Job ID = 14157961 SRX = SRX7879249 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:23 39763228 reads; of these: 39763228 (100.00%) were unpaired; of these: 1219680 (3.07%) aligned 0 times 32311595 (81.26%) aligned exactly 1 time 6231953 (15.67%) aligned >1 times 96.93% overall alignment rate Time searching: 00:09:23 Overall time: 00:09:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6495072 / 38543548 = 0.1685 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 13:40:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 13:40:49: #1 read tag files... INFO @ Wed, 08 Dec 2021 13:40:49: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 13:40:58: 1000000 INFO @ Wed, 08 Dec 2021 13:41:07: 2000000 INFO @ Wed, 08 Dec 2021 13:41:15: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 13:41:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 13:41:19: #1 read tag files... INFO @ Wed, 08 Dec 2021 13:41:19: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 13:41:24: 4000000 INFO @ Wed, 08 Dec 2021 13:41:28: 1000000 INFO @ Wed, 08 Dec 2021 13:41:32: 5000000 INFO @ Wed, 08 Dec 2021 13:41:36: 2000000 INFO @ Wed, 08 Dec 2021 13:41:41: 6000000 INFO @ Wed, 08 Dec 2021 13:41:43: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 13:41:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 13:41:49: #1 read tag files... INFO @ Wed, 08 Dec 2021 13:41:49: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 13:41:49: 7000000 INFO @ Wed, 08 Dec 2021 13:41:52: 4000000 INFO @ Wed, 08 Dec 2021 13:41:58: 8000000 INFO @ Wed, 08 Dec 2021 13:41:59: 1000000 INFO @ Wed, 08 Dec 2021 13:42:00: 5000000 INFO @ Wed, 08 Dec 2021 13:42:06: 9000000 INFO @ Wed, 08 Dec 2021 13:42:08: 6000000 INFO @ Wed, 08 Dec 2021 13:42:08: 2000000 INFO @ Wed, 08 Dec 2021 13:42:15: 10000000 INFO @ Wed, 08 Dec 2021 13:42:16: 7000000 INFO @ Wed, 08 Dec 2021 13:42:17: 3000000 INFO @ Wed, 08 Dec 2021 13:42:24: 11000000 INFO @ Wed, 08 Dec 2021 13:42:24: 8000000 INFO @ Wed, 08 Dec 2021 13:42:27: 4000000 INFO @ Wed, 08 Dec 2021 13:42:32: 12000000 INFO @ Wed, 08 Dec 2021 13:42:33: 9000000 INFO @ Wed, 08 Dec 2021 13:42:36: 5000000 INFO @ Wed, 08 Dec 2021 13:42:41: 10000000 INFO @ Wed, 08 Dec 2021 13:42:41: 13000000 INFO @ Wed, 08 Dec 2021 13:42:45: 6000000 INFO @ Wed, 08 Dec 2021 13:42:48: 11000000 INFO @ Wed, 08 Dec 2021 13:42:49: 14000000 INFO @ Wed, 08 Dec 2021 13:42:54: 7000000 INFO @ Wed, 08 Dec 2021 13:42:57: 12000000 INFO @ Wed, 08 Dec 2021 13:42:58: 15000000 INFO @ Wed, 08 Dec 2021 13:43:02: 8000000 INFO @ Wed, 08 Dec 2021 13:43:05: 13000000 INFO @ Wed, 08 Dec 2021 13:43:07: 16000000 INFO @ Wed, 08 Dec 2021 13:43:11: 9000000 INFO @ Wed, 08 Dec 2021 13:43:13: 14000000 INFO @ Wed, 08 Dec 2021 13:43:15: 17000000 INFO @ Wed, 08 Dec 2021 13:43:20: 10000000 INFO @ Wed, 08 Dec 2021 13:43:21: 15000000 INFO @ Wed, 08 Dec 2021 13:43:24: 18000000 INFO @ Wed, 08 Dec 2021 13:43:29: 11000000 INFO @ Wed, 08 Dec 2021 13:43:29: 16000000 INFO @ Wed, 08 Dec 2021 13:43:32: 19000000 INFO @ Wed, 08 Dec 2021 13:43:37: 17000000 INFO @ Wed, 08 Dec 2021 13:43:38: 12000000 INFO @ Wed, 08 Dec 2021 13:43:41: 20000000 INFO @ Wed, 08 Dec 2021 13:43:45: 18000000 INFO @ Wed, 08 Dec 2021 13:43:47: 13000000 INFO @ Wed, 08 Dec 2021 13:43:50: 21000000 INFO @ Wed, 08 Dec 2021 13:43:53: 19000000 INFO @ Wed, 08 Dec 2021 13:43:56: 14000000 INFO @ Wed, 08 Dec 2021 13:43:58: 22000000 INFO @ Wed, 08 Dec 2021 13:44:01: 20000000 INFO @ Wed, 08 Dec 2021 13:44:04: 15000000 INFO @ Wed, 08 Dec 2021 13:44:06: 23000000 INFO @ Wed, 08 Dec 2021 13:44:08: 21000000 INFO @ Wed, 08 Dec 2021 13:44:13: 16000000 INFO @ Wed, 08 Dec 2021 13:44:15: 24000000 INFO @ Wed, 08 Dec 2021 13:44:16: 22000000 INFO @ Wed, 08 Dec 2021 13:44:22: 17000000 INFO @ Wed, 08 Dec 2021 13:44:23: 23000000 INFO @ Wed, 08 Dec 2021 13:44:23: 25000000 INFO @ Wed, 08 Dec 2021 13:44:31: 18000000 INFO @ Wed, 08 Dec 2021 13:44:31: 24000000 INFO @ Wed, 08 Dec 2021 13:44:32: 26000000 INFO @ Wed, 08 Dec 2021 13:44:38: 25000000 INFO @ Wed, 08 Dec 2021 13:44:39: 19000000 INFO @ Wed, 08 Dec 2021 13:44:40: 27000000 INFO @ Wed, 08 Dec 2021 13:44:46: 26000000 INFO @ Wed, 08 Dec 2021 13:44:48: 20000000 INFO @ Wed, 08 Dec 2021 13:44:49: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 13:44:53: 27000000 INFO @ Wed, 08 Dec 2021 13:44:57: 21000000 INFO @ Wed, 08 Dec 2021 13:44:57: 29000000 INFO @ Wed, 08 Dec 2021 13:45:01: 28000000 INFO @ Wed, 08 Dec 2021 13:45:06: 30000000 INFO @ Wed, 08 Dec 2021 13:45:06: 22000000 INFO @ Wed, 08 Dec 2021 13:45:09: 29000000 INFO @ Wed, 08 Dec 2021 13:45:14: 31000000 INFO @ Wed, 08 Dec 2021 13:45:15: 23000000 INFO @ Wed, 08 Dec 2021 13:45:16: 30000000 INFO @ Wed, 08 Dec 2021 13:45:23: 32000000 INFO @ Wed, 08 Dec 2021 13:45:23: 31000000 INFO @ Wed, 08 Dec 2021 13:45:23: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 13:45:23: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 13:45:23: #1 total tags in treatment: 32048476 INFO @ Wed, 08 Dec 2021 13:45:23: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 13:45:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 13:45:24: 24000000 INFO @ Wed, 08 Dec 2021 13:45:24: #1 tags after filtering in treatment: 32048476 INFO @ Wed, 08 Dec 2021 13:45:24: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 13:45:24: #1 finished! INFO @ Wed, 08 Dec 2021 13:45:24: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 13:45:24: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 13:45:27: #2 number of paired peaks: 65 WARNING @ Wed, 08 Dec 2021 13:45:27: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 13:45:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 13:45:31: 32000000 INFO @ Wed, 08 Dec 2021 13:45:31: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 13:45:31: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 13:45:31: #1 total tags in treatment: 32048476 INFO @ Wed, 08 Dec 2021 13:45:31: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 13:45:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 13:45:32: #1 tags after filtering in treatment: 32048476 INFO @ Wed, 08 Dec 2021 13:45:32: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 13:45:32: #1 finished! INFO @ Wed, 08 Dec 2021 13:45:32: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 13:45:32: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 13:45:32: 25000000 INFO @ Wed, 08 Dec 2021 13:45:34: #2 number of paired peaks: 65 WARNING @ Wed, 08 Dec 2021 13:45:34: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 13:45:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 13:45:41: 26000000 INFO @ Wed, 08 Dec 2021 13:45:49: 27000000 INFO @ Wed, 08 Dec 2021 13:45:58: 28000000 INFO @ Wed, 08 Dec 2021 13:46:06: 29000000 INFO @ Wed, 08 Dec 2021 13:46:15: 30000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 13:46:23: 31000000 INFO @ Wed, 08 Dec 2021 13:46:32: 32000000 INFO @ Wed, 08 Dec 2021 13:46:32: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 13:46:32: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 13:46:32: #1 total tags in treatment: 32048476 INFO @ Wed, 08 Dec 2021 13:46:32: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 13:46:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 13:46:33: #1 tags after filtering in treatment: 32048476 INFO @ Wed, 08 Dec 2021 13:46:33: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 13:46:33: #1 finished! INFO @ Wed, 08 Dec 2021 13:46:33: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 13:46:33: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 13:46:36: #2 number of paired peaks: 65 WARNING @ Wed, 08 Dec 2021 13:46:36: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 13:46:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling