Job ID = 10165951 SRX = SRX7879257 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:10:06 44815642 reads; of these: 44815642 (100.00%) were unpaired; of these: 453042 (1.01%) aligned 0 times 37671277 (84.06%) aligned exactly 1 time 6691323 (14.93%) aligned >1 times 98.99% overall alignment rate Time searching: 00:10:07 Overall time: 00:10:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8399745 / 44362600 = 0.1893 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:34:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:34:39: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:34:39: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:34:44: 1000000 INFO @ Thu, 08 Oct 2020 20:34:49: 2000000 INFO @ Thu, 08 Oct 2020 20:34:53: 3000000 INFO @ Thu, 08 Oct 2020 20:34:58: 4000000 INFO @ Thu, 08 Oct 2020 20:35:03: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:35:08: 6000000 INFO @ Thu, 08 Oct 2020 20:35:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:35:09: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:35:09: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:35:13: 7000000 INFO @ Thu, 08 Oct 2020 20:35:14: 1000000 INFO @ Thu, 08 Oct 2020 20:35:19: 8000000 INFO @ Thu, 08 Oct 2020 20:35:19: 2000000 INFO @ Thu, 08 Oct 2020 20:35:24: 9000000 INFO @ Thu, 08 Oct 2020 20:35:25: 3000000 INFO @ Thu, 08 Oct 2020 20:35:29: 10000000 INFO @ Thu, 08 Oct 2020 20:35:30: 4000000 INFO @ Thu, 08 Oct 2020 20:35:35: 11000000 INFO @ Thu, 08 Oct 2020 20:35:35: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:35:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:35:39: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:35:39: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:35:40: 12000000 INFO @ Thu, 08 Oct 2020 20:35:41: 6000000 INFO @ Thu, 08 Oct 2020 20:35:45: 1000000 INFO @ Thu, 08 Oct 2020 20:35:46: 13000000 INFO @ Thu, 08 Oct 2020 20:35:47: 7000000 INFO @ Thu, 08 Oct 2020 20:35:51: 14000000 INFO @ Thu, 08 Oct 2020 20:35:51: 2000000 INFO @ Thu, 08 Oct 2020 20:35:52: 8000000 INFO @ Thu, 08 Oct 2020 20:35:57: 15000000 INFO @ Thu, 08 Oct 2020 20:35:58: 9000000 INFO @ Thu, 08 Oct 2020 20:35:58: 3000000 INFO @ Thu, 08 Oct 2020 20:36:02: 16000000 INFO @ Thu, 08 Oct 2020 20:36:03: 10000000 INFO @ Thu, 08 Oct 2020 20:36:04: 4000000 INFO @ Thu, 08 Oct 2020 20:36:08: 17000000 INFO @ Thu, 08 Oct 2020 20:36:09: 11000000 INFO @ Thu, 08 Oct 2020 20:36:11: 5000000 INFO @ Thu, 08 Oct 2020 20:36:13: 18000000 INFO @ Thu, 08 Oct 2020 20:36:14: 12000000 INFO @ Thu, 08 Oct 2020 20:36:17: 6000000 INFO @ Thu, 08 Oct 2020 20:36:19: 19000000 INFO @ Thu, 08 Oct 2020 20:36:20: 13000000 INFO @ Thu, 08 Oct 2020 20:36:24: 7000000 INFO @ Thu, 08 Oct 2020 20:36:25: 20000000 INFO @ Thu, 08 Oct 2020 20:36:26: 14000000 INFO @ Thu, 08 Oct 2020 20:36:30: 21000000 INFO @ Thu, 08 Oct 2020 20:36:30: 8000000 INFO @ Thu, 08 Oct 2020 20:36:31: 15000000 INFO @ Thu, 08 Oct 2020 20:36:36: 22000000 INFO @ Thu, 08 Oct 2020 20:36:37: 9000000 INFO @ Thu, 08 Oct 2020 20:36:37: 16000000 INFO @ Thu, 08 Oct 2020 20:36:41: 23000000 INFO @ Thu, 08 Oct 2020 20:36:42: 17000000 INFO @ Thu, 08 Oct 2020 20:36:43: 10000000 INFO @ Thu, 08 Oct 2020 20:36:47: 24000000 INFO @ Thu, 08 Oct 2020 20:36:48: 18000000 INFO @ Thu, 08 Oct 2020 20:36:49: 11000000 INFO @ Thu, 08 Oct 2020 20:36:53: 25000000 INFO @ Thu, 08 Oct 2020 20:36:54: 19000000 INFO @ Thu, 08 Oct 2020 20:36:56: 12000000 INFO @ Thu, 08 Oct 2020 20:36:58: 26000000 INFO @ Thu, 08 Oct 2020 20:36:59: 20000000 INFO @ Thu, 08 Oct 2020 20:37:02: 13000000 INFO @ Thu, 08 Oct 2020 20:37:04: 27000000 INFO @ Thu, 08 Oct 2020 20:37:05: 21000000 INFO @ Thu, 08 Oct 2020 20:37:08: 14000000 INFO @ Thu, 08 Oct 2020 20:37:09: 28000000 INFO @ Thu, 08 Oct 2020 20:37:10: 22000000 INFO @ Thu, 08 Oct 2020 20:37:14: 15000000 INFO @ Thu, 08 Oct 2020 20:37:15: 29000000 INFO @ Thu, 08 Oct 2020 20:37:16: 23000000 INFO @ Thu, 08 Oct 2020 20:37:20: 16000000 INFO @ Thu, 08 Oct 2020 20:37:21: 30000000 INFO @ Thu, 08 Oct 2020 20:37:22: 24000000 INFO @ Thu, 08 Oct 2020 20:37:26: 17000000 INFO @ Thu, 08 Oct 2020 20:37:26: 31000000 INFO @ Thu, 08 Oct 2020 20:37:27: 25000000 INFO @ Thu, 08 Oct 2020 20:37:32: 18000000 INFO @ Thu, 08 Oct 2020 20:37:32: 32000000 INFO @ Thu, 08 Oct 2020 20:37:33: 26000000 INFO @ Thu, 08 Oct 2020 20:37:38: 33000000 INFO @ Thu, 08 Oct 2020 20:37:38: 19000000 INFO @ Thu, 08 Oct 2020 20:37:39: 27000000 INFO @ Thu, 08 Oct 2020 20:37:44: 34000000 INFO @ Thu, 08 Oct 2020 20:37:44: 20000000 INFO @ Thu, 08 Oct 2020 20:37:44: 28000000 INFO @ Thu, 08 Oct 2020 20:37:49: 35000000 INFO @ Thu, 08 Oct 2020 20:37:50: 29000000 INFO @ Thu, 08 Oct 2020 20:37:50: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:37:55: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:37:55: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:37:55: #1 total tags in treatment: 35962855 INFO @ Thu, 08 Oct 2020 20:37:55: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:37:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:37:55: #1 tags after filtering in treatment: 35962855 INFO @ Thu, 08 Oct 2020 20:37:55: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:37:55: #1 finished! INFO @ Thu, 08 Oct 2020 20:37:55: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:37:55: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:37:56: 30000000 INFO @ Thu, 08 Oct 2020 20:37:56: 22000000 INFO @ Thu, 08 Oct 2020 20:37:58: #2 number of paired peaks: 0 WARNING @ Thu, 08 Oct 2020 20:37:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:37:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:38:01: 31000000 INFO @ Thu, 08 Oct 2020 20:38:02: 23000000 INFO @ Thu, 08 Oct 2020 20:38:07: 32000000 INFO @ Thu, 08 Oct 2020 20:38:09: 24000000 INFO @ Thu, 08 Oct 2020 20:38:13: 33000000 INFO @ Thu, 08 Oct 2020 20:38:15: 25000000 INFO @ Thu, 08 Oct 2020 20:38:18: 34000000 INFO @ Thu, 08 Oct 2020 20:38:21: 26000000 INFO @ Thu, 08 Oct 2020 20:38:24: 35000000 INFO @ Thu, 08 Oct 2020 20:38:27: 27000000 INFO @ Thu, 08 Oct 2020 20:38:30: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:38:30: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:38:30: #1 total tags in treatment: 35962855 INFO @ Thu, 08 Oct 2020 20:38:30: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:38:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:38:30: #1 tags after filtering in treatment: 35962855 INFO @ Thu, 08 Oct 2020 20:38:30: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:38:30: #1 finished! INFO @ Thu, 08 Oct 2020 20:38:30: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:38:30: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:38:32: #2 number of paired peaks: 0 WARNING @ Thu, 08 Oct 2020 20:38:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:38:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:38:33: 28000000 INFO @ Thu, 08 Oct 2020 20:38:39: 29000000 INFO @ Thu, 08 Oct 2020 20:38:44: 30000000 INFO @ Thu, 08 Oct 2020 20:38:50: 31000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 20:38:56: 32000000 INFO @ Thu, 08 Oct 2020 20:39:02: 33000000 INFO @ Thu, 08 Oct 2020 20:39:08: 34000000 INFO @ Thu, 08 Oct 2020 20:39:14: 35000000 INFO @ Thu, 08 Oct 2020 20:39:20: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:39:20: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:39:20: #1 total tags in treatment: 35962855 INFO @ Thu, 08 Oct 2020 20:39:20: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:39:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:39:20: #1 tags after filtering in treatment: 35962855 INFO @ Thu, 08 Oct 2020 20:39:20: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:39:20: #1 finished! INFO @ Thu, 08 Oct 2020 20:39:20: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:39:20: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:39:23: #2 number of paired peaks: 0 WARNING @ Thu, 08 Oct 2020 20:39:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:39:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879257/SRX7879257.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling