Job ID = 6368882 SRX = SRX6619583 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-16T00:58:00 prefetch.2.10.7: 1) Downloading 'SRR9866026'... 2020-06-16T00:58:00 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T01:18:03 prefetch.2.10.7: HTTPS download succeed 2020-06-16T01:18:03 prefetch.2.10.7: 1) 'SRR9866026' was downloaded successfully 2020-06-16T01:18:03 prefetch.2.10.7: 'SRR9866026' has 0 unresolved dependencies Read 43555027 spots for SRR9866026/SRR9866026.sra Written 43555027 spots for SRR9866026/SRR9866026.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:21:38 43555027 reads; of these: 43555027 (100.00%) were paired; of these: 27937513 (64.14%) aligned concordantly 0 times 12830720 (29.46%) aligned concordantly exactly 1 time 2786794 (6.40%) aligned concordantly >1 times ---- 27937513 pairs aligned concordantly 0 times; of these: 14025004 (50.20%) aligned discordantly 1 time ---- 13912509 pairs aligned 0 times concordantly or discordantly; of these: 27825018 mates make up the pairs; of these: 24161246 (86.83%) aligned 0 times 1278939 (4.60%) aligned exactly 1 time 2384833 (8.57%) aligned >1 times 72.26% overall alignment rate Time searching: 01:21:39 Overall time: 01:21:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4682442 / 29065309 = 0.1611 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 12:16:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 12:16:24: #1 read tag files... INFO @ Tue, 16 Jun 2020 12:16:24: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 12:16:33: 1000000 INFO @ Tue, 16 Jun 2020 12:16:41: 2000000 INFO @ Tue, 16 Jun 2020 12:16:49: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 12:16:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 12:16:54: #1 read tag files... INFO @ Tue, 16 Jun 2020 12:16:54: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 12:16:57: 4000000 INFO @ Tue, 16 Jun 2020 12:17:03: 1000000 INFO @ Tue, 16 Jun 2020 12:17:07: 5000000 INFO @ Tue, 16 Jun 2020 12:17:12: 2000000 INFO @ Tue, 16 Jun 2020 12:17:16: 6000000 INFO @ Tue, 16 Jun 2020 12:17:22: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 12:17:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 12:17:24: #1 read tag files... INFO @ Tue, 16 Jun 2020 12:17:24: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 12:17:26: 7000000 INFO @ Tue, 16 Jun 2020 12:17:32: 4000000 INFO @ Tue, 16 Jun 2020 12:17:36: 1000000 INFO @ Tue, 16 Jun 2020 12:17:36: 8000000 INFO @ Tue, 16 Jun 2020 12:17:43: 5000000 INFO @ Tue, 16 Jun 2020 12:17:47: 9000000 INFO @ Tue, 16 Jun 2020 12:17:48: 2000000 INFO @ Tue, 16 Jun 2020 12:17:53: 6000000 INFO @ Tue, 16 Jun 2020 12:17:58: 10000000 INFO @ Tue, 16 Jun 2020 12:18:00: 3000000 INFO @ Tue, 16 Jun 2020 12:18:04: 7000000 INFO @ Tue, 16 Jun 2020 12:18:08: 11000000 INFO @ Tue, 16 Jun 2020 12:18:11: 4000000 INFO @ Tue, 16 Jun 2020 12:18:15: 8000000 INFO @ Tue, 16 Jun 2020 12:18:19: 12000000 INFO @ Tue, 16 Jun 2020 12:18:23: 5000000 INFO @ Tue, 16 Jun 2020 12:18:26: 9000000 INFO @ Tue, 16 Jun 2020 12:18:30: 13000000 INFO @ Tue, 16 Jun 2020 12:18:34: 6000000 INFO @ Tue, 16 Jun 2020 12:18:36: 10000000 INFO @ Tue, 16 Jun 2020 12:18:40: 14000000 INFO @ Tue, 16 Jun 2020 12:18:46: 7000000 INFO @ Tue, 16 Jun 2020 12:18:47: 11000000 INFO @ Tue, 16 Jun 2020 12:18:51: 15000000 INFO @ Tue, 16 Jun 2020 12:18:58: 12000000 INFO @ Tue, 16 Jun 2020 12:18:58: 8000000 INFO @ Tue, 16 Jun 2020 12:19:02: 16000000 INFO @ Tue, 16 Jun 2020 12:19:09: 13000000 INFO @ Tue, 16 Jun 2020 12:19:10: 9000000 INFO @ Tue, 16 Jun 2020 12:19:13: 17000000 INFO @ Tue, 16 Jun 2020 12:19:19: 14000000 INFO @ Tue, 16 Jun 2020 12:19:22: 10000000 INFO @ Tue, 16 Jun 2020 12:19:24: 18000000 INFO @ Tue, 16 Jun 2020 12:19:30: 15000000 INFO @ Tue, 16 Jun 2020 12:19:34: 11000000 INFO @ Tue, 16 Jun 2020 12:19:35: 19000000 INFO @ Tue, 16 Jun 2020 12:19:41: 16000000 INFO @ Tue, 16 Jun 2020 12:19:46: 12000000 INFO @ Tue, 16 Jun 2020 12:19:46: 20000000 INFO @ Tue, 16 Jun 2020 12:19:52: 17000000 INFO @ Tue, 16 Jun 2020 12:19:56: 21000000 INFO @ Tue, 16 Jun 2020 12:19:57: 13000000 INFO @ Tue, 16 Jun 2020 12:20:03: 18000000 INFO @ Tue, 16 Jun 2020 12:20:07: 22000000 INFO @ Tue, 16 Jun 2020 12:20:09: 14000000 INFO @ Tue, 16 Jun 2020 12:20:14: 19000000 INFO @ Tue, 16 Jun 2020 12:20:18: 23000000 INFO @ Tue, 16 Jun 2020 12:20:20: 15000000 INFO @ Tue, 16 Jun 2020 12:20:24: 20000000 INFO @ Tue, 16 Jun 2020 12:20:28: 24000000 INFO @ Tue, 16 Jun 2020 12:20:32: 16000000 INFO @ Tue, 16 Jun 2020 12:20:35: 21000000 INFO @ Tue, 16 Jun 2020 12:20:39: 25000000 INFO @ Tue, 16 Jun 2020 12:20:43: 17000000 INFO @ Tue, 16 Jun 2020 12:20:45: 22000000 INFO @ Tue, 16 Jun 2020 12:20:49: 26000000 INFO @ Tue, 16 Jun 2020 12:20:55: 18000000 INFO @ Tue, 16 Jun 2020 12:20:56: 23000000 INFO @ Tue, 16 Jun 2020 12:21:00: 27000000 INFO @ Tue, 16 Jun 2020 12:21:06: 24000000 INFO @ Tue, 16 Jun 2020 12:21:07: 19000000 INFO @ Tue, 16 Jun 2020 12:21:10: 28000000 INFO @ Tue, 16 Jun 2020 12:21:17: 25000000 INFO @ Tue, 16 Jun 2020 12:21:19: 20000000 INFO @ Tue, 16 Jun 2020 12:21:20: 29000000 INFO @ Tue, 16 Jun 2020 12:21:27: 26000000 INFO @ Tue, 16 Jun 2020 12:21:30: 21000000 INFO @ Tue, 16 Jun 2020 12:21:31: 30000000 INFO @ Tue, 16 Jun 2020 12:21:37: 27000000 INFO @ Tue, 16 Jun 2020 12:21:41: 31000000 INFO @ Tue, 16 Jun 2020 12:21:42: 22000000 INFO @ Tue, 16 Jun 2020 12:21:48: 28000000 INFO @ Tue, 16 Jun 2020 12:21:52: 32000000 INFO @ Tue, 16 Jun 2020 12:21:53: 23000000 INFO @ Tue, 16 Jun 2020 12:21:58: 29000000 INFO @ Tue, 16 Jun 2020 12:22:02: 33000000 INFO @ Tue, 16 Jun 2020 12:22:05: 24000000 INFO @ Tue, 16 Jun 2020 12:22:08: 30000000 INFO @ Tue, 16 Jun 2020 12:22:12: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 12:22:16: 25000000 INFO @ Tue, 16 Jun 2020 12:22:19: 31000000 INFO @ Tue, 16 Jun 2020 12:22:23: 35000000 INFO @ Tue, 16 Jun 2020 12:22:27: 26000000 INFO @ Tue, 16 Jun 2020 12:22:29: 32000000 INFO @ Tue, 16 Jun 2020 12:22:33: 36000000 INFO @ Tue, 16 Jun 2020 12:22:39: 27000000 INFO @ Tue, 16 Jun 2020 12:22:40: 33000000 INFO @ Tue, 16 Jun 2020 12:22:43: 37000000 INFO @ Tue, 16 Jun 2020 12:22:50: 28000000 INFO @ Tue, 16 Jun 2020 12:22:50: 34000000 INFO @ Tue, 16 Jun 2020 12:22:54: 38000000 INFO @ Tue, 16 Jun 2020 12:23:00: 35000000 INFO @ Tue, 16 Jun 2020 12:23:01: 29000000 INFO @ Tue, 16 Jun 2020 12:23:04: 39000000 INFO @ Tue, 16 Jun 2020 12:23:10: 36000000 INFO @ Tue, 16 Jun 2020 12:23:12: 30000000 INFO @ Tue, 16 Jun 2020 12:23:14: 40000000 INFO @ Tue, 16 Jun 2020 12:23:20: 37000000 INFO @ Tue, 16 Jun 2020 12:23:23: 31000000 INFO @ Tue, 16 Jun 2020 12:23:24: 41000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 12:23:31: 38000000 INFO @ Tue, 16 Jun 2020 12:23:34: 32000000 INFO @ Tue, 16 Jun 2020 12:23:34: 42000000 INFO @ Tue, 16 Jun 2020 12:23:41: 39000000 INFO @ Tue, 16 Jun 2020 12:23:45: 43000000 INFO @ Tue, 16 Jun 2020 12:23:45: 33000000 INFO @ Tue, 16 Jun 2020 12:23:51: 40000000 INFO @ Tue, 16 Jun 2020 12:23:55: 44000000 INFO @ Tue, 16 Jun 2020 12:23:57: 34000000 INFO @ Tue, 16 Jun 2020 12:24:01: 41000000 INFO @ Tue, 16 Jun 2020 12:24:05: 45000000 INFO @ Tue, 16 Jun 2020 12:24:09: 35000000 INFO @ Tue, 16 Jun 2020 12:24:12: 42000000 INFO @ Tue, 16 Jun 2020 12:24:16: 46000000 INFO @ Tue, 16 Jun 2020 12:24:20: 36000000 INFO @ Tue, 16 Jun 2020 12:24:22: 43000000 INFO @ Tue, 16 Jun 2020 12:24:26: 47000000 INFO @ Tue, 16 Jun 2020 12:24:31: 37000000 INFO @ Tue, 16 Jun 2020 12:24:32: 44000000 INFO @ Tue, 16 Jun 2020 12:24:36: 48000000 INFO @ Tue, 16 Jun 2020 12:24:43: 38000000 INFO @ Tue, 16 Jun 2020 12:24:43: 45000000 INFO @ Tue, 16 Jun 2020 12:24:47: 49000000 INFO @ Tue, 16 Jun 2020 12:24:53: 46000000 INFO @ Tue, 16 Jun 2020 12:24:54: 39000000 INFO @ Tue, 16 Jun 2020 12:24:57: 50000000 INFO @ Tue, 16 Jun 2020 12:25:04: 47000000 INFO @ Tue, 16 Jun 2020 12:25:06: 40000000 INFO @ Tue, 16 Jun 2020 12:25:08: 51000000 INFO @ Tue, 16 Jun 2020 12:25:14: 48000000 INFO @ Tue, 16 Jun 2020 12:25:17: 41000000 INFO @ Tue, 16 Jun 2020 12:25:19: 52000000 INFO @ Tue, 16 Jun 2020 12:25:25: 49000000 INFO @ Tue, 16 Jun 2020 12:25:29: 42000000 INFO @ Tue, 16 Jun 2020 12:25:29: 53000000 INFO @ Tue, 16 Jun 2020 12:25:36: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 12:25:36: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 12:25:36: #1 total tags in treatment: 12888218 INFO @ Tue, 16 Jun 2020 12:25:36: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 12:25:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 12:25:36: 50000000 INFO @ Tue, 16 Jun 2020 12:25:36: #1 tags after filtering in treatment: 10822535 INFO @ Tue, 16 Jun 2020 12:25:36: #1 Redundant rate of treatment: 0.16 INFO @ Tue, 16 Jun 2020 12:25:36: #1 finished! INFO @ Tue, 16 Jun 2020 12:25:36: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 12:25:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 12:25:37: #2 number of paired peaks: 299 WARNING @ Tue, 16 Jun 2020 12:25:37: Fewer paired peaks (299) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 299 pairs to build model! INFO @ Tue, 16 Jun 2020 12:25:37: start model_add_line... INFO @ Tue, 16 Jun 2020 12:25:37: start X-correlation... INFO @ Tue, 16 Jun 2020 12:25:37: end of X-cor INFO @ Tue, 16 Jun 2020 12:25:37: #2 finished! INFO @ Tue, 16 Jun 2020 12:25:37: #2 predicted fragment length is 212 bps INFO @ Tue, 16 Jun 2020 12:25:37: #2 alternative fragment length(s) may be 212 bps INFO @ Tue, 16 Jun 2020 12:25:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.05_model.r WARNING @ Tue, 16 Jun 2020 12:25:37: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 12:25:37: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Tue, 16 Jun 2020 12:25:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 12:25:37: #3 Call peaks... INFO @ Tue, 16 Jun 2020 12:25:37: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 12:25:40: 43000000 INFO @ Tue, 16 Jun 2020 12:25:46: 51000000 INFO @ Tue, 16 Jun 2020 12:25:52: 44000000 INFO @ Tue, 16 Jun 2020 12:25:57: 52000000 INFO @ Tue, 16 Jun 2020 12:26:00: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:26:03: 45000000 INFO @ Tue, 16 Jun 2020 12:26:07: 53000000 INFO @ Tue, 16 Jun 2020 12:26:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.05_peaks.xls INFO @ Tue, 16 Jun 2020 12:26:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:26:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.05_summits.bed INFO @ Tue, 16 Jun 2020 12:26:11: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (556 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 12:26:13: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 12:26:13: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 12:26:13: #1 total tags in treatment: 12888218 INFO @ Tue, 16 Jun 2020 12:26:13: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 12:26:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 12:26:14: #1 tags after filtering in treatment: 10822535 INFO @ Tue, 16 Jun 2020 12:26:14: #1 Redundant rate of treatment: 0.16 INFO @ Tue, 16 Jun 2020 12:26:14: #1 finished! INFO @ Tue, 16 Jun 2020 12:26:14: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 12:26:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 12:26:14: #2 number of paired peaks: 299 WARNING @ Tue, 16 Jun 2020 12:26:14: Fewer paired peaks (299) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 299 pairs to build model! INFO @ Tue, 16 Jun 2020 12:26:14: start model_add_line... INFO @ Tue, 16 Jun 2020 12:26:14: start X-correlation... INFO @ Tue, 16 Jun 2020 12:26:14: end of X-cor INFO @ Tue, 16 Jun 2020 12:26:14: #2 finished! INFO @ Tue, 16 Jun 2020 12:26:14: #2 predicted fragment length is 212 bps INFO @ Tue, 16 Jun 2020 12:26:14: #2 alternative fragment length(s) may be 212 bps INFO @ Tue, 16 Jun 2020 12:26:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.10_model.r WARNING @ Tue, 16 Jun 2020 12:26:14: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 12:26:14: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Tue, 16 Jun 2020 12:26:14: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 12:26:14: #3 Call peaks... INFO @ Tue, 16 Jun 2020 12:26:14: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 12:26:15: 46000000 INFO @ Tue, 16 Jun 2020 12:26:25: 47000000 INFO @ Tue, 16 Jun 2020 12:26:35: 48000000 INFO @ Tue, 16 Jun 2020 12:26:38: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:26:45: 49000000 INFO @ Tue, 16 Jun 2020 12:26:49: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.10_peaks.xls INFO @ Tue, 16 Jun 2020 12:26:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:26:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.10_summits.bed INFO @ Tue, 16 Jun 2020 12:26:49: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (367 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 12:26:56: 50000000 INFO @ Tue, 16 Jun 2020 12:27:06: 51000000 INFO @ Tue, 16 Jun 2020 12:27:16: 52000000 INFO @ Tue, 16 Jun 2020 12:27:26: 53000000 INFO @ Tue, 16 Jun 2020 12:27:32: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 12:27:32: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 12:27:32: #1 total tags in treatment: 12888218 INFO @ Tue, 16 Jun 2020 12:27:32: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 12:27:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 12:27:33: #1 tags after filtering in treatment: 10822535 INFO @ Tue, 16 Jun 2020 12:27:33: #1 Redundant rate of treatment: 0.16 INFO @ Tue, 16 Jun 2020 12:27:33: #1 finished! INFO @ Tue, 16 Jun 2020 12:27:33: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 12:27:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 12:27:33: #2 number of paired peaks: 299 WARNING @ Tue, 16 Jun 2020 12:27:33: Fewer paired peaks (299) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 299 pairs to build model! INFO @ Tue, 16 Jun 2020 12:27:33: start model_add_line... INFO @ Tue, 16 Jun 2020 12:27:33: start X-correlation... INFO @ Tue, 16 Jun 2020 12:27:33: end of X-cor INFO @ Tue, 16 Jun 2020 12:27:33: #2 finished! INFO @ Tue, 16 Jun 2020 12:27:33: #2 predicted fragment length is 212 bps INFO @ Tue, 16 Jun 2020 12:27:33: #2 alternative fragment length(s) may be 212 bps INFO @ Tue, 16 Jun 2020 12:27:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.20_model.r WARNING @ Tue, 16 Jun 2020 12:27:33: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 12:27:33: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Tue, 16 Jun 2020 12:27:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 12:27:33: #3 Call peaks... INFO @ Tue, 16 Jun 2020 12:27:33: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 12:27:56: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:28:08: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.20_peaks.xls INFO @ Tue, 16 Jun 2020 12:28:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:28:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619583/SRX6619583.20_summits.bed INFO @ Tue, 16 Jun 2020 12:28:08: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (228 records, 4 fields): 1 millis CompletedMACS2peakCalling