Job ID = 6368967 SRX = SRX7971793 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:32:30 prefetch.2.10.7: 1) Downloading 'SRR11392679'... 2020-06-16T00:32:30 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:35:47 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:35:47 prefetch.2.10.7: 1) 'SRR11392679' was downloaded successfully 2020-06-16T00:35:47 prefetch.2.10.7: 'SRR11392679' has 0 unresolved dependencies Read 24304532 spots for SRR11392679/SRR11392679.sra Written 24304532 spots for SRR11392679/SRR11392679.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:03 24304532 reads; of these: 24304532 (100.00%) were unpaired; of these: 125637 (0.52%) aligned 0 times 21799655 (89.69%) aligned exactly 1 time 2379240 (9.79%) aligned >1 times 99.48% overall alignment rate Time searching: 00:08:03 Overall time: 00:08:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4198548 / 24178895 = 0.1736 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:51:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:51:27: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:51:27: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:51:34: 1000000 INFO @ Tue, 16 Jun 2020 09:51:41: 2000000 INFO @ Tue, 16 Jun 2020 09:51:48: 3000000 INFO @ Tue, 16 Jun 2020 09:51:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:51:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:51:57: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:51:57: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:52:02: 5000000 INFO @ Tue, 16 Jun 2020 09:52:04: 1000000 INFO @ Tue, 16 Jun 2020 09:52:09: 6000000 INFO @ Tue, 16 Jun 2020 09:52:11: 2000000 INFO @ Tue, 16 Jun 2020 09:52:16: 7000000 INFO @ Tue, 16 Jun 2020 09:52:18: 3000000 INFO @ Tue, 16 Jun 2020 09:52:23: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:52:25: 4000000 INFO @ Tue, 16 Jun 2020 09:52:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:52:27: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:52:27: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:52:30: 9000000 INFO @ Tue, 16 Jun 2020 09:52:32: 5000000 INFO @ Tue, 16 Jun 2020 09:52:33: 1000000 INFO @ Tue, 16 Jun 2020 09:52:37: 10000000 INFO @ Tue, 16 Jun 2020 09:52:39: 6000000 INFO @ Tue, 16 Jun 2020 09:52:39: 2000000 INFO @ Tue, 16 Jun 2020 09:52:44: 11000000 INFO @ Tue, 16 Jun 2020 09:52:46: 3000000 INFO @ Tue, 16 Jun 2020 09:52:46: 7000000 INFO @ Tue, 16 Jun 2020 09:52:51: 12000000 INFO @ Tue, 16 Jun 2020 09:52:52: 4000000 INFO @ Tue, 16 Jun 2020 09:52:53: 8000000 INFO @ Tue, 16 Jun 2020 09:52:58: 13000000 INFO @ Tue, 16 Jun 2020 09:52:58: 5000000 INFO @ Tue, 16 Jun 2020 09:53:00: 9000000 INFO @ Tue, 16 Jun 2020 09:53:04: 6000000 INFO @ Tue, 16 Jun 2020 09:53:05: 14000000 INFO @ Tue, 16 Jun 2020 09:53:07: 10000000 INFO @ Tue, 16 Jun 2020 09:53:10: 7000000 INFO @ Tue, 16 Jun 2020 09:53:12: 15000000 INFO @ Tue, 16 Jun 2020 09:53:14: 11000000 INFO @ Tue, 16 Jun 2020 09:53:17: 8000000 INFO @ Tue, 16 Jun 2020 09:53:19: 16000000 INFO @ Tue, 16 Jun 2020 09:53:21: 12000000 INFO @ Tue, 16 Jun 2020 09:53:23: 9000000 INFO @ Tue, 16 Jun 2020 09:53:26: 17000000 INFO @ Tue, 16 Jun 2020 09:53:28: 13000000 INFO @ Tue, 16 Jun 2020 09:53:29: 10000000 INFO @ Tue, 16 Jun 2020 09:53:33: 18000000 INFO @ Tue, 16 Jun 2020 09:53:35: 11000000 INFO @ Tue, 16 Jun 2020 09:53:35: 14000000 INFO @ Tue, 16 Jun 2020 09:53:40: 19000000 INFO @ Tue, 16 Jun 2020 09:53:42: 12000000 INFO @ Tue, 16 Jun 2020 09:53:43: 15000000 INFO @ Tue, 16 Jun 2020 09:53:47: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:53:47: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:53:47: #1 total tags in treatment: 19980347 INFO @ Tue, 16 Jun 2020 09:53:47: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:53:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:53:47: #1 tags after filtering in treatment: 19980347 INFO @ Tue, 16 Jun 2020 09:53:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:53:47: #1 finished! INFO @ Tue, 16 Jun 2020 09:53:47: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:53:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:53:48: 13000000 INFO @ Tue, 16 Jun 2020 09:53:49: #2 number of paired peaks: 54 WARNING @ Tue, 16 Jun 2020 09:53:49: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:53:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:53:50: 16000000 INFO @ Tue, 16 Jun 2020 09:53:54: 14000000 INFO @ Tue, 16 Jun 2020 09:53:57: 17000000 INFO @ Tue, 16 Jun 2020 09:54:00: 15000000 INFO @ Tue, 16 Jun 2020 09:54:04: 18000000 INFO @ Tue, 16 Jun 2020 09:54:07: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:54:11: 19000000 INFO @ Tue, 16 Jun 2020 09:54:13: 17000000 INFO @ Tue, 16 Jun 2020 09:54:18: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:54:18: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:54:18: #1 total tags in treatment: 19980347 INFO @ Tue, 16 Jun 2020 09:54:18: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:54:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:54:19: #1 tags after filtering in treatment: 19980347 INFO @ Tue, 16 Jun 2020 09:54:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:54:19: #1 finished! INFO @ Tue, 16 Jun 2020 09:54:19: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:54:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:54:19: 18000000 INFO @ Tue, 16 Jun 2020 09:54:20: #2 number of paired peaks: 54 WARNING @ Tue, 16 Jun 2020 09:54:20: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:54:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:54:25: 19000000 INFO @ Tue, 16 Jun 2020 09:54:31: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:54:31: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:54:31: #1 total tags in treatment: 19980347 INFO @ Tue, 16 Jun 2020 09:54:31: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:54:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:54:32: #1 tags after filtering in treatment: 19980347 INFO @ Tue, 16 Jun 2020 09:54:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:54:32: #1 finished! INFO @ Tue, 16 Jun 2020 09:54:32: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:54:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:54:33: #2 number of paired peaks: 54 WARNING @ Tue, 16 Jun 2020 09:54:33: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:54:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971793/SRX7971793.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。