun 2020 10:11:39: 20000000 INFO @ Tue, 16 Jun 2020 10:11:40: 24000000 INFO @ Tue, 16 Jun 2020 10:11:44: 17000000 INFO @ Tue, 16 Jun 2020 10:11:48: 21000000 INFO @ Tue, 16 Jun 2020 10:11:48: 25000000 INFO @ Tue, 16 Jun 2020 10:11:52: 18000000 INFO @ Tue, 16 Jun 2020 10:11:56: 22000000 INFO @ Tue, 16 Jun 2020 10:11:57: 26000000 INFO @ Tue, 16 Jun 2020 10:12:01: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:12:05: 23000000 INFO @ Tue, 16 Jun 2020 10:12:05: 27000000 INFO @ Tue, 16 Jun 2020 10:12:09: 20000000 INFO @ Tue, 16 Jun 2020 10:12:13: 24000000 INFO @ Tue, 16 Jun 2020 10:12:14: 28000000 INFO @ Tue, 16 Jun 2020 10:12:17: 21000000 INFO @ Tue, 16 Jun 2020 10:12:22: 25000000 INFO @ Tue, 16 Jun 2020 10:12:23: 29000000 INFO @ Tue, 16 Jun 2020 10:12:26: 22000000 INFO @ Tue, 16 Jun 2020 10:12:30: 26000000 INFO @ Tue, 16 Jun 2020 10:12:32: 30000000 INFO @ Tue, 16 Jun 2020 10:12:34: 23000000 INFO @ Tue, 16 Jun 2020 10:12:34: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:12:34: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:12:34: #1 total tags in treatment: 30275087 INFO @ Tue, 16 Jun 2020 10:12:34: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:12:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:12:35: #1 tags after filtering in treatment: 30275087 INFO @ Tue, 16 Jun 2020 10:12:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:12:35: #1 finished! INFO @ Tue, 16 Jun 2020 10:12:35: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:12:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:12:37: #2 number of paired peaks: 72 WARNING @ Tue, 16 Jun 2020 10:12:37: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:12:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:12:39: 27000000 INFO @ Tue, 16 Jun 2020 10:12:42: 24000000 INFO @ Tue, 16 Jun 2020 10:12:47: 28000000 INFO @ Tue, 16 Jun 2020 10:12:51: 25000000 INFO @ Tue, 16 Jun 2020 10:12:55: 29000000 INFO @ Tue, 16 Jun 2020 10:12:59: 26000000 INFO @ Tue, 16 Jun 2020 10:13:04: 30000000 INFO @ Tue, 16 Jun 2020 10:13:06: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:13:06: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:13:06: #1 total tags in treatment: 30275087 INFO @ Tue, 16 Jun 2020 10:13:06: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:13:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:13:07: #1 tags after filtering in treatment: 30275087 INFO @ Tue, 16 Jun 2020 10:13:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:13:07: #1 finished! INFO @ Tue, 16 Jun 2020 10:13:07: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:13:07: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:13:08: 27000000 INFO @ Tue, 16 Jun 2020 10:13:09: #2 number of paired peaks: 72 WARNING @ Tue, 16 Jun 2020 10:13:09: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:13:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:13:16: 28000000 INFO @ Tue, 16 Jun 2020 10:13:24: 29000000 INFO @ Tue, 16 Jun 2020 10:13:32: 30000000 INFO @ Tue, 16 Jun 2020 10:13:34: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:13:34: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:13:34: #1 total tags in treatment: 30275087 INFO @ Tue, 16 Jun 2020 10:13:34: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:13:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:13:35: #1 tags after filtering in treatment: 30275087 INFO @ Tue, 16 Jun 2020 10:13:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:13:35: #1 finished! INFO @ Tue, 16 Jun 2020 10:13:35: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:13:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:13:37: #2 number of paired peaks: 72 WARNING @ Tue, 16 Jun 2020 10:13:37: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:13:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971798/SRX7971798.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling