Job ID = 12265223 SRX = SRX7246260 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:07 55460313 reads; of these: 55460313 (100.00%) were unpaired; of these: 2335386 (4.21%) aligned 0 times 42147884 (76.00%) aligned exactly 1 time 10977043 (19.79%) aligned >1 times 95.79% overall alignment rate Time searching: 00:13:07 Overall time: 00:13:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 30162439 / 53124927 = 0.5678 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:36:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:36:55: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:36:55: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:37:01: 1000000 INFO @ Sat, 03 Apr 2021 06:37:07: 2000000 INFO @ Sat, 03 Apr 2021 06:37:13: 3000000 INFO @ Sat, 03 Apr 2021 06:37:19: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:37:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:37:25: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:37:25: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:37:26: 5000000 INFO @ Sat, 03 Apr 2021 06:37:31: 1000000 INFO @ Sat, 03 Apr 2021 06:37:32: 6000000 INFO @ Sat, 03 Apr 2021 06:37:37: 2000000 INFO @ Sat, 03 Apr 2021 06:37:38: 7000000 INFO @ Sat, 03 Apr 2021 06:37:43: 3000000 INFO @ Sat, 03 Apr 2021 06:37:45: 8000000 INFO @ Sat, 03 Apr 2021 06:37:49: 4000000 INFO @ Sat, 03 Apr 2021 06:37:51: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:37:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:37:55: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:37:55: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:37:55: 5000000 INFO @ Sat, 03 Apr 2021 06:37:58: 10000000 INFO @ Sat, 03 Apr 2021 06:38:01: 1000000 INFO @ Sat, 03 Apr 2021 06:38:02: 6000000 INFO @ Sat, 03 Apr 2021 06:38:05: 11000000 INFO @ Sat, 03 Apr 2021 06:38:07: 2000000 INFO @ Sat, 03 Apr 2021 06:38:09: 7000000 INFO @ Sat, 03 Apr 2021 06:38:12: 12000000 INFO @ Sat, 03 Apr 2021 06:38:13: 3000000 INFO @ Sat, 03 Apr 2021 06:38:15: 8000000 INFO @ Sat, 03 Apr 2021 06:38:19: 13000000 INFO @ Sat, 03 Apr 2021 06:38:20: 4000000 INFO @ Sat, 03 Apr 2021 06:38:21: 9000000 INFO @ Sat, 03 Apr 2021 06:38:26: 14000000 INFO @ Sat, 03 Apr 2021 06:38:26: 5000000 INFO @ Sat, 03 Apr 2021 06:38:27: 10000000 INFO @ Sat, 03 Apr 2021 06:38:33: 15000000 INFO @ Sat, 03 Apr 2021 06:38:33: 6000000 INFO @ Sat, 03 Apr 2021 06:38:33: 11000000 INFO @ Sat, 03 Apr 2021 06:38:39: 7000000 INFO @ Sat, 03 Apr 2021 06:38:39: 16000000 INFO @ Sat, 03 Apr 2021 06:38:40: 12000000 INFO @ Sat, 03 Apr 2021 06:38:45: 8000000 INFO @ Sat, 03 Apr 2021 06:38:46: 17000000 INFO @ Sat, 03 Apr 2021 06:38:46: 13000000 INFO @ Sat, 03 Apr 2021 06:38:52: 9000000 INFO @ Sat, 03 Apr 2021 06:38:52: 18000000 INFO @ Sat, 03 Apr 2021 06:38:53: 14000000 INFO @ Sat, 03 Apr 2021 06:38:58: 10000000 INFO @ Sat, 03 Apr 2021 06:38:59: 15000000 INFO @ Sat, 03 Apr 2021 06:38:59: 19000000 INFO @ Sat, 03 Apr 2021 06:39:04: 11000000 INFO @ Sat, 03 Apr 2021 06:39:05: 16000000 INFO @ Sat, 03 Apr 2021 06:39:06: 20000000 INFO @ Sat, 03 Apr 2021 06:39:10: 12000000 INFO @ Sat, 03 Apr 2021 06:39:12: 17000000 INFO @ Sat, 03 Apr 2021 06:39:13: 21000000 INFO @ Sat, 03 Apr 2021 06:39:17: 13000000 INFO @ Sat, 03 Apr 2021 06:39:18: 18000000 INFO @ Sat, 03 Apr 2021 06:39:19: 22000000 INFO @ Sat, 03 Apr 2021 06:39:23: 14000000 INFO @ Sat, 03 Apr 2021 06:39:24: 19000000 INFO @ Sat, 03 Apr 2021 06:39:26: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 06:39:26: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 06:39:26: #1 total tags in treatment: 22962488 INFO @ Sat, 03 Apr 2021 06:39:26: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:39:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:39:26: #1 tags after filtering in treatment: 22962488 INFO @ Sat, 03 Apr 2021 06:39:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:39:26: #1 finished! INFO @ Sat, 03 Apr 2021 06:39:26: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:39:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:39:28: #2 number of paired peaks: 65 WARNING @ Sat, 03 Apr 2021 06:39:28: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 06:39:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 06:39:30: 15000000 INFO @ Sat, 03 Apr 2021 06:39:30: 20000000 INFO @ Sat, 03 Apr 2021 06:39:36: 16000000 INFO @ Sat, 03 Apr 2021 06:39:36: 21000000 INFO @ Sat, 03 Apr 2021 06:39:41: 17000000 INFO @ Sat, 03 Apr 2021 06:39:42: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 06:39:47: 18000000 INFO @ Sat, 03 Apr 2021 06:39:48: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 06:39:48: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 06:39:48: #1 total tags in treatment: 22962488 INFO @ Sat, 03 Apr 2021 06:39:48: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:39:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:39:48: #1 tags after filtering in treatment: 22962488 INFO @ Sat, 03 Apr 2021 06:39:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:39:48: #1 finished! INFO @ Sat, 03 Apr 2021 06:39:48: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:39:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:39:50: #2 number of paired peaks: 65 WARNING @ Sat, 03 Apr 2021 06:39:50: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 06:39:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 06:39:53: 19000000 INFO @ Sat, 03 Apr 2021 06:39:58: 20000000 INFO @ Sat, 03 Apr 2021 06:40:04: 21000000 INFO @ Sat, 03 Apr 2021 06:40:09: 22000000 INFO @ Sat, 03 Apr 2021 06:40:15: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 06:40:15: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 06:40:15: #1 total tags in treatment: 22962488 INFO @ Sat, 03 Apr 2021 06:40:15: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:40:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:40:15: #1 tags after filtering in treatment: 22962488 INFO @ Sat, 03 Apr 2021 06:40:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:40:15: #1 finished! INFO @ Sat, 03 Apr 2021 06:40:15: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:40:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:40:17: #2 number of paired peaks: 65 WARNING @ Sat, 03 Apr 2021 06:40:17: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 06:40:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7246260/SRX7246260.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。