Job ID = 14157993 SRX = SRX7879257 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:44 44815642 reads; of these: 44815642 (100.00%) were unpaired; of these: 453042 (1.01%) aligned 0 times 37671277 (84.06%) aligned exactly 1 time 6691323 (14.93%) aligned >1 times 98.99% overall alignment rate Time searching: 00:09:44 Overall time: 00:09:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8399745 / 44362600 = 0.1893 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:03:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:03:45: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:03:45: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:03:50: 1000000 INFO @ Wed, 08 Dec 2021 14:03:55: 2000000 INFO @ Wed, 08 Dec 2021 14:04:00: 3000000 INFO @ Wed, 08 Dec 2021 14:04:05: 4000000 INFO @ Wed, 08 Dec 2021 14:04:10: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:04:15: 6000000 INFO @ Wed, 08 Dec 2021 14:04:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:04:15: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:04:15: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:04:20: 7000000 INFO @ Wed, 08 Dec 2021 14:04:21: 1000000 INFO @ Wed, 08 Dec 2021 14:04:26: 8000000 INFO @ Wed, 08 Dec 2021 14:04:27: 2000000 INFO @ Wed, 08 Dec 2021 14:04:31: 9000000 INFO @ Wed, 08 Dec 2021 14:04:32: 3000000 INFO @ Wed, 08 Dec 2021 14:04:37: 10000000 INFO @ Wed, 08 Dec 2021 14:04:38: 4000000 INFO @ Wed, 08 Dec 2021 14:04:42: 11000000 INFO @ Wed, 08 Dec 2021 14:04:43: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:04:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:04:45: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:04:45: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:04:48: 12000000 INFO @ Wed, 08 Dec 2021 14:04:49: 6000000 INFO @ Wed, 08 Dec 2021 14:04:52: 1000000 INFO @ Wed, 08 Dec 2021 14:04:53: 13000000 INFO @ Wed, 08 Dec 2021 14:04:54: 7000000 INFO @ Wed, 08 Dec 2021 14:04:58: 2000000 INFO @ Wed, 08 Dec 2021 14:04:59: 14000000 INFO @ Wed, 08 Dec 2021 14:05:00: 8000000 INFO @ Wed, 08 Dec 2021 14:05:05: 3000000 INFO @ Wed, 08 Dec 2021 14:05:05: 15000000 INFO @ Wed, 08 Dec 2021 14:05:06: 9000000 INFO @ Wed, 08 Dec 2021 14:05:10: 16000000 INFO @ Wed, 08 Dec 2021 14:05:11: 4000000 INFO @ Wed, 08 Dec 2021 14:05:12: 10000000 INFO @ Wed, 08 Dec 2021 14:05:16: 17000000 INFO @ Wed, 08 Dec 2021 14:05:17: 11000000 INFO @ Wed, 08 Dec 2021 14:05:17: 5000000 INFO @ Wed, 08 Dec 2021 14:05:22: 18000000 INFO @ Wed, 08 Dec 2021 14:05:23: 12000000 INFO @ Wed, 08 Dec 2021 14:05:24: 6000000 INFO @ Wed, 08 Dec 2021 14:05:27: 19000000 INFO @ Wed, 08 Dec 2021 14:05:29: 13000000 INFO @ Wed, 08 Dec 2021 14:05:30: 7000000 INFO @ Wed, 08 Dec 2021 14:05:33: 20000000 INFO @ Wed, 08 Dec 2021 14:05:34: 14000000 INFO @ Wed, 08 Dec 2021 14:05:37: 8000000 INFO @ Wed, 08 Dec 2021 14:05:39: 21000000 INFO @ Wed, 08 Dec 2021 14:05:40: 15000000 INFO @ Wed, 08 Dec 2021 14:05:43: 9000000 INFO @ Wed, 08 Dec 2021 14:05:44: 22000000 INFO @ Wed, 08 Dec 2021 14:05:46: 16000000 INFO @ Wed, 08 Dec 2021 14:05:49: 10000000 INFO @ Wed, 08 Dec 2021 14:05:50: 23000000 INFO @ Wed, 08 Dec 2021 14:05:51: 17000000 INFO @ Wed, 08 Dec 2021 14:05:56: 11000000 INFO @ Wed, 08 Dec 2021 14:05:56: 24000000 INFO @ Wed, 08 Dec 2021 14:05:57: 18000000 INFO @ Wed, 08 Dec 2021 14:06:02: 25000000 INFO @ Wed, 08 Dec 2021 14:06:02: 12000000 INFO @ Wed, 08 Dec 2021 14:06:03: 19000000 INFO @ Wed, 08 Dec 2021 14:06:07: 26000000 INFO @ Wed, 08 Dec 2021 14:06:08: 13000000 INFO @ Wed, 08 Dec 2021 14:06:09: 20000000 INFO @ Wed, 08 Dec 2021 14:06:13: 27000000 INFO @ Wed, 08 Dec 2021 14:06:14: 21000000 INFO @ Wed, 08 Dec 2021 14:06:15: 14000000 INFO @ Wed, 08 Dec 2021 14:06:19: 28000000 INFO @ Wed, 08 Dec 2021 14:06:20: 22000000 INFO @ Wed, 08 Dec 2021 14:06:21: 15000000 INFO @ Wed, 08 Dec 2021 14:06:24: 29000000 INFO @ Wed, 08 Dec 2021 14:06:26: 23000000 INFO @ Wed, 08 Dec 2021 14:06:28: 16000000 INFO @ Wed, 08 Dec 2021 14:06:30: 30000000 INFO @ Wed, 08 Dec 2021 14:06:31: 24000000 INFO @ Wed, 08 Dec 2021 14:06:34: 17000000 INFO @ Wed, 08 Dec 2021 14:06:36: 31000000 INFO @ Wed, 08 Dec 2021 14:06:37: 25000000 INFO @ Wed, 08 Dec 2021 14:06:41: 18000000 INFO @ Wed, 08 Dec 2021 14:06:41: 32000000 INFO @ Wed, 08 Dec 2021 14:06:43: 26000000 INFO @ Wed, 08 Dec 2021 14:06:47: 33000000 INFO @ Wed, 08 Dec 2021 14:06:47: 19000000 INFO @ Wed, 08 Dec 2021 14:06:48: 27000000 INFO @ Wed, 08 Dec 2021 14:06:53: 34000000 INFO @ Wed, 08 Dec 2021 14:06:54: 20000000 INFO @ Wed, 08 Dec 2021 14:06:54: 28000000 INFO @ Wed, 08 Dec 2021 14:06:59: 35000000 INFO @ Wed, 08 Dec 2021 14:07:00: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 14:07:00: 21000000 INFO @ Wed, 08 Dec 2021 14:07:04: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 14:07:04: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 14:07:04: #1 total tags in treatment: 35962855 INFO @ Wed, 08 Dec 2021 14:07:04: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:07:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:07:05: #1 tags after filtering in treatment: 35962855 INFO @ Wed, 08 Dec 2021 14:07:05: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:07:05: #1 finished! INFO @ Wed, 08 Dec 2021 14:07:05: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:07:05: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:07:05: 30000000 INFO @ Wed, 08 Dec 2021 14:07:07: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 14:07:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:07:07: Process for pairing-model is terminated! INFO @ Wed, 08 Dec 2021 14:07:07: 22000000 cut: /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 14:07:11: 31000000 INFO @ Wed, 08 Dec 2021 14:07:13: 23000000 INFO @ Wed, 08 Dec 2021 14:07:17: 32000000 INFO @ Wed, 08 Dec 2021 14:07:20: 24000000 INFO @ Wed, 08 Dec 2021 14:07:22: 33000000 INFO @ Wed, 08 Dec 2021 14:07:26: 25000000 INFO @ Wed, 08 Dec 2021 14:07:28: 34000000 INFO @ Wed, 08 Dec 2021 14:07:33: 26000000 INFO @ Wed, 08 Dec 2021 14:07:34: 35000000 INFO @ Wed, 08 Dec 2021 14:07:39: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 14:07:39: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 14:07:39: #1 total tags in treatment: 35962855 INFO @ Wed, 08 Dec 2021 14:07:39: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:07:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:07:40: 27000000 INFO @ Wed, 08 Dec 2021 14:07:40: #1 tags after filtering in treatment: 35962855 INFO @ Wed, 08 Dec 2021 14:07:40: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:07:40: #1 finished! INFO @ Wed, 08 Dec 2021 14:07:40: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:07:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:07:42: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 14:07:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:07:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 14:07:46: 28000000 INFO @ Wed, 08 Dec 2021 14:07:52: 29000000 INFO @ Wed, 08 Dec 2021 14:07:58: 30000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 14:08:04: 31000000 INFO @ Wed, 08 Dec 2021 14:08:10: 32000000 INFO @ Wed, 08 Dec 2021 14:08:17: 33000000 INFO @ Wed, 08 Dec 2021 14:08:23: 34000000 INFO @ Wed, 08 Dec 2021 14:08:29: 35000000 INFO @ Wed, 08 Dec 2021 14:08:35: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 14:08:35: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 14:08:35: #1 total tags in treatment: 35962855 INFO @ Wed, 08 Dec 2021 14:08:35: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:08:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:08:35: #1 tags after filtering in treatment: 35962855 INFO @ Wed, 08 Dec 2021 14:08:35: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:08:35: #1 finished! INFO @ Wed, 08 Dec 2021 14:08:35: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:08:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:08:38: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 14:08:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:08:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling