Job ID = 10165952 SRX = SRX7879253 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:24 45228743 reads; of these: 45228743 (100.00%) were unpaired; of these: 541047 (1.20%) aligned 0 times 36334657 (80.34%) aligned exactly 1 time 8353039 (18.47%) aligned >1 times 98.80% overall alignment rate Time searching: 00:10:24 Overall time: 00:10:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8806188 / 44687696 = 0.1971 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:35:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:35:42: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:35:42: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:35:48: 1000000 INFO @ Thu, 08 Oct 2020 20:35:54: 2000000 INFO @ Thu, 08 Oct 2020 20:36:00: 3000000 INFO @ Thu, 08 Oct 2020 20:36:06: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:36:12: 5000000 INFO @ Thu, 08 Oct 2020 20:36:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:36:13: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:36:13: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:36:19: 6000000 INFO @ Thu, 08 Oct 2020 20:36:20: 1000000 INFO @ Thu, 08 Oct 2020 20:36:26: 7000000 INFO @ Thu, 08 Oct 2020 20:36:28: 2000000 INFO @ Thu, 08 Oct 2020 20:36:33: 8000000 INFO @ Thu, 08 Oct 2020 20:36:35: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:36:40: 9000000 INFO @ Thu, 08 Oct 2020 20:36:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:36:43: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:36:43: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:36:43: 4000000 INFO @ Thu, 08 Oct 2020 20:36:48: 10000000 INFO @ Thu, 08 Oct 2020 20:36:51: 1000000 INFO @ Thu, 08 Oct 2020 20:36:51: 5000000 INFO @ Thu, 08 Oct 2020 20:36:55: 11000000 INFO @ Thu, 08 Oct 2020 20:36:58: 2000000 INFO @ Thu, 08 Oct 2020 20:36:58: 6000000 INFO @ Thu, 08 Oct 2020 20:37:03: 12000000 INFO @ Thu, 08 Oct 2020 20:37:06: 3000000 INFO @ Thu, 08 Oct 2020 20:37:06: 7000000 INFO @ Thu, 08 Oct 2020 20:37:10: 13000000 INFO @ Thu, 08 Oct 2020 20:37:13: 4000000 INFO @ Thu, 08 Oct 2020 20:37:13: 8000000 INFO @ Thu, 08 Oct 2020 20:37:18: 14000000 INFO @ Thu, 08 Oct 2020 20:37:21: 5000000 INFO @ Thu, 08 Oct 2020 20:37:21: 9000000 INFO @ Thu, 08 Oct 2020 20:37:25: 15000000 INFO @ Thu, 08 Oct 2020 20:37:28: 6000000 INFO @ Thu, 08 Oct 2020 20:37:28: 10000000 INFO @ Thu, 08 Oct 2020 20:37:33: 16000000 INFO @ Thu, 08 Oct 2020 20:37:36: 7000000 INFO @ Thu, 08 Oct 2020 20:37:36: 11000000 INFO @ Thu, 08 Oct 2020 20:37:40: 17000000 INFO @ Thu, 08 Oct 2020 20:37:43: 12000000 INFO @ Thu, 08 Oct 2020 20:37:43: 8000000 INFO @ Thu, 08 Oct 2020 20:37:48: 18000000 INFO @ Thu, 08 Oct 2020 20:37:51: 13000000 INFO @ Thu, 08 Oct 2020 20:37:51: 9000000 INFO @ Thu, 08 Oct 2020 20:37:55: 19000000 INFO @ Thu, 08 Oct 2020 20:37:58: 14000000 INFO @ Thu, 08 Oct 2020 20:37:58: 10000000 INFO @ Thu, 08 Oct 2020 20:38:03: 20000000 INFO @ Thu, 08 Oct 2020 20:38:06: 15000000 INFO @ Thu, 08 Oct 2020 20:38:06: 11000000 INFO @ Thu, 08 Oct 2020 20:38:10: 21000000 INFO @ Thu, 08 Oct 2020 20:38:13: 16000000 INFO @ Thu, 08 Oct 2020 20:38:13: 12000000 INFO @ Thu, 08 Oct 2020 20:38:18: 22000000 INFO @ Thu, 08 Oct 2020 20:38:21: 17000000 INFO @ Thu, 08 Oct 2020 20:38:21: 13000000 INFO @ Thu, 08 Oct 2020 20:38:25: 23000000 INFO @ Thu, 08 Oct 2020 20:38:28: 18000000 INFO @ Thu, 08 Oct 2020 20:38:28: 14000000 INFO @ Thu, 08 Oct 2020 20:38:33: 24000000 INFO @ Thu, 08 Oct 2020 20:38:36: 19000000 INFO @ Thu, 08 Oct 2020 20:38:36: 15000000 INFO @ Thu, 08 Oct 2020 20:38:40: 25000000 INFO @ Thu, 08 Oct 2020 20:38:43: 20000000 INFO @ Thu, 08 Oct 2020 20:38:43: 16000000 INFO @ Thu, 08 Oct 2020 20:38:48: 26000000 INFO @ Thu, 08 Oct 2020 20:38:51: 21000000 INFO @ Thu, 08 Oct 2020 20:38:51: 17000000 INFO @ Thu, 08 Oct 2020 20:38:56: 27000000 INFO @ Thu, 08 Oct 2020 20:38:58: 22000000 INFO @ Thu, 08 Oct 2020 20:38:59: 18000000 INFO @ Thu, 08 Oct 2020 20:39:03: 28000000 INFO @ Thu, 08 Oct 2020 20:39:06: 23000000 INFO @ Thu, 08 Oct 2020 20:39:06: 19000000 INFO @ Thu, 08 Oct 2020 20:39:11: 29000000 INFO @ Thu, 08 Oct 2020 20:39:14: 24000000 INFO @ Thu, 08 Oct 2020 20:39:14: 20000000 INFO @ Thu, 08 Oct 2020 20:39:19: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:39:22: 21000000 INFO @ Thu, 08 Oct 2020 20:39:22: 25000000 INFO @ Thu, 08 Oct 2020 20:39:26: 31000000 INFO @ Thu, 08 Oct 2020 20:39:29: 26000000 INFO @ Thu, 08 Oct 2020 20:39:29: 22000000 INFO @ Thu, 08 Oct 2020 20:39:34: 32000000 INFO @ Thu, 08 Oct 2020 20:39:37: 27000000 INFO @ Thu, 08 Oct 2020 20:39:37: 23000000 INFO @ Thu, 08 Oct 2020 20:39:42: 33000000 INFO @ Thu, 08 Oct 2020 20:39:45: 28000000 INFO @ Thu, 08 Oct 2020 20:39:45: 24000000 INFO @ Thu, 08 Oct 2020 20:39:49: 34000000 INFO @ Thu, 08 Oct 2020 20:39:52: 29000000 INFO @ Thu, 08 Oct 2020 20:39:52: 25000000 INFO @ Thu, 08 Oct 2020 20:39:57: 35000000 INFO @ Thu, 08 Oct 2020 20:40:00: 26000000 INFO @ Thu, 08 Oct 2020 20:40:00: 30000000 INFO @ Thu, 08 Oct 2020 20:40:03: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:40:03: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:40:03: #1 total tags in treatment: 35881508 INFO @ Thu, 08 Oct 2020 20:40:03: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:40:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:40:04: #1 tags after filtering in treatment: 35881508 INFO @ Thu, 08 Oct 2020 20:40:04: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:40:04: #1 finished! INFO @ Thu, 08 Oct 2020 20:40:04: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:40:04: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:40:06: #2 number of paired peaks: 7 WARNING @ Thu, 08 Oct 2020 20:40:06: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:40:06: Process for pairing-model is terminated! INFO @ Thu, 08 Oct 2020 20:40:07: 27000000 INFO @ Thu, 08 Oct 2020 20:40:08: 31000000 cut: /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:40:15: 28000000 INFO @ Thu, 08 Oct 2020 20:40:15: 32000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 20:40:22: 29000000 INFO @ Thu, 08 Oct 2020 20:40:23: 33000000 INFO @ Thu, 08 Oct 2020 20:40:29: 30000000 INFO @ Thu, 08 Oct 2020 20:40:30: 34000000 INFO @ Thu, 08 Oct 2020 20:40:37: 31000000 INFO @ Thu, 08 Oct 2020 20:40:37: 35000000 INFO @ Thu, 08 Oct 2020 20:40:44: 32000000 INFO @ Thu, 08 Oct 2020 20:40:45: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:40:45: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:40:45: #1 total tags in treatment: 35881508 INFO @ Thu, 08 Oct 2020 20:40:45: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:40:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:40:45: #1 tags after filtering in treatment: 35881508 INFO @ Thu, 08 Oct 2020 20:40:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:40:45: #1 finished! INFO @ Thu, 08 Oct 2020 20:40:45: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:40:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:40:48: #2 number of paired peaks: 7 WARNING @ Thu, 08 Oct 2020 20:40:48: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:40:48: Process for pairing-model is terminated! INFO @ Thu, 08 Oct 2020 20:40:50: 33000000 cut: /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:40:57: 34000000 INFO @ Thu, 08 Oct 2020 20:41:03: 35000000 INFO @ Thu, 08 Oct 2020 20:41:08: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:41:08: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:41:08: #1 total tags in treatment: 35881508 INFO @ Thu, 08 Oct 2020 20:41:08: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:41:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:41:09: #1 tags after filtering in treatment: 35881508 INFO @ Thu, 08 Oct 2020 20:41:09: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:41:09: #1 finished! INFO @ Thu, 08 Oct 2020 20:41:09: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:41:09: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:41:11: #2 number of paired peaks: 7 WARNING @ Thu, 08 Oct 2020 20:41:11: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:41:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879253/SRX7879253.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling