Job ID = 10165927 SRX = SRX7879250 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:36 45176912 reads; of these: 45176912 (100.00%) were unpaired; of these: 2583971 (5.72%) aligned 0 times 35588083 (78.77%) aligned exactly 1 time 7004858 (15.51%) aligned >1 times 94.28% overall alignment rate Time searching: 00:09:36 Overall time: 00:09:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7351158 / 42592941 = 0.1726 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:29:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:29:15: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:29:15: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:29:21: 1000000 INFO @ Thu, 08 Oct 2020 20:29:27: 2000000 INFO @ Thu, 08 Oct 2020 20:29:34: 3000000 INFO @ Thu, 08 Oct 2020 20:29:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:29:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:29:45: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:29:45: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:29:47: 5000000 INFO @ Thu, 08 Oct 2020 20:29:51: 1000000 INFO @ Thu, 08 Oct 2020 20:29:54: 6000000 INFO @ Thu, 08 Oct 2020 20:29:58: 2000000 INFO @ Thu, 08 Oct 2020 20:30:01: 7000000 INFO @ Thu, 08 Oct 2020 20:30:04: 3000000 INFO @ Thu, 08 Oct 2020 20:30:08: 8000000 INFO @ Thu, 08 Oct 2020 20:30:10: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:30:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:30:15: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:30:15: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:30:15: 9000000 INFO @ Thu, 08 Oct 2020 20:30:17: 5000000 INFO @ Thu, 08 Oct 2020 20:30:22: 1000000 INFO @ Thu, 08 Oct 2020 20:30:23: 10000000 INFO @ Thu, 08 Oct 2020 20:30:23: 6000000 INFO @ Thu, 08 Oct 2020 20:30:29: 2000000 INFO @ Thu, 08 Oct 2020 20:30:30: 7000000 INFO @ Thu, 08 Oct 2020 20:30:30: 11000000 INFO @ Thu, 08 Oct 2020 20:30:36: 3000000 INFO @ Thu, 08 Oct 2020 20:30:36: 8000000 INFO @ Thu, 08 Oct 2020 20:30:37: 12000000 INFO @ Thu, 08 Oct 2020 20:30:43: 9000000 INFO @ Thu, 08 Oct 2020 20:30:43: 4000000 INFO @ Thu, 08 Oct 2020 20:30:44: 13000000 INFO @ Thu, 08 Oct 2020 20:30:50: 10000000 INFO @ Thu, 08 Oct 2020 20:30:51: 5000000 INFO @ Thu, 08 Oct 2020 20:30:52: 14000000 INFO @ Thu, 08 Oct 2020 20:30:56: 11000000 INFO @ Thu, 08 Oct 2020 20:30:58: 6000000 INFO @ Thu, 08 Oct 2020 20:30:59: 15000000 INFO @ Thu, 08 Oct 2020 20:31:03: 12000000 INFO @ Thu, 08 Oct 2020 20:31:05: 7000000 INFO @ Thu, 08 Oct 2020 20:31:06: 16000000 INFO @ Thu, 08 Oct 2020 20:31:09: 13000000 INFO @ Thu, 08 Oct 2020 20:31:12: 8000000 INFO @ Thu, 08 Oct 2020 20:31:13: 17000000 INFO @ Thu, 08 Oct 2020 20:31:16: 14000000 INFO @ Thu, 08 Oct 2020 20:31:19: 9000000 INFO @ Thu, 08 Oct 2020 20:31:20: 18000000 INFO @ Thu, 08 Oct 2020 20:31:23: 15000000 INFO @ Thu, 08 Oct 2020 20:31:27: 10000000 INFO @ Thu, 08 Oct 2020 20:31:27: 19000000 INFO @ Thu, 08 Oct 2020 20:31:29: 16000000 INFO @ Thu, 08 Oct 2020 20:31:34: 11000000 INFO @ Thu, 08 Oct 2020 20:31:34: 20000000 INFO @ Thu, 08 Oct 2020 20:31:36: 17000000 INFO @ Thu, 08 Oct 2020 20:31:41: 21000000 INFO @ Thu, 08 Oct 2020 20:31:41: 12000000 INFO @ Thu, 08 Oct 2020 20:31:42: 18000000 INFO @ Thu, 08 Oct 2020 20:31:48: 22000000 INFO @ Thu, 08 Oct 2020 20:31:48: 13000000 INFO @ Thu, 08 Oct 2020 20:31:48: 19000000 INFO @ Thu, 08 Oct 2020 20:31:55: 20000000 INFO @ Thu, 08 Oct 2020 20:31:55: 23000000 INFO @ Thu, 08 Oct 2020 20:31:55: 14000000 INFO @ Thu, 08 Oct 2020 20:32:01: 21000000 INFO @ Thu, 08 Oct 2020 20:32:02: 24000000 INFO @ Thu, 08 Oct 2020 20:32:03: 15000000 INFO @ Thu, 08 Oct 2020 20:32:08: 22000000 INFO @ Thu, 08 Oct 2020 20:32:09: 25000000 INFO @ Thu, 08 Oct 2020 20:32:10: 16000000 INFO @ Thu, 08 Oct 2020 20:32:14: 23000000 INFO @ Thu, 08 Oct 2020 20:32:16: 26000000 INFO @ Thu, 08 Oct 2020 20:32:17: 17000000 INFO @ Thu, 08 Oct 2020 20:32:20: 24000000 INFO @ Thu, 08 Oct 2020 20:32:23: 27000000 INFO @ Thu, 08 Oct 2020 20:32:24: 18000000 INFO @ Thu, 08 Oct 2020 20:32:27: 25000000 INFO @ Thu, 08 Oct 2020 20:32:30: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:32:31: 19000000 INFO @ Thu, 08 Oct 2020 20:32:33: 26000000 INFO @ Thu, 08 Oct 2020 20:32:37: 29000000 INFO @ Thu, 08 Oct 2020 20:32:38: 20000000 INFO @ Thu, 08 Oct 2020 20:32:40: 27000000 INFO @ Thu, 08 Oct 2020 20:32:44: 30000000 INFO @ Thu, 08 Oct 2020 20:32:45: 21000000 INFO @ Thu, 08 Oct 2020 20:32:46: 28000000 INFO @ Thu, 08 Oct 2020 20:32:51: 31000000 INFO @ Thu, 08 Oct 2020 20:32:52: 22000000 INFO @ Thu, 08 Oct 2020 20:32:53: 29000000 INFO @ Thu, 08 Oct 2020 20:32:58: 32000000 INFO @ Thu, 08 Oct 2020 20:32:59: 30000000 INFO @ Thu, 08 Oct 2020 20:33:00: 23000000 INFO @ Thu, 08 Oct 2020 20:33:05: 31000000 INFO @ Thu, 08 Oct 2020 20:33:06: 33000000 INFO @ Thu, 08 Oct 2020 20:33:07: 24000000 INFO @ Thu, 08 Oct 2020 20:33:12: 32000000 INFO @ Thu, 08 Oct 2020 20:33:13: 34000000 INFO @ Thu, 08 Oct 2020 20:33:14: 25000000 INFO @ Thu, 08 Oct 2020 20:33:18: 33000000 INFO @ Thu, 08 Oct 2020 20:33:20: 35000000 INFO @ Thu, 08 Oct 2020 20:33:21: 26000000 INFO @ Thu, 08 Oct 2020 20:33:21: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:33:21: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:33:21: #1 total tags in treatment: 35241783 INFO @ Thu, 08 Oct 2020 20:33:21: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:33:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:33:22: #1 tags after filtering in treatment: 35241783 INFO @ Thu, 08 Oct 2020 20:33:22: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:33:22: #1 finished! INFO @ Thu, 08 Oct 2020 20:33:22: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:33:22: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:33:24: #2 number of paired peaks: 37 WARNING @ Thu, 08 Oct 2020 20:33:24: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:33:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:33:25: 34000000 INFO @ Thu, 08 Oct 2020 20:33:28: 27000000 INFO @ Thu, 08 Oct 2020 20:33:31: 35000000 INFO @ Thu, 08 Oct 2020 20:33:33: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:33:33: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:33:33: #1 total tags in treatment: 35241783 INFO @ Thu, 08 Oct 2020 20:33:33: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:33:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:33:33: #1 tags after filtering in treatment: 35241783 INFO @ Thu, 08 Oct 2020 20:33:33: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:33:33: #1 finished! INFO @ Thu, 08 Oct 2020 20:33:33: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:33:33: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:33:34: 28000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 20:33:35: #2 number of paired peaks: 37 WARNING @ Thu, 08 Oct 2020 20:33:35: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:33:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:33:41: 29000000 INFO @ Thu, 08 Oct 2020 20:33:48: 30000000 INFO @ Thu, 08 Oct 2020 20:33:54: 31000000 INFO @ Thu, 08 Oct 2020 20:34:01: 32000000 INFO @ Thu, 08 Oct 2020 20:34:07: 33000000 INFO @ Thu, 08 Oct 2020 20:34:13: 34000000 INFO @ Thu, 08 Oct 2020 20:34:20: 35000000 INFO @ Thu, 08 Oct 2020 20:34:21: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:34:21: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:34:21: #1 total tags in treatment: 35241783 INFO @ Thu, 08 Oct 2020 20:34:21: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:34:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:34:22: #1 tags after filtering in treatment: 35241783 INFO @ Thu, 08 Oct 2020 20:34:22: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:34:22: #1 finished! INFO @ Thu, 08 Oct 2020 20:34:22: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:34:22: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:34:24: #2 number of paired peaks: 37 WARNING @ Thu, 08 Oct 2020 20:34:24: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:34:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling