Job ID = 14157982 SRX = SRX7879253 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:24 45228743 reads; of these: 45228743 (100.00%) were unpaired; of these: 541047 (1.20%) aligned 0 times 36334657 (80.34%) aligned exactly 1 time 8353039 (18.47%) aligned >1 times 98.80% overall alignment rate Time searching: 00:11:24 Overall time: 00:11:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8806188 / 44687696 = 0.1971 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 13:57:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 13:57:12: #1 read tag files... INFO @ Wed, 08 Dec 2021 13:57:12: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 13:57:21: 1000000 INFO @ Wed, 08 Dec 2021 13:57:29: 2000000 INFO @ Wed, 08 Dec 2021 13:57:38: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 13:57:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 13:57:42: #1 read tag files... INFO @ Wed, 08 Dec 2021 13:57:42: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 13:57:46: 4000000 INFO @ Wed, 08 Dec 2021 13:57:54: 1000000 INFO @ Wed, 08 Dec 2021 13:57:55: 5000000 INFO @ Wed, 08 Dec 2021 13:58:03: 6000000 INFO @ Wed, 08 Dec 2021 13:58:05: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 13:58:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 13:58:12: #1 read tag files... INFO @ Wed, 08 Dec 2021 13:58:12: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 13:58:12: 7000000 INFO @ Wed, 08 Dec 2021 13:58:17: 3000000 INFO @ Wed, 08 Dec 2021 13:58:20: 8000000 INFO @ Wed, 08 Dec 2021 13:58:23: 1000000 INFO @ Wed, 08 Dec 2021 13:58:28: 4000000 INFO @ Wed, 08 Dec 2021 13:58:29: 9000000 INFO @ Wed, 08 Dec 2021 13:58:34: 2000000 INFO @ Wed, 08 Dec 2021 13:58:38: 10000000 INFO @ Wed, 08 Dec 2021 13:58:39: 5000000 INFO @ Wed, 08 Dec 2021 13:58:44: 3000000 INFO @ Wed, 08 Dec 2021 13:58:47: 11000000 INFO @ Wed, 08 Dec 2021 13:58:50: 6000000 INFO @ Wed, 08 Dec 2021 13:58:55: 4000000 INFO @ Wed, 08 Dec 2021 13:58:55: 12000000 INFO @ Wed, 08 Dec 2021 13:59:02: 7000000 INFO @ Wed, 08 Dec 2021 13:59:04: 13000000 INFO @ Wed, 08 Dec 2021 13:59:05: 5000000 INFO @ Wed, 08 Dec 2021 13:59:12: 8000000 INFO @ Wed, 08 Dec 2021 13:59:13: 14000000 INFO @ Wed, 08 Dec 2021 13:59:16: 6000000 INFO @ Wed, 08 Dec 2021 13:59:21: 15000000 INFO @ Wed, 08 Dec 2021 13:59:22: 9000000 INFO @ Wed, 08 Dec 2021 13:59:26: 7000000 INFO @ Wed, 08 Dec 2021 13:59:30: 16000000 INFO @ Wed, 08 Dec 2021 13:59:33: 10000000 INFO @ Wed, 08 Dec 2021 13:59:36: 8000000 INFO @ Wed, 08 Dec 2021 13:59:39: 17000000 INFO @ Wed, 08 Dec 2021 13:59:43: 11000000 INFO @ Wed, 08 Dec 2021 13:59:47: 9000000 INFO @ Wed, 08 Dec 2021 13:59:47: 18000000 INFO @ Wed, 08 Dec 2021 13:59:53: 12000000 INFO @ Wed, 08 Dec 2021 13:59:56: 19000000 INFO @ Wed, 08 Dec 2021 13:59:57: 10000000 INFO @ Wed, 08 Dec 2021 14:00:04: 13000000 INFO @ Wed, 08 Dec 2021 14:00:04: 20000000 INFO @ Wed, 08 Dec 2021 14:00:08: 11000000 INFO @ Wed, 08 Dec 2021 14:00:13: 21000000 INFO @ Wed, 08 Dec 2021 14:00:15: 14000000 INFO @ Wed, 08 Dec 2021 14:00:18: 12000000 INFO @ Wed, 08 Dec 2021 14:00:22: 22000000 INFO @ Wed, 08 Dec 2021 14:00:26: 15000000 INFO @ Wed, 08 Dec 2021 14:00:29: 13000000 INFO @ Wed, 08 Dec 2021 14:00:31: 23000000 INFO @ Wed, 08 Dec 2021 14:00:37: 16000000 INFO @ Wed, 08 Dec 2021 14:00:39: 14000000 INFO @ Wed, 08 Dec 2021 14:00:39: 24000000 INFO @ Wed, 08 Dec 2021 14:00:48: 17000000 INFO @ Wed, 08 Dec 2021 14:00:48: 25000000 INFO @ Wed, 08 Dec 2021 14:00:50: 15000000 INFO @ Wed, 08 Dec 2021 14:00:57: 26000000 INFO @ Wed, 08 Dec 2021 14:00:58: 18000000 INFO @ Wed, 08 Dec 2021 14:01:00: 16000000 INFO @ Wed, 08 Dec 2021 14:01:06: 27000000 INFO @ Wed, 08 Dec 2021 14:01:09: 19000000 INFO @ Wed, 08 Dec 2021 14:01:10: 17000000 INFO @ Wed, 08 Dec 2021 14:01:14: 28000000 INFO @ Wed, 08 Dec 2021 14:01:20: 20000000 INFO @ Wed, 08 Dec 2021 14:01:20: 18000000 INFO @ Wed, 08 Dec 2021 14:01:24: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 14:01:31: 19000000 INFO @ Wed, 08 Dec 2021 14:01:31: 21000000 INFO @ Wed, 08 Dec 2021 14:01:33: 30000000 INFO @ Wed, 08 Dec 2021 14:01:41: 20000000 INFO @ Wed, 08 Dec 2021 14:01:41: 31000000 INFO @ Wed, 08 Dec 2021 14:01:43: 22000000 INFO @ Wed, 08 Dec 2021 14:01:50: 32000000 INFO @ Wed, 08 Dec 2021 14:01:51: 21000000 INFO @ Wed, 08 Dec 2021 14:01:53: 23000000 INFO @ Wed, 08 Dec 2021 14:01:59: 33000000 INFO @ Wed, 08 Dec 2021 14:02:02: 22000000 INFO @ Wed, 08 Dec 2021 14:02:04: 24000000 INFO @ Wed, 08 Dec 2021 14:02:08: 34000000 INFO @ Wed, 08 Dec 2021 14:02:12: 23000000 INFO @ Wed, 08 Dec 2021 14:02:15: 25000000 INFO @ Wed, 08 Dec 2021 14:02:17: 35000000 INFO @ Wed, 08 Dec 2021 14:02:22: 24000000 INFO @ Wed, 08 Dec 2021 14:02:25: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 14:02:25: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 14:02:25: #1 total tags in treatment: 35881508 INFO @ Wed, 08 Dec 2021 14:02:25: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:02:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:02:26: #1 tags after filtering in treatment: 35881508 INFO @ Wed, 08 Dec 2021 14:02:26: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:02:26: #1 finished! INFO @ Wed, 08 Dec 2021 14:02:26: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:02:26: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:02:26: 26000000 INFO @ Wed, 08 Dec 2021 14:02:28: #2 number of paired peaks: 7 WARNING @ Wed, 08 Dec 2021 14:02:28: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:02:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 14:02:32: 25000000 INFO @ Wed, 08 Dec 2021 14:02:37: 27000000 INFO @ Wed, 08 Dec 2021 14:02:42: 26000000 INFO @ Wed, 08 Dec 2021 14:02:48: 28000000 INFO @ Wed, 08 Dec 2021 14:02:53: 27000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 14:03:00: 29000000 INFO @ Wed, 08 Dec 2021 14:03:03: 28000000 INFO @ Wed, 08 Dec 2021 14:03:11: 30000000 INFO @ Wed, 08 Dec 2021 14:03:14: 29000000 INFO @ Wed, 08 Dec 2021 14:03:23: 31000000 INFO @ Wed, 08 Dec 2021 14:03:24: 30000000 INFO @ Wed, 08 Dec 2021 14:03:34: 32000000 INFO @ Wed, 08 Dec 2021 14:03:35: 31000000 INFO @ Wed, 08 Dec 2021 14:03:45: 32000000 INFO @ Wed, 08 Dec 2021 14:03:46: 33000000 INFO @ Wed, 08 Dec 2021 14:03:56: 33000000 INFO @ Wed, 08 Dec 2021 14:03:57: 34000000 INFO @ Wed, 08 Dec 2021 14:04:07: 34000000 INFO @ Wed, 08 Dec 2021 14:04:09: 35000000 INFO @ Wed, 08 Dec 2021 14:04:17: 35000000 INFO @ Wed, 08 Dec 2021 14:04:19: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 14:04:19: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 14:04:19: #1 total tags in treatment: 35881508 INFO @ Wed, 08 Dec 2021 14:04:19: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:04:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:04:20: #1 tags after filtering in treatment: 35881508 INFO @ Wed, 08 Dec 2021 14:04:20: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:04:20: #1 finished! INFO @ Wed, 08 Dec 2021 14:04:20: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:04:20: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:04:22: #2 number of paired peaks: 7 WARNING @ Wed, 08 Dec 2021 14:04:22: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:04:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 14:04:26: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 14:04:26: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 14:04:26: #1 total tags in treatment: 35881508 INFO @ Wed, 08 Dec 2021 14:04:26: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:04:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:04:27: #1 tags after filtering in treatment: 35881508 INFO @ Wed, 08 Dec 2021 14:04:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:04:27: #1 finished! INFO @ Wed, 08 Dec 2021 14:04:27: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:04:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:04:30: #2 number of paired peaks: 7 WARNING @ Wed, 08 Dec 2021 14:04:30: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:04:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling