Job ID = 10165922 SRX = SRX7879249 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:35 39763228 reads; of these: 39763228 (100.00%) were unpaired; of these: 1219680 (3.07%) aligned 0 times 32311595 (81.26%) aligned exactly 1 time 6231953 (15.67%) aligned >1 times 96.93% overall alignment rate Time searching: 00:08:35 Overall time: 00:08:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6495072 / 38543548 = 0.1685 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:27:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:27:02: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:27:02: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:27:08: 1000000 INFO @ Thu, 08 Oct 2020 20:27:14: 2000000 INFO @ Thu, 08 Oct 2020 20:27:21: 3000000 INFO @ Thu, 08 Oct 2020 20:27:27: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:27:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:27:32: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:27:32: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:27:33: 5000000 INFO @ Thu, 08 Oct 2020 20:27:38: 1000000 INFO @ Thu, 08 Oct 2020 20:27:40: 6000000 INFO @ Thu, 08 Oct 2020 20:27:45: 2000000 INFO @ Thu, 08 Oct 2020 20:27:47: 7000000 INFO @ Thu, 08 Oct 2020 20:27:52: 3000000 INFO @ Thu, 08 Oct 2020 20:27:54: 8000000 INFO @ Thu, 08 Oct 2020 20:27:58: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:28:01: 9000000 INFO @ Thu, 08 Oct 2020 20:28:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:28:02: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:28:02: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:28:05: 5000000 INFO @ Thu, 08 Oct 2020 20:28:08: 10000000 INFO @ Thu, 08 Oct 2020 20:28:09: 1000000 INFO @ Thu, 08 Oct 2020 20:28:12: 6000000 INFO @ Thu, 08 Oct 2020 20:28:15: 11000000 INFO @ Thu, 08 Oct 2020 20:28:16: 2000000 INFO @ Thu, 08 Oct 2020 20:28:19: 7000000 INFO @ Thu, 08 Oct 2020 20:28:23: 12000000 INFO @ Thu, 08 Oct 2020 20:28:24: 3000000 INFO @ Thu, 08 Oct 2020 20:28:26: 8000000 INFO @ Thu, 08 Oct 2020 20:28:30: 13000000 INFO @ Thu, 08 Oct 2020 20:28:31: 4000000 INFO @ Thu, 08 Oct 2020 20:28:33: 9000000 INFO @ Thu, 08 Oct 2020 20:28:37: 14000000 INFO @ Thu, 08 Oct 2020 20:28:37: 5000000 INFO @ Thu, 08 Oct 2020 20:28:39: 10000000 INFO @ Thu, 08 Oct 2020 20:28:44: 15000000 INFO @ Thu, 08 Oct 2020 20:28:44: 6000000 INFO @ Thu, 08 Oct 2020 20:28:46: 11000000 INFO @ Thu, 08 Oct 2020 20:28:51: 16000000 INFO @ Thu, 08 Oct 2020 20:28:51: 7000000 INFO @ Thu, 08 Oct 2020 20:28:53: 12000000 INFO @ Thu, 08 Oct 2020 20:28:58: 17000000 INFO @ Thu, 08 Oct 2020 20:28:58: 8000000 INFO @ Thu, 08 Oct 2020 20:29:00: 13000000 INFO @ Thu, 08 Oct 2020 20:29:05: 9000000 INFO @ Thu, 08 Oct 2020 20:29:05: 18000000 INFO @ Thu, 08 Oct 2020 20:29:07: 14000000 INFO @ Thu, 08 Oct 2020 20:29:12: 10000000 INFO @ Thu, 08 Oct 2020 20:29:13: 19000000 INFO @ Thu, 08 Oct 2020 20:29:14: 15000000 INFO @ Thu, 08 Oct 2020 20:29:19: 11000000 INFO @ Thu, 08 Oct 2020 20:29:20: 20000000 INFO @ Thu, 08 Oct 2020 20:29:21: 16000000 INFO @ Thu, 08 Oct 2020 20:29:26: 12000000 INFO @ Thu, 08 Oct 2020 20:29:27: 21000000 INFO @ Thu, 08 Oct 2020 20:29:28: 17000000 INFO @ Thu, 08 Oct 2020 20:29:33: 13000000 INFO @ Thu, 08 Oct 2020 20:29:34: 22000000 INFO @ Thu, 08 Oct 2020 20:29:35: 18000000 INFO @ Thu, 08 Oct 2020 20:29:40: 14000000 INFO @ Thu, 08 Oct 2020 20:29:41: 23000000 INFO @ Thu, 08 Oct 2020 20:29:42: 19000000 INFO @ Thu, 08 Oct 2020 20:29:47: 15000000 INFO @ Thu, 08 Oct 2020 20:29:48: 24000000 INFO @ Thu, 08 Oct 2020 20:29:49: 20000000 INFO @ Thu, 08 Oct 2020 20:29:54: 16000000 INFO @ Thu, 08 Oct 2020 20:29:55: 25000000 INFO @ Thu, 08 Oct 2020 20:29:56: 21000000 INFO @ Thu, 08 Oct 2020 20:30:01: 17000000 INFO @ Thu, 08 Oct 2020 20:30:02: 26000000 INFO @ Thu, 08 Oct 2020 20:30:03: 22000000 INFO @ Thu, 08 Oct 2020 20:30:08: 18000000 INFO @ Thu, 08 Oct 2020 20:30:10: 23000000 INFO @ Thu, 08 Oct 2020 20:30:10: 27000000 INFO @ Thu, 08 Oct 2020 20:30:15: 19000000 INFO @ Thu, 08 Oct 2020 20:30:16: 24000000 INFO @ Thu, 08 Oct 2020 20:30:17: 28000000 INFO @ Thu, 08 Oct 2020 20:30:22: 20000000 INFO @ Thu, 08 Oct 2020 20:30:23: 25000000 INFO @ Thu, 08 Oct 2020 20:30:24: 29000000 INFO @ Thu, 08 Oct 2020 20:30:29: 21000000 INFO @ Thu, 08 Oct 2020 20:30:30: 26000000 INFO @ Thu, 08 Oct 2020 20:30:31: 30000000 INFO @ Thu, 08 Oct 2020 20:30:37: 22000000 INFO @ Thu, 08 Oct 2020 20:30:37: 27000000 INFO @ Thu, 08 Oct 2020 20:30:38: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:30:44: 23000000 INFO @ Thu, 08 Oct 2020 20:30:44: 28000000 INFO @ Thu, 08 Oct 2020 20:30:45: 32000000 INFO @ Thu, 08 Oct 2020 20:30:46: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:30:46: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:30:46: #1 total tags in treatment: 32048476 INFO @ Thu, 08 Oct 2020 20:30:46: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:30:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:30:46: #1 tags after filtering in treatment: 32048476 INFO @ Thu, 08 Oct 2020 20:30:46: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:30:46: #1 finished! INFO @ Thu, 08 Oct 2020 20:30:46: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:30:46: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:30:48: #2 number of paired peaks: 65 WARNING @ Thu, 08 Oct 2020 20:30:48: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:30:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:30:51: 24000000 INFO @ Thu, 08 Oct 2020 20:30:51: 29000000 INFO @ Thu, 08 Oct 2020 20:30:57: 25000000 INFO @ Thu, 08 Oct 2020 20:30:58: 30000000 INFO @ Thu, 08 Oct 2020 20:31:04: 31000000 INFO @ Thu, 08 Oct 2020 20:31:04: 26000000 INFO @ Thu, 08 Oct 2020 20:31:11: 32000000 INFO @ Thu, 08 Oct 2020 20:31:11: 27000000 INFO @ Thu, 08 Oct 2020 20:31:12: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:31:12: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:31:12: #1 total tags in treatment: 32048476 INFO @ Thu, 08 Oct 2020 20:31:12: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:31:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:31:12: #1 tags after filtering in treatment: 32048476 INFO @ Thu, 08 Oct 2020 20:31:12: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:31:12: #1 finished! INFO @ Thu, 08 Oct 2020 20:31:12: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:31:12: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:31:14: #2 number of paired peaks: 65 WARNING @ Thu, 08 Oct 2020 20:31:14: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:31:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:31:18: 28000000 INFO @ Thu, 08 Oct 2020 20:31:24: 29000000 INFO @ Thu, 08 Oct 2020 20:31:31: 30000000 INFO @ Thu, 08 Oct 2020 20:31:37: 31000000 INFO @ Thu, 08 Oct 2020 20:31:43: 32000000 INFO @ Thu, 08 Oct 2020 20:31:44: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:31:44: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:31:44: #1 total tags in treatment: 32048476 INFO @ Thu, 08 Oct 2020 20:31:44: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:31:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:31:44: #1 tags after filtering in treatment: 32048476 INFO @ Thu, 08 Oct 2020 20:31:44: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:31:44: #1 finished! INFO @ Thu, 08 Oct 2020 20:31:44: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:31:44: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:31:46: #2 number of paired peaks: 65 WARNING @ Thu, 08 Oct 2020 20:31:46: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:31:46: Process for pairing-model is terminated! BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879249/SRX7879249.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling