Job ID = 6368971 SRX = SRX7971796 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:33:43 prefetch.2.10.7: 1) Downloading 'SRR11392682'... 2020-06-16T00:33:43 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:40:33 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:40:33 prefetch.2.10.7: 1) 'SRR11392682' was downloaded successfully Read 24827639 spots for SRR11392682/SRR11392682.sra Written 24827639 spots for SRR11392682/SRR11392682.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:52 24827639 reads; of these: 24827639 (100.00%) were unpaired; of these: 433829 (1.75%) aligned 0 times 21959373 (88.45%) aligned exactly 1 time 2434437 (9.81%) aligned >1 times 98.25% overall alignment rate Time searching: 00:07:52 Overall time: 00:07:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3979233 / 24393810 = 0.1631 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:58:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:58:28: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:58:28: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:58:35: 1000000 INFO @ Tue, 16 Jun 2020 09:58:41: 2000000 INFO @ Tue, 16 Jun 2020 09:58:48: 3000000 INFO @ Tue, 16 Jun 2020 09:58:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:58:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:58:58: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:58:58: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:59:01: 5000000 INFO @ Tue, 16 Jun 2020 09:59:06: 1000000 INFO @ Tue, 16 Jun 2020 09:59:08: 6000000 INFO @ Tue, 16 Jun 2020 09:59:13: 2000000 INFO @ Tue, 16 Jun 2020 09:59:16: 7000000 INFO @ Tue, 16 Jun 2020 09:59:21: 3000000 INFO @ Tue, 16 Jun 2020 09:59:23: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:59:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:59:28: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:59:28: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:59:28: 4000000 INFO @ Tue, 16 Jun 2020 09:59:31: 9000000 INFO @ Tue, 16 Jun 2020 09:59:36: 5000000 INFO @ Tue, 16 Jun 2020 09:59:36: 1000000 INFO @ Tue, 16 Jun 2020 09:59:39: 10000000 INFO @ Tue, 16 Jun 2020 09:59:43: 2000000 INFO @ Tue, 16 Jun 2020 09:59:43: 6000000 INFO @ Tue, 16 Jun 2020 09:59:46: 11000000 INFO @ Tue, 16 Jun 2020 09:59:51: 3000000 INFO @ Tue, 16 Jun 2020 09:59:51: 7000000 INFO @ Tue, 16 Jun 2020 09:59:54: 12000000 INFO @ Tue, 16 Jun 2020 09:59:58: 4000000 INFO @ Tue, 16 Jun 2020 09:59:59: 8000000 INFO @ Tue, 16 Jun 2020 10:00:01: 13000000 INFO @ Tue, 16 Jun 2020 10:00:06: 5000000 INFO @ Tue, 16 Jun 2020 10:00:06: 9000000 INFO @ Tue, 16 Jun 2020 10:00:09: 14000000 INFO @ Tue, 16 Jun 2020 10:00:13: 6000000 INFO @ Tue, 16 Jun 2020 10:00:14: 10000000 INFO @ Tue, 16 Jun 2020 10:00:17: 15000000 INFO @ Tue, 16 Jun 2020 10:00:21: 7000000 INFO @ Tue, 16 Jun 2020 10:00:21: 11000000 INFO @ Tue, 16 Jun 2020 10:00:24: 16000000 INFO @ Tue, 16 Jun 2020 10:00:28: 8000000 INFO @ Tue, 16 Jun 2020 10:00:29: 12000000 INFO @ Tue, 16 Jun 2020 10:00:32: 17000000 INFO @ Tue, 16 Jun 2020 10:00:36: 9000000 INFO @ Tue, 16 Jun 2020 10:00:37: 13000000 INFO @ Tue, 16 Jun 2020 10:00:40: 18000000 INFO @ Tue, 16 Jun 2020 10:00:44: 10000000 INFO @ Tue, 16 Jun 2020 10:00:44: 14000000 INFO @ Tue, 16 Jun 2020 10:00:47: 19000000 INFO @ Tue, 16 Jun 2020 10:00:51: 11000000 INFO @ Tue, 16 Jun 2020 10:00:52: 15000000 INFO @ Tue, 16 Jun 2020 10:00:55: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:00:58: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:00:58: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:00:58: #1 total tags in treatment: 20414577 INFO @ Tue, 16 Jun 2020 10:00:58: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:00:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:00:58: #1 tags after filtering in treatment: 20414577 INFO @ Tue, 16 Jun 2020 10:00:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:00:58: #1 finished! INFO @ Tue, 16 Jun 2020 10:00:58: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:00:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:00:59: 12000000 INFO @ Tue, 16 Jun 2020 10:01:00: 16000000 INFO @ Tue, 16 Jun 2020 10:01:00: #2 number of paired peaks: 53 WARNING @ Tue, 16 Jun 2020 10:01:00: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:01:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:01:06: 13000000 INFO @ Tue, 16 Jun 2020 10:01:07: 17000000 INFO @ Tue, 16 Jun 2020 10:01:14: 14000000 INFO @ Tue, 16 Jun 2020 10:01:15: 18000000 INFO @ Tue, 16 Jun 2020 10:01:21: 15000000 INFO @ Tue, 16 Jun 2020 10:01:22: 19000000 INFO @ Tue, 16 Jun 2020 10:01:29: 16000000 INFO @ Tue, 16 Jun 2020 10:01:30: 20000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:01:33: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:01:33: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:01:33: #1 total tags in treatment: 20414577 INFO @ Tue, 16 Jun 2020 10:01:33: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:01:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:01:33: #1 tags after filtering in treatment: 20414577 INFO @ Tue, 16 Jun 2020 10:01:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:01:33: #1 finished! INFO @ Tue, 16 Jun 2020 10:01:33: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:01:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:01:35: #2 number of paired peaks: 53 WARNING @ Tue, 16 Jun 2020 10:01:35: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:01:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:01:36: 17000000 INFO @ Tue, 16 Jun 2020 10:01:43: 18000000 INFO @ Tue, 16 Jun 2020 10:01:49: 19000000 INFO @ Tue, 16 Jun 2020 10:01:56: 20000000 INFO @ Tue, 16 Jun 2020 10:01:58: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:01:58: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:01:58: #1 total tags in treatment: 20414577 INFO @ Tue, 16 Jun 2020 10:01:58: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:01:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:01:59: #1 tags after filtering in treatment: 20414577 INFO @ Tue, 16 Jun 2020 10:01:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:01:59: #1 finished! INFO @ Tue, 16 Jun 2020 10:01:59: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:01:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:02:00: #2 number of paired peaks: 53 WARNING @ Tue, 16 Jun 2020 10:02:00: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:02:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971796/SRX7971796.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling