Job ID = 14157966 SRX = SRX7879250 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:47 45176912 reads; of these: 45176912 (100.00%) were unpaired; of these: 2583971 (5.72%) aligned 0 times 35588083 (78.77%) aligned exactly 1 time 7004858 (15.51%) aligned >1 times 94.28% overall alignment rate Time searching: 00:09:47 Overall time: 00:09:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7351158 / 42592941 = 0.1726 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 13:42:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 13:42:39: #1 read tag files... INFO @ Wed, 08 Dec 2021 13:42:39: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 13:42:45: 1000000 INFO @ Wed, 08 Dec 2021 13:42:50: 2000000 INFO @ Wed, 08 Dec 2021 13:42:56: 3000000 INFO @ Wed, 08 Dec 2021 13:43:01: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 13:43:07: 5000000 INFO @ Wed, 08 Dec 2021 13:43:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 13:43:08: #1 read tag files... INFO @ Wed, 08 Dec 2021 13:43:08: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 13:43:13: 6000000 INFO @ Wed, 08 Dec 2021 13:43:16: 1000000 INFO @ Wed, 08 Dec 2021 13:43:20: 7000000 INFO @ Wed, 08 Dec 2021 13:43:24: 2000000 INFO @ Wed, 08 Dec 2021 13:43:27: 8000000 INFO @ Wed, 08 Dec 2021 13:43:32: 3000000 INFO @ Wed, 08 Dec 2021 13:43:34: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 13:43:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 13:43:38: #1 read tag files... INFO @ Wed, 08 Dec 2021 13:43:38: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 13:43:40: 4000000 INFO @ Wed, 08 Dec 2021 13:43:40: 10000000 INFO @ Wed, 08 Dec 2021 13:43:45: 1000000 INFO @ Wed, 08 Dec 2021 13:43:48: 11000000 INFO @ Wed, 08 Dec 2021 13:43:48: 5000000 INFO @ Wed, 08 Dec 2021 13:43:52: 2000000 INFO @ Wed, 08 Dec 2021 13:43:55: 12000000 INFO @ Wed, 08 Dec 2021 13:43:56: 6000000 INFO @ Wed, 08 Dec 2021 13:43:59: 3000000 INFO @ Wed, 08 Dec 2021 13:44:02: 13000000 INFO @ Wed, 08 Dec 2021 13:44:04: 7000000 INFO @ Wed, 08 Dec 2021 13:44:06: 4000000 INFO @ Wed, 08 Dec 2021 13:44:09: 14000000 INFO @ Wed, 08 Dec 2021 13:44:12: 8000000 INFO @ Wed, 08 Dec 2021 13:44:14: 5000000 INFO @ Wed, 08 Dec 2021 13:44:16: 15000000 INFO @ Wed, 08 Dec 2021 13:44:20: 9000000 INFO @ Wed, 08 Dec 2021 13:44:21: 6000000 INFO @ Wed, 08 Dec 2021 13:44:23: 16000000 INFO @ Wed, 08 Dec 2021 13:44:27: 10000000 INFO @ Wed, 08 Dec 2021 13:44:28: 7000000 INFO @ Wed, 08 Dec 2021 13:44:30: 17000000 INFO @ Wed, 08 Dec 2021 13:44:35: 8000000 INFO @ Wed, 08 Dec 2021 13:44:35: 11000000 INFO @ Wed, 08 Dec 2021 13:44:37: 18000000 INFO @ Wed, 08 Dec 2021 13:44:43: 9000000 INFO @ Wed, 08 Dec 2021 13:44:43: 12000000 INFO @ Wed, 08 Dec 2021 13:44:45: 19000000 INFO @ Wed, 08 Dec 2021 13:44:50: 10000000 INFO @ Wed, 08 Dec 2021 13:44:51: 13000000 INFO @ Wed, 08 Dec 2021 13:44:52: 20000000 INFO @ Wed, 08 Dec 2021 13:44:57: 11000000 INFO @ Wed, 08 Dec 2021 13:44:59: 14000000 INFO @ Wed, 08 Dec 2021 13:44:59: 21000000 INFO @ Wed, 08 Dec 2021 13:45:04: 12000000 INFO @ Wed, 08 Dec 2021 13:45:06: 22000000 INFO @ Wed, 08 Dec 2021 13:45:07: 15000000 INFO @ Wed, 08 Dec 2021 13:45:11: 13000000 INFO @ Wed, 08 Dec 2021 13:45:13: 23000000 INFO @ Wed, 08 Dec 2021 13:45:14: 16000000 INFO @ Wed, 08 Dec 2021 13:45:19: 14000000 INFO @ Wed, 08 Dec 2021 13:45:21: 24000000 INFO @ Wed, 08 Dec 2021 13:45:22: 17000000 INFO @ Wed, 08 Dec 2021 13:45:26: 15000000 INFO @ Wed, 08 Dec 2021 13:45:28: 25000000 INFO @ Wed, 08 Dec 2021 13:45:30: 18000000 INFO @ Wed, 08 Dec 2021 13:45:33: 16000000 INFO @ Wed, 08 Dec 2021 13:45:35: 26000000 INFO @ Wed, 08 Dec 2021 13:45:37: 19000000 INFO @ Wed, 08 Dec 2021 13:45:40: 17000000 INFO @ Wed, 08 Dec 2021 13:45:42: 27000000 INFO @ Wed, 08 Dec 2021 13:45:45: 20000000 INFO @ Wed, 08 Dec 2021 13:45:47: 18000000 INFO @ Wed, 08 Dec 2021 13:45:49: 28000000 INFO @ Wed, 08 Dec 2021 13:45:53: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 13:45:55: 19000000 INFO @ Wed, 08 Dec 2021 13:45:57: 29000000 INFO @ Wed, 08 Dec 2021 13:46:00: 22000000 INFO @ Wed, 08 Dec 2021 13:46:02: 20000000 INFO @ Wed, 08 Dec 2021 13:46:04: 30000000 INFO @ Wed, 08 Dec 2021 13:46:08: 23000000 INFO @ Wed, 08 Dec 2021 13:46:09: 21000000 INFO @ Wed, 08 Dec 2021 13:46:11: 31000000 INFO @ Wed, 08 Dec 2021 13:46:15: 24000000 INFO @ Wed, 08 Dec 2021 13:46:16: 22000000 INFO @ Wed, 08 Dec 2021 13:46:18: 32000000 INFO @ Wed, 08 Dec 2021 13:46:23: 25000000 INFO @ Wed, 08 Dec 2021 13:46:24: 23000000 INFO @ Wed, 08 Dec 2021 13:46:26: 33000000 INFO @ Wed, 08 Dec 2021 13:46:30: 26000000 INFO @ Wed, 08 Dec 2021 13:46:31: 24000000 INFO @ Wed, 08 Dec 2021 13:46:33: 34000000 INFO @ Wed, 08 Dec 2021 13:46:37: 27000000 INFO @ Wed, 08 Dec 2021 13:46:38: 25000000 INFO @ Wed, 08 Dec 2021 13:46:40: 35000000 INFO @ Wed, 08 Dec 2021 13:46:42: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 13:46:42: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 13:46:42: #1 total tags in treatment: 35241783 INFO @ Wed, 08 Dec 2021 13:46:42: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 13:46:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 13:46:42: #1 tags after filtering in treatment: 35241783 INFO @ Wed, 08 Dec 2021 13:46:42: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 13:46:42: #1 finished! INFO @ Wed, 08 Dec 2021 13:46:42: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 13:46:42: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 13:46:44: #2 number of paired peaks: 37 WARNING @ Wed, 08 Dec 2021 13:46:44: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 13:46:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 13:46:45: 26000000 INFO @ Wed, 08 Dec 2021 13:46:45: 28000000 INFO @ Wed, 08 Dec 2021 13:46:52: 27000000 INFO @ Wed, 08 Dec 2021 13:46:52: 29000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 13:46:59: 28000000 INFO @ Wed, 08 Dec 2021 13:47:00: 30000000 INFO @ Wed, 08 Dec 2021 13:47:06: 29000000 INFO @ Wed, 08 Dec 2021 13:47:07: 31000000 INFO @ Wed, 08 Dec 2021 13:47:13: 30000000 INFO @ Wed, 08 Dec 2021 13:47:15: 32000000 INFO @ Wed, 08 Dec 2021 13:47:19: 31000000 INFO @ Wed, 08 Dec 2021 13:47:22: 33000000 INFO @ Wed, 08 Dec 2021 13:47:26: 32000000 INFO @ Wed, 08 Dec 2021 13:47:30: 34000000 INFO @ Wed, 08 Dec 2021 13:47:33: 33000000 INFO @ Wed, 08 Dec 2021 13:47:37: 35000000 INFO @ Wed, 08 Dec 2021 13:47:39: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 13:47:39: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 13:47:39: #1 total tags in treatment: 35241783 INFO @ Wed, 08 Dec 2021 13:47:39: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 13:47:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 13:47:40: #1 tags after filtering in treatment: 35241783 INFO @ Wed, 08 Dec 2021 13:47:40: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 13:47:40: #1 finished! INFO @ Wed, 08 Dec 2021 13:47:40: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 13:47:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 13:47:40: 34000000 INFO @ Wed, 08 Dec 2021 13:47:42: #2 number of paired peaks: 37 WARNING @ Wed, 08 Dec 2021 13:47:42: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 13:47:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 13:47:46: 35000000 INFO @ Wed, 08 Dec 2021 13:47:48: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 13:47:48: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 13:47:48: #1 total tags in treatment: 35241783 INFO @ Wed, 08 Dec 2021 13:47:48: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 13:47:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 13:47:49: #1 tags after filtering in treatment: 35241783 INFO @ Wed, 08 Dec 2021 13:47:49: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 13:47:49: #1 finished! INFO @ Wed, 08 Dec 2021 13:47:49: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 13:47:49: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 13:47:51: #2 number of paired peaks: 37 WARNING @ Wed, 08 Dec 2021 13:47:51: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 13:47:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879250/SRX7879250.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling