Job ID = 14157992 SRX = SRX7879258 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:13:34 39732741 reads; of these: 39732741 (100.00%) were unpaired; of these: 645300 (1.62%) aligned 0 times 33453353 (84.20%) aligned exactly 1 time 5634088 (14.18%) aligned >1 times 98.38% overall alignment rate Time searching: 00:13:36 Overall time: 00:13:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6881373 / 39087441 = 0.1761 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:41:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:41:35: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:41:35: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:41:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:41:35: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:41:35: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:41:42: 1000000 INFO @ Wed, 08 Dec 2021 14:41:42: 1000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:41:50: 2000000 INFO @ Wed, 08 Dec 2021 14:41:50: 2000000 INFO @ Wed, 08 Dec 2021 14:41:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:41:50: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:41:50: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:41:58: 3000000 INFO @ Wed, 08 Dec 2021 14:41:58: 1000000 INFO @ Wed, 08 Dec 2021 14:41:58: 3000000 INFO @ Wed, 08 Dec 2021 14:42:05: 2000000 INFO @ Wed, 08 Dec 2021 14:42:05: 4000000 INFO @ Wed, 08 Dec 2021 14:42:06: 4000000 INFO @ Wed, 08 Dec 2021 14:42:12: 3000000 INFO @ Wed, 08 Dec 2021 14:42:12: 5000000 INFO @ Wed, 08 Dec 2021 14:42:13: 5000000 INFO @ Wed, 08 Dec 2021 14:42:18: 4000000 INFO @ Wed, 08 Dec 2021 14:42:19: 6000000 INFO @ Wed, 08 Dec 2021 14:42:20: 6000000 INFO @ Wed, 08 Dec 2021 14:42:25: 5000000 INFO @ Wed, 08 Dec 2021 14:42:25: 7000000 INFO @ Wed, 08 Dec 2021 14:42:27: 7000000 INFO @ Wed, 08 Dec 2021 14:42:32: 6000000 INFO @ Wed, 08 Dec 2021 14:42:32: 8000000 INFO @ Wed, 08 Dec 2021 14:42:34: 8000000 INFO @ Wed, 08 Dec 2021 14:42:38: 7000000 INFO @ Wed, 08 Dec 2021 14:42:39: 9000000 INFO @ Wed, 08 Dec 2021 14:42:40: 9000000 INFO @ Wed, 08 Dec 2021 14:42:45: 8000000 INFO @ Wed, 08 Dec 2021 14:42:45: 10000000 INFO @ Wed, 08 Dec 2021 14:42:47: 10000000 INFO @ Wed, 08 Dec 2021 14:42:52: 9000000 INFO @ Wed, 08 Dec 2021 14:42:52: 11000000 INFO @ Wed, 08 Dec 2021 14:42:54: 11000000 INFO @ Wed, 08 Dec 2021 14:42:58: 10000000 INFO @ Wed, 08 Dec 2021 14:42:58: 12000000 INFO @ Wed, 08 Dec 2021 14:43:00: 12000000 INFO @ Wed, 08 Dec 2021 14:43:05: 13000000 INFO @ Wed, 08 Dec 2021 14:43:05: 11000000 INFO @ Wed, 08 Dec 2021 14:43:07: 13000000 INFO @ Wed, 08 Dec 2021 14:43:11: 14000000 INFO @ Wed, 08 Dec 2021 14:43:11: 12000000 INFO @ Wed, 08 Dec 2021 14:43:13: 14000000 INFO @ Wed, 08 Dec 2021 14:43:17: 15000000 INFO @ Wed, 08 Dec 2021 14:43:18: 13000000 INFO @ Wed, 08 Dec 2021 14:43:19: 15000000 INFO @ Wed, 08 Dec 2021 14:43:24: 16000000 INFO @ Wed, 08 Dec 2021 14:43:24: 14000000 INFO @ Wed, 08 Dec 2021 14:43:26: 16000000 INFO @ Wed, 08 Dec 2021 14:43:30: 17000000 INFO @ Wed, 08 Dec 2021 14:43:31: 15000000 INFO @ Wed, 08 Dec 2021 14:43:32: 17000000 INFO @ Wed, 08 Dec 2021 14:43:37: 18000000 INFO @ Wed, 08 Dec 2021 14:43:37: 16000000 INFO @ Wed, 08 Dec 2021 14:43:39: 18000000 INFO @ Wed, 08 Dec 2021 14:43:43: 19000000 INFO @ Wed, 08 Dec 2021 14:43:44: 17000000 INFO @ Wed, 08 Dec 2021 14:43:45: 19000000 INFO @ Wed, 08 Dec 2021 14:43:50: 20000000 INFO @ Wed, 08 Dec 2021 14:43:50: 18000000 INFO @ Wed, 08 Dec 2021 14:43:52: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 14:43:56: 21000000 INFO @ Wed, 08 Dec 2021 14:43:56: 19000000 INFO @ Wed, 08 Dec 2021 14:43:58: 21000000 INFO @ Wed, 08 Dec 2021 14:44:02: 22000000 INFO @ Wed, 08 Dec 2021 14:44:03: 20000000 INFO @ Wed, 08 Dec 2021 14:44:04: 22000000 INFO @ Wed, 08 Dec 2021 14:44:09: 23000000 INFO @ Wed, 08 Dec 2021 14:44:09: 21000000 INFO @ Wed, 08 Dec 2021 14:44:11: 23000000 INFO @ Wed, 08 Dec 2021 14:44:15: 24000000 INFO @ Wed, 08 Dec 2021 14:44:16: 22000000 INFO @ Wed, 08 Dec 2021 14:44:17: 24000000 INFO @ Wed, 08 Dec 2021 14:44:21: 25000000 INFO @ Wed, 08 Dec 2021 14:44:22: 23000000 INFO @ Wed, 08 Dec 2021 14:44:24: 25000000 INFO @ Wed, 08 Dec 2021 14:44:28: 26000000 INFO @ Wed, 08 Dec 2021 14:44:29: 24000000 INFO @ Wed, 08 Dec 2021 14:44:30: 26000000 INFO @ Wed, 08 Dec 2021 14:44:34: 27000000 INFO @ Wed, 08 Dec 2021 14:44:35: 25000000 INFO @ Wed, 08 Dec 2021 14:44:36: 27000000 INFO @ Wed, 08 Dec 2021 14:44:41: 28000000 INFO @ Wed, 08 Dec 2021 14:44:41: 26000000 INFO @ Wed, 08 Dec 2021 14:44:43: 28000000 INFO @ Wed, 08 Dec 2021 14:44:47: 29000000 INFO @ Wed, 08 Dec 2021 14:44:48: 27000000 INFO @ Wed, 08 Dec 2021 14:44:49: 29000000 INFO @ Wed, 08 Dec 2021 14:44:53: 30000000 INFO @ Wed, 08 Dec 2021 14:44:54: 28000000 INFO @ Wed, 08 Dec 2021 14:44:55: 30000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 14:45:00: 31000000 INFO @ Wed, 08 Dec 2021 14:45:01: 29000000 INFO @ Wed, 08 Dec 2021 14:45:02: 31000000 INFO @ Wed, 08 Dec 2021 14:45:06: 32000000 INFO @ Wed, 08 Dec 2021 14:45:07: 30000000 INFO @ Wed, 08 Dec 2021 14:45:08: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 14:45:08: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 14:45:08: #1 total tags in treatment: 32206068 INFO @ Wed, 08 Dec 2021 14:45:08: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:45:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:45:08: #1 tags after filtering in treatment: 32206068 INFO @ Wed, 08 Dec 2021 14:45:08: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:45:08: #1 finished! INFO @ Wed, 08 Dec 2021 14:45:08: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:45:08: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:45:08: 32000000 INFO @ Wed, 08 Dec 2021 14:45:10: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 14:45:10: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 14:45:10: #1 total tags in treatment: 32206068 INFO @ Wed, 08 Dec 2021 14:45:10: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:45:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:45:10: #2 number of paired peaks: 23 WARNING @ Wed, 08 Dec 2021 14:45:10: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:45:10: Process for pairing-model is terminated! INFO @ Wed, 08 Dec 2021 14:45:10: #1 tags after filtering in treatment: 32206068 INFO @ Wed, 08 Dec 2021 14:45:10: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:45:10: #1 finished! INFO @ Wed, 08 Dec 2021 14:45:10: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:45:10: #2 looking for paired plus/minus strand peaks... cut: /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 14:45:12: #2 number of paired peaks: 23 WARNING @ Wed, 08 Dec 2021 14:45:12: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:45:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 14:45:13: 31000000 INFO @ Wed, 08 Dec 2021 14:45:19: 32000000 INFO @ Wed, 08 Dec 2021 14:45:21: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 14:45:21: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 14:45:21: #1 total tags in treatment: 32206068 INFO @ Wed, 08 Dec 2021 14:45:21: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:45:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:45:21: #1 tags after filtering in treatment: 32206068 INFO @ Wed, 08 Dec 2021 14:45:21: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:45:21: #1 finished! INFO @ Wed, 08 Dec 2021 14:45:21: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:45:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:45:23: #2 number of paired peaks: 23 WARNING @ Wed, 08 Dec 2021 14:45:23: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:45:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879258/SRX7879258.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling