Job ID = 6368966 SRX = SRX7971792 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:36:49 prefetch.2.10.7: 1) Downloading 'SRR11392678'... 2020-06-16T00:36:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:41:45 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:41:45 prefetch.2.10.7: 1) 'SRR11392678' was downloaded successfully Read 27891436 spots for SRR11392678/SRR11392678.sra Written 27891436 spots for SRR11392678/SRR11392678.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:56 27891436 reads; of these: 27891436 (100.00%) were unpaired; of these: 506260 (1.82%) aligned 0 times 24669255 (88.45%) aligned exactly 1 time 2715921 (9.74%) aligned >1 times 98.18% overall alignment rate Time searching: 00:08:56 Overall time: 00:08:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5043225 / 27385176 = 0.1842 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:02:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:02:31: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:02:31: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:02:38: 1000000 INFO @ Tue, 16 Jun 2020 10:02:44: 2000000 INFO @ Tue, 16 Jun 2020 10:02:51: 3000000 INFO @ Tue, 16 Jun 2020 10:02:57: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:03:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:03:01: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:03:01: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:03:04: 5000000 INFO @ Tue, 16 Jun 2020 10:03:08: 1000000 INFO @ Tue, 16 Jun 2020 10:03:10: 6000000 INFO @ Tue, 16 Jun 2020 10:03:14: 2000000 INFO @ Tue, 16 Jun 2020 10:03:17: 7000000 INFO @ Tue, 16 Jun 2020 10:03:20: 3000000 INFO @ Tue, 16 Jun 2020 10:03:24: 8000000 INFO @ Tue, 16 Jun 2020 10:03:26: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:03:31: 9000000 INFO @ Tue, 16 Jun 2020 10:03:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:03:31: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:03:31: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:03:32: 5000000 INFO @ Tue, 16 Jun 2020 10:03:38: 1000000 INFO @ Tue, 16 Jun 2020 10:03:38: 10000000 INFO @ Tue, 16 Jun 2020 10:03:39: 6000000 INFO @ Tue, 16 Jun 2020 10:03:44: 2000000 INFO @ Tue, 16 Jun 2020 10:03:45: 11000000 INFO @ Tue, 16 Jun 2020 10:03:45: 7000000 INFO @ Tue, 16 Jun 2020 10:03:50: 3000000 INFO @ Tue, 16 Jun 2020 10:03:51: 8000000 INFO @ Tue, 16 Jun 2020 10:03:52: 12000000 INFO @ Tue, 16 Jun 2020 10:03:57: 4000000 INFO @ Tue, 16 Jun 2020 10:03:58: 9000000 INFO @ Tue, 16 Jun 2020 10:03:59: 13000000 INFO @ Tue, 16 Jun 2020 10:04:03: 5000000 INFO @ Tue, 16 Jun 2020 10:04:04: 10000000 INFO @ Tue, 16 Jun 2020 10:04:05: 14000000 INFO @ Tue, 16 Jun 2020 10:04:09: 6000000 INFO @ Tue, 16 Jun 2020 10:04:11: 11000000 INFO @ Tue, 16 Jun 2020 10:04:12: 15000000 INFO @ Tue, 16 Jun 2020 10:04:15: 7000000 INFO @ Tue, 16 Jun 2020 10:04:17: 12000000 INFO @ Tue, 16 Jun 2020 10:04:19: 16000000 INFO @ Tue, 16 Jun 2020 10:04:22: 8000000 INFO @ Tue, 16 Jun 2020 10:04:24: 13000000 INFO @ Tue, 16 Jun 2020 10:04:26: 17000000 INFO @ Tue, 16 Jun 2020 10:04:28: 9000000 INFO @ Tue, 16 Jun 2020 10:04:30: 14000000 INFO @ Tue, 16 Jun 2020 10:04:33: 18000000 INFO @ Tue, 16 Jun 2020 10:04:35: 10000000 INFO @ Tue, 16 Jun 2020 10:04:37: 15000000 INFO @ Tue, 16 Jun 2020 10:04:39: 19000000 INFO @ Tue, 16 Jun 2020 10:04:41: 11000000 INFO @ Tue, 16 Jun 2020 10:04:43: 16000000 INFO @ Tue, 16 Jun 2020 10:04:46: 20000000 INFO @ Tue, 16 Jun 2020 10:04:48: 12000000 INFO @ Tue, 16 Jun 2020 10:04:49: 17000000 INFO @ Tue, 16 Jun 2020 10:04:53: 21000000 INFO @ Tue, 16 Jun 2020 10:04:54: 13000000 INFO @ Tue, 16 Jun 2020 10:04:56: 18000000 INFO @ Tue, 16 Jun 2020 10:05:00: 22000000 INFO @ Tue, 16 Jun 2020 10:05:01: 14000000 INFO @ Tue, 16 Jun 2020 10:05:02: 19000000 INFO @ Tue, 16 Jun 2020 10:05:03: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:05:03: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:05:03: #1 total tags in treatment: 22341951 INFO @ Tue, 16 Jun 2020 10:05:03: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:05:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:05:03: #1 tags after filtering in treatment: 22341951 INFO @ Tue, 16 Jun 2020 10:05:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:05:03: #1 finished! INFO @ Tue, 16 Jun 2020 10:05:03: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:05:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:05:05: #2 number of paired peaks: 53 WARNING @ Tue, 16 Jun 2020 10:05:05: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:05:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:05:07: 15000000 INFO @ Tue, 16 Jun 2020 10:05:09: 20000000 INFO @ Tue, 16 Jun 2020 10:05:13: 16000000 INFO @ Tue, 16 Jun 2020 10:05:15: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:05:20: 17000000 INFO @ Tue, 16 Jun 2020 10:05:21: 22000000 INFO @ Tue, 16 Jun 2020 10:05:24: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:05:24: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:05:24: #1 total tags in treatment: 22341951 INFO @ Tue, 16 Jun 2020 10:05:24: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:05:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:05:24: #1 tags after filtering in treatment: 22341951 INFO @ Tue, 16 Jun 2020 10:05:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:05:24: #1 finished! INFO @ Tue, 16 Jun 2020 10:05:24: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:05:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:05:26: #2 number of paired peaks: 53 WARNING @ Tue, 16 Jun 2020 10:05:26: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:05:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:05:26: 18000000 INFO @ Tue, 16 Jun 2020 10:05:32: 19000000 INFO @ Tue, 16 Jun 2020 10:05:38: 20000000 INFO @ Tue, 16 Jun 2020 10:05:45: 21000000 INFO @ Tue, 16 Jun 2020 10:05:51: 22000000 INFO @ Tue, 16 Jun 2020 10:05:53: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:05:53: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:05:53: #1 total tags in treatment: 22341951 INFO @ Tue, 16 Jun 2020 10:05:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:05:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:05:53: #1 tags after filtering in treatment: 22341951 INFO @ Tue, 16 Jun 2020 10:05:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:05:53: #1 finished! INFO @ Tue, 16 Jun 2020 10:05:53: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:05:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:05:55: #2 number of paired peaks: 53 WARNING @ Tue, 16 Jun 2020 10:05:55: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:05:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971792/SRX7971792.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。