Job ID = 10165937 SRX = SRX7879258 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:20 39732741 reads; of these: 39732741 (100.00%) were unpaired; of these: 645300 (1.62%) aligned 0 times 33453353 (84.20%) aligned exactly 1 time 5634088 (14.18%) aligned >1 times 98.38% overall alignment rate Time searching: 00:09:20 Overall time: 00:09:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6881373 / 39087441 = 0.1761 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:29:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:29:52: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:29:52: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:29:58: 1000000 INFO @ Thu, 08 Oct 2020 20:30:03: 2000000 INFO @ Thu, 08 Oct 2020 20:30:09: 3000000 INFO @ Thu, 08 Oct 2020 20:30:14: 4000000 INFO @ Thu, 08 Oct 2020 20:30:20: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:30:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:30:22: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:30:22: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:30:26: 6000000 INFO @ Thu, 08 Oct 2020 20:30:29: 1000000 INFO @ Thu, 08 Oct 2020 20:30:33: 7000000 INFO @ Thu, 08 Oct 2020 20:30:35: 2000000 INFO @ Thu, 08 Oct 2020 20:30:39: 8000000 INFO @ Thu, 08 Oct 2020 20:30:42: 3000000 INFO @ Thu, 08 Oct 2020 20:30:46: 9000000 INFO @ Thu, 08 Oct 2020 20:30:48: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:30:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:30:52: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:30:52: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:30:52: 10000000 INFO @ Thu, 08 Oct 2020 20:30:55: 5000000 INFO @ Thu, 08 Oct 2020 20:30:59: 1000000 INFO @ Thu, 08 Oct 2020 20:30:59: 11000000 INFO @ Thu, 08 Oct 2020 20:31:02: 6000000 INFO @ Thu, 08 Oct 2020 20:31:06: 12000000 INFO @ Thu, 08 Oct 2020 20:31:06: 2000000 INFO @ Thu, 08 Oct 2020 20:31:09: 7000000 INFO @ Thu, 08 Oct 2020 20:31:12: 13000000 INFO @ Thu, 08 Oct 2020 20:31:12: 3000000 INFO @ Thu, 08 Oct 2020 20:31:15: 8000000 INFO @ Thu, 08 Oct 2020 20:31:19: 14000000 INFO @ Thu, 08 Oct 2020 20:31:19: 4000000 INFO @ Thu, 08 Oct 2020 20:31:22: 9000000 INFO @ Thu, 08 Oct 2020 20:31:25: 15000000 INFO @ Thu, 08 Oct 2020 20:31:26: 5000000 INFO @ Thu, 08 Oct 2020 20:31:29: 10000000 INFO @ Thu, 08 Oct 2020 20:31:32: 16000000 INFO @ Thu, 08 Oct 2020 20:31:33: 6000000 INFO @ Thu, 08 Oct 2020 20:31:36: 11000000 INFO @ Thu, 08 Oct 2020 20:31:38: 17000000 INFO @ Thu, 08 Oct 2020 20:31:40: 7000000 INFO @ Thu, 08 Oct 2020 20:31:43: 12000000 INFO @ Thu, 08 Oct 2020 20:31:45: 18000000 INFO @ Thu, 08 Oct 2020 20:31:46: 8000000 INFO @ Thu, 08 Oct 2020 20:31:49: 13000000 INFO @ Thu, 08 Oct 2020 20:31:51: 19000000 INFO @ Thu, 08 Oct 2020 20:31:53: 9000000 INFO @ Thu, 08 Oct 2020 20:31:56: 14000000 INFO @ Thu, 08 Oct 2020 20:31:58: 20000000 INFO @ Thu, 08 Oct 2020 20:32:00: 10000000 INFO @ Thu, 08 Oct 2020 20:32:03: 15000000 INFO @ Thu, 08 Oct 2020 20:32:04: 21000000 INFO @ Thu, 08 Oct 2020 20:32:07: 11000000 INFO @ Thu, 08 Oct 2020 20:32:10: 16000000 INFO @ Thu, 08 Oct 2020 20:32:11: 22000000 INFO @ Thu, 08 Oct 2020 20:32:14: 12000000 INFO @ Thu, 08 Oct 2020 20:32:17: 17000000 INFO @ Thu, 08 Oct 2020 20:32:17: 23000000 INFO @ Thu, 08 Oct 2020 20:32:20: 13000000 INFO @ Thu, 08 Oct 2020 20:32:24: 18000000 INFO @ Thu, 08 Oct 2020 20:32:24: 24000000 INFO @ Thu, 08 Oct 2020 20:32:27: 14000000 INFO @ Thu, 08 Oct 2020 20:32:30: 19000000 INFO @ Thu, 08 Oct 2020 20:32:30: 25000000 INFO @ Thu, 08 Oct 2020 20:32:34: 15000000 INFO @ Thu, 08 Oct 2020 20:32:37: 20000000 INFO @ Thu, 08 Oct 2020 20:32:37: 26000000 INFO @ Thu, 08 Oct 2020 20:32:40: 16000000 INFO @ Thu, 08 Oct 2020 20:32:43: 21000000 INFO @ Thu, 08 Oct 2020 20:32:43: 27000000 INFO @ Thu, 08 Oct 2020 20:32:47: 17000000 INFO @ Thu, 08 Oct 2020 20:32:50: 28000000 INFO @ Thu, 08 Oct 2020 20:32:50: 22000000 INFO @ Thu, 08 Oct 2020 20:32:54: 18000000 INFO @ Thu, 08 Oct 2020 20:32:57: 29000000 INFO @ Thu, 08 Oct 2020 20:32:57: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:33:00: 19000000 INFO @ Thu, 08 Oct 2020 20:33:03: 30000000 INFO @ Thu, 08 Oct 2020 20:33:03: 24000000 INFO @ Thu, 08 Oct 2020 20:33:07: 20000000 INFO @ Thu, 08 Oct 2020 20:33:10: 31000000 INFO @ Thu, 08 Oct 2020 20:33:10: 25000000 INFO @ Thu, 08 Oct 2020 20:33:14: 21000000 INFO @ Thu, 08 Oct 2020 20:33:16: 32000000 INFO @ Thu, 08 Oct 2020 20:33:17: 26000000 INFO @ Thu, 08 Oct 2020 20:33:18: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:33:18: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:33:18: #1 total tags in treatment: 32206068 INFO @ Thu, 08 Oct 2020 20:33:18: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:33:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:33:18: #1 tags after filtering in treatment: 32206068 INFO @ Thu, 08 Oct 2020 20:33:18: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:33:18: #1 finished! INFO @ Thu, 08 Oct 2020 20:33:18: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:33:18: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:33:20: #2 number of paired peaks: 23 WARNING @ Thu, 08 Oct 2020 20:33:20: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:33:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:33:20: 22000000 INFO @ Thu, 08 Oct 2020 20:33:23: 27000000 INFO @ Thu, 08 Oct 2020 20:33:27: 23000000 INFO @ Thu, 08 Oct 2020 20:33:30: 28000000 INFO @ Thu, 08 Oct 2020 20:33:34: 24000000 INFO @ Thu, 08 Oct 2020 20:33:37: 29000000 INFO @ Thu, 08 Oct 2020 20:33:40: 25000000 INFO @ Thu, 08 Oct 2020 20:33:43: 30000000 INFO @ Thu, 08 Oct 2020 20:33:47: 26000000 INFO @ Thu, 08 Oct 2020 20:33:50: 31000000 INFO @ Thu, 08 Oct 2020 20:33:53: 27000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 20:33:56: 32000000 INFO @ Thu, 08 Oct 2020 20:33:58: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:33:58: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:33:58: #1 total tags in treatment: 32206068 INFO @ Thu, 08 Oct 2020 20:33:58: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:33:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:33:58: #1 tags after filtering in treatment: 32206068 INFO @ Thu, 08 Oct 2020 20:33:58: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:33:58: #1 finished! INFO @ Thu, 08 Oct 2020 20:33:58: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:33:58: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:33:59: 28000000 INFO @ Thu, 08 Oct 2020 20:34:00: #2 number of paired peaks: 23 WARNING @ Thu, 08 Oct 2020 20:34:00: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:34:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:34:06: 29000000 INFO @ Thu, 08 Oct 2020 20:34:12: 30000000 INFO @ Thu, 08 Oct 2020 20:34:18: 31000000 INFO @ Thu, 08 Oct 2020 20:34:23: 32000000 INFO @ Thu, 08 Oct 2020 20:34:25: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:34:25: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:34:25: #1 total tags in treatment: 32206068 INFO @ Thu, 08 Oct 2020 20:34:25: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:34:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:34:25: #1 tags after filtering in treatment: 32206068 INFO @ Thu, 08 Oct 2020 20:34:25: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:34:25: #1 finished! INFO @ Thu, 08 Oct 2020 20:34:25: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:34:25: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:34:27: #2 number of paired peaks: 23 WARNING @ Thu, 08 Oct 2020 20:34:27: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:34:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling